Regulation of CD45 phosphatase by oncogenic ALK in anaplastic large cell lymphoma by Giulia Mura, Elif Karaca Atabay, Matteo Menotti, Cinzia Martinengo, Chiara Ambrogio, Chiara Ambrogio, Gloria Giacomello, Maddalena Arigoni, Maddalena Arigoni, Martina Olivero, Martina Olivero, Raffaele A. Calogero, Raffaele A. Calogero, Roberto Chiarle, Roberto Chiarle, Claudia Voena

“…Next, we found that knocking-out CD45 with the CRISPR/Cas9 system resulted in increased resistance to ALK TKI treatment and CD45 was down-regulated in ALCL cells that developed resistance in vitro to ALK TKIs. …”
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Prohibitin 1 interacts with p53 in the regulation of mitochondrial dynamics and chemoresistance in gynecologic cancers by Bao Kong, Chae Young Han, Se Ik Kim, David A. Patten, Youngjin Han, Euridice Carmona, Dar-Bin Shieh, Annie C. Cheung, Anne-Marie Mes-Masson, Mary-Ellen Harper, Yong Sang Song, Benjamin K. Tsang

“…Results We report that: (1) Knock-down of Phb1 prevents Cisplatin (cis-diamine-dichloroplatinum; CDDP) -induced changes in mitochondrial fragmentation and Oma1 mediated cleavage, and Opa1 processing; (2) In response to CDDP, Phb1 facilitates the p-p53 (ser15)-Phb1-Bak interaction in mitochondria in chemosensitive gynecologic cancer cells but not in chemoresistant cells; (3) Akt overexpression results in suppressed p-p53(Ser15)-Phb1 interaction and dysregulated mitochondrial dynamics, and (4) Consistent with in vitro findings, proximity ligation assessment (PLA) in human ovarian tumor sections demonstrated that p-p53(ser15)-Phb1-Bak interaction in mitochondria is associated with better chemoresponsiveness and clinical outcome of patients. …”
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Extracellular matrix remodelling and stiffening contributes to tumorigenesis of salivary carcinoma ex pleomorphic adenoma——A study based on patient-derived organoids by Wanling Chen, Ting Gu, Qianqian Chen, Chuxiang Qu, Chunye Zhang, Yuhua Hu, Ronghui Xia, Ying Zhang, Min Wang, Xinyi Huang, Jiang Li, Chaoji Shi, Zhen Tian

“…Moreover, surgical specimens demonstrated a higher TWIST1 expression in CXPA over PA. After knocking down TWIST1 in CXPA cells, cell proliferation, migration, and invasiveness were significantly inhibited (p < 0.01). …”
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Feasibility, acceptability, and short-term impact of a brief sexually transmitted infection intervention targeting U.S. Military personnel and family members by Anjali Kunz, Amber Moodley, Donn J. Colby, Michele Soltis, Wesley Robb-McGrath, Alexandra Fairchok, Paul Faestel, Amanda Jungels, Alexis A. Bender, Edwin Kamau, Gina Wingood, Ralph DiClemente, Paul Scott

“…Methods The KISS intervention (Knocking out Infections through Safer-sex and Screening) was adapted from an evidence-based intervention endorsed by the US Centers for Disease Control and Prevention and consisted of a one-time, small group session. …”
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Effective editing for lysophosphatidic acid acyltransferase 2/5 in allotetraploid rapeseed (Brassica napus L.) using CRISPR-Cas9 system by Kai Zhang, Liluo Nie, Qiqi Cheng, Yongtai Yin, Kang Chen, Fuyu Qi, Dashan Zou, Haohao Liu, Weiguo Zhao, Baoshan Wang, Maoteng Li

“…However, because B. napus is an allotetraploid with two subgenomes, the precise genes which involved in oil production remain unclear due to the intractability of efficiently knocking out all copies with high genetic redundancy. …”
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Prometastatic Effect of ATX Derived from Alveolar Type II Pneumocytes and B16-F10 Melanoma Cells by Mélanie A. Dacheux, Sue Chin Lee, Yoojin Shin, Derek D. Norman, Kuan-Hung Lin, Shuyu E, Junming Yue, Zoltán Benyó, Gábor J. Tigyi

“…Because B16-F10 cells express high levels of ATX, we used the CRISPR-Cas9 technology to knock out the ATX gene in B16-F10 cells, eliminating the contribution of tumor-derived ATX in lung metastasis. …”
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Sex-related differences in the response of anti-platelet drug therapies targeting purinergic signaling pathways in sepsis by Emmanuel Boadi Amoafo, Philomena Entsie, Samara Albayati, Glenn P. Dorsam, Satya P. Kunapuli, Laurie E. Kilpatrick, Elisabetta Liverani, Elisabetta Liverani

“…Male and female wild-type (WT), P2Y12 knock-out (KO), and P2Y1 KO mice underwent sham surgery or cecal ligation and puncture (CLP) to induce sepsis. …”
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ICAMs are dispensable for influenza clearance and anti-viral humoral and cellular immunity by Stav Kozlovski, Ofer Regev, Anita Sapoznikov, Marina Kizner, Hagit Achdout, Ekaterina Petrovich-Kopitman, Jacob Elkahal, Yoseph Addadi, Fernanda Vargas E. Silva Castanheira, Sara W. Feigelson, Paul Kubes, Noam Erez, Natalio Garbi, Ronen Alon

“…Surprisingly, double ICAM-1 and ICAM-2 knock out mice (herein ICAM-1/2-/- mice) infected with a lab adapted H1N1 influenza A virus fully recovered from infection, elicited potent humoral immunity, and generated normal long lasting anti-viral CD8+ T cell memory. …”
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Functional Genomic Analyses of the 21q22.3 Locus Identifying Functional Variants and Candidate Gene <i>YBEY</i> for Breast Cancer Risk by Chris Shidal, Xiang Shu, Jie Wu, Jifeng Wang, Shuya Huang, Jirong Long, Joshua A. Bauer, Jie Ping, Xingyi Guo, Wei Zheng, Xiao-Ou Shu, Qiuyin Cai

“…We investigated the biological effects of <i>YBEY</i> on breast cancer cell lines by transient knock-down of <i>YBEY</i> expression in MCF-7, T47D, and MDA-MB-231 cell lines. …”
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Protein Kinase D Plays a Crucial Role in Maintaining Cardiac Homeostasis by Regulating Post-Translational Modifications of Myofilament Proteins by Melissa Herwig, Merima Begovic, Heidi Budde, Simin Delalat, Saltanat Zhazykbayeva, Marcel Sieme, Luca Schneider, Kornelia Jaquet, Andreas Mügge, Ibrahim Akin, Ibrahim El-Battrawy, Jens Fielitz, Nazha Hamdani

“…To do this, we used mice with a cardiomyocyte-specific knock-out of Prkd1, which encodes PKD1 (Prkd1^loxP/loxP; ^αMHC-Cre; PKD1 cKO), as well as wild type littermate controls (Prkd1^loxP/loxP; WT). …”
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Construction of translocase of inner mitochondrial membrane 8A gene knockout mice and study of its inner ear function by HONG Hanxin, WANG Longhao, LIU Huihui, PENG Hu, WU Hao, YANG Tao

“…Transmission electron microscope was used to observe the ultrastructure of mitochondria in inner ear hair cells (HCs), SGN cells and SV cells of the two kinds of mice, and the proportion of abnormal mitochondria was counted.Results·The results of PCR showed that Timm8a gene had been successfully knocked out, and the results of Western blotting showed that there was no TIMM8A proteins in the cochlea, brain, heart and skeletal muscle tissues. …”
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Research on the quality markers of antioxidant activity of Kai-Xin-San based on the spectrum–effect relationship by Xiaoxiao Shan, Xiaoxiao Shan, Xiaoxiao Shan, Xiaoxiao Shan, Xiaoxiao Shan, Xiaoxiao Shan, Xuan Yang, Xuan Yang, Xuan Yang, Xuan Yang, Xuan Yang, Xuan Yang, Dawei Li, Dawei Li, Dawei Li, Dawei Li, Dawei Li, Dawei Li, Lele Zhou, Lele Zhou, Lele Zhou, Lele Zhou, Lele Zhou, Lele Zhou, Shaogang Qin, Shaogang Qin, Junying Li, Junying Li, Junying Li, Junying Li, Junying Li, Junying Li, Wenkang Tao, Wenkang Tao, Wenkang Tao, Wenkang Tao, Wenkang Tao, Wenkang Tao, Can Peng, Can Peng, Can Peng, Can Peng, Can Peng, Can Peng, Jinming Wei, Jinming Wei, Jinming Wei, Jinming Wei, Jinming Wei, Jinming Wei, Xiaoqin Chu, Xiaoqin Chu, Xiaoqin Chu, Xiaoqin Chu, Xiaoqin Chu, Xiaoqin Chu, Haixuan Wang, Haixuan Wang, Caiyun Zhang, Caiyun Zhang, Caiyun Zhang, Caiyun Zhang, Caiyun Zhang, Caiyun Zhang

“…However, the antioxidant activity of KXS was decreased significantly after Q-makers were knocked out.Conclusion: In conclusion, the metabolites in KXS were successfully identified by UPLC-Q-Exactive Orbitrap MS/MS, and the Q-makers of KXS for antioxidant effect was analyzed based on the spectrum-effect relationship. …”
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GRK2–YAP signaling is implicated in pulmonary arterial hypertension development by Peng Ye, Yunfei Deng, Yue Gu, Pengfei Liu, Jie Luo, Jiangqin Pu, Jingyu Chen, Yu Huang, Nanping Wang, Yong Ji, Shaoliang Chen, Rongman Jia, Xiuyuan Hao

“…In vitro study further confirmed that GRK2 knock-down significantly altered hypoxia-induced PASMCs proliferation and migration, whereas this effect was severely intensified by overexpression of GRK2. …”
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Neuropathy-associated Fars2 deficiency affects neuronal development and potentiates neuronal apoptosis by impairing mitochondrial function by Xihui Chen, Fangfang Liu, Bowen Li, Yufeng Wang, Lijuan Yuan, Anan Yin, Qi Chen, Weihong Hu, Yan Yao, Mengjie Zhang, YuanMing Wu, Kun Chen

“…Zebrafish, in which fars2 was knocked down, exhibited aberrant motor neuron function including reduced locomotor capacity which well restored the spastic paraplegia phenotype of FARS2-deficient patients. …”
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Integrated ATAC-Seq and RNA-Seq Data Analysis to Reveal <i>OsbZIP14</i> Function in Rice in Response to Heat Stress by Fuxiang Qiu, Yingjie Zheng, Yao Lin, Samuel Tareke Woldegiorgis, Shichang Xu, Changqing Feng, Guanpeng Huang, Huiling Shen, Yinying Xu, Manegdebwaoga Arthur Fabrice Kabore, Yufang Ai, Wei Liu, Huaqin He

“…Through comprehensive bioinformatics analysis, we demonstrated that <i>OsbZIP14</i>, one of the key heat-responsive TF genes, contained a basic-leucine zipper domain and primarily functioned as a nuclear TF with transcriptional activation capability. By knocking out the <i>OsbZIP14</i> gene in the rice cultivar Zhonghua 11, we observed that the knockout mutant <i>OsbZIP14</i> exhibited dwarfism with reduced tiller during the grain-filling stage. …”
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A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger by Yangyang Li, Cen Li, Yishan Fu, Quan Zhang, Jianing Ma, Jingwen Zhou, Jianghua Li, Guocheng Du, Song Liu

“…In our previous study, a visual multigene editing system (VMS) was designed to knock out five genes, employing a tRNA-sgRNA array that includes the pigment gene albA and the target genes. …”
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GhPYL9-5D and GhPYR1-3 A positively regulate Arabidopsis and cotton responses to ABA, drought, high salinity and osmotic stress by Yibin Wang, Gaofeng Zhang, Huimin Zhou, Shanshan Yin, Yunxiang Li, Caixia Ma, Pengyun Chen, Lirong Sun, Fushun Hao

“…Moreover, the VIGS (Virus-induced gene silencing) cotton plants, in which GhPYL9-5D or GhPYR1-3A were knocked down, showed clearly reduced tolerance to polyethylene glycol 6000 (PEG)-induced drought, salinity and osmotic stresses compared with the controls. …”
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All-trans retinoic acid regulates the expression of MMP-2 and TGF-β2 via RDH5 in retinal pigment epithelium cells by Yu-Mei Mao, Chang-Jun Lan, Qing-Qing Tan, Gui-Mei Zhou, Xiao-Ling Xiang, Jia Lin, Xuan Liao

“…The knockdown efficiency of RDH5 siRNA varies with different targets, among which RDH5 siRNA-435 had the highest knockdown efficiency, i.e., more than 50% lower than that of the negative control group (P=0.02). When RDH5 was knocked down for 48h, the results of qRT-PCR showed that the expressions of MMP-2 and TGF-β2 mRNA were significantly up-regulated (P<0.001). …”
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Pathological α-synuclein recruits LRRK2 expressing pro-inflammatory monocytes to the brain by Enquan Xu, Ravindra Boddu, Hisham A. Abdelmotilib, Arpine Sokratian, Kaela Kelly, Zhiyong Liu, Nicole Bryant, Sidhanth Chandra, Samantha M. Carlisle, Elliot J. Lefkowitz, Ashley S. Harms, Etty N. Benveniste, Talene A. Yacoubian, Laura A. Volpicelli-Daley, David G. Standaert, Andrew B. West

“…LRRK2 expression and LRRK2-dependent Rab10 phosphorylation were measured with monoclonal antibodies, and myeloid cell responses to α-synuclein fibrils in R1441C-Lrrk2 knock-in mice or G2019S-Lrrk2 BAC mice were evaluated by flow cytometry. …”
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A New Crustin Gene Homolog SpCrus8 Identified in Scylla paramamosain Exerting In Vivo Protection Through Opsonization and Immunomodulation by Manyu Jiang, Roushi Chen, Fangyi Chen, Fangyi Chen, Fangyi Chen, Xuewu Zhu, Ke-Jian Wang, Ke-Jian Wang, Ke-Jian Wang

“…When the SpCrus8 gene was knocked down, the expression levels of the key phagocytosis-related genes SpRab5 and SpRab7 were significantly downregulated, as well as the IMD signaling pathway genes SpIKKβ and SpRelish, and another crustin gene SpCrus5. …”
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