Progesterone-induced progesterone receptor membrane component 1 rise-to-decline changes are essential for decidualization by Hailun Liu, André Franken, Alexandra P. Bielfeld, Tanja Fehm, Dieter Niederacher, Zhongping Cheng, Hans Neubauer, Nadia Stamm

“…Furthermore, we found that the interactions of prohibitin 1 and prohibitin 2 with PGRMC1 were induced upon progestin treatment. Knocking down each of the prohibitins slowed down the decidualization process compared to the control, suggesting that PGRMC1 cooperates with prohibitins to regulate decidualization. …”
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Prognostic prediction using a gene signature developed based on exhausted T cells for liver cancer patients by Yu Zhou, Wanrui Wu, Wei Cai, Dong Zhang, Weiwei Zhang, Yunling Luo, Fujing Cai, Zhenjing Shi

“…In vitro experiments revealed that the 7 risk genes all had a higher expression in HCC cells, and that both liver HCC cell numbers and cell viability were reduced by knocking down MMP-9. Conclusion: We developed a RiskScore model for predicting LIHC prognosis based on the scRNA-seq and RNA-seq data. …”
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The LXRB-SREBP1 network regulates lipogenic homeostasis by controlling the synthesis of polyunsaturated fatty acids in goat mammary epithelial cells by Wenying Zhang, Changhui Zhang, Jun Luo, Huifen Xu, Jianxin Liu, Juan J. Loor, Hengbo Shi

“…Luciferase reporter assays revealed that the activities of wild type SCD1 promoter or fragment with SREBP1 response element (SRE) mutation were decreased markedly when LXRB was knocked down. Activity of the SCD1 promoter that was induced by T09 was blocked when the SRE mutation was introduced. …”
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CRISPR/Cas9-mediated knockout of the Vanin-1 gene in the Leghorn Male Hepatoma cell line and its effects on lipid metabolism by Lu Xu, Zhongliang Wang, Shihao Liu, Zhiheng Wei, Jianfeng Yu, Jun Li, Jie Li, Wen Yao, Zhiliang Gu

“…Methods First, we detected the VNN1 mRNA expression in 4-week chickens which were fasted 24 hours. Next, knocked out VNN1 via CRISPR/Cas9 system in the chicken Leghorn Male Hepatoma cell line. …”
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Postural assessment of girls between 7 and 10 years of age Avaliação postural em meninas de 7 a 10 anos by Patrícia Jundi Penha, Sílvia Maria Amado João, Raquel Aparecida Casarotto, Carlos Junichiro Amino, Daniela Caielli Penteado

“…RESULTS: The main postural deviations found were knock-knee, medial rotation of the hip, antepulsion, pelvic anteversion, knee hyperextension, lumbar hyperlordosis, valgus ankle, imbalanced shoulders, lateral pelvic inclination, scoliosis, trunk rotation, thoracic hyperkyphosis, winged scapula, shoulder protraction, abducted scapula, medial rotation of shoulders, and head tilt. …”
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Thermotolerance improvement of engineered Saccharomyces cerevisiae ERG5 Delta ERG4 Delta ERG3 Delta, molecular mechanism, and its application in corn ethanol production by Peizhou Yang, Wenjing Wu, Jianchao Chen, Suwei Jiang, Zhi Zheng, Yanhong Deng, Jiuling Lu, Hu Wang, Yong Zhou, Yuyou Geng, Kanglin Wang

“…Results In this study, S. cerevisiae ERG5, ERG4, and ERG3 were knocked out using the CRISPR–Cas9 approach to impact the gene expression involved in ergosterol synthesis. …”
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Caveolin‐3 deficiency associated with the dystrophy P104L mutation impairs skeletal muscle mitochondrial form and function by Dinesh S. Shah, Raid B. Nisr, Clare Stretton, Gabriela Krasteva‐Christ, Harinder S. Hundal

“…These morphological, biochemical, and functional mitochondrial changes were phenocopied in myoblasts in which Cav3 had been silenced/knocked‐out using shRNA or CRISPR. Reduced mitochondrial mass, PGC1α, SDHA, ANT1, and MnSOD were also demonstrable in Cav3−/− mouse gastrocnemius. …”
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The PIK3CA E542K and E545K mutations promote glycolysis and proliferation via induction of the β-catenin/SIRT3 signaling pathway in cervical cancer by Wei Jiang, Tiancong He, Shuai Liu, Yingying Zheng, Libing Xiang, Xuan Pei, Ziliang Wang, Huijuan Yang

“…AKT/GSK3β/β-catenin signaling was highly activated in SiHa and MS751 cells with mutant PIK3CA. Knocking down β-catenin expression decreased glucose uptake and lactate production. …”
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Activation of cannabinoid-2 receptor protects against Pseudomonas aeruginosa induced acute lung injury and inflammation by Nagaraja Nagre, Gregory Nicholson, Xiaofei Cong, Janette Lockett, Andrew C. Pearson, Vincent Chan, Woong-Ki Kim, K. Yaragudri Vinod, John D. Catravas

“…These effects were abrogated by a CB2R antagonist, SR144528, further confirming the specificity of the CB2R-mediated effects. CB2R-knock out (CB2RKO) mice had a significantly higher level of PA-induced inflammation as compared to that in WT mice. …”
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Altered RBP1 Gene Expression Impacts Epithelial Cell Retinoic Acid, Proliferation, and Microenvironment by Jianshi Yu, Mariarita Perri, Jace W. Jones, Keely Pierzchalski, Natalia Ceaicovscaia, Erika Cione, Maureen A. Kane

“…Results show that RBP1 and atRA are reduced in mammary tumor tissue and tumorigenic epithelial cell lines. Knock down of RBP1 expression using shRNA or overexpression of RBP1 supported a direct relationship between RBP1 expression with atRA. …”
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Development of a diagnostic and risk prediction model for Alzheimer’s disease through integration of single-cell and bulk transcriptomic analysis of glutamine metabolism by Yan Guo, Yan Guo, Tingru Zhao, Tingru Zhao, Xi Chu, Xi Chu, Zhenyun Cheng, Zhenyun Cheng

“…RT-qPCR analysis revealed that the majority of the nine characteristic genes were differentially expressed in AD-induced rat neurons. Knocking down PHF1 could protect against neurite loss and alleviate cell injury in AD neurons. …”
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Analysis of co-isogenic prion protein deficient mice reveals behavioral deficits, learning impairment, and enhanced hippocampal excitability by A. Matamoros-Angles, A. Hervera, J. Soriano, E. Martí, P. Carulla, F. Llorens, M. Nuvolone, A. Aguzzi, I. Ferrer, A. Gruart, J. M. Delgado-García, J. A. Del Río

“…Such evidence has been confounded by the absence of an appropriate knock-out mouse model to dissect the biological relevance of PrPC, with some functions recently shown to be misattributed to PrPC due to the presence of genetic artifacts in mouse models. …”
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Nuclear Localization Sequence of FGF1 Is Not Required for Its Intracellular Anti-Apoptotic Activity in Differentiated Cells by Agata Lampart, Katarzyna Dominika Sluzalska, Aleksandra Czyrek, Aleksandra Szerszen, Jacek Otlewski, Antoni Wiedlocha, Malgorzata Zakrzewska

“…To verify the results obtained for NLS mutants, we knocked down LRRC59, a protein that mediates the nuclear transport of FGF1. …”
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LINC00478-derived novel cytoplasmic lncRNA LacRNA stabilizes PHB2 and suppresses breast cancer metastasis via repressing MYC targets by Rong Guo, Yonghui Su, Qi Zhang, Bingqiu Xiu, Sheng Huang, Weiru Chi, Liyi Zhang, Lun Li, Jianjing Hou, Jia Wang, Jiajian Chen, Yayun Chi, Jingyan Xue, Jiong Wu

“…Paired-guide CRISPR/cas9 and CRISPR/dead-Cas9 systems was used to knock out or activate the expression of LacRNA. Cell migration and invasion assay was performed to confirm the phenotype of LacRNA. …”
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Genome Editing of Rice <i>eIF4G</i> Loci Confers Partial Resistance to Rice Black-Streaked Dwarf Virus by Wei Wang, Shuhui Ma, Peng Hu, Yinghua Ji, Feng Sun

“…Furthermore, our data suggest that <i>eIF4G</i> may plays an important role in rice normal development, as there were no <i>eIF4G</i> knock-out homozygous mutants in T1 generation plants. …”
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Apigenin inhibits angiogenesis in retinal microvascular endothelial cells through regulating of the miR-140-5p/HDAC3-mediated PTEN/PI3K/AKT pathway by Chaojun Fu, Jun Peng, Yanjun Ling, Hongqing Zhao, Yongwang Zhao, Xiuli Zhang, Min Ai, Qinghua Peng, Yuhui Qin

“…HRMECs were treated with apigenin. Then we knocked down or overexpressed miR-140-5p and HDAC3, and added PI3K/AKT inhibitor LY294002. …”
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Enhancer Associated Long Non-coding RNA Transcription and Gene Regulation in Experimental Models of Rickettsial Infection by Imran H. Chowdhury, Hema P. Narra, Abha Sahni, Abha Sahni, Kamil Khanipov, Yuriy Fofanov, Sanjeev K. Sahni, Sanjeev K. Sahni

“…Finally, shRNA mediated knock-down of NONMMUT013718 and NONMMUT024103 elncRNAs resulted in reduced expression of endogenous Id2 and Apl10b, demonstrating the regulatory roles of these elncRNAs on their target PCGs. …”
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Oral Administration of Ginger-Derived Lipid Nanoparticles and Dmt1 siRNA Potentiates the Effect of Dietary Iron Restriction and Mitigates Pre-Existing Iron Overload in <i>Hamp</i>... by Xiaoyu Wang, Mingzhen Zhang, Regina R. Woloshun, Yang Yu, Jennifer K. Lee, Shireen R. L. Flores, Didier Merlin, James F. Collins

“…It was previously established that Dmt1 knock down prevented iron-loading in weanling <i>Hamp</i> (encoding hepcidin) KO mice (modeling type 2B HH). …”
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Low‐dose aspirin can inhibit exosomal release induced by radiotherapy in breast cancer and attenuate its inhibitory effect on NK cell proliferation by Li Wang, Zaoxiu Hu, Ceshi Chen, Ting Chen, Zhihong Yao, Wenhui Li, Zuozhang Yang

“…Low doses of aspirin inhibited exosome release from BT549 and 4T1‐Luc cells and alleviated the inhibitory effect of BC cell exosomes on NK cell proliferation. In addition, knocking down Rab27a reduced the protein levels of exosome‐related and secretion‐related genes in BC cells, further enhancing the promotive effect of aspirin on NK cell proliferation, while overexpressing Rab27a had the opposite effect. …”
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Auditory Phenotype and Histopathologic Findings of a Mutant Nlrp3 Expression Mouse Model by Yehree Kim, Sang-Yeon Lee, Min Young Kim, Kyusun Park, Jin Hee Han, Jung Ho Kim, Bong Jik Kim, Byung Yoon Choi

“…We aimed to establish a novel mouse model that manifests quantifiable hearing loss with other syndromic features due to alteration of Nlrp3 and investigate the audiologic and histopathologic phenotype in the cochlea to clarify how the genetic alterations of NLRP3 could induce autoinflammatory hearing loss.MethodsTo induce inner ear expression of the mutant Nlrp3, Nlrp3D301NneoR mice were bred with Gfi1Cre knock-in mice for conditional mutant Nlrp3 activation in the cochlea and hematopoietic cells. …”
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