The Princeton reader : contemporary essays by writers and journalists at Princeton University / by McPhee, John, 1931-, Rigolot, Carol., Princeton University.

Antagonistic Roles of the Tumor Suppressor miR-210-3p and Oncomucin MUC4 Forming a Negative Feedback Loop in Pancreatic Adenocarcinoma by Nihad Boukrout, Mouloud Souidi, Fatima Lahdaoui, Belinda Duchêne, Bernadette Neve, Lucie Coppin, Emmanuelle Leteurtre, Jérôme Torrisani, Isabelle Van Seuningen, Nicolas Jonckheere

“…These biological effects were validated in cells overexpressing or knocked-down for miR-210-3p. Finally, we showed that miR-210-3p inhibits pancreatic tumor growth and proliferation in vivo. …”
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Sirt1 protects against intervertebral disc degeneration induced by 1,25-dihydroxyvitamin D insufficiency in mice by inhibiting the NF-κB inflammatory pathway by Peng Wang, Cuicui Yang, Jinhong Lu, Yongxin Ren, David Goltzman, Dengshun Miao

“…A vitamin D receptor (VDR)-deficient cellular model was generated by knock-down of endogenous VDR using Ad-siVDR transfection into nucleus pulposus cells; VDR-deficient nucleus pulposus cells were then treated with or without resveratrol. …”
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Editorial: Politics is not a spectator sport: On the role of psycholinguists in a global crisis by Joshua Hartshorne

“…Perhaps, in the face of societal threats, our skills are entirely extraneous, and our time is best spent knocking doors, calling representatives (if we are so fortunate as to have representatives), feeding refugees, comforting the afflicted, trading in our cars for bicycles, or heading to the battlefield. …”
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The genetic and epigenetic regulation of CD55 and its pathway analysis in colon cancer by Jiawei Liu, Jiawei Liu, Ning Fu, Zhenbang Yang, Ang Li, Hongjiao Wu, Ye Jin, Qinqin Song, Shanshan Ji, Hongxue Xu, Zhi Zhang, Xuemei Zhang, Xuemei Zhang

“…Dual-luciferase reporter assays showed that after knocking down TFCP2 or inhibiting NF-κB, the promoter activity of CD55 was decreased by 21% and 70%, respectively; after activating NF-κB, the promoter activity of CD55 increased by 2.3 times. …”
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Interferon-tau protects bovine endometrial epithelial cells against inflammatory injury by regulating the PI3K/AKT/β-catenin/FoxO1 signaling axis by Kangfeng Jiang, Jinyin Cai, Qianming Jiang, Juan J. Loor, Ganzhen Deng, Xiaobing Li, Jing Yang

“…To explore the role of FoxO1 on the inhibition of TLR4 by IFN-τ, we employed LY294002 to inhibit the PI3K/AKT while FoxO1 was knocked down. Results revealed that the knockdown of FoxO1 blocked the upregulation of TLR4 and p-p65 induced by LY294002, and enhanced the inhibition of IFN-τ on TLR4, p-p65, and cell apoptosis. …”
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Small Cajal Body-Specific RNA12 Promotes Carcinogenesis through Modulating Extracellular Matrix Signaling in Bladder Cancer by Qinchen Lu, Jiandong Wang, Yuting Tao, Jialing Zhong, Zhao Zhang, Chao Feng, Xi Wang, Tianyu Li, Rongquan He, Qiuyan Wang, Yuanliang Xie

“…Moreover, SCARNA12 was stably knocked down in T24 and UMUC3 cell lines by lentivirus-mediated CRISPR/Cas9 approach. …”
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Cyclosporine A Modulates LSP1 Protein Levels in Human B Cells to Attenuate B Cell Migration at Low O₂ Levels by Shannon P. Hilchey, Mukta G. Palshikar, Eric S. Mendelson, Shichen Shen, Sailee Rasam, Jason A. Emo, Jun Qu, Juilee Thakar, Martin S. Zand

“…Lastly, we directly link LSP1 levels to chemotactic capacity, as shRNA knock-down of LSP1 results in significantly increased B cell chemotaxis at low O<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msub><mrow></mrow><mn>2</mn></msub></semantics></math></inline-formula> levels. …”
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Metabolic Reprogramming in Response to Alterations of Mitochondrial DNA and Mitochondrial Dysfunction in Gastric Adenocarcinoma by Tzu-Ching Chang, Hui-Ting Lee, Siao-Cian Pan, Shih-Han Cho, Chieh Cheng, Liang-Hung Ou, Chia-I Lin, Chen-Sung Lin, Yau-Huei Wei

“…Parental (PT), null-target (NT), and TFAM-KD-(A/B/C) represented the parental, control, and TFAM knocked-down AGS cells, respectively. These cells were used to compare the parameters reflecting mitochondrial biogenesis, glycolysis, and cell migration activity. …”
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Growth arrest and DNA damage‐inducible alpha regulates muscle repair and fat infiltration through ATP synthase F1 subunit alpha by Wenjing You, Shiqi Liu, Jianfei Ji, Defeng Ling, Yuang Tu, Yanbing Zhou, Wentao Chen, Teresa G. Valencak, Yizhen Wang, Tizhong Shan

“…The adipocyte‐specific GADD45A knock‐in (KI) mice and high IMAT‐infiltrated muscle model by glycerol injection (50 μL of 50% v/v GLY) were generated. …”
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C/EBPα-p30 confers AML cell susceptibility to the terminal unfolded protein response and resistance to Venetoclax by activating DDIT3 transcription by Mengbao Du, Mowang Wang, Meng Liu, Shan Fu, Yu Lin, Yankun Huo, Jian Yu, Xiaohong Yu, Chong Wang, Haowen Xiao, Limengmeng Wang

“…Methods Human AML cell lines and murine myeloid precursor cell line 32Dcl3 cells were infected with recombinant lentiviruses to knock down CEBPA expression or over-express the two isoforms of C/EBPα. …”
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Epidemiological drivers of transmissibility and severity of SARS-CoV-2 in England by Pablo N. Perez-Guzman, Edward Knock, Natsuko Imai, Thomas Rawson, Yasin Elmaci, Joana Alcada, Lilith K. Whittles, Divya Thekke Kanapram, Raphael Sonabend, Katy A. M. Gaythorpe, Wes Hinsley, Richard G. FitzJohn, Erik Volz, Robert Verity, Neil M. Ferguson, Anne Cori, Marc Baguelin

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Genome-Wide CRISPR Screen Identifies Regulators of Mitogen-Activated Protein Kinase as Suppressors of Liver Tumors in Mice by Song, Chun-Qing, Li, Yingxiang, Mou, Haiwei, Moore, Jill, Park, Angela, Pomyen, Yotsawat, Hough, Soren, Kennedy, Zachary, Fischer, Andrew, Yin, Hao, Anderson, Daniel Griffith, Conte, Darryl, Zender, Lars, Wang, Xin Wei, Thorgeirsson, Snorri, Weng, Zhiping, Xue, Wen

“…Genes found to be up-regulated after tumor suppressor loss were examined in liver cancer cell lines; their expression was knocked down using small hairpin RNAs, and tumor growth was examined in nude mice. …”
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Risk-based route planning and decision making for unmanned aircraft system operations in urban environments by Pang, Bizhao

“…Airborne collisions between drones yield air risk, and the falling of collided drones causes knock-on ground risk. These safety risks could result in severe casualties to third-party people and considerable property loss to urban infrastructures, which need immediate research efforts to assess and mitigate the safety risks. …”
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The role of EZH2 in paediatric myelodysplasia by Fordham, N

“…Developing a mouse model to study the fetal origins of JMML: Initial experiments with Vav-iCre induction of mutations demonstrated the proof of principle that Kras^G12D mutant cells from the fetal liver (FL) can cause a myelomonocytic leukaemia. Ezh2 knock-out (KO) in combination with Kras^G12D, however, led to a cellular defect with no engraftment. …”

Cancer‐associated fibroblasts nurture LGR5 marked liver tumor‐initiating cells and promote their tumor formation, growth, and metastasis by Mingna Zhang, Yiqiao Fang, Xia Fu, Jiaye Liu, Yang Liu, Zhounan Zhu, Yinyun Ni, Menglin Yao, Qiuwei Pan, Wanlu Cao, Zhihui Li, Chunyan Dong

“…Materials and Methods First, both of the transgenic LGR5‐diphtheria toxin receptor (DTR)‐GFP knock‐in mice and transgenic Rosa26‐mT mice developed primary liver tumors by diethylnitrosamine (DEN) administration. …”
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CaMKK2 is not involved in contraction-stimulated AMPK activation and glucose uptake in skeletal muscle by Florentina Negoita, Alex B. Addinsall, Kristina Hellberg, Conchita Fraguas Bringas, Paul S. Hafen, Tyler J. Sermersheim, Marianne Agerholm, Christopher T.A. Lewis, Danial Ahwazi, Naomi X.Y. Ling, Jeppe K. Larsen, Atul S. Deshmukh, Mohammad A. Hossain, Jonathan S. Oakhill, Julien Ochala, Jeffrey J. Brault, Uma Sankar, David H. Drewry, John W. Scott, Carol A. Witczak, Kei Sakamoto

“…Methods: A recently developed CaMKK2 inhibitor (SGC-CAMKK2-1) alongside a structurally related but inactive compound (SGC-CAMKK2-1N), as well as CaMKK2 knock-out (KO) mice were used. In vitro kinase inhibition selectivity and efficacy assays, as well as cellular inhibition efficacy analyses of CaMKK inhibitors (STO-609 and SGC-CAMKK2-1) were performed. …”
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Functional characterization of RhuB as a second TonB2-dependent hemin receptor in Riemerella anatipestifer CH-1 by Mengying Wang, Siyi Wang, Mingshu Wang, Dekang Zhu, Renyong Jia, Shun Chen, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Mafeng Liu, Anchun Cheng

“…Herein, we demonstrated that R. anatipestifer hemin uptake receptor B (RhuB) of R. anatipestifer CH-1 (RA CH-1) was negatively regulated by iron and mediated by the Fur protein, and knocking out rhuB damaged the ability of RA CH-1 to utilize iron from duck hemoglobin (Hb) but not that from duck serum. …”
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Discrete and Continuous Operational Calculus in N-Critical Shocks Reliability Systems with Aging under Delayed Information by Jewgeni H. Dshalalow, Hend Aljahani

“…It takes a total of <i>N</i> critical shocks to knock the system down. In addition, the system ages in accordance with a monotone increasing continuous function <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>δ</mi></semantics></math></inline-formula>, so that when <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>δ</mi><mfenced open="(" close=")"><mi>T</mi></mfenced></mrow></semantics></math></inline-formula> crosses some sustainability threshold <i>D</i> at time <i>T</i>, the system becomes essentially inoperational. …”
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O-GlcNAcylation regulates extracellular signal-regulated kinase (ERK) activation in Alzheimer’s disease by Sophiya John Ephrame, Gentry K. Cork, Victoria Marshall, Margaret A. Johnston, Jenna Shawa, Ibtihal Alghusen, Amy Qiang, Aspin R. Denson, Marisa S. Carman, Halyna Fedosyuk, Russell H. Swerdlow, Russell H. Swerdlow, Russell H. Swerdlow, Chad Slawson, Chad Slawson

“…However, O-GlcNAc quickly returns to a baseline level after stimulus removal (called O-GlcNAc homeostasis).MethodsWe did a serum reactivation time-course followed by western blot in SH-SY5Y neuroblastoma cells after long-term O-GlcNAcase (OGA) inhibition by Thiamet-G (TMG) treatment, O-GlcNAc transferase (OGT) knock-down (KD) and OGA KD. Brain tissues of C57BL6/J mice and 5XFAD Alzheimer’s disease mice intra-peritoneally injected with TMG for 1 month and C57BL6/J mice intra-peritoneally injected with TMG for 6 months were also used for western blot.ResultsWe found that ERK1/2 phosphorylation at Thr 202/Tyr204 and Thr183/Tyr185 (p-ERK) are amplified and hence ERK1/2 are activated after long-term OGA inhibition in SH-SY5Y cells. …”
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