ASPH-notch Axis guided Exosomal delivery of Prometastatic Secretome renders breast Cancer multi-organ metastasis by Qiushi Lin, Xuesong Chen, Fanzheng Meng, Kosuke Ogawa, Min Li, Ruipeng Song, Shugeng Zhang, Ziran Zhang, Xianglu Kong, Qinggang Xu, Fuliang He, Xuewei Bai, Bei Sun, Mien-Chie Hung, Lianxin Liu, Jack Wands, Xiaoqun Dong

“…Methods Stable cell lines overexpressing or knocking-out of ASPH were established using lentivirus transfection or CRISPR-CAS9 systems. …”
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Annexin A1 Is Required for Efficient Tumor Initiation and Cancer Stem Cell Maintenance in a Model of Human Breast Cancer by Cameron N. Johnstone, Yan Tu, Shenna Langenbach, David Baloyan, Andrew D. Pattison, Peter Lock, Kara L. Britt, Brian D. Lehmann, Traude H. Beilharz, Matthias Ernst, Robin L. Anderson, Alastair G. Stewart

“…Annexin A1 was stably knocked down in a panel of human and murine TNBC cell lines with high endogenous Annexin A1 expression that were then evaluated for orthotopic growth and spontaneous metastasis in vivo and for alterations in cell morphology in vitro. …”
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IFN regulatory factor 3 of golden pompano and its NLS domain are involved in antibacterial innate immunity and regulate the expression of type I interferon (IFNa3) by Yun Sun, Yun Sun, Zhenjie Cao, Zhenjie Cao, Panpan Zhang, Panpan Zhang, Caoying Wei, Caoying Wei, Jianlong Li, Jianlong Li, Ying Wu, Ying Wu, Yongcan Zhou, Yongcan Zhou

“…By overexpressing TroIRF3, the fish were able to inhibit the replication of V. harveyi, whereas knocking it down increased bacterial replication. Moreover, the overexpression of TroIRF3 increased type I interferon (IFNa3) production and the IFN signalling molecules. …”
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Modifying Escherichia coli to mimic Shigella for medical microbiology laboratory teaching: a new strategy to improve biosafety in class by Guangyuan Zhang, Guangyuan Zhang, Jia Liu, Yonglin He, Yuheng Du, Lei Xu, Tingting Chen, Yanan Guo, Huichao Fu, Anlong Li, Yunbo Tian, Yan Hu, Chun Yang, Mingqi Lu, Xichuan Deng, Jingsong Wang, Nan Lu

“…This study describes the modification of Escherichia coli DH5α to mimic Shigella and its evaluation as a safe alternative for medical laboratory teaching.MethodsTo generate E. coli DH5α△FliC△tnaA2a, the tnaA and FliC genes in E. coli DH5α were knocked out using CRISPR/Cas9 technology; a plasmid bearing the O-antigen determinant of S. flexneri 2a was then constructed and transformed. …”
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AMP‐activated protein kinase inhibition in fibro‐adipogenic progenitors impairs muscle regeneration and increases fibrosis by Xiangdong Liu, Liang Zhao, Yao Gao, Yanting Chen, Qiyu Tian, Jun Seok Son, Song Ah Chae, Jeanene Marie deAvila, Mei‐Jun Zhu, Min Du

“…Methods To test, AMPKα1fl/fl PDGFRαCre mice were used to knock out AMPKα1 in FAPs. Following AMPKα1 knockout, the mice were injected with phosphate‐buffered saline or glycerol to induce muscle injury. …”
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MdWRKY40is directly promotes anthocyanin accumulation and blocks MdMYB15L, the repressor of MdCBF2, which improves cold tolerance in apple by Peng-yue XU, Li XU, Hai-feng XU, Xiao-wen HE, Ping HE, Yuan-sheng CHANG, Sen WANG, Wen-yan ZHENG, Chuan-zeng WANG, Xin CHEN, Lin-guang LI, Hai-bo WANG

“…When the LZ of MdWRMY40is was knocked out, MdWRKY40is overexpression in the callus did not affect MdCBF2 expression or callus cold tolerance, indicating that MdWRKY40is acts in the cold signaling pathway by interacting with MdMYB15L. …”
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Presenilin-1 F105C mutation leads to tau accumulation in human neurons via the Akt/mTORC1 signaling pathway by Cheong-Meng Chong, Yuan Tan, Jiaqi Tong, Minjing Ke, Ke Zhang, Lingli Yan, Xiaotong Cen, Jia-Hong Lu, Guobing Chen, Huanxing Su, Dajiang Qin

“…Methods We generated heterozygotes and homozygotes of PS1 F105C knock-in mutation in human induced pluripotent stem cells (iPSCs) via CRISPR/Cas9/piggyback-based gene editing and differentiated them into human neurons. …”
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Combinatorial targeting of menin and the histone methyltransferase DOT1L as a novel therapeutic strategy for treatment of chemotherapy-resistant ovarian cancer by Elena Alexandrova, Jessica Lamberti, Domenico Memoli, Claudia Quercia, Viola Melone, Francesca Rizzo, Roberta Tarallo, Giorgio Giurato, Giovanni Nassa, Alessandro Weisz

“…Methods siRNA-mediated gene knock-down and pharmacological inhibition of menin, a key component of the MLL/SET1 complex and a fitness gene in OC cells, coupled to cell proliferation assays on a panel of high grade serous OC cell lines, including chemotherapy-sensitive and -resistant clones, were applied in order to evaluate how depletion or blockade of this enzyme influences growth and viability of OC cells. …”
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Metabolic engineering of Pichia pastoris for myo-inositol production by dynamic regulation of central metabolism by Qiquan Zhang, Xiaolu Wang, Huiying Luo, Yaru Wang, Yuan Wang, Tao Tu, Xing Qin, Xiaoyun Su, Huoqing Huang, Bin Yao, Yingguo Bai, Jie Zhang

“…Through enhancing native inositol biosynthesis pathway, knocking out inositol transporters, and slowing down carbon flux of glycolysis, an inositol-producing mutant was successfully developed and low inositol production of 0.71 g/L was obtained. …”
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The Role of Lactate Metabolism in Prostate Cancer Progression and Metastases Revealed by Dual-Agent Hyperpolarized ¹³C MRSI by Robert Bok, Jessie Lee, Renuka Sriram, Kayvan Keshari, Subramaniam Sukumar, Saeed Daneshmandi, David E. Korenchan, Robert R. Flavell, Daniel B. Vigneron, John Kurhanewicz, Pankaj Seth

“…This study applied a dual-agent, ¹³C-pyruvate and ¹³C-urea, hyperpolarized ¹³C magnetic resonance spectroscopic imaging (MRSI) and multi-parametric (mp) ¹H magnetic resonance imaging (MRI) approach in the transgenic adenocarcinoma of mouse prostate (TRAMP) model to investigate changes in tumor perfusion and lactate metabolism during prostate cancer development, progression and metastases, and after lactate dehydrogenase-A (LDHA) knock-out. An increased Warburg effect, as measured by an elevated hyperpolarized (HP) Lactate/Pyruvate (Lac/Pyr) ratio, and associated <i>Ldha</i> expression and LDH activity were significantly higher in high- versus low-grade TRAMP tumors and normal prostates. …”
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<i>POPDC1</i> Variants Cause Atrioventricular Node Dysfunction and Arrhythmogenic Changes in Cardiac Electrophysiology and Intracellular Calcium Handling in Zebrafish by Matthew R. Stoyek, Sarah E. Doane, Shannon E. Dallaire, Zachary D. Long, Jessica M. Ramia, Donovan L. Cassidy-Nolan, Kar-Lai Poon, Thomas Brand, T. Alexander Quinn

“…To investigate the impact of POPDC1 dysfunction on cardiac electrophysiology and intracellular calcium handling, homozygous <i>popdc1^S191F</i> and <i>popdc1</i> knock-out (<i>popdc1^KO</i>) zebrafish larvae and adult isolated <i>popdc1^S191F</i> hearts were studied by functional fluorescent analysis. …”
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Daily changes on seasonal ecophysiological responses of the intertidal brown macroalga Lessonia spicata: Implications of climate change by Paula S. M. Celis-Plá, Andres Trabal, Andres Trabal, Camilo Navarrete, Camilo Navarrete, Macarena Troncoso, Macarena Troncoso, Fabiola Moenne, Antonio Zúñiga, Félix L. Figueroa, Claudio A. Sáez, Claudio A. Sáez

“…Shifts in dominance between primary producers that can capitalize on carbon availability for photosynthesis will have knock-on effects on marine ecosystems, affecting their ecophysiological responses and biological processes. …”
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Circular RNA PVT1 Regulates Cell Proliferation, Migration, and Apoptosis by Stabilizing c-Myc and Downstream Target CXCR4 Expression in Acute Myeloid Leukemia by Xian Fu Sheng, Li Li Hong, Lei Fan, Kai-Li Chen, Jie Mu, Si Yu Shen, Hai-Feng Zhuang

“…Cell viability and migration were inhibited and apoptosis was increased when circPVT1 was knocked down in THP-1 cells. Knockdown of circPVT1 resulted in marked suppression of c-Myc protein with no significant change in mRNA levels. …”
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Clinical Staphylococcus aureus inhibits human T-cell activity through interaction with the PD-1 receptor by Maiken Mellergaard, Sarah Line Skovbakke, Stine Dam Jepsen, Nafsika Panagiotopoulou, Amalie Bøge Rud Hansen, Weihua Tian, Astrid Lund, Rikke Illum Høgh, Sofie Hedlund Møller, Romain Guérillot, Ashleigh S. Hayes, Lise Tornvig Erikstrup, Lars Andresen, Anton Y. Peleg, Anders Rhod Larsen, Timothy P. Stinear, Aase Handberg, Christian Erikstrup, Benjamin P. Howden, Steffen Goletz, Dorte Frees, Søren Skov

“…Specificity of the interaction was confirmed using recombinant PD-1, as well as PD-1 overexpressing and knock out cells. Moreover, the PD-1-binding S. aureus inhibited intracellular calcium mobilization, T-cell proliferation, CD25 expression, and IL-2 secretion, while the key effects were alleviated by antibody-mediated PD-1 blockade using an engineered IgG1-based anti-PD-1 antibody. …”
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Epigenetic Targeting to Overcome Radioresistance in Head and Neck Cancer by Iñaki Schniewind, Maria José Besso, Sebastian Klicker, Franziska Maria Schwarz, Wahyu Wijaya Hadiwikarta, Susan Richter, Steffen Löck, Annett Linge, Mechthild Krause, Anna Dubrovska, Michael Baumann, Ina Kurth, Claudia Peitzsch

“…The clonogenic survival assay after siRNA-mediated knock-down of both genes radiosensitized Cal33 and SAS cell lines. …”
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Paraoxonase-1 Regulation of Renal Inflammation and Fibrosis in Chronic Kidney Disease by Fatimah K. Khalaf, Chrysan J. Mohammed, Prabhatchandra Dube, Jacob A. Connolly, Apurva Lad, Usman M. Ashraf, Joshua D. Breidenbach, Robin C. Su, Andrew L. Kleinhenz, Deepak Malhotra, Amira F. Gohara, Steven T. Haller, David J. Kennedy

“…Experiments were performed on control Dahl salt-sensitive rats (SS^Mcwi, hereafter designated SS rats) and Pon1 knock-out rats (designated SS-Pon1^em1Mcwi, hereafter designated SS-PON-1 KO rats) generated by injecting a CRISPR targeting the sequence into SS^Mcwi rat embryos. …”
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Phenotypic effects of concomitant insensitive acetylcholinesterase (ace-1 R ) and knockdown resistance (kdr R ) in Anopheles gambiae: a hindrance for insecticide resistance managem... by Benoît S Assogba, Luc S Djogbénou, Jacques Saizonou, Pascal Milesi, Laurette Djossou, Innocent Djegbe, Welbeck A Oumbouke, Fabrice Chandre, Lamine Baba-Moussa, Mylene Weill, Michel Makoutodé

“…For adult bioassays, the percentages of mosquitoes knocked down were significantly lower for ACERKDRKIS than for KISKDR with permethrin (p = 0.003) but not with deltamethrin. …”
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Complex N-Linked Glycosylation: A Potential Modifier of Niemann–Pick Disease, Type C1 Pathology by Niamh X. Cawley, Anna T. Lyons, Daniel Abebe, Rachel Luke, Julia Yerger, Rebecca Telese, Christopher A. Wassif, Joan E. Bailey-Wilson, Forbes D. Porter

“…To test the importance of complex asparagine-linked glycosylation in NPC1 pathology, we generated NPC1 knock-out mice deficient in MGAT5, a key Golgi-resident glycosyl transferase involved in complex asparagine-linked glycosylation. …”
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Human PD-L1 overexpression decreases xenogeneic human T-cell immune responses towards porcine kidneys by Katharina Schmalkuche, Katharina Schmalkuche, Tamina Rother, Sevval Besli, Reinhard Schwinzer, Reinhard Schwinzer, Rainer Blasczyk, Björn Petersen, Björn Petersen, Constanca Figueiredo, Constanca Figueiredo

“…Different attempts to improve the survival of xenografts led to the generation of transgenic pigs expressing various combinations of human protective genes or knocked out for specific antigens. Currently, testing the efficiency of porcine organs carrying different genetic modifications in preventing xenogeneic immune responses completely relies on in vitro assays, humanized mouse models, or non-human primate transplantation models. …”
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Validation of Candidate Host Cell Entry Factors for Bovine Herpes Virus Type-1 Based on a Genome-Wide CRISPR Knockout Screen by Wenfang Spring Tan, Enguang Rong, Inga Dry, Simon Lillico, Andy Law, Paul Digard, Bruce Whitelaw, Robert G. Dalziel

“…We provide evidence that Polio Virus Receptor-related protein (PVRL2) serves as a receptor for BoHV-1, mediating more efficient entry than the previously identified Polio Virus Receptor (PVR). By knocking out two enzymes that catalyze HSPG chain elongation, HST2ST1 and GLCE, we further demonstrate the significance of HSPG in BoHV-1 entry. …”
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