The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (<i>Sod1</i>) Knockout Mice Treated with Antiglaucoma Eye Med... by Yukari Yagi-Yaguchi, Takashi Kojima, Kazunari Higa, Murat Dogru, Osama MA. Ibrahim, Takahiko Shimizu, Kazuo Tsubota, Jun Shimazaki

“…Mice underwent assessments of tear quantity, tear film breakup time, and vital staining score. Mice in groups 3 and 4 showed significantly better tear stability and lower corneal staining scores than mice in group 2 after eye drop instillations (<i>p</i> &lt; 0.05). …”
Get full text

Pomegranate Peel Extract Is a Potential Alternative Therapeutic for Giardiasis by Asmaa M. El-Kady, Iman A. M. Abdel-Rahman, Samer S. Fouad, Khaled S. Allemailem, Taghrid Istivan, Sheren F. M. Ahmed, Al Shaimaa Hasan, Heba A. Osman, Hatem A. Elshabrawy

“…In vitro study showed high efficacy of pomegranate peel ethanolic extract in killing <i>G. lamblia</i> cysts as demonstrated by eosin vital staining. We showed that treating infected rats with pomegranate extract resulted in a marked reduction in the mean number of <i>G. lamblia</i> cysts and trophozoites in feces and intestine respectively. …”
Get full text

Oral White Lesions Associated with Chewing Khat by Meir Gorsky, Joel B. Epstein, Harel Levi, Noam Yarom

“…Even though the majority of the white lesions (85.4%) were homogenous, 71.4% of the non homogenous lesions were identified in khat chewers. Vital staining with toluidine blue and exfoliative cytology was conducted on a subset of patients with homogenous and non-homogenous oral lesions, and there were no findings suspicious for pre-malignant or malignant changes. …”
Get full text

The synthetic inhibitor of Fibroblast Growth Factor Receptor PD166866 controls negatively the growth of tumor cells in culture by Castelli Mauro, Ciacciarelli Marina, Risuleo Gianfranco, Galati Gaspare

“…Cytoxicity was assessed by the Mosmann assay and by vital staining with trypan blue. The target of the molecule is most likely the cell membrane as shown by the significant increase of the intracellular concentration of malonyl-dihaldheyde. …”
Get full text

A dose-escalation ex vivo study on the effects of intracameral benzalkonium chloride in rabbits by Sergi Segarra, Marta Leiva, Daniel Costa, Natàlia Coyo, Maria Sabés-Alsina, José Ríos, Teresa Peña

“…At 0, 6, 24 and 48 h, ophthalmologic examination of the anterior segment, pachymetry and specular microscopy were performed, and corneas were finally vital-stained and observed under the light microscope to assess the CECs morphology and mortality rate. …”
Get full text

Development of Domestic Cat Embryo Produced by Preserved Sperms by KARTINI ERIANI, ARIEF BOEDIONO, ITA DJUWITA, SONY HERU SUMARSONO, AL-AZHAR

“…The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. …”
Get full text

Confocal Endomicroscopy of Neuromuscular Junctions Stained with Physiologically Inert Protein Fragments of Tetanus Toxin by Cornelia Roesl, Elizabeth R. Evans, Kosala N. Dissanayake, Veronika Boczonadi, Ross A. Jones, Graeme R. Jordan, Leire Ledahawsky, Guy C. C. Allen, Molly Scott, Alanna Thomson, Thomas M. Wishart, David I. Hughes, Richard J. Mead, Clifford C. Shone, Clarke R. Slater, Thomas H. Gillingwater, Paul A. Skehel, Richard R. Ribchester

“…Live imaging of neuromuscular junctions (NMJs) in situ has been constrained by the suitability of ligands for inert vital staining of motor nerve terminals. Here, we constructed several truncated derivatives of the tetanus toxin C-fragment (TetC) fused with Emerald Fluorescent Protein (emGFP). …”
Get full text

A technique for separating viable islets of Langerhans from a fragment of human pancreatic tail by A. S. Ponomareva, L. A. Kirsanova, N. V. Baranova, G. N. Bubentsova, I. A. Miloserdov, E. A. Volkova, V. I. Sevastyanov

“…The morphological study of the EP fragments at different times of cold ischemia did not reveal significant differences in the histological presentation of the organ parenchyma; the islet structure appeared intact. Vital staining confirmed the separated IL viability in vitro for at least 1–3 days.Conclusion. …”
Get full text

Trichoplax sp. H2 cultivation and regeneration from body fragments and dissociated cell aggregates: Outlook for genetic modification by A. V. Kuznetsov, V. I. Vainer, Yu. M. Volkova, V. M. Tsygankova, D. N. Bochko, V. S. Mukhanov

“…The animals after a vital staining were dissociated into single cells in a medium with no divalent cations. …”
Get full text

Selective retina therapy and thermal stimulation of the retina: different regenerative properties - implications for AMD therapy by Elisabeth Richert, Julia Papenkort, Claus von der Burchard, Alexa Klettner, Philipp Arnold, Ralph Lucius, Ralf Brinkmann, Carsten Framme, Johann Roider, Jan Tode

“…For morphological analysis, porcine RPE/choroid organ cultures underwent the same laser treatment and were examined by calcein vitality staining 1 h and 1, 3 or 5 days after irradiation. …”
Get full text

Acidification of the parasitophorous vacuole containing Toxoplasma gondii in the presence of hydroxyurea by Cristiane S. Carvalho, Edésio J.T. Melo

“…In the present work we investigated the cellular mechanism involved in the destruction of intracellular Toxoplasma gondii. Fluorescent vital stains were used in order to observe possible acidification of parasitophorous vacuole-containing Toxoplasma gondii in presence of hydroxyurea. …”
Get full text

Autophagy inhibition enhances RAD001-induced cytotoxicity in human bladder cancer cells by Lin JF, Lin YC, Yang SC, Tsai TF, Chen HE, Chou KY, Hwang TIS

“…Acidic vesicular organelle (AVO) formation in treated cells was determined by acridine orange vital staining. Inhibition of mTOR pathway by RAD001 was monitored by using a homemade quantitative polymerase chain reaction gene array, while phospho-mTOR was detected using Western blot. …”
Get full text

Experimental approaches to creating a tissue-specific matrix for a bioartificial liver by A. M. Grigoriev, Yu. B. Basok, A. D. Kirillova, L. A. Kirsanova, N. P. Shmerko, A. M. Subbot, E. A. Nemets, I. A. Miloserdov, M. Yu. Shagidulin, V. I. Sevastyanov

“…Viability and metabolic activity of cells were assessed via vital staining with a complex of fluorescent dyes LIVE/DEAD ® and PrestoBlue™ (Invitrogen, USA). …”
Get full text

Dexamethasone-(C21-phosphoramide)-[anti-EGFR]: molecular design, synthetic organic chemistry reactions, and antineoplastic cytotoxic potency against pulmonary adenocarcinoma (A549) by Coyne CP, Narayanan L

“…Following spectrophotometric analysis and validation of retained epidermal growth factor receptor type 1 (EGFR)-binding avidity by cell-ELISA, the selective anti-neoplasic cytotoxic potency of dexamethasone-(C21-phosphoramide)-[anti-EGFR] was established by MTT-based vitality stain methodology using adherent monolayer populations of human pulmonary adenocarcinoma (A549) known to overexpress the tropic membrane receptors EGFR and insulin-like growth factor receptor type 1. …”
Get full text

Corneal In Vivo Laser-Scanning Confocal Microscopy Findings in Dry Eye Patients with Sjögren’s Syndrome by Yukihiro Matsumoto, Osama M. A. Ibrahim, Takashi Kojima, Murat Dogru, Jun Shimazaki, Kazuo Tsubota

“…Results: The tear quantity, stability, and vital staining scores were significantly worse in patients with SS than in control subjects (<i>p</i> < 0.0001). …”
Get full text

Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm by Adriana Novaes dos Reis, Moysés dos Santos Miranda, Lílian Kátia Ximenes Silva, Aluizio Otavio Almeida da Silva, José Silva de Sousa, Eziquiel de Morais, Alexandre Rossetto Garcia, Sheyla Farhayldes Souza Domingues, Joyce Kelly do Rosário da Silva, Haroldo Francisco Lobato Ribeiro

“…After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. …”
Get full text

Persistent Urogenital Schistosomiasis and Its Associated Morbidity in Endemic Communities within Southern Ghana: Suspected Praziquantel<i> </i>Resistance or Reinfection? by Patience B. Tetteh-Quarcoo, Peter O. Forson, Seth K. Amponsah, John Ahenkorah, Japheth A. Opintan, Janet E. Y. Ocloo, Esther N. Okine, Robert Aryee, Emmanuel Afutu, Abraham K. Anang, Patrick F. Ayeh-Kumi

“…Viability was assessed using a modified hatchability technique, vital staining (0.4% trypan blue and 1% neutral red) and fluorescent (Hoechst 33258) microscopy. …”
Get full text

RECOMMENDED TACTICS FOR THE EVALUATION OF POTENTIALLY MALIGNANT DISORDERS IN THE ORAL CAVITY by S.V. Kolomiiets, K.O. Udaltsova, V.I. Shynkevych

“…Adjunctive test for precancerous and cancerous cases in the oral cavity (include autofluorescence, tissue reflectance, vital staining, and salivary adjuncts) is recognized by the Ukrainian and American experts, as “adjuncts”, none of which was felt to be accurate enough to identify target lesions in the primary care setting. …”
Get full text

EVALUATION OF CARIOGENIC SITUATION IN CHILDREN WITH TYPE 1 DIABETES MELLITUS GIVEN THE MINERALIZING POTENTIAL OF SALIVA AND ENAMEL RESISTANCE by I. M. BYKOV, F. N. GILMIYAROVA, D. A. DOMENYUK, S. V. DMITRIENKO, S. O. IVANYUTA, G. M.-A. BUDAYCHIEV

“…Kosavera, 1984)  and vital staining (L.A. Aksamit, 1978). The electrometry of  hard tooth tissues was carried out by the electrodiagnostic apparatus "Dent Est" (V.K. …”
Get full text

Dry Eye Epidemiology in Patients before Cataract Surgery by S. N. Sakhnov, S. V. Yanchenko, A. V. Malyshev, V. V. Dashina, A. R. Ceeva, L. M. Petrosyan

“…OSDI testing, lipid interferential test, TBUT, Shirmer-1, -2 tests, visual and OCT meniscometry, evaluation of epitheliopaty and microerosion (with vital staining), compression Norn test in Korb modification, visual and OCT LIPCOF assessment, lid viper epitheliopathy evaluation, anterior segment of the eye photoregistration with computer morphometry were performed to assess the ocular surface condition. …”
Get full text