Extracellular vesicles derived from hypoxia-preconditioned olfactory mucosa mesenchymal stem cells enhance angiogenesis via miR-612 by Lite Ge, Chengfeng Xun, Wenshui Li, Shengyu Jin, Zuo Liu, Yi Zhuo, Da Duan, Zhiping Hu, Ping Chen, Ming Lu

“…To explore the roles of miR-612, overexpression and knock-down experiments were performed using a mimic and inhibitor or agomir and antagomir of miR-612. …”
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ZFP36L2 Role in Thyroid Functionality by Francesco Albano, Valeria Tucci, Perry J. Blackshear, Carla Reale, Luca Roberto, Filomena Russo, Pina Marotta, Immacolata Porreca, Marco Colella, Massimo Mallardo, Mario de Felice, Concetta Ambrosino

“…<i>Nis</i>, <i>Pax8,</i> and <i>Nkx2</i>.1 mRNAs were also reduced in <i>Zfp36l2</i> knock-out thyrocytes in vitro (L2KO), in which we confirmed the increased apoptosis. …”
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Systematic Characterization of the Clinical Relevance of KPNA4 in Pancreatic Ductal Adenocarcinoma by Jingpiao Bao, Jingpiao Bao, Chaoliang Xu, Bin Li, Bin Li, Zengkai Wu, Zengkai Wu, Jie Shen, Jie Shen, Pengli Song, Pengli Song, Qi Peng, Qi Peng, Guoyong Hu, Guoyong Hu

“…Subsequently, KPNA4 was knocked down in PDAC cell lines, and CCK-8, colony formation and wound healing assays were performed to test the functions of KPNA4 in vitro. …”
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Central Precocious Puberty Caused by Novel Mutations in the Promoter and 5′-UTR Region of the Imprinted MKRN3 Gene by Pavlos Fanis, Pavlos Fanis, Nicos Skordis, Nicos Skordis, Meropi Toumba, Meropi Toumba, Nikoletta Papaioannou, Nikoletta Papaioannou, Anestis Makris, Andreas Kyriakou, Vassos Neocleous, Vassos Neocleous, Leonidas A. Phylactou, Leonidas A. Phylactou

“…GN11 cells were previously checked for Mkrn3 expression using lentivirus mediated knock-down. In silico analysis was implemented for the detection of changes in the mRNA secondary structure of the mutated MKRN3 5′-UTR.Results: Three novel heterozygous mutations (−166, −865, −886 nt upstream to the transcription start site) located in the proximal promoter region of the MKRN3 gene were identified in six non-related girls with CPP. …”
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Expression and Interaction Proteomics of GluA1- and GluA3-Subunit-Containing AMPARs Reveal Distinct Protein Composition by Sophie J. F. van der Spek, Nikhil J. Pandya, Frank Koopmans, Iryna Paliukhovich, Roel C. van der Schors, Mylene Otten, August B. Smit, Ka Wan Li

“…To reveal interactors belonging to specific AMPAR subcomplexes, we performed both expression and interaction proteomics on hippocampi of wildtype and <i>Gria1</i>- or <i>Gria3</i> knock-out mice. Whereas GluA1/2 receptors co-purified TARP-γ8, synapse differentiation-induced protein 4 (SynDIG4, also known as Prrt1) and CNIH-2 with highest abundances, GluA2/3 receptors revealed strongest co-purification of CNIH-2, TARP-γ2, and Noelin1 (or Olfactomedin-1). …”
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Double heterozygous pathogenic mutations in KIF3C and ZNF513 cause hereditary gingival fibromatosis by Jianfan Chen, Xueqing Xu, Song Chen, Ting Lu, Yingchun Zheng, Zhongzhi Gan, Zongrui Shen, Shunfei Ma, Duocai Wang, Leyi Su, Fei He, Xuan Shang, Huiyong Xu, Dong Chen, Leitao Zhang, Fu Xiong

“…ZNF513, a transcription factor, binds to KIF3C exon 1 and participates in the positive regulation of KIF3C expression in gingival fibroblasts. Furthermore, a knock-in mouse model confirmed that heterozygous or homozygous mutations within Zfp513 (p.R250W) or Kif3c (p.R412H) alone do not led to clear phenotypes with gingival fibromatosis, whereas the double mutations led to gingival hyperplasia phenotypes. …”
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TREM2 regulates microglial lipid droplet formation and represses post-ischemic brain injury by Wei Wei, Lin Zhang, Wenqiang Xin, Yongli Pan, Lars Tatenhorst, Zhongnan Hao, Stefan T. Gerner, Sabine Huber, Martin Juenemann, Marius Butz, Hagen B. Huttner, Mathias Bähr, Dirk Fitzner, Feng Jia, Thorsten R. Doeppner

“…Meanwhile, in a neuron-microglia co-culture system under hypoxic conditions, we found that microglia lost their protective effect against neuronal injury and apoptosis when TREM2 was knocked down. Under in vivo conditions, TREM2 knockdown mice express lower TGF-β1 expression levels and a lower number of anti-inflammatory M2 phenotype microglia, resulting in increased cerebral infarct size, exacerbated neuronal apoptosis, and aggravated neuronal impairment. …”
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β-amyloid accumulation enhances microtubule associated protein tau pathology in an APPNL-G-F/MAPTP301S mouse model of Alzheimer’s disease by Lulu Jiang, Lulu Jiang, Rebecca Roberts, Melissa Wong, Lushuang Zhang, Chelsea Joy Webber, Chelsea Joy Webber, Jenna Libera, Zihan Wang, Alper Kilci, Matthew Jenkins, Alejandro Rondón Ortiz, Luke Dorrian, Jingjing Sun, Jingjing Sun, Guangxin Sun, Sherif Rashad, Sherif Rashad, Caroline Kornbrek, Sarah Anne Daley, Sarah Anne Daley, Peter C. Dedon, Peter C. Dedon, Brian Nguyen, Weiming Xia, Weiming Xia, Takashi Saito, Takaomi C. Saido, Benjamin Wolozin, Benjamin Wolozin, Benjamin Wolozin, Benjamin Wolozin

“…IntroductionThe study of the pathophysiology study of Alzheimer’s disease (AD) has been hampered by lack animal models that recapitulate the major AD pathologies, including extracellular -amyloid (A) deposition, intracellular aggregation of microtubule associated protein tau (MAPT), inflammation and neurodegeneration.MethodsThe humanized APPNL-G-F knock-in mouse line was crossed to the PS19 MAPTP301S, over-expression mouse line to create the dual APPNL-G-F/PS19 MAPTP301S line. …”
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The garlic compound ajoene covalently binds vimentin, disrupts the vimentin network and exerts anti-metastatic activity in cancer cells by Catherine H. Kaschula, Rosanna Tuveri, Ellen Ngarande, Kevin Dzobo, Christopher Barnett, Daniel A. Kusza, Lisa M. Graham, Arieh A. Katz, Mohamed Suhail Rafudeen, M. Iqbal Parker, Roger Hunter, Georgia Schäfer

“…The invasion and migration activity was measured by wound healing and transwell assays using wildtype cells and cells in which the vimentin protein had been transiently knocked down by siRNA or overexpressed. Results The dominant protein tagged by dansyl-ajoene was identified to be the 57 kDa protein vimentin. …”
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Blockade of the lncRNA-DOT1L-LAMP5 axis enhances autophagy and promotes degradation of MLL fusion proteins by Tian-Qi Chen, Heng-Jing Huang, Shun-Xin Zhu, Xiao-Tong Chen, Ke-Jia Pu, Dan Wang, Yan An, Jun-Yi Lian, Yu-Meng Sun, Yue-Qin Chen, Wen-Tao Wang

“…LAMP5-AS1 acts as a novel autophagic suppressor, safeguarding MLL fusion proteins from autophagic degradation. Knocking down LAMP5-AS1 significantly induced apoptosis in MLL leukemia cell lines and primary cells and extended the survival of mice in vivo. …”
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Genomic surveillance of Anopheles mosquitoes on the Bijagós Archipelago using custom targeted amplicon sequencing identifies mutations associated with insecticide resistance by Sophie Moss, Elizabeth Pretorius, Sainey Ceesay, Harry Hutchins, Eunice Teixeira da Silva, Mamadou Ousmane Ndiath, Robert T. Jones, Hristina Vasileva, Jody Phelan, Holly Acford-Palmer, Emma Collins, Amabelia Rodrigues, Sanjeev Krishna, Taane G. Clark, Anna Last, Susana Campino

“…This study is the first to report the L995F knock-down resistance (kdr)-west allele in Anopheles melas on the Archipelago. …”
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Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research) by Frida Meijer Carlsen, Ida Elisabeth Johansen, Ida Elisabeth Johansen, Zhang Yang, Ying Liu, Ying Liu, Ida Nøhr Westberg, Nam Phuong Kieu, Bodil Jørgensen, Marit Lenman, Erik Andreasson, Kåre Lehmann Nielsen, Andreas Blennow, Bent Larsen Petersen

“…High editing efficiencies at the cell pool (protoplast) level are pivotal for achieving full allelic knock-out in tetraploids and for reducing the downstream cumbersome and delicate ex-plant regeneration. …”
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Vesicular Integral-Membrane Protein 36 Is Involved in the Selective Secretion of Fucosylated Proteins into Bile Duct-like Structures in HepG2 Cells by Mizuki Muranaka, Shinji Takamatsu, Tsunenori Ouchida, Yuri Kanazawa, Jumpei Kondo, Tsutomu Nakagawa, Yuriko Egashira, Koji Fukagawa, Jianguo Gu, Toru Okamoto, Yoshihiro Kamada, Eiji Miyoshi

“…Here, we aimed to identify cargo proteins involved in the selective secretion of fucosylated proteins, such as AFP-L3, into bile duct-like structures in HepG2 hepatoma cells, which have cellular polarity like, in part, normal hepatocytes. α1-6 Fucosyltransferase (FUT8) is a key enzyme to synthesize core fucose and produce AFP-L3. Firstly, we knocked out the <i>FUT8</i> gene in HepG2 cells and investigated the effects on the secretion of AFP-L3. …”
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IP3R2-mediated Ca2+ release promotes LPS-induced cardiomyocyte pyroptosis via the activation of NLRP3/Caspase-1/GSDMD pathway by Qing-Rui Wu, Hui Yang, Hui-Dan Zhang, Yong-Jiang Cai, Yan-Xiang Zheng, Heng Fang, Zi-Fan Wang, Su-Juan Kuang, Fang Rao, Huan-Lei Huang, Chun-Yu Deng, Chun-Bo Chen

“…Inhibiting IP3R activity with xestospongin C (XeC) or knocking down IP3R2 reversed LPS-induced intracellular Ca2+ release. …”
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Keratin 15 protects against cigarette smoke-induced epithelial mesenchymal transformation by MMP-9 by Wensi Zhu, Linxiao Han, Yuanyuan Wu, Lin Tong, Ludan He, Qin Wang, Yu Yan, Ting Pan, Jie Shen, Yuanlin Song, Yao Shen, Qiaoliang Zhu, Jian Zhou

“…Furthermore, lentivirus-delivered short hairpin RNA (shRNA) was used to knock down KRT15 in human bronchial epithelial (HBE) cells stimulated with cigarette smoke extract (CSE). …”
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WIPI2 enhances the vulnerability of colorectal cancer cells to erastin via bioinformatics analysis and experimental verification by Liying Yu, Yan Luo, Xile Ding, Miaomiao Tang, Huan Gao, Renfang Zhang, Mingfu Chen, Yuchen Liu, Yuchen Liu, Qiongxia Chen, Yanli Ouyang, Xiang Wang, Hongyan Zhen, Hongyan Zhen

“…Furthermore, we found that the expression level of ACSL4 decreased and that of GPX4 increased when WIPI2 was knocked down, suggesting that WIPI2 can potentially positively regulate CRC ferroptosis. …”
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Speckle-type POZ protein could play a potential inhibitory role in human renal cell carcinoma by Zhi Chen, Zuan Li, Chunlin Li, Bingcai Li, Haojian Wang, Deyong Nong, Ximing Li, Guihai Huang, Junhao Lin, Wei Li

“…Methods Here, SPOP was overexpressed by lentiviral vector transfection in ACHN and Caki-1 cells, and SPOP was knocked down in Caki-2 cells with similar transfection methods. …”
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The transcription factor TpRfx1 is an essential regulator of amylase and cellulase gene expression in Talaromyces pinophilus by Gui-Yan Liao, Shuai Zhao, Ting Zhang, Cheng-Xi Li, Lu-Sheng Liao, Feng-Fei Zhang, Xue-Mei Luo, Jia-Xun Feng

“…Thirty of the 33 genes were successfully knocked out in the parental strain T. pinophilus ∆TpKu70, with seven of the deletion mutants firstly displaying significant changes in amylase production as compared with the parental strain. …”
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A CRISPR/Cas9-mediated screen identifies determinants of early plasma cell differentiation by Ermeng Xiong, Oliver Popp, Claudia Salomon, Philipp Mertins, Christine Kocks, Christine Kocks, Klaus Rajewsky, Van Trung Chu, Van Trung Chu

“…After Hdac1 deletion plasmablast differentiation was consistently reduced by about 50%, while deletion of the closely related Hdac2 gene had no effect. Hdac1 knock-out led to strongly perturbed protein expression of antagonistic transcription factors that govern plasma cell versus B cell identity (by decreasing IRF4 and BLIMP1 and increasing BACH2 and PAX5).DiscussionTaken together, our results highlight specific and non-redundant roles for Ern1, Arf4 and Hdac1 in the early steps of plasma cell differentiation.…”
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<i>dCas9-BE</i>3 and <i>dCas12a-BE3</i> Systems Mediated Base Editing in Kiwifruit Canker Causal Agent <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> by Bo Liu, Wenpeng Song, Linchao Wang, Yantao Wu, Xiaoting Xu, Xiangli Niu, Shengxiong Huang, Yongsheng Liu, Wei Tang

“…In addition, a relatively saturated <i>Psa</i> gene knockout system covering more than 95% of genes was developed based on dCas9-BE3 and dCas12a-BE3, which could knock out two or three genes at the same time in the <i>Psa</i> genome. …”
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