Based on different immune responses under the glucose metabolizing type of papillary thyroid cancer and the response to anti-PD-1 therapy by Wenjun Xie, Wenjun Xie, Wenjun Xie, Yu Zeng, Linfei Hu, Jiaru Hao, Yuzheng Chen, Yuzheng Chen, Xinwei Yun, Qiang Lin, Qiang Lin, Huashui Li, Huashui Li

“…To verify the accuracy of the prognostic prediction model, we knocked down PGBD5 in PTC cells and found that the proliferation ability of PTC cells was significantly reduced. …”
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Poly(I:C)-exposed zebrafish shows autism-like behaviors which are ameliorated by fabp2 gene knockout by Jing Wu, Xueting Lin, Dian Wu, Binhong Yan, Mengyi Bao, Peilei Zheng, Jiangping Wang, Cuiwei Yang, Zhongxia Li, Xiaoming Jin, Xiaoming Jin, Kewen Jiang, Kewen Jiang

“…Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein–protein interaction (PPI) network analysis was performed with the detected DEGs to find the concentrated pathways. Finally, we knocked out the fatty acid-binding protein 2 (fabp2), a key node of the concentrated PPI network, to find its rescues on the altered social behavior.ResultsWe reported here that MIA offspring born to mothers injected with poly(I:C) exhibited impaired social approach and social cohesion that mimicked human ASD phenotypes. …”
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Fine Mapping and Functional Research of Key Genes for Photoperiod Sensitivity in Maize by Jianbo Fei, Jianbo Fei, Qingping Jiang, Qingping Jiang, Mingyang Guo, Mingyang Guo, Jianyu Lu, Jianyu Lu, Piwu Wang, Piwu Wang, Siyan Liu, Siyan Liu, Jing Qu, Jing Qu, Yiyong Ma, Yiyong Ma, Shuyan Guan, Shuyan Guan

“…We also found that the phenotypes of the positive plants were significantly different from the control plants when they overexpressed the objective gene or when it was knocked out, and the expression period, phase, and amplitude of the target gene also shifted. …”
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Metformin increases the uptake of glucose into the gut from the circulation in high-fat diet-fed male mice, which is enhanced by a reduction in whole-body Slc2a2 expression by Nicola Morrice, Susanne Vainio, Kirsi Mikkola, Lidy van Aalten, Jennifer R. Gallagher, Michael L.J. Ashford, Alison D. McNeilly, Rory J. McCrimmon, Alexandra Grosfeld, Patricia Serradas, Jukka Koffert, Ewan R. Pearson, Pirjo Nuutila, Calum Sutherland

“…However, there was no effect of specifically knocking-out Slc2a2 in the mouse intestinal epithelial cells. …”
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O-GlcNAc regulates the mitochondrial integrated stress response by regulating ATF4 by Ibtihal M. Alghusen, Marisa S. Carman, Heather Wilkins, Heather Wilkins, Heather Wilkins, Sophiya John Ephrame, Amy Qiang, Wagner B. Dias, Halyna Fedosyuk, Aspin R. Denson, Russell H. Swerdlow, Russell H. Swerdlow, Russell H. Swerdlow, Chad Slawson, Chad Slawson

“…An indirect downstream target of ATF4 mitochondrial chaperone glucose-regulated protein 75 (GRP75) is significantly elevated. Interestingly, knock-down of O-GlcNAc transferase (OGT), the enzyme that adds O-GlcNAc, in SH-SY5Y increases ATF4 protein and mRNA expression. …”
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Spermidine/Spermine N1-Acetyltransferase 1 (<i>SAT1</i>)—A Potential Gene Target for Selective Sensitization of Glioblastoma Cells Using an Ionizable Lipid Nanoparticle to Deliver... by Vinith Yathindranath, Nura Safa, Babu V. Sajesh, Kelly Schwinghamer, Magimairajan Issai Vanan, Rashid Bux, Daniel S. Sitar, Marshall Pitz, Teruna J. Siahaan, Donald W. Miller

“…The LNP-si<i>SAT1</i> effectively knocked down the <i>SAT1</i> expression in U251, LN229, and 42MGBA GB cells, and other brain-relevant endothelial (hCMEC/D3), astrocyte (HA) and macrophage (ANA-1) cells at the mRNA and protein levels. …”
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<i>NCAPG</i> Regulates Myogenesis in Sheep, and SNPs Located in Its Putative Promoter Region Are Associated with Growth and Development Traits by Zehu Yuan, Ling Ge, Pengwei Su, Yifei Gu, Weihao Chen, Xiukai Cao, Shanhe Wang, Xiaoyang Lv, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Wei Sun

“…The function of <i>NCAPG</i> in myoblast proliferation and differentiation was detected after small interfering RNAs (siRNAs) knocked down the expression of <i>NCAPG</i>. Cell proliferation was detected using CCK-8 assay, EdU proliferation assay, and flow cytometry cell cycle analysis. …”
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CD44 Contributes to the Regulation of MDR1 Protein and Doxorubicin Chemoresistance in Osteosarcoma by Monserrat Gerardo-Ramírez, Friederike L. Keggenhoff, Vanessa Giam, Diana Becker, Marco Groth, Nils Hartmann, Beate K. Straub, Helen Morrison, Peter R. Galle, Jens U. Marquardt, Peter Herrlich, Monika Hartmann

“…Here, we investigated the role of CD44 in the regulation of drug chemoresistance, using osteosarcoma cells isolated from mice carrying a mutation of the tumor suppressor neurofibromatosis type 2 (<i>Nf2</i>) gene. CD44 expression was knocked-down in the cells using CRISPR/Cas9 approach. …”
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Nogo-A Is Critical for Pro-Inflammatory Gene Regulation in Myocytes and Macrophages by H. M. Arif Ullah, A. K. Elfadl, SunYoung Park, Yong Deuk Kim, Myung-Jin Chung, Ji-Yoon Son, Hyun-Ho Yun, Jae-Min Park, Jae-Hyuk Yim, Seung-Jun Jung, Young-Chul Choi, Jin-Hong Shin, Dae-Seong Kim, Jin-Kyu Park, Kyu-Shik Jeong

“…We therefore hypothesized that Nogo-A might relate to skeletal muscle diseases. According to our knocking down and overexpression results in muscle cell line (C2C12), we have found that upregulation of Nogo-A resulted in upregulation of CHOP, pro-inflammatory cytokines such as interleukin (IL)-6 and tumor necrosis factor (TNF)-α, while downregulation of Nogo-A led to downregulation of CHOP, IL-6 and TNF-α. …”
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Targeting the SREBP-1/Hsa-Mir-497/SCAP/FASN Oncometabolic Axis Inhibits the Cancer Stem-like and Chemoresistant Phenotype of Non-Small Cell Lung Carcinoma Cells by Tung-Yu Tiong, Pei-Wei Weng, Chun-Hua Wang, Syahru Agung Setiawan, Vijesh Kumar Yadav, Narpati Wesa Pikatan, Iat-Hang Fong, Chi-Tai Yeh, Chia-Hung Hsu, Kuang-Tai Kuo

“…Methods: We analyzed SREBP-1 levels and biological functions in clinical samples and the human NSCLC cell lines H441 and A549 through shRNA-based knock down of SREBP function, cisplatin-resistant clone generation, immunohistochemical staining of clinical samples, and cell viability, sphere-formation, Western blot, and quantitative PCR assays. …”
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A Potential Mechanism of Tumor Progression during Systemic Infections Via the Hepatocyte Growth Factor (HGF)/c-Met Signaling Pathway by Hironori Tsujimoto, Hiroyuki Horiguchi, Yusuke Matsumoto, Risa Takahata, Nariyoshi Shinomiya, Takao Yamori, Hiromi Miyazaki, Satoshi Ono, Daizoh Saitoh, Yoji Kishi, Hideki Ueno

“…Intraperitoneal injection of mouse recombinant HGF significantly promoted liver metastases in sham-operated mice on 14 days after surgery. Knocking down c-Met expression on NL17 tumor cells by RNAi technology significantly inhibited the promotion of CLP-induced liver metastases. …”
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Age- and Sex-Associated Pathogenesis of Cell Culture-Passaged Kemerovo Virus in IFNAR^(−/−) Mice by Camille Victoire Migné, Aurélie Heckmann, Baptiste Monsion, Fauziah Mohd Jaafar, Clémence Galon, Sabine Rakotobe, Lesley Bell-Sakyi, Sara Moutailler, Houssam Attoui

“…Previous animal experimentation studies with orbiviruses, in particular the interferon receptor double knock-out (IFNAR^(−/−)) mouse model, did not indicate bias that is related to age or sex. …”
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Class A Penicillin-Binding Protein C Is Responsible for Stress Response by Regulation of Peptidoglycan Assembly in Clavibacter michiganensis by Xing Chen, Yao Li, Kaihong Bai, Meng Gu, Xiaoli Xu, Na Jiang, Yu Chen, Jianqiang Li, Laixin Luo

“…Here we systematically evaluated the stress response strategies in Gram-positive rod-shaped bacterium C. michiganensis log phase cells and revealed a significant increase of peptidoglycan thickness and slight increase of cross-linkage after Cu2+ treatment. Most strikingly, knocking-out of PBPC leads to a significant increase in cross-linking of peptidoglycan in response to Cu2+ treatment. …”
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MerTK Expression and ERK Activation Are Essential for the Functional Maturation of Osteopontin‐Producing Reparative Macrophages After Myocardial Infarction by Kohsuke Shirakawa, Jin Endo, Masaharu Kataoka, Yoshinori Katsumata, Atsushi Anzai, Hidenori Moriyama, Hiroki Kitakata, Takahiro Hiraide, Seien Ko, Shinichi Goto, Genki Ichihara, Keiichi Fukuda, Tohru Minamino, Motoaki Sano

“…In Il‐10 knockout enhanced green fluorescent protein–Spp1 knock‐in mice subjected to MI, osteopontin‐producing macrophages decreased but did not disappear entirely. …”
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Genome-Wide Transcriptional Analysis and Functional Validation Linked a Cluster of Epsilon Glutathione S-Transferases with Insecticide Resistance in the Major Malaria Vector <i>Ano... by Mersimine F. M. Kouamo, Sulaiman S. Ibrahim, Jack Hearn, Jacob M. Riveron, Michael Kusimo, Magellan Tchouakui, Terence Ebai, Williams Tchapga, Murielle J. Wondji, Helen Irving, Thaddée Boudjeko, Fabrice F. Boyom, Charles S. Wondji

“…Validation of <i>GSTe</i> genes expression profiles by qPCR confirmed that the genes are differentially expressed across Africa with a greater overexpression in DDT-resistant mosquitoes. RNAi-based knock-down analyses supported that five <i>GSTe</i> genes are playing a major role in resistance to pyrethroids (permethrin and deltamethrin) and DDT in <i>An. funestus</i>, with a significant recovery of susceptibility observed when <i>GSTe2</i>, <i>3</i>, <i>4</i>, <i>5</i> and <i>GSTe6</i> were silenced. …”
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Stroma-derived miR-214 coordinates tumor dissemination by Francesca Orso, Federico Virga, Daniela Dettori, Alberto Dalmasso, Mladen Paradzik, Aurora Savino, Margherita A. C. Pomatto, Lorena Quirico, Stefania Cucinelli, Martina Coco, Katia Mareschi, Franca Fagioli, Leonardo Salmena, Giovanni Camussi, Paolo Provero, Valeria Poli, Massimiliano Mazzone, Pier Paolo Pandolfi, Daniela Taverna

“…Methods We used public databases to investigate miR-214 expression in the stroma compartment of primary human samples and evaluated tumor formation and dissemination following tumor cell injections in miR-214 overexpressing (miR-214over) and knock out (miR-214ko) mice. In addition, we dissected the impact of Conditioned Medium (CM) or Extracellular Vesicles (EVs) derived from miR-214-rich or depleted stroma cells on cell metastatic traits. …”
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Interdependent Transcription of a Natural Sense/Antisense Transcripts Pair (<em>SLC34A1/PFN3</em>) by Hany S. Zinad, Chanachai Sae-Lee, Maria Ascensión Ariza-Mateos, Grace Adamson, Mushtaq Mufleh Khazeem, Amber Knox, Git Chung, Jelena Mann, Andreas Werner

“…Concordant expression is confirmed with the antisense knock-in that also enhances sense expression. The antisense effect acts on transcription in cis since transient transfection with sense or antisense constructs fails to stimulate the expression of the opposite transcript. …”
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IGF2 Mediates Resistance to Isoform-Selective-Inhibitors of the PI3K in HPV Positive Head and Neck Cancer by Mai Badarni, Manu Prasad, Artemiy Golden, Baisali Bhattacharya, Liron Levin, Ksenia M. Yegodayev, Orr Dimitstein, Ben-Zion Joshua, Limor Cohen, Ekaterina Khrameeva, Dexin Kong, Angel Porgador, Alex Braiman, Jennifer R. Grandis, Barak Rotblat, Moshe Elkabets

“…Stimulating tumor cells with recombinant IGF2 limited isiPI3K efficacy and released treated cells from S phase arrest. Knocking-down IGF2 with siRNA, or blocking IGF1R with AEW541, resulted in superior anti-tumor activity of isiPI3K in vitro and ex vivo. …”
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Histidine-rich glycoprotein in metabolic dysfunction-associated steatohepatitis-related disease progression and liver carcinogenesis by Beatrice Foglia, Salvatore Sutti, Stefania Cannito, Chiara Rosso, Marina Maggiora, Alice Casalino, Claudia Bocca, Erica Novo, Francesca Protopapa, Naresh Naik Ramavath, Alessia Provera, Alessandro Gambella, Elisabetta Bugianesi, Frank Tacke, Emanuele Albano, Maurizio Parola

“…Here we investigated the role of histidine-rich glycoprotein (HRG), a plasma protein produced by hepatocytes, in MASLD/MASH progression and HCC development.MethodsThe role of HRG was investigated by morphological, cellular, and molecular biology approaches in (a) HRG knock-out mice (HRG−/− mice) fed on a CDAA dietary protocol or a MASH related diethyl-nitrosamine/CDAA protocol of hepatocarcinogenesis, (b) THP1 monocytic cells treated with purified HRG, and (c) well-characterized cohorts of MASLD patients with or without HCC.ResultsIn non-neoplastic settings, murine and clinical data indicate that HRG increases significantly in parallel with disease progression. …”
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Visualization of basement membranes by a nidogen-based fluorescent reporter in mice by Sugiko Futaki, Ayano Horimoto, Chisei Shimono, Naoko Norioka, Yukimasa Taniguchi, Hitomi Hamaoka, Mari Kaneko, Mayo Shigeta, Takaya Abe, Kiyotoshi Sekiguchi, Yoichi Kondo

“…For in vivo BM imaging, a knock-in reporter mouse line expressing human nidogen-1 fused to the red fluorescent protein mCherry (R26-CAG-Nid1-mCherry) was generated. …”
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