From Compact Discs to Streaming: A Comparison of Eras within the Brazilian Market by Danilo B. Seufitelli, Gabriel P. Oliveira, Mariana O. Silva, Gabriel R. G. Barbosa, Bruna C. Melo, Juliana E. Botelho, Luiza de Melo-Gomes, Mirella M. Moro

“…We find the same clusters for both Physical and Digital eras: Spike Hit Artists, Big Hit Artists, and Top Hit Artists. Our results reveal significant changes in the music industry dynamics over the years by identifying the core of each era.…”
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An in silico analysis identifies drugs potentially modulating the cytokine storm triggered by SARS-CoV-2 infection by Laura Sanchez-Burgos, Gonzalo Gómez-López, Fátima Al-Shahrour, Oscar Fernandez-Capetillo

“…Regarding drugs potentially counteracting the CS, we identified glucocorticoids as a top hit, which validates our approach as this is the primary treatment for this pathology. …”
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Genome-wide CRISPR knockout screen identifies ZNF304 as a silencer of HIV transcription that promotes viral latency. by Simona Krasnopolsky, Alona Kuzmina, Ran Taube

“…Of several top candidates, the KRAB-containing zinc finger protein, ZNF304, was identified as the top hit. ZNF304 silences HIV gene transcription through associating with TRIM28 and recruiting to the viral promoter heterochromatin-inducing methyltransferases, including the polycomb repression complex (PRC) and SETB1. …”
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Chlorambucil targets BRCA1/2‐deficient tumours and counteracts PARP inhibitor resistance by Tacconi, E, Badie, S, De Gregoriis, G, Reisländer, T, Lai, X, Porru, M, Folio, C, Moore, J, Kopp, A, Baguña Torres, J, Sneddon, D, Green, M, Dedic, S, Lee, J, Batra, A, Rueda, O, Bruna, A, Leonetti, C, Caldas, C, Cornelissen, B, Brino, L, Ryan, A, Biroccio, A, Tarsounas, M

“…To identify drugs that target specifically BRCA2-deficient cells, we screened a chemical library containing compounds in clinical use. The top hit was chlorambucil, a bifunctional alkylating agent used for the treatment of chronic lymphocytic leukaemia (CLL). …”

A genome-wide Drosophila epithelial tumorigenesis screen identifies Tetraspanin 29Fb as an evolutionarily conserved suppressor of Ras-driven cancer. by Tamara Zoranovic, Jan Manent, Lee Willoughby, Ricardo Matos de Simoes, John E La Marca, Sofya Golenkina, Xia Cuiping, Susanne Gruber, Belinda Angjeli, Elisabeth Eva Kanitz, Shane J F Cronin, G Gregory Neely, G Gregory Neely, Andreas Wernitznig, Patrick O Humbert, Kaylene J Simpson, Constantine S Mitsiades, Helena E Richardson, Josef M Penninger

“…Among the novel top 80 candidate cancer genes, we mechanistically characterized the function of the top hit, the Tetraspanin family member Tsp29Fb, revealing that Tsp29Fb regulates EGFR signaling, epithelial architecture and restrains tumor growth and invasion. …”
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Pdgfrb is a direct regulatory target of TGFβ signaling in atrioventricular cushion mesenchymal cells. by Yin Peng, Shun Yan, Dongquan Chen, Xiangqin Cui, Kai Jiao

“…Subsequent bioinformatic analysis of TGFβ activated genes revealed that PDGF-BB signaling is the top hit as the potential upstream regulator. Among the 37 target molecules, 10 genes known to be involved in valve development and hemostasis were selected for quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. …”
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MicroRNA Regulation of Human Genes Essential for Influenza A (H7N9) Replication. by Stefan Wolf, Weilin Wu, Cheryl Jones, Olivia Perwitasari, Suresh Mahalingam, Ralph A Tripp

“…Validation studies lead to a top hit, hsa-miR-664a-3p, that had potent antiviral effects in reducing H7N9 replication (TCID50 titers) by two logs. …”
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Imaging-based screen identifies laminin 411 as a physiologically relevant niche factor with importance for i-Hep applications by Ong, J, Serra, M, Segal, J, Cujba, A, Ng, S, Butler, R, Millar, V, Hatch, S, Zimri, S, Koike, H, Chan, K, Bonham, A, Walk, M, Voss, T, Heaton, N, Mitry, R, Dhawan, A, Ebner, D, Danovi, D, Nakauchi, H, Rashid, S

“…The HLI was then applied in a targeted screen of extracellular niche factors to identify substrates driving i-Heps closer to the standard. Laminin 411, the top hit, was validated in two additional induced pluripotent stem cell (iPSC) lines, primary tissue, and an in vitro model of α1-antitrypsin deficiency. …”

Identification of a Pentasaccharide Lead Compound with High Affinity to the SARS-CoV-2 Spike Protein via In Silico Screening by Binjie Li, Tianji Zhang, Hui Cao, Vito Ferro, Jinping Li, Mingjia Yu

“…Molecular modeling was carried out to evaluate the potential antiviral properties of the top-hit pentasaccharide focusing on the interactive regions around the interface of RBD-HS-ACE2. …”
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A CRISPR Screen of HIV Dependency Factors Reveals That <i>CCNT1</i> Is Non-Essential in T Cells but Required for HIV-1 Reactivation from Latency by Terry L. Hafer, Abby Felton, Yennifer Delgado, Harini Srinivasan, Michael Emerman

“…The knockout of Cyclin T1 (<i>CCNT1</i>), a component of the P-TEFb complex that is important for transcription elongation, was the top hit in the screen and had the largest effect on HIV latency reversal with a wide variety of latency reversal agents. …”
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First record of Inosperma subsphaerosporum (Inocybaceae) in Indonesia by Иван П. Путра, Р. Риффиани, О. Д. Нурхайят, А. И. Тоемон

“…The BLAST result revealed that our specimen exhibits 96 % similarity to I. subsphaerosporum as the top hit. The phylogenetic tree (RAxML) nested our specimens in the I. subsphaerosporumclade from type material. …”
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Generation of a Transcriptome in a Model Lepidopteran Pest, Heliothis virescens, Using Multiple Sequencing Strategies for Profiling Midgut Gene Expression. by Omaththage P Perera, Kent S Shelby, Holly J R Popham, Fred Gould, Michael J Adang, Juan Luis Jurat-Fuentes

“…Of these, 29,978 had significant BLAST scores indicating orthologous relationships to transcripts of other insect species, with the top-hit species being the monarch butterfly (Danaus plexippus) and silkworm (Bombyx mori). …”
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Draft genome of multiple resistance donor plant Sinapis alba: An insight into SSRs, annotations and phylogenetics. by Preetesh Kumari, Kaushal Pratap Singh, Pramod Kumar Rai

“…The assembly was BLASTX with Arabidopsis thaliana which showed 32.9% positive hits between both plants. The top hit species distribution analysis showed the highest similarity with A. thaliana. …”
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An RNAi screen unravels the complexities of Rho GTPase networks in skin morphogenesis by Melanie Laurin, Nicholas C Gomez, John Levorse, Ataman Sendoel, Megan Sribour, Elaine Fuchs

“…Our screen unveiled hitherto unrecognized roles for Rho-mediated cytoskeletal remodeling events that impact hair follicle specification, differentiation, downgrowth and planar cell polarity. Coupling our top hit with gain/loss-of-function genetics, interactome proteomics and tissue imaging, we show that RHOU, an atypical Rho, governs the cytoskeletal-junction dynamics that establish columnar shape and planar cell polarity in epidermal progenitors. …”
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Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional programs. by Lipin Loo, Matthew A Waller, Cesar L Moreno, Alexander J Cole, Alberto Ospina Stella, Oltin-Tiberiu Pop, Ann-Kristin Jochum, Omar Hasan Ali, Christopher E Denes, Zina Hamoudi, Felicity Chung, Anupriya Aggarwal, Jason K K Low, Karishma Patel, Rezwan Siddiquee, Taeyoung Kang, Suresh Mathivanan, Joel P Mackay, Wolfram Jochum, Lukas Flatz, Daniel Hesselson, Stuart Turville, G Gregory Neely

“…Here, we use whole-genome CRISPR activation to identify host factors controlling cellular interactions with SARS-CoV-2. Our top hit was a TLR-related cell surface receptor called leucine-rich repeat-containing protein 15 (LRRC15). …”
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Exploring Aurone Derivatives as Potential Human Pancreatic Lipase Inhibitors through Molecular Docking and Molecular Dynamics Simulations by Phuong Thuy Viet Nguyen, Han Ai Huynh, Dat Van Truong, Thanh-Dao Tran, Cam-Van Thi Vo

“…Of them, 62 compounds interacted with the key residues of catalytic trial Ser152-Asp176-His263. The top hit compound (<b>A14</b>), with a docking score of −10.6 kcal⋅mol⁻¹, was subsequently submitted to molecular dynamics simulations, using GROMACS 2018.01. …”
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Tissue effect on genetic control of transcript isoform variation. by Tony Kwan, Elin Grundberg, Vonda Koka, Bing Ge, Kevin C L Lam, Christel Dias, Andreas Kindmark, Hans Mallmin, Osten Ljunggren, Fernando Rivadeneira, Karol Estrada, Joyce B van Meurs, Andre Uitterlinden, Magnus Karlsson, Claes Ohlsson, Dan Mellström, Olle Nilsson, Tomi Pastinen, Jacek Majewski

“…Examination of the top SNPs from a GWAS of bone mineral density show overlap with probeset associations observed in this study. The top hit corresponding to the FAM118A gene was tested for association studies in two additional clinical studies, revealing a novel transcript isoform variant. …”
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RNA-seq analysis of transcriptome and glucosinolate metabolism in seeds and sprouts of broccoli (Brassica oleracea var. italic). by Jinjun Gao, Xinxin Yu, Fengming Ma, Jing Li

“…After de novo assembly and searching the assembled transcripts against the Arabidopsis thaliana and NCBI nr databases, 19,441 top-hit transcripts were clustered as unigenes with an average length of 2,133 bp. …”
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Maximizing the reliability and the number of species assignments in metabarcoding studies using a curated regional library and a public repository by Audrey Bourret, Claude Nozères, Eric Parent, Geneviève J. Parent

“…With NCBI-nt and sequences from the regional library, the BLAST-LCA (least common ancestor) method was the most precise method for species assignments, but the accuracy was higher with the BLAST-TopHit method (&amp;gt;80% over all taxa, between 70% and 90% amongst taxonomic groups). …”
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Genome-wide analyses reveal the contribution of somatic variants to the immune landscape of multiple cancer types. by Wenjian Bi, Zhiyu Xu, Feng Liu, Zhi Xie, Hao Liu, Xiaotian Zhu, Wenge Zhong, Peipei Zhang, Xing Tang

“…Out of the key genes that drive multiple immune traits, top hit KEAP1 in lung adenocarcinoma (LUAD) was selected for validation. …”
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