In-Situ Observation of New Extra-Vascular Threadlike Structure of Mouse Using a Fluorescence Stereoscopic Microscope by Baeckkyoung Sung, Jawoong Lee, Byung-Cheon Lee, Hyun-Min Johng, Ku-Youn Baik, Tae Jeong Nam, Dae-Hun Park, Kyeong-Sun Soh, Kwang-Sup Soh

“…For this, we developed an in-situ searching method of the structure by vital staining with the dye of acridine orange and using a fluorescent stereomicroscope designed specifically for this purpose. …”
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Adjunctive aids for the detection of oral squamous cell carcinoma and oral potentially malignant disorders: A systematic review of systematic reviews by Jeremy Lau, Guru O, Saman Warnakulasuriya, Ramesh Balasubramaniam, Agnieszka Frydrych, Omar Kujan

“…This review found limited evidence to recommend chemiluminescence, tissue autofluorescence tools and vital staining as diagnostic tools, but only serve as clinical adjuncts to conventional oral examination. …”
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Nerve net differentiation in medusa development of Podocoryne carnea by Hans Gröger, Volker Schmid

“…Nerve nets of both polyps and developing medusae were examined in whole mounts and gelatin sections by using antibodies and vital staining with reduced Methylene Blue. In the polyp, both RFamide-positive nerve cells and tyrosine-tubulin containing nerve cells form an ectodermal plexus. …”
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Salivary and Lacrimal Gland Alterations of the Epidermal Fatty Acid-Binding Protein (E-FABP) in Non-Obese Diabetic Mice by Murat Dogru, Takashi Kojima, Cem Simsek, Taeko Nagata, Kazuo Tsubota

“…Cotton thread and ocular vital staining tests were performed on 10-week NOD male mice (<i>n</i> = 24) and age- and sex-matched wild-type (WT) mice (<i>n</i> = 25). …”
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Inactivation of Giardia intestinalis cysts in water using a sonochemical method based on mid-high-frequency waves by Laura María Gómez-Quintero, Marlon Alexis Múnera-Marín, María Alejandra Urán-Serna, Efraím A. Serna-Galvis, Ana Luz Galván-Diaz, Ricardo A. Torres-Palma

“…The viability of the protozoan cysts was evaluated using the immunofluorescence technique and vital stains, showing this protocol was useful to quantify the parasite. …”
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"Scolicidal effect of alcoholic, aqueous and total alkaloids of Peganum Harmala L. (Syrian Rue) against hydatid cysts protoscolices " by Mahdavi M, Masood J

“…Mortality of protoscolices was determined by their loss of mortality, ability to take vital stains and inactivity of flame cells. Results: The results indicated that alcoholic extract had scolicidal effect against protoscolices; total alkaloids had stronger action and rapid effect on protoscolices.…”
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Mitomycin-C Treatment Followed by Culture Produces Long-Term Survival of Islet Xenografts in a Rat-to Mouse Model by Takashi Gunji, Takuro Saito, Yoshihiro Sato, Shinichi Matsuyama, Kazuya Ise, Takashi Kimura, Takayuki Anazawa, Mitsukazu Gotoh M.D.

“…Evaluation of in vitro insulin secretory capacity and vital staining of islets indicated that MMC at a dose ≤32 μg/ml is nontoxic and preserves islet function. …”
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Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins. by Miesenböck, G, Rothman, J

“…Photon emissions required a depolarizing stimulus, occurred from regions with high synaptic density as ascertained by vital staining of recycling synaptic vesicles, and were sensitive to Ca2+ depletion and clostridial neurotoxins. …”

Cellular migration, transition and interaction during regeneration of the sponge Hymeniacidon heliophila. by Cristiano C Coutinho, Ivone de Andrade Rosa, John Douglas de Oliveira Teixeira, Leonardo R Andrade, Manoel Luis Costa, Claudia Mermelstein

“…An innovative sponge organotypic culture of the regenerating internal region is described and investigated by confocal microscopy, cell transplantation and vital staining. Cell-cell interaction and cell density are shown to affect events in morphogenesis such as epithelial/mesenchymal and mesenchymal/epithelial transitions as well as distinct cell movements required for regeneration. …”
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Suppressive effects of the anti-oxidant N-acetylcysteine on the anti-malarial activity of artesunate. by Arreesrisom, P, Dondorp, A, Looareesuwan, S, Udomsangpetch, R

“…Assessment of parasite maturation inhibition by the standard Giemsa's staining was in accordance with the use of a vital staining. The results herein caution the use of adjunctive treatment for malaria infection. …”

Real-Time Monitoring of Lysosomal Membrane Permeabilization Using Acridine Orange by Ida Eriksson, Linda Vainikka, Hans Lennart Persson, Karin Öllinger

“…The weak base acridine orange readily enters cells and accumulates in the acidic environment of lysosomes. Vital staining with acridine orange is a well-proven technique to observe lysosomal destabilization using fluorescence microscopy and flow cytometry. …”
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Noninvasive Imaging Methods to Improve the Diagnosis of Oral Carcinoma and Its Precursors: State of the Art and Proposal of a Three-Step Diagnostic Process by Antonio Romano, Dario Di Stasio, Massimo Petruzzi, Fausto Fiori, Carlo Lajolo, Andrea Santarelli, Alberta Lucchese, Rosario Serpico, Maria Contaldo

“…The present review aims to focus on the most consolidated diagnostic techniques, such as vital staining and tissue autofluorescence, and to report the potential role of some of the most promising innovative techniques, such as narrow-band imaging, high-frequency ultrasounds, optical coherence tomography, and in vivo confocal microscopy. …”
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Individual marking of soft-bodied subtidal invertebrates in situ - A novel staining technique applied to the giant plumose anemone Metridium farcimen (Tilesius, 1809). by Christopher D Wells, Kenneth P Sebens

“…We tested, in laboratory and field studies, a new way of marking sea anemones (order Actiniaria) by injection of three vital stains (i.e., neutral red, methylene blue, and fluorescein). …”
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Control by arbuscular endomycorrhizae of Pratylenchus brachyurus in pineapple microplants by Jean-Philippe Guillemin, Silvio Gianinazzi, Vivienne Gianinazzi-Pearson, Jean Marchal

“…Nematode inoculation affected endomycorrhizal colonization estimated by non vital staining for the Queen Tahiti and Spanish varieties but did not alter development of metabolically active arbuscules in roots of the three varieties. …”
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Regional Metabolic Disturbances and Cerebrovascular Anatomy after Permanent Middle Cerebral Artery Occlusion in C57Black/6 and SV129 Mice by K. Maeda, R. Hata, K.-A. Hossmann

“…The vascular anatomy was studied by latex infusion, brain infarction by vital staining, the size of the ischemic penumbra by imaging of ATP and protein synthesis, and blood flow by laser–Doppler flowmetry. …”
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Nanotechnology: a promising method for oral cancer detection and diagnosis by Xiao-Jie Chen, Xue-Qiong Zhang, Qi Liu, Jing Zhang, Gang Zhou

“…The application of traditional noninvasive methods-such as vital staining, exfoliative cytology, and molecular imaging-is limited by insufficient sensitivity and specificity. …”
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Evaluation of the Protoscolicidal Effectiveness of Hypertonic Saline, Silver Nitrate, Ethanol, Using Sponge Pad Method and Injecting into Fertile Hydatid Cysts by Kereshmeh Fakharzadeh Jahromi, Abdollah Rafiei, Mahmoud Rahdar, Amin Bahreini

“…Then, after different exposure times (5, 10, and 15 min), the protoscolices were aspirated from the cyst and viability of the protoscolices were evaluated microscopically using vital staining. In the second part, the live protoscolices were sprayed on the soaked sponge with the mentioned protoscolicidal agents. …”
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Polyphenol Composition of Extracts of the Fruits of <i>Laserpitium Krapffii</i> Crantz and Their Antioxidant and Cytotoxic Activity by Anna Bogucka-Kocka, Natalia Vorobets, Małgorzata Chrząszcz, Wioleta Pietrzak, Katarzyna Szewczyk

“…The cytotoxicity was estimated using trypan blue vital staining. It was found that the analyzed extracts induced the apoptosis of the cells of all the tested cell lines. …”
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FLOW-CYTOFLUOROMETRIC STUDY OF BACTERICIDAL GRANULES IN BLOOD PHAGOCYTES OF ANIMALS WITH VARIOUS SPECIES SENSITIVITY TO EXPERIMENTAL PLAGUE INFECTION by A. L Kravtsov

“…Acridine orange (AO) was used for supra-vital staining in primary (bactericidal) granule cells. …”
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Confluence of Cellular Degradation Pathways During Interdigital Tissue Remodeling in Embryonic Tetrapods by Juan A. Montero, Carlos I. Lorda-Diez, Juan M. Hurle

“…Our review proposes that cell senescence is the biological process identified by vital staining in embryonic models and implicates lysosomes in programmed cell death. …”
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