Development of Domestic Cat Embryo Produced by Preserved Sperms by KARTINI ERIANI, ARIEF BOEDIONO, ITA DJUWITA, SONY HERU SUMARSONO, AL-AZHAR4

“…The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. …”
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Effect of Taxus baccata L. Extract on Hydatid Cyst Protoscolices In vitro by R. Norouzi, M. Hejazy, D. Azizi, A. Ataei

“…Viability of protoscolices was confirmed by 0.1% eosin vital staining. The data were analyzed in SAS software (version 9.4). …”
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The correlation between urinary 5-hydroxyindoleacetic acid and sperm quality in infertile men and rotating shift workers by Pariente José A, García Juan F, Lozano Graciela M, Monllor Fabián, Bejarano Ignacio, Espino Javier, Ortiz Águeda, Rodríguez Ana B

“…Sperm morphology was evaluated after Diff-Quik staining, while sperm vitality was estimated after Eosin-Nigrosin vital staining.</p> <p>Results</p> <p>Our results show that urinary 5-HIIA levels obtained from a set of 20 volunteers negatively correlated with sperm concentration, forward motility, morphology normal range and sperm vitality. …”
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Immediate post-interventional contrast-enhanced computed tomography overestimates hepatic microwave ablation – an in vivo animal study by Franz G. Poch, Beatrice Geyer, Ole Gemeinhardt, Robert Klopfleisch, Stefan M. Niehues, Janis L. Vahldiek, Keno Bressem, Martin E. Kreis, Kai S. Lehmann

“…Samples were histologically analyzed after vital staining (NADH-diaphorase). Ablation zones were histologically and macroscopically outlined. …”
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Genome resource banking in the family Felidae by S. Ya. Amstislavsky, V. I. Mokrousova, V. V. Kozhevnikova, E. A. Kizilova, E. Yu. Brusentsev, K. A. Okotrub, V. A. Naprimerov, S. V. Naidenko

“…Different methods of frozen-thawed spermatozoa and embryonic viability testing were used in this study, including vital staining and subsequent fluorescent and light microscopy, and heterologous in vitro fertilization.…”
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The Effect of High Molecular Weight Hyaluronic Acid and Latanoprost Eyedrops on Tear Functions and Ocular Surface Status in C57/BL6 Mice by Murat Dogru, Takashi Kojima, Kazunari Higa, Ayako Igarashi, Haruka Kudo, Wolfgang G. K. Müller-Lierheim, Kazuo Tsubota, Kazuno Negishi

“…In conclusion, the new CS + LP formulation appeared to induce less inflammation, less corneal vital staining, and a better barrier status with an IOP lowering effect comparable to the commercial LP eye drops.…”
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Comparison of corneal thickness in patients with dry eye disease using the Pentacam rotating Scheimpflug camera and anterior segment optical coherence tomography. by Keiichi Fujimoto, Takenori Inomata, Yuichi Okumura, Nanami Iwata, Kenta Fujio, Atsuko Eguchi, Ken Nagino, Hurramhon Shokirova, Maria Karasawa, Akira Murakami

“…DED was diagnosed using the Asia Dry Eye Society criteria and further divided into mild and severe DED based on kerato-conjunctival vital staining. Central corneal thickness, thinnest corneal thickness, and the thinnest point of the cornea measured by Pentacam and ASOCT were compared, and Pearson's correlation coefficients were estimated. …”
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Phenolic Composition of the Leaves of <i>Pyrola rotundifolia</i> L. and Their Antioxidant and Cytotoxic Activity by Katarzyna Szewczyk, Anna Bogucka-Kocka, Natalia Vorobets, Anna Grzywa-Celińska, Sebastian Granica

“…The cytotoxic effect in increasing concentration on five types of leukemic cell lines was also investigated using trypan blue vital staining. It was found that the analyzed extract induced the apoptosis of all the tested cell lines. …”
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Radiofrequency and microwave ablation in a porcine liver model: non-contrast CT and ultrasound radiologic-pathologic correlation by Timothy J. Ziemlewicz, J. Louis Hinshaw, Meghan G. Lubner, Emily A. Knott, Bridgett J. Willey, Fred T. Lee, Christopher L. Brace

“…Each ablation was sectioned in the plane of the ultrasound image and underwent vital staining to delineate cellular necrosis. CT was reformatted to the same plane as the ultrasound transducer and transverse diameters of gas and hypoechoic/hypoattenuating zones at each time point were measured. …”
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Midgut barrier imparts selective resistance to filarial worm infection in Culex pipiens pipiens. by Michelle L Michalski, Sara M Erickson, Lyric C Bartholomay, Bruce M Christensen

“…Examination of parasites recovered from the Cx. p. pipiens midgut by vital staining, and those exsheathed with papain, suggest that the damage inflicted by the midgut is subcuticular and disrupts internal tissues. …”
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The Influence of a Metal Stent on the Distribution of Thermal Energy during Irreversible Electroporation. by Hester J Scheffer, Jantien A Vogel, Willemien van den Bos, Robert E Neal, Krijn P van Lienden, Marc G H Besselink, Martin J C van Gemert, Cees W M van der Geld, Martijn R Meijerink, John H Klaessens, Rudolf M Verdaasdonk

“…Tissue viability after in-vivo IRE was investigated macroscopically using 5-triphenyltetrazolium chloride (TTC) vitality staining. RESULTS:In the gel, direct stent-heating was not observed. …”
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Antiplaque effect of essential oils and 0.2% chlorhexidine on an in situ model of oral biofilm growth: a randomised clinical trial. by Víctor Quintas, Isabel Prada-López, Nikolaos Donos, David Suárez-Quintanilla, Inmaculada Tomás

“…Posteriorly, after bacterial vital staining, samples were analysed using a Confocal Laser Scanning Microscope. …”
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Bioerosion and fungal colonization of the invasive foraminiferan <i>Amphistegina lobifera</i> in a Mediterranean seagrass meadow by M. Vohník

“…However, little is known about the turnover of its shells in the invaded ecosystems. Using vital staining, stereomicroscopy, scanning electron microscopy, and cultivation and DNA fingerprinting, I investigated the vital status, destruction/decomposition and mycobiota of <i>A. lobifera</i> in the rhizosphere of the dominant Mediterranean seagrass <i>Posidonia oceanica</i> in an underwater Maltese meadow (average 284 shells g<span class="inline-formula">⁻¹</span>, representing 28.5 % of dry substrate weight), in comparison with epiphytic specimens and <i>P. oceanica</i> roots. …”
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Significant biochemical, biophysical and metabolic diversity in circulating human cord blood reticulocytes. by Benoît Malleret, Fenggao Xu, Narla Mohandas, Rossarin Suwanarusk, Cindy Chu, Juliana A Leite, Kayen Low, Claudia Turner, Kanlaya Sriprawat, Rou Zhang, Olivier Bertrand, Yves Colin, Fabio T M Costa, Choon Nam Ong, Mah Lee Ng, Chwee Teck Lim, Francois Nosten, Laurent Rénia, Bruce Russell

“…While reticulocyte staging based on fluorescent RNA stains such as thiazole orange have been useful in a clinical setting; these 'sub-vital' stains may confound delicate studies on reticulocyte biology and may preclude their use in heamoparasite invasion studies. …”
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Comparison of diagnostic tests in distinct well-defined conditions related to dry eye disease. by Monica Alves, Peter Sol Reinach, Jayter Silva Paula, Antonio Augusto Vellasco e Cruz, Leticia Bachette, Jacqueline Faustino, Francisco Penteado Aranha, Afonso Vigorito, Carmino Antonio de Souza, Eduardo Melani Rocha

“…CONCLUSIONS: DED diagnostic test results present a broad range of variability among different conditions. Vital stainings and TBUT correlated best with one another whereas the best test combination to detect DED was: OSDI/TBUT/Schirmer.…”
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Lysosomes, caspase-mediated apoptosis, and cytoplasmic activation of P21, but not cell senescence, participate in a redundant fashion in embryonic morphogenetic cell death by Cristina Duarte-Olivenza, Goretti Moran, Juan M. Hurle, Carlos I. Lorda-Diez, Juan A. Montero

“…In the assay, “naive” progenitors obtained from the autopod aggregate to form chondrogenic nodules and those occupying the internodular spaces exhibit intense apoptosis and progressive accumulation of larger cells, showing intense SA-β-Gal histochemical labelling that strictly overlaps with the distribution of neutral red vital staining. qPCR analysis detected intense upregulation of the p21 gene, but P21 immunolabelling showed cytoplasmic rather than the nuclear distribution expected in senescent cells. …”
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Comparing the efficacy of chemiluminescence with lugol's iodine versus toluidine blue in the diagnosis of dysplasia in tobacco associated oral lesions - A diagnostic study by K V Swathi, G Maragathavalli, T N Uma Maheswari

“…Because it is a non-invasive procedure, it can be used to evaluate dysplastic changes in various OPMDs at their initial stage for timely intervention. Vital staining has also been used as a chair-side marker for delineating normal and dysplastic oral mucosal tissues. …”
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Markers of platelet activation and apoptosis during storage of apheresis- and buffy coat-derived platelet concentrates for 7 days. by Albanyan, A, Harrison, P, Murphy, M

“…PLT viability was measured using a new assay that utilizes the vital stains calcein-AM and FM4-64. Expression of phosphatidylserine (PS), CD42b, CD47, CD61, and CD62P was also assessed. …”

Significant biochemical, biophysical and metabolic diversity in circulating human cord blood reticulocytes by Malleret, B, Xu, F, Mohandas, N, Suwanarusk, R, Chu, C, Leite, J, Low, K, Turner, C, Sriprawat, K, Zhang, R, Bertrand, O, Colin, Y, Costa, F, Ong, C, Ng, M, Lim, C, Nosten, F, Rénia, L, Russell, B

“…While reticulocyte staging based on fluorescent RNA stains such as thiazole orange have been useful in a clinical setting; these ‘sub-vital’ stains may confound delicate studies on reticulocyte biology and may preclude their use in heamoparasite invasion studies. …”

Perivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model by F. G. M. Poch, C. A. Neizert, B. Geyer, O. Gemeinhardt, S. M. Niehues, J. L. Vahldiek, K. K. Bressem, K. S. Lehmann

“…Three experimental settings were planned: (1) inter-applicator-distance: 15 mm; (2) inter-applicator-distance: 20 mm; (3) inter-applicator-distance: 20 mm with hepatic inflow occlusion (Pringle maneuver). A vitality staining was used to analyze liver cell vitality around all vessels in the ablation center with a diameter > 0.5 mm histologically. 771 vessels were identified. …”
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