Regulation of gene expression during DNA damage response in Plasmodium falciparum by Gupta, Devendra Kumar

“…Using endogenous tagging and knock-out approach, our results suggest that PF14_0271 is a schizont specific transcriptional regulator.…”
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Evaluation of the role of miR-31-dependent reduction in dystrophin and nNOS on atrial-fibrillation-induced electrical remodelling in man by Reilly, S, Liu, X, Carnicer, R, Rajakumar, T, Sayeed, R, Krasopoulos, G, Verheule, S, Fulga, T, Schotten, U, Casadei, B

“…We also used a goat model of pacing-induced atrial fibrillation (24 with atrial fibrillation vs 20 controls in normal sinus rythm) and nNos-knock-out mice (n=28 compared with 27 wild-type littermates). …”

RNA and glia at the Drosophila neuromuscular junction: importance for synaptic plasticity by Gala, DS

“…In Chapter 4, I evaluated the effects of knocking down these 18 mRNAs in glia using an RNA interference (RNAi) candidate screen. …”

The new pathology: dissecting cellular responses in lupus nephritis by Bhandari, A

“…To test the role of ITGA8 in mesangial cells, <em>ITGA8</em> was knocked down in vitro, resulting in a more fibroblast like phenotype, akin to that seen in the kidneys of imiquimod treated mice. …”

Characterisation of a novel mouse model of mitochondrial disease – a hypomorphic Wars2 ENU-induced allele by Agnew, T

“…Studying mtRS mutations in model organisms is difficult because mtRS knock-out alleles are haplosufficient and cause embryonic lethality in their homozygous state. …”

Functional properties of the intact and compromised midbrain dopamine system by Kaufmann, A

“…Similar effects on firing variability and burst firing of SNc dopamine neurons were found in a mouse model with conditional knock- out of the transcription factors Forkhead box A1 and A2 (FoxA1/2) in midbrain dopamine neurons. …”

The effect of manipulating the expression of the NR2B subunit of the NMDA receptor on learning and memory by Hoon, A

“…</p><p>We also considered the effects of knocking out the NR2B subunit on learning and memory, and NR1 subunit deletion within the hippocampus. …”

Selective targeting of GARP-LTGFβ axis in the tumor microenvironment augments PD-1 blockade via enhancing CD8+ T cell antitumor immunity by Yi Wang, David Carbone, Zihai Li, Margaret Gatti-Mays, Yiping Yang, Kai He, Anqi Li, Qin Ma, Yuzhou Chang, No-Joon Song, Xingjun Wu, Dongjun Chung, Brian P Riesenberg, Maria Velegraki, Giuseppe D Giuliani, Komal Das, Tamio Okimoto, Hyunwoo Kwon, Karthik B Chakravarthy, Chelsea Bolyard, Jayajit Das, Daniel T Gewirth

“…Using human LRRC32 (encoding GARP) knock-in mice, we find that PIIO-1 does not cause thrombocytopenia; is preferentially distributed in the TME; and exhibits therapeutic efficacy against GARP+ and GARP- cancers, alone or in combination with anti-PD-1 antibody. …”
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Loss of Protein Function Causing Severe Phenotypes of Female-Restricted Wieacker Wolff Syndrome due to a Novel Nonsense Mutation in the <i>ZC4H2</i> Gene by Jing-Jing Sun, Qin Cai, Miao Xu, Yan-Na Liu, Wan-Rui Li, Juan Li, Li Ma, Cheng Cai, Xiao-Hui Gong, Yi-Tao Zeng, Zhao-Rui Ren, Fanyi Zeng

“…Subsequently, expression levels of <i>ZC4H2</i> were knocked down in neural stem cells (NSCs) derived from induced pluripotent stem cells (iPSCs) by lentiviral-expressed small hairpin RNAs (shRNAs) against <i>ZC4H2</i>. …”
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A new Hoxb8FlpO mouse line for intersectional approaches to dissect developmentally defined adult sensorimotor circuits by Manon Bohic, Manon Bohic, Aman Upadhyay, Aman Upadhyay, Aman Upadhyay, Jaclyn T. Eisdorfer, Jaclyn T. Eisdorfer, Jessica Keating, Jessica Keating, Jessica Keating, Jessica Keating, Rhiana C. Simon, Brandy A. Briones, Chloe Azadegan, Chloe Azadegan, Hannah D. Nacht, Hannah D. Nacht, Olisemeka Oputa, Olisemeka Oputa, Alana M. Martinez, Alana M. Martinez, Bridget N. Bethell, Mark A. Gradwell, Mark A. Gradwell, Peter Romanienko, Matt S. Ramer, Garret D. Stuber, Victoria E. Abraira, Victoria E. Abraira

“…We show how developmental knock-out versus transient adult silencing of the same ROR𝛃 lineage neurons affects adult sensorimotor behavior. …”
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Low-intensity pulsed ultrasound triggers a beneficial neuromodulation in dementia mice with chronic cerebral hypoperfusion via activation of hippocampal Fndc5/irisin signaling by Degang Song, Xiaomin Chen, Na Zhou, Yi Yuan, Shuo Geng, Cong Zhang, Zhe Zhao, Xingran Wang, Xinran Bao, Xifa Lan, Xiangjian Zhang

“…The levels of Fndc5/irisin in different brain regions, the hippocampal long-term potentiation and anti-inflammatory cytokines were investigated at day 28 after cognitive evaluation. Global Fndc5 knock-out (F5KO), forced expression or knockdown of Fndc5, and recombinant irisin application were respectively employed for mechanism exploration. …”
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Peroxisomal Proliferator-Activated Receptor β/δ Deficiency Induces Cognitive Alterations by Triana Espinosa-Jiménez, Triana Espinosa-Jiménez, Triana Espinosa-Jiménez, Oriol Busquets, Amanda Cano, Amanda Cano, Amanda Cano, Amanda Cano, Elena Sánchez-López, Elena Sánchez-López, Elena Sánchez-López, Elena Sánchez-López, Ester Verdaguer, Ester Verdaguer, Ester Verdaguer, Antoni Parcerisas, Jordi Olloquequi, Carme Auladell, Carme Auladell, Carme Auladell, Jaume Folch, Jaume Folch, Walter Wahli, Walter Wahli, Walter Wahli, Manuel Vázquez-Carrera, Manuel Vázquez-Carrera, Manuel Vázquez-Carrera, Antoni Camins, Antoni Camins, Antoni Camins, Miren Ettcheto, Miren Ettcheto, Miren Ettcheto

“…To carry out this study, 6-month-old total knock-out for the Ppard gene male mice with C57BL/6X129/SV background (PPARβ/δ-/-) and wild-type (WT) littermates with the same genetic background were used. …”
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Transforming growth factor receptor III (Betaglycan) regulates the generation of pathogenic Th17 cells in EAE by Samuel J. Duesman, Sandra Ortega-Francisco, Sandra Ortega-Francisco, Roxana Olguin-Alor, Naray A. Acevedo-Dominguez, Christine M. Sestero, Rajeshwari Chellappan, Patrizia De Sarno, Nabiha Yusuf, Adrian Salgado-Lopez, Marisol Segundo-Liberato, Marisol Segundo-Liberato, Selina Montes de Oca-Lagunas, Chander Raman, Gloria Soldevila, Gloria Soldevila

“…To begin addressing this question, we developed a conditional knock-out mouse with restricted TβRIII deletion in mature T cells, necessary because genomic deletion of TβRIII results in perinatal mortality. …”
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miR-281, an abundant midgut-specific miRNA of the vector mosquito Aedes albopictus enhances dengue virus replication by Yanhe Zhou, Yanxia Liu, Hui Yan, Yiji Li, Hao Zhang, Jiabao Xu, Santhosh Puthiyakunnon, Xiaoguang Chen

“…Methods The expression profiles of miR-281 in different body parts of Ae. albopictus and following dengue virus infection were determined using RT-qPCR and Northern blot. miR-281 mimics, antagomiRs and corresponding negative controls were designed and their overexpression and knock-down efficiency were analyzed by qRT-PCR after transfecting the mosquito cell lines C6/36, and also by injecting female mosquitoes. …”
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MiR-7-5p/KLF4 signaling inhibits stemness and radioresistance in colorectal cancer by Yuanyuan Shang, Zhe Zhu, Yuanyuan Zhang, Fang Ji, Lian Zhu, Mengcheng Liu, Yewei Deng, Guifen Lv, Dan Li, Zhuqing Zhou, Bing Lu, Chuan-gang Fu

“…Overexpression of miR-7-5p led to a rescue of radioresistance and an increase in radiation-induced apoptosis, and attenuated the stem cell-like properties in HCT116-R and RKO-R cells. Conversely, knocking down miR-7-5p in parental HCT116 and RKO cells suppressed the sensitivity to radiation treatment and enhance cancer cell stemness. …”
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Differential Effect of Dietary Supplementation with a Soybean Oil Enriched in Oleic Acid versus Linoleic Acid on Plasma Lipids and Atherosclerosis in LDLR-Deficient Mice by Zhi-Hong Yang, Kimball Nill, Yuki Takechi-Haraya, Martin P. Playford, David Nguyen, Zu-Xi Yu, Milton Pryor, Jingrong Tang, Krishna Vamsi Rojulpote, Nehal N. Mehta, Han Wen, Alan T. Remaley

“…To test whether diets rich in CSO versus MSO would attenuate atherosclerosis development, LDL receptor knock-out (LDLR-KO) mice were fed a Western diet enriched in saturated fatty acids (control), or a Western diet supplemented with 5% (<i>w</i>/<i>w</i>) LA-rich CSO or high-oleic MSO for 12 weeks. …”
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Heparanase inhibition as a systemic approach to protect the endothelial glycocalyx and prevent microvascular complications in diabetes by Monica Gamez, Hesham E. Elhegni, Sarah Fawaz, Kwan Ho Ho, Neill W. Campbell, David A. Copland, Karen L. Onions, Matthew J. Butler, Elizabeth J. Wasson, Michael Crompton, Raina D. Ramnath, Yan Qiu, Yu Yamaguchi, Kenton P. Arkill, David O. Bates, Jeremy E. Turnbull, Olga V. Zubkova, Gavin I. Welsh, Denize Atan, Simon C. Satchell, Rebecca R. Foster

“…Methods In this study, two mouse models of heparan sulphate depletion (enzymatic removal and genetic ablation by endothelial specific Exotosin-1 knock down) were utilized to investigate the impact of endothelial cell surface (i.e., endothelial glycocalyx) heparan sulphate loss on microvascular barrier function. …”
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m6A methyltransferase METTL3 programs CD4+ T-cell activation and effector T-cell differentiation in systemic lupus erythematosus by Shuang Lu, Xingyu Wei, Huan Zhu, Zhi Hu, Meiling Zheng, Jiali Wu, Cheng Zhao, Shuang Yang, Delong Feng, Sujie Jia, Hongjun Zhao, Ming Zhao

“…Methods The expression of METTL3 was knocked down and METTL3 enzyme activity was inhibited using siRNA and catalytic inhibitor, respectively. …”
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TRPM4 Participates in Aldosterone-Salt-Induced Electrical Atrial Remodeling in Mice by Christophe Simard, Virginie Ferchaud, Laurent Sallé, Paul Milliez, Alain Manrique, Joachim Alexandre, Romain Guinamard

“…The experiments were conducted in wild type animals (<i>Trpm4^+/+</i>) as well as <i>Trpm4</i> knock-out animals (<i>Trpm4^-/-</i>). The atrial diameter measured by echocardiography was higher in <i>Trpm4^-/-</i> compared to <i>Trpm4^+/+</i> animals, and hyperaldosteronemia-salt produced a dilatation in both groups. …”
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ZIP11 Regulates Nuclear Zinc Homeostasis in HeLa Cells and Is Required for Proliferation and Establishment of the Carcinogenic Phenotype by Monserrat Olea-Flores, Monserrat Olea-Flores, Julia Kan, Alyssa Carlson, Sabriya A. Syed, Cat McCann, Varsha Mondal, Cecily Szady, Heather M. Ricker, Amy McQueen, Juan G. Navea, Leslie A. Caromile, Teresita Padilla-Benavides

“…To test this, we utilized HeLa cells, as research in humans correlated elevated expression of ZIP11 with poor prognosis in cervical cancer patients. We stably knocked down ZIP11 in HeLa cancer cells and investigated the effect of Zn dysregulation in vitro. …”
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