Climate-ready Landscape Plants: Garden Roses Trialed at Reduced Irrigation Frequency in Utah, USA by Ji-Jhong Chen, Youping Sun, Lorence R. Oki, Jared A. Sisneroz, Karrie Reid, Lloyd L. Nackley, Ryan N. Contreras, Soo-Hyung Kim, Ursula K. Schuch, Darren L. Haver, Miro Stuke, Allison Fron, Kelly Kopp, Scott B. Jones, Lawrence Hipps

“…The objective of this study was to determine the impact of reduced irrigation frequency on visual quality, plant growth, and physiology of five garden rose cultivars, including ChewPatout (Oso Easy® Urban Legend®), Meibenbino (Petite Knock Out®), MEIRIFTDAY (Oso Easy® Double Pink), Overedclimb (Cherry Frost™), and Radbeauty (Sitting Pretty™). …”
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Enhancement of the neutralization ability resulting from a single amino acid change in the light chain of a chimeric antibody against SARS-CoV-2 by YIN Zi, LIAN Chaoyang, GAO Bo, TIAN Ying, HAO Qian, YEAP Lengsiew

“…RNA was extracted to construct a mouse-origin antibody light chain gene library, which was paired with the knocked-in human-origin antibody heavy chain gene for antibody expression. …”
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USP1-Associated Factor 1 Modulates Japanese Encephalitis Virus Replication by Governing Autophagy and Interferon-Stimulated Genes by Jinchao Xing, Chen Hu, Siqi Che, Yixin Lan, Lihong Huang, Lele Liu, Youqin Yin, Huanan Li, Ming Liao, Wenbao Qi

“…To explore the possible roles of autophagy in UAF1-mediated inhibition of JEV propagation, we knocked out ATG7 to generate autophagy-deficient cells and found that depletion of UAF1 failed to promote JEV replication in ATG7 knockout cells. …”
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Pan‐cancer analyses of bromodomain containing 9 as a novel therapeutic target reveals its diagnostic, prognostic potential and biological mechanism in human tumours by Yu Chen, Zitong Gao, Isam Mohd‐Ibrahim, Hua Yang, Lang Wu, Yuanyuan Fu, Youping Deng

“…For experimental validation, high expression levels of BRD9 were observed in tumour tissue samples from both lung and colon cancer patients. Knocking down BRD9 led to the inhibition of lung and colon cancer development, likely via the Wnt/β‐catenin signalling pathway. …”
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Epstein-Barr Virus Promotes Tumor Angiogenesis by Activating STIM1-Dependent Ca²⁺ Signaling in Nasopharyngeal Carcinoma by Jiaxiang Ye, Jiazhang Wei, Yue Luo, Yayan Deng, Ting Que, Xiaojian Zhang, Fei Liu, Jinyan Zhang, Xiaoling Luo

“…STIM1 expression was detected in NPC cell lines HK1 and CNE2 that were negative or positive for EBV. STIM1 was knocked down in EBV-positive cells using recombinant lentivirus, then cytosolic Ca²⁺ levels were measured based on fluorescence resonance energy transfer. …”
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Hepatocyte-specific HDAC3 ablation promotes hepatocellular carcinoma in females by suppressing Foxa1/2 by Yahong Xu, Yongjie Zhu, Zhenru Wu, Shengfu Li, Mingyang Shao, Qing Tao, Qing Xu, Yuwei Chen, Yuke Shu, Menglin Chen, Yongjie Zhou, Yujun Shi

“…To further elucidate that HDAC3 controls the expression of Foxa1/2, we knocked down HDAC3 in HUH7 liver cancer cells. Results We observed a contrary sex disparity, with an earlier onset and higher incidence of HCC in female mice when HDAC3 was selectively ablated in the liver. …”
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The Mechanism of Alisol B23 Acetate Inhibiting Lung Cancer: Targeted Regulation of CD11b/CD18 to Influence Macrophage Polarization by Chen Y, Lu J, Xie Z, Tang J, Lian X, Li X

“…Finally, the expression of CD18 in A549 cells was knocked down to construct NSCLC tumor-bearing mice and AB23A was applied for intragastric administration. …”
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Targeting mTOR Complex 2 in Castration-Resistant Prostate Cancer with Acquired Docetaxel Resistance by Huang Y, Zhai Y, Wu M, Chang C, Luo J, Hong D, Zhao Q, Dai Y, Liu J

“…The lentivirus was transfected into cells to knock down the expression of Rictor, and cell viability was measured by Cell Counting Kit 8 (CCK-8). …”
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Gamma-aminobutyric acid type A receptor alpha 4 coordinates autophagy, inflammation, and immunometabolism to promote innate immune activation by Jin Kyung Kim, Prashanta Silwal, Young Jae Kim, Sang Min Jeon, In Soo Kim, June-Young Lee, Jun Young Heo, Sang-Hee Lee, Jin-Woo Bae, Jin-Man Kim, Jin Bong Park, Eun-Kyeong Jo

“…Abbreviation AM: Alveolar macrophage; AMPK: AMP-activated protein kinase; ASC: Apoptosis-associated speck-like protein containing a CARD; ATP: Adenosine 5’-triphosphate; BAL: Bronchoalveolar lavage; BCG: Mycobacterium bovis Bacillus Calmette–Guérin; BMDM: Bone marrow-derived macrophage; CCL: CC motif chemokine ligand; CFU: Colony forming unit; CKO: Conditional knock out; CXCL: C-X-C motif ligand; Dpi: Days post-infection; ECAR: Extracellular acidification rate; EGFP: Enhanced green fluorescent protein; FOXO3: Forkhead box O3; GABA: Gamma-aminobutyric acid; GABAAR: GABA type A receptor; Gabarap: GABA type A receptor-associated protein; Gabarapl1: GABA type A receptor-associated protein like 1; GABRA4: Gamma-aminobutyric acid type A receptor subunit alpha4; HIF-1α: Hypoxia-inducible factor-1 alpha; IL: Interleukin; i.n.: Intranasal; i.p.: Intraperitoneal; LDHA: Lactate dehydrogenase A; LPS: Lipopolysaccharide; Mabc: Mycobacteroides abscessus subsp. abscessus; MOI: Multiplicities of infection; Mtb: Mycobacterium tuberculosis; mtROS: Mitochondrial reactive oxygen species; OCR: Oxygen consumption rate; OXPHOS: Oxidative phosphorylation; PM: Peritoneal macrophage; TNF: Tumor necrosis factor; WT: Wild type…”
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IKKβ deletion from CNS macrophages increases neuronal excitability and accelerates the onset of EAE, while from peripheral macrophages reduces disease severity by Maria Avloniti, Maria Evangelidou, Maria Gomini, Theodore Loupis, Mary Emmanouil, Adamantia Mitropoulou, Theodore Tselios, Hans Lassmann, Agnès Gruart, José M. Delgado-García, Lesley Probert, Vasiliki Kyrargyri

“…Results Global depletion of IKKβ from myeloid cells in MφIKKβΚΟ mice accelerated the onset and significantly supressed chronic EAE. Knocking out IKKβ only from CNS resident macrophages accelerated the onset and exacerbated chronic EAE, accompanied by earlier demyelination and immune cell infiltration but had no effect in cuprizone-induced demyelination. …”
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Interleukin4Rα (IL4Rα) and IL13Rα1 Are Associated with the Progress of Renal Cell Carcinoma through Janus Kinase 2 (JAK2)/Forkhead Box O3 (FOXO3) Pathways by Mi-Ae Kang, Jongsung Lee, Sang Hoon Ha, Chang Min Lee, Kyoung Min Kim, Kyu Yun Jang, See-Hyoung Park

“…For the in vitro evaluation of the oncogenic role of IL4Rα and IL13Rα1 in RCC, we knock-downed IL4Rα or IL13Rα1 and observed that the cell proliferation rate was decreased, and the apoptosis rate was increased in A498 and ACHN cells. …”
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Low-Salt Diet Reduces Anti-CTLA4 Mediated Systemic Immune-Related Adverse Events while Retaining Therapeutic Efficacy against Breast Cancer by Durga Khandekar, Debolanle O. Dahunsi, Isaac V. Manzanera Esteve, Sonya Reid, Jeffrey C. Rathmell, Jens Titze, Venkataswarup Tiriveedhi

“…Importantly, CD4-specific knock out of osmosensitive transcription factor NFAT5 using CD4cre/creNFAT5flox/flox transgenic mice caused a downregulation of high-salt-mediated inflammatory activation of CD4+T cells and irAE response. …”
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BET inhibitor JQ1 suppresses cell proliferation via inducing autophagy and activating LKB1/AMPK in bladder cancer cells by Feng Li, Chao Yang, Hai‐Bao Zhang, Jianbin Ma, Jing Jia, Xiaoshuang Tang, Jin Zeng, Tie Chong, Xinyang Wang, Dalin He, Peng Guo

“…AMPK siRNA was used to knock down AMPK. T24 xenograft model in mice was chosen to perform in vivo studies. …”
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miR-29b-3p regulates cardiomyocytes pyroptosis in CVB3-induced myocarditis through targeting DNMT3A by Ya Wang, Zhengyang Zhang, Hui Li, Min Wang, Yuting Qiu, Lili Lu

“…To explore the function of miR-29b-3p in CVB3-induced VMC, we conducted in vivo experiments by knocking down the expression of miR-29b-3p using antagomir. …”
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Cuproptosis‐related molecular patterns and gene (ATP7A) in hepatocellular carcinoma and their relationships with tumor immune microenvironment and clinical features by Shanbao Li, Junyong Weng, Chao Xiao, Jing Lu, Wanyue Cao, Fangbin Song, Zeping He, Peng Zhang, Zhonglin Zhu, Junming Xu

“…The ATP7A had the highest hazard ratio (HR = 1.465, p < .001), was high expression in HCC tissues and with a shorter 5‐year OS. Knocking down ATP7A could enhance intracellular copper concentration, cause a decrease in DLAT expression, and induce cuproptosis and inhibit cell proliferation and migration. …”
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Mitochondrial damage and activation of the cytosolic DNA sensor cGAS–STING pathway lead to cardiac pyroptosis and hypertrophy in diabetic cardiomyopathy mice by Meiling Yan, Yun Li, Qingmao Luo, Wenru Zeng, Xiaoqi Shao, Lun Li, Qing Wang, Dongwei Wang, Yue Zhang, Hongtao Diao, Xianglu Rong, Yunlong Bai, Jiao Guo

“…C57BL/6J mice were preinjected with adeno-associated virus 9 (AAV9) intravenously via the tail vein to specifically knock down myocardial STING. After four weeks, mice with myocardium-specific knockdown of STING received injections of streptozotocin (STZ; 50 mg/kg) and a high-fat diet to induce diabetes. …”
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Identification and characterization of CLEC11A and its derived immune signature in gastric cancer by Qing Zheng, Qing Zheng, Zhenqi Gong, Zhenqi Gong, Baizhi Li, Baizhi Li, Runzi Cheng, Runzi Cheng, Weican Luo, Cong Huang, Huaiming Wang

“…Moreover, the flow cytometry assay showed that the knock-down of CLEC11A increased the infiltration of cytotoxic CD8+ T cells and helper CD4+ T into tumors while decreasing the percentage of M2 macrophages, MDSCs, and Tregs.ConclusionCollectively, our findings revealed that CLEC11A could be a prognostic and immunological biomarker in GC, and CLEC11A-derived immune signature might serve as a new option for clinicians to predict outcomes and formulate personalized treatment plans for GC patients.…”
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Effect of low expression of senescence marker protein 30 on proliferation and oxidation of human lens epithelial cells line SRA01/04 under high calcium conditions by Song-Man Li, Zi-Hao Han, Aint Thu Thu Win, Xi Chen, Hao Liang

“…<p>METHODS: Three RNAi sequences were designed to knock down SMP30 target gene RGN expression(KD1-3), and the blank-load sequence was used as the negative control group(NCKD), all of which were used to construct lentiviral vectors to infect SRA01/04 cells. …”
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Protective Effect of EGCG on Hydrogen Peroxide-induced Apoptosis of Human Cardiomyocyte via Regulating miR-16-5p/AOC1 Axis by Yingxin BI, Xianjun LIU, Xianglong MENG, Yue LIU, Mengyuan WANG, Yan ZHANG, Zhandong LI, Yuhe YIN, Hao LI

“…In apoptotic cells, the expression of AOC1 was significantly decreased, and EGCG could significantly up-regulate the expression of AOC1, while knocking down AOC1 could significantly reverse the inhibition of EGCG on apoptosis (P<0.05). …”
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OLFM2 promotes epithelial-mesenchymal transition, migration, and invasion in colorectal cancer through the TGF-β/Smad signaling pathway by Yong Tang, Yi Liu, Xiaobo Wang, Haiyang Guo, Lin Chen, Guangbing Hu, Yutong Cui, Shiqi Liang, Ji Zuo, Zichen Luo, Xinrui Chen, Xianfei Wang

“…To investigate the function of OLFM2, we overexpressed and knocked down OLFM2. We discovered that OLFM2 knockdown inhibited migration and invasion of colon cancer cells. …”
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