LAT is essential for the mast cell stabilising effect of tHGA in IgE-mediated mast cell activation by Tan, Ji Wei, Israf, Daud Ahmad, Md Hashim, Nur Fariesha, Cheah, Yoke Kqueen, Harith, Hanis Hazeera, Shaari, Khozirah, Tham, Chau Ling

“…In order to identify the molecular target of tHGA in IgE-mediated mast cell activation, the LAT and LAT2 genes in RBL-2H3 cells were knocked-down by using RNA interference to establish a LAT/LAT2 competition model. …”
Get full text

Civic engagement and social wellbeing among aged people in Ahwaz City, Iran by Asadollahi, Abdolrahim

“…The theories used include social well-being theory of Keyes and Shapiro (1998-2009), civic engagement in the theories of Putnam and Knock (2002), and activity theory. Also, demographic-individual inquiries were used i.e. …”
Get full text

Parametric performance and operational characteristics of motor gasoline fuel using Lycoming o-320 aviation engine by Kumar, Thanikasalam

“…Brake horsepower (BHP), brake thermal efficiency (BTHE), brake specific fuel consumption (BSFC), exhaust gas temperature (EGT), relative knock index (RKI), carbon dioxide (CO2), carbon monoxide (CO), unburned hydrocarbon (UHC) and nitrogen oxide (NOx) were recorded during the experiments. …”
Get full text

Network pharmacology and experimental analysis to reveal the mechanism of Dan-Shen-Yin against endothelial to mesenchymal transition in atherosclerosis by Mengyun Hong, Yubiao Wu, Haiyi Zhang, Jinchao Gu, Juanjuan Chen, Yancheng Guan, Xiude Qin, Yu Li, Jiahui Cao

“…LASP1, which is upstream of the PI3K/AKT pathway, had strong binding affinity to the majority of DSY’s bioactive ingredients, was induced by EndMT-promoting stimuli involving IL-1β, TGF-β2, and hypoxia, and was downregulated by DSY. Knock-down of LASP1 attenuated the expression of integrin αV, integrin β1, PI3K, AKT1 and EndMT-related genes induced by TGF-β2, and minimized the effect of DSY. …”
Get full text

Identification and validation of UBE2B as a prognostic biomarker promoting the development of esophageal carcinomas by Han Ding, Han Ding, Jia-Cheng Xu, Zhi-Guo Ding, Lin-Feng Wu, Lin-Feng Wu, Yan-Bo Liu, Yan-Bo Liu, Yi-Fei Zhang, Yi-Fei Zhang, Tian-Yin Chen, Tian-Yin Chen, Yi-Qun Zhang, Yi-Qun Zhang, Ping-Hong Zhou, Ping-Hong Zhou

“…Notably, proliferation and migration in ESCA cells were effectively suppressed when the expression of UBE2B was knocked down.ConclusionsTo summarize, this study has made a discovery regarding the importance of gaining new insights into the role of UBE2B in ESCA. …”
Get full text

Translocation of gasdermin D induced mitochondrial injury and mitophagy mediated quality control in lipopolysaccharide related cardiomyocyte injury by Ziqing Yu, Zilong Xiao, Lichun Guan, Pei Bao, Yong Yu, Yixiu Liang, Minghui Li, Zhenzhen Huang, Xueying Chen, Ruizhen Chen, Yangang Su, Junbo Ge

“…Besides, mitophagy alleviating GSDMD‐N induced mitochondrial injury was proved by pre‐treatment of autophagy antagonist or agonist in GSDMD‐knock out or GSDMD‐overexpression cells. A time‐dependent pattern of GSDMD translocation and mitochondrial GSDMD targeted mitophagy were verified. …”
Get full text

HUCMSC‐derived Exosomes Suppress the Titanium Particles‐induced Osteolysis in Mice through Inhibiting CCL2 and CCL3 by Shixun Li, Chuangran Wu, Sipeng Lin, Zhenkang Wen, Wenqiang Luo, Changchuan Li, Xiaoyan Wang, Xuejia Li, Liangbin Gao, Yue Ding

“…Local injection of lentivirus that knocked down CCL2 or CCL3 in a cranial osteolysis mice model were used to exam the effect of CCL2 and CCL3 on titanium particles‐induced osteolysis in vivo. …”
Get full text

The role of CXCL2-mediated crosstalk between tumor cells and macrophages in Fusobacterium nucleatum-promoted oral squamous cell carcinoma progression by Fujiao Nie, Jie Zhang, Haoyang Tian, Jingjing Zhao, Pizhang Gong, Huiru Wang, Suli Wang, Pishan Yang, Chengzhe Yang

“…Suppression of CXCL2 in OSCC cells weakened Fn’s promoting effects on OSCC cell proliferation, migration, macrophage recruitment, and M2 polarization. Conversely, knocking down CXCL2 in macrophages reversed the Fn-induced feedback effect of macrophages on the highly invasive phenotype of OSCC cells. …”
Get full text

Colour and melanopsin mediated responses in the murine retina by Joshua W. Mouland, Joshua W. Mouland, Alex J. Watson, Alex J. Watson, Franck P. Martial, Franck P. Martial, Robert J. Lucas, Robert J. Lucas, Timothy M. Brown, Timothy M. Brown

“…Indeed, there is still uncertainty around the overall prevalence of cone-dependent colour opponency across the mouse retina, given the strong retinal gradient in S and M-cone opsin (co)-expression and overlapping spectral sensitivities of most mouse opsins.Methods: To address this, we use photoreceptor isolating stimuli in multielectrode recordings from human red cone opsin knock-in mouse (Opn1mwR) retinas to systematically survey cone mediated responses and the occurrence of colour opponency across ganglion cell layer (GCL) neurons and identify ipRGCs based on spectral comparisons and/or the persistence of light responses under synaptic blockade.Results: Despite detecting robust cone-mediated responses across the retina, we find cone opponency is rare, especially outside of the central retina (overall ~3% of GCL neurons). …”
Get full text

Enhancing HIF-1α–P2X2 signaling in dorsal raphe serotonergic neurons promotes psychological resilience by Yuan Zhang, Yi-da Pan, Wen-ying Zheng, Huan-yu Li, Min-zhen Zhu, Wen-jie Ou yang, Yu Qian, Gustavo Turecki, Naguib Mechawar, Xin-hong Zhu

“…HIF-1α translationally regulated the expression of P2X2, and conditionally knocking out P2rx2 (encodes P2X2 receptors) in DRN5−HT neurons, in turn, attenuated the sustained antidepressant-like effect of IHHT, but not its acute effect. …”
Get full text

Consequences of SUR2[A478V] Mutation in Skeletal Muscle of Murine Model of Cantu Syndrome by Rosa Scala, Fatima Maqoud, Nicola Zizzo, Giuseppe Passantino, Antonietta Mele, Giulia Maria Camerino, Conor McClenaghan, Theresa M. Harter, Colin G. Nichols, Domenico Tricarico

“…Histopathological and qPCR analysis revealed atrophy of soleus and tibialis anterior muscles and up-regulation of atrogin-1 and MuRF1 mRNA in CS mice. (4) Conclusions: SUR2[A478V] “knock-in” mutation in mice impairs KATP channel modulation by MgATP, markedly so in SUR2^AV/AV, with atrophy and non-inflammatory edema in different skeletal muscle phenotypes.…”
Get full text

S100A10 Has a Critical Regulatory Function in Mammary Tumor Growth and Metastasis: Insights Using MMTV-PyMT Oncomice and Clinical Patient Sample Analysis by Alamelu G. Bharadwaj, Margaret L. Dahn, Rong-Zong Liu, Patricia Colp, Lynn N. Thomas, Ryan W. Holloway, Paola A. Marignani, Catherine K. L. Too, Penelope J. Barnes, Roseline Godbout, Paola Marcato, David M. Waisman

“…The genetic deletion of p11 resulted in significantly decreased tumor onset, growth rate, and spontaneous pulmonary metastatic burden in the PyMT/p11-KO (knock-out) mice. This phenotype was accompanied by substantial reduction in Ki67 positivity, macrophage infiltration, decreased vascular density in the primary tumors, and decrease in invasive carcinoma and pulmonary metastasis. …”
Get full text

RGS20 promotes non-small cell lung carcinoma proliferation via autophagy activation and inhibition of the PKA-Hippo signaling pathway by Xiaoyan Ding, Xiaoxia Li, Yanxia Jiang, Yujun Li, Hong Li, Lipeng Shang, Guilin Feng, Huhu Zhang, Ziyuan Xu, Lina Yang, Bing Li, Robert Chunhua Zhao

“…Remarkably, inhibiting Hippo signaling with GA-017 promoted cell proliferation and activated autophagy in RGS20 knock-down cells. However, forskolin, a GPCR activator, increased YAP phosphorylation and reversed the promoting effect of RGS20 in RGS20-overexpressing cells. …”
Get full text

<i>Sh3bp2</i> Gain-Of-Function Mutation Ameliorates Lupus Phenotypes in B6.MRL-<i>Fas^lpr</i> Mice by Akiko Nagasu, Tomoyuki Mukai, Masanori Iseki, Kyoko Kawahara, Shoko Tsuji, Hajime Nagasu, Yasuyoshi Ueki, Katsuhiko Ishihara, Naoki Kashihara, Yoshitaka Morita

“…<i>Sh3bp2</i> gain-of-function (P416R knock-in; <i>Sh3bp2^KI/+</i>) mice and lupus-prone B6.MRL-<i>Fas^lpr</i> mice were crossed to yield double-mutant (<i>Sh3bp2^KI/+Fas^lpr/lpr</i>) mice. …”
Get full text

Gut microbiota-derived ursodeoxycholic acid alleviates low birth weight-induced colonic inflammation by enhancing M2 macrophage polarization by Yu Pi, Yujun Wu, Xiangyu Zhang, Dongdong Lu, Dandan Han, Jiangchao Zhao, Xiaojiao Zheng, Shiyi Zhang, Hao Ye, Shuai Lian, Yu Bai, Zhenyu Wang, Shiyu Tao, Dongjiao Ni, Xinhua Zou, Wei Jia, Guolong Zhang, Defa Li, Junjun Wang

“…Levels of farnesoid X receptor (FXR) were knocked down in J774A.1 cells with small interfering RNAs. …”
Get full text

Breast-Specific Epigenetic Regulation of DeltaNp73 and Its Role in DNA-Damage-Response of <i>BRCA1</i>-Mutated Human Mammary Epithelial Cells by Ayelet Avraham, Susanna Feldman, Sean Soonweng Cho, Ayala Kol, Lior Heler, Emmanuela Riklin-Nahmias, Avishay Sella, Tamar Karni, Tanir M. Allweis, Saraswati Sukumar, Ella Evron

“…Notably, when BRCA1 was knocked down in MCF10A, a non-malignant breast epithelial cell line, both deltaNp73 induction and its protective effect from cell death were augmented upon DNA damage. …”
Get full text

Loxhd1b inhibits the hair cell development in zebrafish: Possible relation to the BDNF/TrkB/ERK pathway by Jingwen Liu, Jingwen Liu, Xu Zhang, Xu Zhang, Qingchen Zhang, Rongrong Wang, Jingyu Ma, Xiaohui Bai, Dawei Wang, Dawei Wang

“…Confocal microscopic in vivo imaging was used to detect the number and morphological characteristics of lateral line neuromasts and inner ear hair cells in zebrafish that knocked down Loxhd1b gene. The effect of knockdown Loxhd1b gene on the development of zebrafish otolith and semicircular canal was observed using microscopic. …”
Get full text

LOXL2-dependent deacetylation of aldolase A induces metabolic reprogramming and tumor progression by Ji-Wei Jiao, Xiu-Hui Zhan, Juan-Juan Wang, Li-Xia He, Zhen-Chang Guo, Xiu-E Xu, Lian-Di Liao, Xin Huang, Bing Wen, Yi-Wei Xu, Hai Hu, Gera Neufeld, Zhi-Jie Chang, Kai Zhang, Li-Yan Xu, En-Min Li

“…Consistently, integrated transcriptomic and metabolomic analysis of a knock-in mouse model expressing L2Δ13 gene revealed that LOXL2/L2Δ13 overexpression perturbs glucose and lipid metabolism. …”
Get full text

MicroRNA-542-3p targets Pten to inhibit the myoblasts proliferation but suppresses myogenic differentiation independent of targeted Pten by Dandan Li, Yongqi Yue, Xinxin Feng, Weibing Lv, Yilin Fan, Peiran Sha, Te Zhao, Yaqiu Lin, Xianrong Xiong, Jian Li, Yan Xiong

“…Results The data shows overexpression of miR-542-3p downregulation of mRNA and protein levels of proliferation marker genes, reduction of EdU+ cells, and cellular vitality. Additionally, knocking it down promoted the aforementioned phenotypes. …”
Get full text

New Insights into the Behavior of NHC-Gold Complexes in Cancer Cells by Giuseppa Augello, Antonina Azzolina, Filomena Rossi, Filippo Prencipe, Giuseppe Felice Mangiatordi, Michele Saviano, Luisa Ronga, Melchiorre Cervello, Diego Tesauro

“…At the molecular level, Au3BC induced a protective response mechanism in Huh7 and MDA-MB-231 cells, by inducing up-regulation of RAD51 and p62 protein expression, two proteins involved in DNA damage repair and autophagy, respectively. <i>RAD51</i> gene knock-down in HCC cells increased cell sensitivity to Au3BC by significant reduction of cell viability, induction of DNA damage, and induction of apoptosis and autophagy. …”
Get full text