Bcl-xL is required to protect endothelial cells latently infected with KSHV from virus induced intrinsic apoptosis. by Lyndsey N Moore, Daniel L Holmes, Anjali Sharma, Joselyn Landazuri Vinueza, Michael Lagunoff

“…In this study we rescreened top hits and found that the highest scoring gene necessary for infected cell survival is the anti-apoptotic Bcl-2 family member Bcl-xL. …”
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Identification of BACE-1 Inhibitors against Alzheimer’s Disease through E-Pharmacophore-Based Virtual Screening and Molecular Dynamics Simulation Studies: An Insilco Approach by Kumarappan Chidambaram

“…The identified screened hits were filtered for further analyses using structure-based virtual screening and MM/GBSA. From the analyses, top hits such as ZINC39592220 and en1003sfl.46293 are selected based on their top docking scores (−8.182 and −7.184 Kcal/mol, respectively) and binding free energy (−58.803 and −56.951 Kcal/mol, respectively). …”
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Nutritional compensation of the circadian clock is a conserved process influenced by gene expression regulation and mRNA stability. by Christina M Kelliher, Elizabeth-Lauren Stevenson, Jennifer J Loros, Jay C Dunlap

“…To test for conservation in cultured human cells, we selected top hits from our fungal genetic screen, performed siRNA knockdown experiments of the mammalian orthologs, and characterized the cell lines with respect to compensation. …”
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A Bioluminescent 3CL^Pro Activity Assay to Monitor SARS-CoV-2 Replication and Identify Inhibitors by Cyrille Mathieu, Franck Touret, Clémence Jacquemin, Yves L. Janin, Antoine Nougairède, Manon Brailly, Magalie Mazelier, Didier Décimo, Virginie Vasseur, Aymeric Hans, José-Carlos Valle-Casuso, Xavier de Lamballerie, Branka Horvat, Patrice André, Mustapha Si-Tahar, Vincent Lotteau, Pierre-Olivier Vidalain

“…In agreement with recent reports, inhibitors of pyrimidine biosynthesis were found to prevent SARS-CoV-2 replication. Among the top hits, we also identified the NADPH oxidase (NOX) inhibitor Setanaxib. …”
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Discovery of Novel Inhibitor for WNT/β-Catenin Pathway by Tankyrase 1/2 Structure-Based Virtual Screening by Bo Li, Jinxia Liang, Feng Lu, Guandi Zeng, Jindao Zhang, Yinxing Ma, Peng Liu, Qin Wang, Qian Zhou, Liang Chen

“…After screening more than 13.4 million compounds through molecular docking, we experimentally verified one compound, LZZ-02, as the most potent inhibitor out of 11 structurally representative top hits. LiCl-induced HEK293 cells containing TOPFlash reporter showed that LZZ-02 inhibited the transcriptional activity of β-catenin with an IC₅₀ of 10 ± 1.2 μM. …”
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Discovery of a Potential HER2 Inhibitor from Natural Products for the Treatment of HER2-Positive Breast Cancer by Jianzong Li, Haiyang Wang, Junjie Li, Jinku Bao, Chuanfang Wu

“…In total, 11,247 natural compounds were screened. The top hits were evaluated by an in vitro HER2 kinase inhibition assay. …”
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Drug sensitivity and resistance testing identifies PLK1 inhibitors and gemcitabine as potent drugs for malignant peripheral nerve sheath tumors by Matthias Kolberg, Jarle Bruun, Astrid Murumägi, John P. Mpindi, Christian H. Bergsland, Maren Høland, Ina A. Eilertsen, Stine A. Danielsen, Olli Kallioniemi, Ragnhild A. Lothe

“…We evaluated the effect of 299 clinical and investigational compounds on seven MPNST cell lines, two primary cultures of human Schwann cells, and five normal bone marrow aspirates, to identify potent drugs for MPNST treatment with few side effects. Top hits included Polo‐like kinase 1 (PLK1) inhibitors (volasertib and BI2536) and the fluoronucleoside gemcitabine, which were validated in orthogonal assays measuring viability, cytotoxicity, and apoptosis. …”
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Identifying HSV-1 Inhibitors from Natural Compounds via Virtual Screening Targeting Surface Glycoprotein D by Jiadai Wu, Helen Power, Monica Miranda-Saksena, Peter Valtchev, Aaron Schindeler, Anthony L. Cunningham, Fariba Dehghani

“…More than 527,000 natural compounds were virtually screened using Autodock Vina and then filtered for favorable ADMET profiles. Eight top hits were evaluated experimentally in African green monkey kidney cell line (VERO) cells, which yielded two compounds with potential antiherpetic activity. …”
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Genome-Wide CRISPR Screening Identifies DCK and CCNL1 as Genes That Contribute to Gemcitabine Resistance in Pancreatic Cancer by Hai Yang, Bin Liu, Dongxue Liu, Zhirong Yang, Shuman Zhang, Pengyan Xu, Yuming Xing, Isabella Kutschick, Susanne Pfeffer, Nathalie Britzen-Laurent, Robert Grützmann, Christian Pilarsky

“…We performed genome-wide CRISPR/Cas9 knockout screening in the mouse pancreatic cancer cell line TB32047 with gemcitabine treatment and identified deoxycytidine kinase (DCK) and cyclin L1 (CCNL1) as the top hits. We knocked out DCK and CCNL1 in the TB32047 and PANC1 cell lines and confirmed that the loss of DCK or CCNL1 enhanced gemcitabine resistance in pancreatic cells. …”
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Fragment-Based Approaches Identified Tecovirimat-Competitive Novel Drug Candidate for Targeting the F13 Protein of the Monkeypox Virus by Yasir Ali, Hina Imtiaz, Muhammad Mutaal Tahir, Fouzia Gul, Umair Ali Khan Saddozai, Ashfaq ur Rehman, Zhi-Guang Ren, Saadullah Khattak, Xin-Ying Ji

“…These compounds were evaluated to identify the most promising fragments based on binding affinity and pharmacological characteristics. The top hits from the chemical screening were docked into the active site of the F13 protein. …”
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The clusterin connectome: Emerging players in chondrocyte biology and putative exploratory biomarkers of osteoarthritis by Patrik Kovács, Peter Natesan Pushparaj, Peter Natesan Pushparaj, Roland Takács, Ali Mobasheri, Ali Mobasheri, Ali Mobasheri, Ali Mobasheri, Csaba Matta

“…We also analyzed transcription factors known to modulate clusterin expression, which may be altered in OA.ResultsThe top hits in the clusterin network were intracellular chaperones, aggregate-forming proteins, apoptosis regulators and complement proteins. …”
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Identification of 1H-purine-2,6-dione derivative as a potential SARS-CoV-2 main protease inhibitor: molecular docking, dynamic simulations, and energy calculations by Hossam Nada, Ahmed Elkamhawy, Kyeong Lee

“…The generated hits were ranked using a structure based virtual screening technique (SBVS), and the top hits were subjected to in-depth molecular docking studies and MM-GBSA calculations over SARS-COV-2 Mpro. …”
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An Introduction to Indonesian Wild Shiitake by Ivan Permana Putra, Mada Triandala Sibero, Saipul Sihotang, Lilis Supratman, Rudy Hermawan, Oktan Dwi Nurhayat

“…The BLAST result revealed that our specimen has high similarity (99-100%) with L. lateritia and L. edodes as the top hits. The phylogenetic tree (RAxML) nested our specimens in the L. lateria clade and is closely related to one specimen from Papua New Guenia (PNG) (BS 98%). …”
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Candidate gene sequencing of SLC11A2 and TMPRSS6 in a family with severe anaemia: common SNPs, rare haplotypes, no causative mutation. by Anita Kloss-Brandstätter, Gertraud Erhart, Claudia Lamina, Bernhard Meister, Margot Haun, Stefan Coassin, Markus Seifert, Andreas Klein-Franke, Bernhard Paulweber, Lyudmyla Kedenko, Barbara Kollerits, Dorine W Swinkels, Sita H Vermeulen, Tessel E Galesloot, Florian Kronenberg, Günter Weiss

“…Only two SNPs, which were top-hits from recent GWAS on iron and ferritin, exhibited an effect on iron and ferritin levels in SAPHIR. …”
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Hordatines as a Potential Inhibitor of COVID-19 Main Protease and RNA Polymerase: An In-Silico Approach by Mohammed A. Dahab, Mostafa M. Hegazy, Hatem S. Abbass

“…According to our results, all these top hits was found to have a better binding scores than remdesivir, the native ligand in RNA polymerase target (PDB ID: 7bV2). …”
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High-throughput sequence analysis of turbot (Scophthalmus maximus) transcriptome using 454-pyrosequencing for the discovery of antiviral immune genes. by Patricia Pereiro, Pablo Balseiro, Alejandro Romero, Sonia Dios, Gabriel Forn-Cuni, Berta Fuste, Josep V Planas, Sergi Beltran, Beatriz Novoa, Antonio Figueras

“…Intriguingly, 55.16% of the deduced protein was not significantly similar to any sequences in the databases used for the annotation and only 0.85% of the BLASTx top-hits matched S. maximus protein sequences. This relatively low level of annotation is possibly due to the limited information for this specie and other flatfish in the database. …”
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First Report on Wild Occurrences of Phoenix Mushroom (<i>Pleurotus pulmonarius</i> Fr. Quél.) in Indonesia by Ivan Permana Putra, Oktan Dwi Nurhayat, Mada Triandala Sibero, Rudy Hermawan

“…The BLAST result revealed that our specimens posed a high similarity to P. pulnonarius from several countries as the top hits. The ITS phylogenetic tree placed Pleurotus FIPIA-DEP51 in the same clade of P. pulnonarius with 100% BS value. …”
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Prevalence of IgE‐mediated sensitization in patients with suspected food allergic reactions in Jordan by Issa Abu‐Dayyeh, Jumana Abu‐Kwaik, Alf Weimann, Amid Abdelnour

“…Interestingly, the s‐IgE class distribution profile of pistachio differed from the rest of the top hits being skewed away from the weak class 1 leaning more towards higher IgE classes. …”
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In silico repurposing of antipsychotic drugs for Alzheimer’s disease by Shivani Kumar, Suman Chowdhury, Suresh Kumar

“…In this study, for each protein target, the best drug was identified on the basis of dock score and glide energy. The top hits were then compared with the already known inhibitor of the respective proteins. …”
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Educational attainment: a genome wide association study in 9538 Australians. by Nicolas W Martin, Sarah E Medland, Karin J H Verweij, S Hong Lee, Dale R Nyholt, Pamela A Madden, Andrew C Heath, Grant W Montgomery, Margaret J Wright, Nicholas G Martin

“…This makes EA an attractive alternative phenotype for studies wishing to map genes affecting cognition due to the ease of collecting EA data compared to other cognitive phenotypes such as IQ.In an Australian family sample of 9538 individuals we performed a genome-wide association scan (GWAS) using the imputed genotypes of ∼2.4 million single nucleotide polymorphisms (SNP) for a 6-point scale measure of EA. Top hits were checked for replication in an independent sample of 968 individuals. …”
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