Optimizing Transformation Frequency of <i>Cryptococcus neoformans</i> and <i>Cryptococcus gattii</i> Using <i>Agrobacterium tumefaciens</i> by Jianmin Fu, Nohelli E. Brockman, Brian L. Wickes

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Development of a miniaturized Ti-plasmid and helper plasmid system for Agrobacterium-mediated plant transformation by Yuh, Leng Teo, Shu, Ting Chang, Wai, Keat Toh, Xin, Yen Tor, Chai, Ling Ho, Pek, Chin Loh, Hann, Ling Wong

“…Tumor-inducing (Ti) plasmid is the requisite for Agrobacterium-mediated plant transformation. …”

Issue when expressing a recombinant protein under the control of p35S in Nicotiana tabacum BY-2 cells by Catherine Navarre, Rik Orval, Marie Peeters, Nicolas Bailly, François Chaumont

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Infiltration with Agrobacterium tumefaciens Induces Host Defense and Development-Dependent Responses in the Infiltrated Zone by Gail J. Pruss, Eugene W. Nester, Vicki Vance

“…A. tumefaciens cured of the tumor-inducing (Ti) plasmid is slightly defective for induction of the three responses, showing that Ti plasmid-encoded factors produced by the disarmed strains contribute only slightly. …”
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A T-DNA from the Agrobacterium tumefaciens Limited-Host-Range Strain AB2/73 Contains a Single Oncogene by Léon Otten, Julien Schmidt

“…Its tumor-inducing (Ti) plasmid has been found to lack DNA homology to known T-DNAs (L. …”
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Agrobacterium: Nature’s Genetic Engineer by Eugene William Nester

“…The genes associated with T-strand formation and transfer (vir) map to the Ti plasmid and are only expressed when the bacteria are at a plant’s wound site. …”
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Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia by Kuzmanović N., Gašić Katarina, Ivanović M., Prokić Anđelka, Obradović A.

“…Bacteria were isolated from the young tumor tissue on nonselective YMA medium and five representative strains were selected for further identification. Tumorigenic (Ti) plasmid was detected in all strains by PCR using primers designed to amplify the virC pathogenicity gene, producing a 414-bp PCR product. …”
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Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia by Janja LAMOVŠEK, Igor ZIDARIČ, Irena MAVRIČ PLEŠKO, Gregor UREK, Stanislav TRDAN

“…Tumor-inducing capacity of these strains was assessed on test plants and with PCR methods for detection of the Ti plasmid responsible for tumor induction. With VCF3/VCR3 primer pair six false negatives and no false positives were detected. …”
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Sugar Beet Improvement using Agrobacterium-mediated Transformation technology by Abo-Bakr A. Youssef, Wessam M. Rslan

“…To achieve this, the tumor-inducing (Ti) plasmid was changed to extract phytohormone and opine biosynthetic proteins (cytokinin and auxin) so as not to interfere with ordinary morphological growth. …”
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A simple and stable method of tagging <em>Agrobacterium fabrum</em> C58 for environmental monitoring by Meriam BOURI, Haifa Ben GHARSA, Ludovic VIAL, Céline LAVIRE, Bernard R. GLICK, Ali RHOUMA

“…Agrobacterium fabrum is one of the eleven Agrobacterium spp. complex species that has been observed to carry a Ti plasmid and induce crown gall, a disease causing significant damage to economically important perennial agricultural crops. …”
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Application of CRISPR/Cas9 genome editing system for molecular breeding of orchids by Endang Semiarti, Sri Nopitasari, Yuli Setiawati, Muhammad Dylan Lawrie, Aziz Purwantoro, Jaka Widada, Yasushi Yoshioka, Shogo Matsumoto, Kana Ninomiya, Yuuki Asano

“…Protocorm were submerged in the culture of Agrobacterium tumefaciens that Ti‐plasmid had been filled with a T‐DNA construct of a pRGEB32 vector harboring sgRNA with PDS3 sequence. …”
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Transition to quorum sensing in an Agrobacterium population: A stochastic model. by Andrew B Goryachev, Da-Jun Toh, Keng Boon Wee, Travis Lee, Hai-Bao Zhang, Lian-Hui Zhang

“…We construct a model describing the Ti plasmid quorum-sensing gene network and demonstrate that it behaves as an "on-off" gene expression switch that is robust to molecular noise and that activates the plasmid conjugation program in response to the increase in autoinducer concentration. …”
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Standardizing a protocol for GMO detection: evaluation of the presence of GMOs in soybeans collected in different collection centers in Ecuador by María de Lourdes Torres P., Lorena Mejía, Venancio S. Arahana B.

“…Primers used for the amplification corresponded to specific sequences of the two most representative recombinant elements in GM (genetically modified) crops, the 35S promoter from the Cauliflower Mosaic Virus (P35S) and NOS terminator from Ti plasmid in <em>Agrobacterium tumefaciens</em> (TNOS). …”
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Crown Gall Disease in Moroccan Almond Trees: Tumorigenic Bacteria and Sustainable Management through Biological Control by Khaoula Habbadi, Hiba Yahyaoui, Salma Benchlih, Abdellatif Benbouazza, Salma El Iraqui EL Houssaini

“…Molecular identification was done using a duplex PCR (A/C9 and A/E9) targeting virD2gene located in the pTi plasmid, which is the oncogenic element of the bacterium. …”
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Small RNA Deep-Sequencing Analyses Reveal a New Regulator of Virulence in Agrobacterium fabrum C58 by M. Dequivre, B. Diel, C. Villard, O. Sismeiro, M. Durot, J. Y. Coppée, X. Nesme, L. Vial, F. Hommais

“…Novel ways of regulating Ti plasmid functions were investigated by studying small RNAs (sRNAs) that are known to act as posttranscriptional regulators in plant pathogenic bacteria. sRNA-seq analyses of Agrobacterium fabrum C58 allowed us to identify 1,108 small transcripts expressed in several growth conditions that could be sRNAs. …”
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Production of Acyl-Homoserine Lactone Quorum-Sensing Signals by Gram-Negative Plant-Associated Bacteria by Chung Cha, Ping Gao, Yu-Ching Chen, Paul D. Shaw, Stephen K. Farrand

“…The traG::lacZ fusion reporter from the A. tumefaciens Ti plasmid was the single most sensitive and versatile detector of the four. …”
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Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants by Xiao Ding, Lu Yu, Luo Chen, Yujie Li, Jinlun Zhang, Hanyan Sheng, Zhengwei Ren, Yunlong Li, Xiaohan Yu, Shuangxia Jin, Jinglin Cao

“…One of the main limitations of the overexpression is the vector capacity constraint that makes it difficult to express multiple genes using the typical Ti plasmid vectors from Agrobacterium. The CRISPRa system can overcome these limitations of the traditional gene overexpression method and achieve multiple gene activation by simply designating several guide RNAs in one vector. …”
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