Sumoylation-deficient phosphoglycerate mutase 2 impairs myogenic differentiation by Yi Zhang, Yi Zhang, Ilimbek Beketaev, Yanlin Ma, Yanlin Ma, Jun Wang

“…Given that K176 is more highly evolutionarily conserved across paralogs and orthologs than K49 is, we used the CRISPR-mediated homologous recombination technique in myogenic C2C12 cells to generate homozygous K176R knock-in cells (PGAM2K176R/K176R). Compared with wild-type (WT) C2C12 cells, PGAM2K176R/K176R C2C12 cells exhibited impaired myogenic differentiation, as indicated by decreased differentiation and fusion indexes. …”
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<i>Lactococcus lactis</i> subsp. <i>Cremoris</i> C60 restores T Cell Population in Small Intestinal Lamina Propria in Aged Interleukin-18 Deficient Mice by Suguru Saito, Nanae Kakizaki, Alato Okuno, Toshio Maekawa, Noriko M. Tsuji

“…We found that CD4+ T cells in the small intestine are dramatically decreased in aged Interleukin-18 knock out (IL-18KO) mice, associated with the impairment of IFN-γ production in the CD4+ T cells, especially in small intestinal lamina propria (LP). …”
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Experiment Study on Displacement Damage Effect on GaInP/GaAs/Ge Triple Junction Solar Cell Irradiated by 100 MeV Proton by WANG Zujun1;YIN Liyuan2;WANG Xinghong3;ZHANG Qi4;TANG Ning5;GUO Xiaoqiang1;SHENG Jiangkun1;GOU Shilong1;YAN Shixing5;LI Chuanzhou5

“…The information of the primary knocked-on atoms (PKAs) was obtained by judging the interaction between particles and materials in the transport process. …”

Therapeutic induction of Bcl2‐associated athanogene 3‐mediated autophagy in idiopathic pulmonary fibrosis by Shashipavan Chillappagari, Julian Schwarz, Vidyasagar Kesireddy, Jessica Knoell, Martina Korfei, Konrad Hoetzenecker, M. Lienhard Schmitz, Christian Behl, Saverio Bellusci, Andreas Guenther, Poornima Mahavadi

“…Such effects were observed upon therapeutic modulation of BAG3 but not upon knock down of BAG3 per se in IPF fibroblasts. Similarly, PCLS of IPF patients showed a significant decrease in collagen deposition in response to these drugs, either alone or in a more potent form in combination with Pirf. …”
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Role of Chromosome- and/or Plasmid-Located blaNDM on the Carbapenem Resistance and the Gene Stability in Escherichia coli by Noriko Sakamoto, Yukihiro Akeda, Yo Sugawara, Yuki Matsumoto, Daisuke Motooka, Tetsuya Iida, Shigeyuki Hamada

“…Escherichia coli M719 possessing both chromosomes- and plasmid-located blaNDM genes was used as a wild-type strain (WT) for the construction of mutants, ΔpblaNDM and ΔcblaNDM, wherein chromosome- or plasmid-located blaNDM, was knocked out, respectively. The mutant ΔpblaNDM showed lower hydrolyzing activity against imipenem and gene expression than the WT or ΔcblaNDM mutant. …”
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BAX activation in mouse retinal ganglion cells occurs in two temporally and mechanistically distinct steps by Margaret E. Maes, Ryan J. Donahue, Cassandra L. Schlamp, Olivia J. Marola, Richard T. Libby, Robert W. Nickells

“…The contribution of the Dual Leucine Zipper-JUN-N-Terminal Kinase cascade was evaluated using mice with a double conditional knock-out of both Mkk4 and Mkk7. Results ONC induces the translocation of GFP-BAX in RGCs at a slower rate and with less intracellular synchronicity than 661W cells, but exhibits less variability among mitochondrial foci within a single cell. …”
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Activation of CTNNB1 by deubiquitinase UCHL3-mediated stabilization facilitates bladder cancer progression by Tao Liu, Meng-Qi Fan, Xiao-Xiao Xie, Qi-Peng Shu, Xue-Hua Du, Lin-Zhi Qi, Xiao-Dong Zhang, Ming-Hui Zhang, Guang Shan, Run-Lei Du, Shang-Ze Li

“…Methods We utilized CRISPR‒Cas9 technology to construct cell lines with UCHL3 stably overexpressed or knocked out. The successful overexpression or knockout of UCHL3 was determined using Western blotting. …”
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Expression of glucose transporter-1 in follicular lymphoma affected tumor-infiltrating immunocytes and was related to progression of disease within 24 months by Yuwei Deng, Jianli Ma, Shu Zhao, Ming Yang, Yutian Sun, Qingyuan Zhang

“…The lactate production and the induction of M2 macrophages were detected when GLUT1 was transfected or knocked down in DOHH2. The activation of PI3K/Akt/mTOR signaling in DOHH2 and WSU-FSCCL cells co-cultured with induced inhibitory immunocytes was tracked by western blotting. …”
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Role of LGR5-positive mesenchymal cells in craniofacial development by Kristýna Olbertová, Kristýna Olbertová, Dušan Hrčkulák, Vítězslav Kříž, Wojciech Jesionek, Jan Kubovčiak, Milan Ešner, Vladimír Kořínek, Marcela Buchtová, Marcela Buchtová

“…To follow the fate of LGR5-positive cells, we performed lineage tracing using an inducible Cre knock-in allele in combination with Rosa26-tdTomato reporter mice. …”
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Transcriptional Effects of Rootstock on Scion after Drought: A Case Study of Using <i>MdGH3</i> RNAi as the Rootstock by Jieqiang He, Junxing Guo, Lijuan Jiang, Wenjing An, Fengwang Ma, Qingmei Guan, Chundong Niu

“…Using transgenic <i>MdGH3</i> RNAi (knocking down <i>MdGH3.6</i> and its five homologs) plants as rootstock can increase drought tolerance, water use efficiency, flowering, and fruit quality of the Fuji scion after drought stress. …”
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Epigenetic Regulation of MAP3K8 in EBV-Associated Gastric Carcinoma by Gaurab Roy, Ting Yang, Shangxin Liu, Yi-Ling Luo, Yuantao Liu, Qian Zhong

“…RNA-seq and pathway analysis indicated that knocking down <i>MAP3K8</i> obstructed the notch signaling pathway and epithelial-mesenchymal transition (EMT) in EBVaGC cells. …”
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The role of the desmosomal protein desmocollin 2 in tumour progression in triple negative breast cancer patients by Francesca Reimer, Sarah Bryan, Karen Legler, Thomas Karn, Serenella Eppenberger-Castori, Jakob Matschke, Thais Pereira-Veiga, Harriet Wikman, Isabell Witzel, Volkmar Müller, Barbara Schmalfeldt, Karin Milde-Langosch, Udo Schumacher, Christine Stürken, Leticia Oliveira-Ferrer

“…Functional consequences of DSC2 overexpression and DSC2 knock down were investigated in the triple negative breast cancer (TNBC) cell line MDA-MB-231 and its brain-seeking subline MDA-MB-231-BR, respectively in vitro and in vivo. …”
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Transcriptome-module phenotype association study implicates extracellular vesicles biogenesis in Plasmodium falciparum artemisinin resistance by Kwesi Z. Tandoh, Oheneba C. Hagan, Michael D. Wilson, Neils B. Quashie, Neils B. Quashie, Nancy O. Duah-Quashie

“…PF3D7_1441800 has been reported to reduce EVs production when knocked out in P. falciparum. Altogether, our findings suggest a role for EVs in developing ART resistance and warrant further studies interrogating this association.…”
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The mechanism of m6Am-modifying enzyme PCIF1 regulating target gene ACOT8 in gastric cancer progression by PENG Jin, WANG Weining, TAN Zhi, YE Guannan, ZHOU Zhen

“…Furthermore, ACOT8 expression was knocked down in PCIF1-overexpressing AGS cells, and rescue experiments were performed. …”
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Col1a1 mediates the focal adhesion pathway affecting hearing in miR-29a mouse model by RNA-seq analysis by Shuli Wang, Mulan Li, Pengcheng Liu, Yaning Dong, Ruishuang Geng, Tihua Zheng, Qingyin Zheng, Bo Li, Peng Ma

“…Pik3r3 and Ptk2 were differentially expressed in the basilar membranes and stria vascularis, but not at the s spiral ganglion neurons compared to miR-29a+/+ mice.Our results show that when miR-29a is knocked out, the Col1a1 mediates the focal adhesion pathway may affect the hearing of miR-29a−/− mice. …”
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Helicobacter pylori-induced aberrant demethylation and expression of GNB4 promotes gastric carcinogenesis via the Hippo–YAP1 pathway by Duanrui Liu, Yunyun Liu, Wenshuai Zhu, Yi Lu, Jingyu Zhu, Xiaoli Ma, Yuanxin Xing, Mingjie Yuan, Bin Ning, Yunshan Wang, Yanfei Jia

“…GNB4, TET1, and YAP1 were overexpressed or knocked down in GC cell lines. We performed gain- and loss-of-function experiments, including CCK-8, EdU, colony formation, transwell migration, and invasion assays. …”
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PI3 kinases p110α and PI3K-C2β negatively regulate cAMP via PDE3/8 to control insulin secretion in mouse and human islets by Jelena Kolic, Jocelyn E. Manning Fox, Oleg G. Chepurny, Aliya F. Spigelman, Mourad Ferdaoussi, Frank Schwede, George G. Holz, Patrick E. MacDonald

“…The expression of PI3K-C2β was knocked down using siRNA. We measured insulin release, single-cell exocytosis, intracellular Ca2+ responses ([Ca2+]i) and Ca2+ channel currents, intracellular cAMP concentrations ([cAMP]i), and activation of cAMP-dependent protein kinase A (PKA) and protein kinase B (PKB/AKT). …”
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Glucosinolate Profile and Glucosinolate Biosynthesis and Breakdown Gene Expression Manifested by Black Rot Disease Infection in Cabbage by Mehede Hassan Rubel, Md. Abuyusuf, Ujjal Kumar Nath, Arif Hasan Khan Robin, Hee Jeong Jung, Hoy Taek Kim, Jong In Park, Ill Sup Nou

“…This study helps in understanding the role of GSL biosynthesis and breakdown related genes for resistance against black rot pathogen in cabbage, which could be further confirmed through functional characterization either by overexpression or knock-out mutation.…”
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Bioinformatics analysis identifies coagulation factor II receptor as a potential biomarker in stomach adenocarcinoma by Xingwei Wu, Shengnan Wang, Chenci Wang, Chengwei Wu, Zhiyong Zhao

“…Drug sensitivity analysis demonstrated positive correlations between F2R and several drugs, including BEZ235, CGP-60474, Dasatinib, HG-6-64-1, Aazopanib, Rapamycin, Sunitinib and TGX221, while negative correlation with CP724714, FH535, GSK1904529A, JNK-9L, LY317615, pyrimidine, rTRAIL and Vinorelbine. Knocking down F2R in GC cell lines resulted in slowed proliferation, migration, and invasion. …”
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Histone Deacetylase 3 Regulates Adipocyte Phenotype at Early Stages of Differentiation by Dalma Cricrí, Lara Coppi, Silvia Pedretti, Nico Mitro, Donatella Caruso, Emma De Fabiani, Maurizio Crestani

“…HDAC3 was silenced in C3H/10T1/2 MSCs at different stages of differentiation to adipocytes. shRNA targeting HDAC3 was used to generate the knock-down model. Proper HDAC3 silencing was assessed by measuring both mRNA and protein levels of mouse HDAC3 via qPCR and western blot, respectively. …”
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