Persistence of Chronic Lymphocytic Leukemia Stem-like Populations under Simultaneous In Vitro Treatment with Curcumin, Fludarabine, and Ibrutinib: Implications for Therapy Resistan... by Àngel Bistué-Rovira, Laura G. Rico, Jorge Bardina, Jordi Juncà, Isabel Granada, Jolene A. Bradford, Michael D. Ward, Roser Salvia, Francesc Solé, Jordi Petriz

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Dataset from a proteomics analysis of tumor antigens shared between an allogenic tumor cell lysate vaccine and pancreatic tumor tissue. by C. Stingl, S.P. Lau, S.H. van der Burg, J.G. Aerts, C.H.J. van Eijck, T.M. Luider

“…To this objective, cell lysates of the vaccine and of nine tissue samples were enzymatically digested and isotopically labeled with tandem mass tags (TMT) in a so-called six-plex manner (Thermo Fisher Scientific). Three pools were prepared by mixing the samples according to their TMT-labels. …”
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Relationship of the recurrence after pterygium surgery with the presence of HSV, EBV, CMV, HPV, and BRAFV600E mutation by S.O. Rykov, K.O Usenko, S.Iu. Mogilevskyy, S.V. Ziablitsev, L.I. Denisiuk

“…Mutation detection was performed using TaqMan Mutation Detection Assays (Thermo Fisher Scientific) in a Real-Time PCR System 7500 (Applied Biosystems, USA). …”
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Setting Up an Ultra-Fast Next-Generation Sequencing Approach as Reflex Testing at Diagnosis of Non-Squamous Non-Small Cell Lung Cancer; Experience of a Single Center (LPCE, Nice, F... by Marius Ilié, Véronique Hofman, Christophe Bontoux, Simon Heeke, Virginie Lespinet-Fabre, Olivier Bordone, Sandra Lassalle, Salomé Lalvée, Virginie Tanga, Maryline Allegra, Myriam Salah, Doriane Bohly, Jonathan Benzaquen, Charles-Hugo Marquette, Elodie Long-Mira, Paul Hofman

“…We report here our experience concerning the development of an optimal workflow for genomic alteration assessment as reflex testing in routine clinical practice at diagnosis for NS-NSCLC patients by using an ultra-fast-next generation sequencing approach (Ion Torrent Genexus Sequencer, Thermo Fisher Scientific). We show that the molecular targets currently available to personalized medicine in thoracic oncology can be identified using this system in an appropriate TAT, notably when only a small amount of nucleic acids is available. …”
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Altered Extracellular Vesicle miRNA Profile in Prodromal Alzheimer’s Disease by Caterina Visconte, Chiara Fenoglio, Maria Serpente, Paola Muti, Andrea Sacconi, Marta Rigoni, Andrea Arighi, Vittoria Borracci, Marina Arcaro, Beatrice Arosio, Evelyn Ferri, Maria Teresa Golia, Elio Scarpini, Daniela Galimberti

“…A panel of 754 miRNAs was determined with RT-qPCR using TaqMan OpenArray technology in a QuantStudio 12K System (Thermo Fisher Scientific). The results demonstrated that plasma EVs showed widespread deregulation of specific miRNAs (miR-106a-5p, miR-16-5p, miR-17-5p, miR-195-5p, miR-19b-3p, miR-20a-5p, miR-223-3p, miR-25-3p, miR-296-5p, miR-30b-5p, miR-532-3p, miR-92a-3p, and miR-451a), some of which were already known to be associated with neurological pathologies. …”
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A comprehensive dataset for assessing the impact of ammonium salts and zeolite on anaerobic digestion performance, microbial dynamics, and metabolomic profiles by Stephany Damaris Campuzano Zagal, Xiaoqing Wang, Nadine Derlet, Angéline Guenne, Chrystelle Bureau, Sophie Thibault, Olivier Chapleur

“…The metabolomic data were generated using an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, MA, US). The metabolomic data have been deposited to the EMBL-EBI MetaboLights database with the identifier MTBLS7859 (https://www.ebi.ac.uk/metabolights/MTBLS7859). …”
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Detection of <i>EGFR</i> Mutations in Plasma Cell-Free Tumor DNA of TKI-Treated Advanced-NSCLC Patients by Three Methodologies: Scorpion-ARMS, PNAClamp, and Digital PCR by Annamaria Siggillino, Paola Ulivi, Luigi Pasini, Maria Sole Reda, Elisa Chiadini, Francesca Romana Tofanetti, Sara Baglivo, Giulio Metro, Lucio Crinó, Angelo Delmonte, Vincenzo Minotti, Fausto Roila, Vienna Ludovini

“…We tested 31 NSCLC patients treated with anti-EGFR tyrosine kinase inhibitors (TKIs), at baseline and serially during therapy, by comparing three methodologies in detecting <i>EGFR</i> mutations (L858R, exon 19 deletion, and T790M) from plasma: scorpions-amplification refractory mutation system (ARMS) methodology by using <i>EGFR</i> Plasma RGQ PCR Kit-QIAGEN, peptide nucleic acid (PNA) clamp and PANA RealTyper integration by using PNAClamp <i>EGFR</i>-PANAGENE, and digital real time PCR by using QuantStudio 3D Digital PCR System-Thermo Fisher Scientific. Specificity was 100% for all three mutations, independently from the platform used. …”
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Development of Orodispersible Ibuprofen Tablets Based on a Polymer-drug Complex by V. R. Timergalieva, R. R. Khusnutdinov, R. R. Musina, E. S. Elizarova, R. R. Alsynbaev, Sh. F. Nasibullin, R. I. Moustafine

“…IR spectra were recorded on a Nicolet iS5 FT-IR spectrometer (Thermo Fisher Scientific, U.S.A.) with an ATR nozzle, in the range from 500 to 4000 cm-1. …”
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ANALYSIS OF DIFFERENTIAL ExPRESSION OF MATRIx METALLOPROTEASES IN STABLE AND UNSTABLE ATHEROSCLEROTIC LESIONS BY A METHOD OF FULL GENOME SEQUENCING OF RNA: PILOT STUDY by D. Е. Ivanoschuk, Yu. I. Ragino, E. V. Shakhtshneider, S. V. Mikhailova, V. S. Fishman, Ya. V. Polonskaya, E. V. Kashtanova, A. M. Chernyavsky, I. S. Murashov, М. I. Voevoda

“…In intima/media homogenates by IEA method, with BCM Diagnostics assays the levels of destructive markers were measured: MMP-1, MMP3, MMP-7, MMP-9, TIMP-1 on the Multiscan EX (Thermo Fisher Scientific, USA). Libraries preparation for full genomic sequencing of RNA was done with Illumina’s TruSeq RNA Sample Preparation Kit (Illumina, USA). …”
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Efficient SARS-CoV-2 Surveillance during the Pandemic-Endemic Transition Using PCR-Based Genotyping Assays by Lianne Koets, Karin van Leeuwen, Maaike Derlagen, Jalenka van Wijk, Nadia Keijzer, Jelena D. M. Feenstra, Manoj Gandhi, Oceane Sorel, Thijs J. W. van de Laar, Marco H. G. M. Koppelman

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Comparative analysis of contemporary anti-double stranded DNA antibody assays for systemic lupus erythematosus by Claus-Juergen Bauer, Pantelis Karakostas, Nadine Weber, Charlotte Behning, Birgit Stoffel-Wagner, Peter Brossart, Ramona Dolscheid-Pommerich, Valentin Sebastian Schäfer

“…For all study participants’ serum samples both anti-dsDNA-NcX enzyme-linked immunoassay testing EUROIMMUN, Luebeck, Germany) and the fluorescence immunoassay ELiA dsDNA (Thermo Fisher Scientific, Waltham, USA) were performed. In addition, demographic data, further laboratory values and disease activity parameters were recorded. …”
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Pesticides and pharmaceuticals data collected during two consecutive years in a Mediterranean micro-estuary by Tom Topaz, Julius Ben-Ari, Evgenia Kertsnus Banchik, Or Bassa, Roey Egozi, Yair Suari, Tal Sade, Hadar Zedaka, Merav Gilboa, Gitai Yahel, Benny Chefetz

“…Chemical analysis was performed using liquid chromatography with high-resolution mass spectrometry (LC–HRMS) analysis using a QExactive Plus hybrid FT mass spectrometer coupled with a Dionex Ultimate 3000 RS UPLC (Thermo Fisher Scientific, Waltham, MA, USA). The targeted analysis, which included 15 fungicides, 25 herbicides, 18 Insecticides, and 19 pharmaceuticals, concluded with a total of 21,142 entries. …”
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Interpolymer Complexes Based on Eudragit^® EPO/Eudragit^® S100 as Carriers for Oral Controlled Drug Delivery Systems by A. V. Bukhovets, A. Y. Sitenkov, R. I. Moustafine

“…The composition was determined using the method of elemental analysis on a Thermo Flash 2000 CHNS/O device (Thermo Fisher Scientific, UK). The swelling capacity of the obtained IPCs was evaluated in buffer media with pH values that mimic the pH of different parts of the gastrointestinal tract (GIT): 1.2; 5.8; 6.8 and 7.4. …”
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Anti-Citrullinated Protein Antibody Reactivity towards Neutrophil-Derived Antigens: Clonal Diversity and Inter-Individual Variation by Alexandra Cîrciumaru, Marcelo Gomes Afonso, Heidi Wähämaa, Akilan Krishnamurthy, Monika Hansson, Linda Mathsson-Alm, Márton Keszei, Ragnhild Stålesen, Lars Ottosson, Charlotte de Vries, Miriam A. Shelef, Vivianne Malmström, Lars Klareskog, Anca I. Catrina, Caroline Grönwall, Aase Hensvold, Bence Réthi

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Blood selectin levels as a predictive factor for diabetic retinopathy and diabetic macular edema in type 2 diabetes by S.O. Rykov, D.I. Chugaiev, S.V. Ziablintsev

“…Enzyme-linked immunosorbent assay (ELISA) kits from Invitrogen Thermo Fisher Scientific (USA) were used to determine selectin levels in blood. …”
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Non-Invasive Profiling of Advanced Prostate Cancer via Multi-Parametric Liquid Biopsy and Radiomic Analysis by Gareth Morrison, Jonathan Buckley, Dejerianne Ostrow, Bino Varghese, Steven Y. Cen, Jeffrey Werbin, Nolan Ericson, Alexander Cunha, Yi-Tsung Lu, Thaddeus George, Jeffrey Smith, David Quinn, Vinay Duddalwar, Timothy Triche, Amir Goldkorn

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Impaired Antibody Response Is Associated with Histone-Release, Organ Dysfunction and Mortality in Critically Ill COVID-19 Patients by Rickard Lagedal, Oskar Eriksson, Anna Sörman, Joram B. Huckriede, Bjarne Kristensen, Stephanie Franzén, Anders Larsson, Anders Bergqvist, Kjell Alving, Anders Forslund, Barbro Persson, Kristina N. Ekdahl, Pablo Garcia de Frutos, Bo Nilsson, Gerry A. F. Nicolaes, Miklos Lipcsey, Michael Hultström, Robert Frithiof

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Association between primary or booster COVID-19 mRNA vaccination and Omicron lineage BA.1 SARS-CoV-2 infection in people with a prior SARS-CoV-2 infection: A test-negative case-con... by Margaret L Lind, Alexander J Robertson, Julio Silva, Frederick Warner, Andreas C Coppi, Nathan Price, Chelsea Duckwall, Peri Sosensky, Erendira C Di Giuseppe, Ryan Borg, Mariam O Fofana, Otavio T Ranzani, Natalie E Dean, Jason R Andrews, Julio Croda, Akiko Iwasaki, Derek A T Cummings, Albert I Ko, Matt D T Hitchings, Wade L Schulz

“…<h4>Methods and findings</h4>We conducted a test-negative case-control study of reverse transcription PCRs (RT-PCRs) analyzed with the TaqPath (Thermo Fisher Scientific) assay and recorded in the Yale New Haven Health system from November 1, 2021, to April 30, 2022. …”
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Evaluation of Three Automated Extraction Systems for the Detection of SARS-CoV-2 from Clinical Respiratory Specimens by Ho-Jae Lim, Hye-Soo Jung, Min-Young Park, Young-Hyun Baek, Balaji Kannappan, Jin-Young Park, Jae-Hyun Yang, Ja-Hwan Seol, Min-Woo Lee, Sun-Kyung Jung, Sun-Hwa Lee, Jung-Eun Park, Yong-Jin Yang

“…Given that efficient nucleic acid extraction greatly affects reliable and accurate testing results, we compared three extraction platforms: MagNA Pure 96 DNA and Viral NA Small Volume kit on MagNA Pure 96 (Roche, Basel, Switzerland), careGENE^TM Viral/Pathogen HiFi Nucleic Acid Isolation kit (WELLS BIO Inc., Seoul, Korea) on KingFisher Flex (Thermo Fisher Scientific, Rocklin, CA, USA), and SGRespi^TM Pure kit (Seegene Inc., Seoul, Korea) on Maelstrom 9600 (Taiwan Advanced Nanotech Inc., Taoyuan, Taiwan). …”
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Fourier Transform Infrared Spectroscopy to Assess the Degree of Alteration of Artificially Aged and Environmentally Weathered Microplastics by Claudia Campanale, Ilaria Savino, Carmine Massarelli, Vito Felice Uricchio

“…FTIR spectra were acquired using a Nicolet Summit FTIR (ThermoFisher Scientific) equipped with an Everest ATR with a diamond Crystal plate and a DTGS KBr detector (wavenumber range 4000–500 cm⁻¹, 32 scans per spectrum, spectral resolution of 4 cm⁻¹). …”
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