Comparison of two methods for dimethylarginines quantification by Vendula Sudová, Pavel Prokop, Ladislav Trefil, Jaroslav Racek, Daniel Rajdl

“…Design and methods: The analysis was performed on a Dionex UltiMate 3000 UHPLC-Standard (Thermo Fisher Scientific, Waltham, Massachusetts, USA) with an amaZon SL ion trap (Bruker, Billerica, Massachusetts, USA). …”
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Роль toll-подобных рецепторов в развитии осложнений после кардиохирургического лечения врожденных пороков сердца у детей... by А. Цепокина, А. Аникеенко, С. Шмулевич, А. Понасенко, А. Шабалдин

“…Генотипирование toll-подобных рецепторов выполняли методом полимеразной цепной реакции в режиме реального времени с использованием зондов TaqMan (Applied Biosystems, Thermo Fisher Scientific, Уолтем, США). Результаты. Оптимальным межгенным взаимодействием является двухлокусная модель TLR1 rs5743551 – TLR2 rs3804099, на долю которой пришлось 4,01 % фенотипической энтропии. …”
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Anion exchange HPLC monitoring of mRNA in vitro transcription reactions to support mRNA manufacturing process development by Emma N. Welbourne, Kate A. Loveday, Adithya Nair, Ehsan Nourafkan, Jixin Qu, Ken Cook, Zoltán Kis, Zoltán Kis, Mark J. Dickman

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Exposición de flúor durante el embarazo y sus posibles efectos a la salud en la vida intrauterina y neonatos de Durango México by Leslie Gamboa Guerrero, Rogelio González González, Oscar Eduardo Almeda Ojeda, Sergio Manuel Salas Pacheco, Nelly Molina Frechero, Omar Alejandro Tremillo Maldonado

“…Se medirá la concentración de fluoruros en tres biomarcadores: orina, plasma sanguíneo y leche materna por medio de potenciometría con electrodo de ion selectivo flúor de Thermo Fisher Scientific siguiendo los protocolos del fabricante. …”
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Derivatives Incorporating Acridine, Pyrrole, and Thiazolidine Rings as Promising Antitumor Agents by Monika Garberová, Ivan Potočňák, Monika Tvrdoňová, Monika Majirská, Martina Bago-Pilátová, Slávka Bekešová, Andrej Kováč, Peter Takáč, Krutika Khiratkar, Zuzana Kudličková, Ján Elečko, Mária Vilková

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Forensic Characterization and Genetic Portrait of the Gannan Tibetan Ethnic Group via 165 AI-SNP Loci by Wei Cui, Man Chen, Hongbing Yao, Qing Yang, Liu Liu, Xiaole Bai, Ling Chen, Bofeng Zhu

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Analysis of long non-coding RNA (lncRNA) expression in hepatitis B patients by Sunde Yılmaz Susluer, Cagla Kayabasi, Besra Ozmen Yelken, Aycan Asik, Didem Celik, Tugce Balci Okcanoglu, Suheyla Serin Senger, Cigir Biray Avci, Sukran Kose, Cumhur Gunduz

“…Chemically synthesized siRNAs were transfected into the cell lines using Lipofectamine 2000 Reagent (Thermo Fisher Scientific). HBV DNA and HBsAg and HBeAg were detected in transfected cultures by real-time PCR and ELISA, respectively, using commercial kits. …”
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3D Cell Culture-Based Global miRNA Expression Analysis Reveals miR-142-5p as a Theranostic Biomarker of Rectal Cancer Following Neoadjuvant Long-Course Treatment by Linas Kunigenas, Vaidotas Stankevicius, Audrius Dulskas, Elzbieta Budginaite, Gediminas Alzbutas, Eugenijus Stratilatovas, Nils Cordes, Kestutis Suziedelis

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Multi-locus evaluation of gastrointestinal bacterial communities from Zalophus californianus pups in the Gulf of California, México by David Ramirez-Delgado, Francesco Cicala, Ricardo A. Gonzalez-Sanchez, Rosalia Avalos-Tellez, Elena Solana-Arellano, Alexei Licea-Navarro

“…To date, the only analytical pipeline available for this kit is the Ion Reporter™ Software of Thermo Fisher Scientific. Therefore, we propose an in-house pipeline to use with open-access tools, such as QIIME2 and PICRUSt 2, in downstream bioinformatic analyses. …”
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Eosinophilic Esophagitis: Cytokines Expression and Fibrotic Markers in Comparison to Celiac Disease by Annamaria Pronio, Francesco Covotta, Lucia Pallotta, Rossella Palma, Danilo Badiali, Maria Carlotta Sacchi, Antonietta Lamazza, Carola Severi

“…Quantitative real-time PCR (qRT-PCR) was performed in triplicate using a StepOne™ Real-Time PCR instrument (Thermo Fisher Scientific, Monza, Italy). mRNA encoding for inflammatory molecules: interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 13 (IL-13), and fibrotic markers: transforming growth factor beta 1 (TGF-β), mitogen-activated protein kinase kinase kinase 7 (MAP3K7), serpin family E member 1 (SERPINE1), were quantified using TaqMan Gene Expression Assays (Applied Biosystems). …”
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Whole genome sequencing of an Enterococcus faecalis isolate from a patient with cholecystitis in a tertiary hospital in Kota Kinabalu, Sabah, Malaysia by Nur Nashyiroh Mastor, Vijay Kumar Subbiah, Wan Nazirah Wan Abu Bakar, Suzanah Silee, Khurshida Begum, M. Jahangir Alam, Mohammad Zahirul Hoque

“…The quantification of DNA was done using Qubit fluorometer (Qubit 3.0, Thermo Fisher Scientific). The sample was then sequenced using the Illumina HiSeq 4000 system. …”
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Three-dimensional printing of ramipril tablets by fused deposition modeling by O. A. Terenteva, K. A. Gusev, V. V. Tikhonova, D. N. Maimistov, G. A. Shandryuk, E. V. Flisyuk

“…Ramipril filaments were prepared by hot melt extrusion on the extruder Haake™ miniCTW (Thermo Fisher Scientific). The tablets were printed on a hand-made 3D printer. …”
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Evidence of HIV-1 Genital Shedding after One Year of Antiretroviral Therapy in Females Recently Diagnosed in Bamako, Mali by Abdelaye Keita, Josselin Rigaill, Sylvie Pillet, Youssouf Sereme, Souleymane Coulibaly, Fodé Diallo, Paul Verhoeven, Bruno Pozzetto, Tenin Aoua Thiero, Thomas Bourlet

“…The vaginal microbiota was analyzed using IonTorrent^TM NGS technology (Thermo Fisher Scientific). Proportions of primary drug resistance mutations in blood and CVF were 13.4% and 25%, respectively. …”
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ГЕН-ГЕННЫЕ ВЗАМОДЕЙСТВИЯ ЦИТОКИНОВ И С-РЕАКТИВНОГО БЕЛКА ПРИ ПСОРИАЗЕ У ЖИТЕЛЕЙ КЕМЕРОВСКОЙ ОБЛАСТИ... by Елизавета Romanova Леонидовна, Andrey Vladimirovich Shabaldin, Anastasia Alexandrovna Yakovleva, Evgeny Georgievic Belov, Larisa Vladimirovna Striga, Elena Aleksandrovna Shevchenko, Elena Viktorovna Shabaldina

“…Genotyping was performed by PCR using TaqMan probes (Thermo Fisher Scientific, USA) on the detection amplifier ViiATM 7 Real-Time PCR System (Life Technologies, USA) of the following polymorphic variants of genes: IL1b (rs16944), IL6 (rs1554606), IL8 (rs2227306), IL10 (rs1800896), TNFα (rs361525), CRP (rs1205). …”
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The Antimicrobial Resistance (AMR) Rates of <i>Enterobacterales</i> in a Rural Hospital from the Eastern Region, Ghana: A Retrospective Study, 2022 by Laura Seijas-Pereda, Carlos Rescalvo-Casas, Marcos Hernando-Gozalo, Vida Angmorkie-Eshun, Eunice Agyei, Vivian Adu-Gyamfi, Isaac Sarsah, Maite Alfonso-Romero, Juan Cuadros-González, Juan Soliveri-de Carranza, Ramón Pérez-Tanoira

“…To this purpose, the samples were cultured on agar plates prepared in the laboratory using purified Oxoid™ Thermo Scientific™ agar (Thermo Fisher Scientific; Waltham, MA, USA). Cystine Lactose Electrolyte-Deficient (CLED) agar was used for urine samples, while blood agar, chocolate agar, and MacConkey agar were used for the rest of the specimens tested (HVS, blood, BFA, sputum). …”
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Role of Genetic Polymorphism Present in Macrophage Activation Syndrome Pathway in Post Mortem Biopsies of Patients with COVID-19 by Aline Cristina Zanchettin, Leonardo Vinicius Barbosa, Anderson Azevedo Dutra, Daniele Margarita Marani Prá, Marcos Roberto Curcio Pereira, Rebecca Benicio Stocco, Ana Paula Camargo Martins, Caroline Busatta Vaz de Paula, Seigo Nagashima, Lucia de Noronha, Cleber Machado-Souza

“…Polymorphisms in MAS cytolytic pathway (<i>PRF1</i>; <i>STX11</i>; <i>STXBP2</i>; <i>UNC13D</i> and <i>GZMB</i>) were selected and genotyping by TaqMan^® assays (Thermo Fisher Scientific, MA, USA) using Real-Time PCR (Applied Biosystems, MA USA). …”
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Development of a method for detection of specific antibodies to E protein of yellow fever virus (Flaviviridae: Flavivirus) by enzyme immunoassay by Ekaterina I. Krivosheina, Mikhail Y. Kartashov, Ekaterina V. Naidenova, Nikita D. Ushkalenko, Stepan A. Pyankov, Vladimir A. Ternovoi, Valery B. Loktev

“…A specific cDNA was synthesized by reverse transcription on an RNA template of YFV isolated on a cell culture of Aedes albopictus clone C6/36, and a fragment of the genome coding the YFV E protein was amplified and subsequently cloned into the plasmid pET160 (Thermo Fisher Scientific, USA). The resulting gene fragment was used as a DNA template to obtain a recombinant analog of the third domain of the YFV E protein in Escherichia coli cells (BL-21(DE3)). …”
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Antimicrobial susceptibility and minimum inhibitory concentration distribution of common clinically relevant non-tuberculous mycobacterial isolates from the respiratory tract by Guiqing He, Lianpeng Wu, Qingyong Zheng, Xiangao Jiang

“…The MICs of 81 isolates were determined using the microbroth dilution method (Thermo Fisher Scientific, USA), as recommended by the Clinical and Laboratory Standards Institute, USA.Results: Included were 43 Mycobacterium avium complex (MAC) strains, 24 M. abscessus complex (MAB) strains, and 14 M. kansasii strains. …”
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High microbiome variability in pediatric tracheostomy cannulas in patients with similar clinical characteristics by Leonardo Palma Kuhl, Paulo José Cauduro Marostica, Alexandre José Macedo, Gabriel Kuhl, Marina Siebert, Denise Manica, Leo Sekine, Cláudia Schweiger

“…DNA extraction was performed using DNeasy PowerBiofilm Kit (QIAGEN® ‒ Cat nº 24000-50) while sequencing was performed with the S5 (Ion S5™ System, Thermo Fisher Scientific), following Brazilian Microbiome Project (BMP) protocol. …”
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Clinical performance evaluation of TAQPATH Enteric Bacterial Select Panel for the detection of common enteric bacterial pathogens in comparison to routine stool culture and other q... by Jasmin Koeffer, Melissa Kolb, Oceane Sorel, Camilla Ulekleiv, Jelena D. M. Feenstra, Ulrich Eigner

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