Coupling the Cardiac Voltage-Gated Sodium Channel to Channelrhodopsin-2 Generates Novel Optical Switches for Action Potential Studies by Christian vom Dahl, Christoph Emanuel Müller, Xhevat Berisha, Georg Nagel, Thomas Zimmer

“…Fusion constructs were expressed in <i>Xenopus laevis</i> oocytes, and electrophysiological recordings were performed by the two-microelectrode technique. …”
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GJA1 depletion causes ciliary defects by affecting Rab11 trafficking to the ciliary base by Dong Gil Jang, Keun Yeong Kwon, Yeong Cheon Kweon, Byung-gyu Kim, Kyungjae Myung, Hyun-Shik Lee, Chan Young Park, Taejoon Kwon, Tae Joo Park

“…Here, we examined the functions of GJA1 during ciliogenesis in human retinal pigment epithelium-1 and Xenopus laevis embryonic multiciliated-cells. GJA1 localizes to the motile ciliary axonemes or pericentriolar regions beneath the primary cilium. …”
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Identification of novel genes including NAV2 associated with isolated tall stature by Birgit Weiss, Tim Ott, Philipp Vick, Julian C. Lui, Ralph Roeth, Sebastian Vogel, Stephan Waldmüller, Sandra Hoffmann, Jeffrey Baron, Jan M. Wit, Gudrun A. Rappold

“…Knockout of the all-trans retinoic acid responsive gene, neuron navigator 2 NAV2, in Xenopus supports its functional role as a growth promotor. …”
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Snapin Specifically Up-Regulates Ca_v1.3 Ca²⁺ Channel Variant with a Long Carboxyl Terminus by Sua Jeong, Jeong-Seop Rhee, Jung-Ha Lee

“…Co-expression of Snapin with Ca_v1.3_L/Ca_vβ₃/α₂δ₂ subunits increased the peak current density or amplitude by about 2-fold in HEK-293 cells and <i>Xenopus</i> oocytes, without affecting voltage-dependent gating properties and calcium-dependent inactivation. …”
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Evidence for a Genotype–Phenotype Correlation in Patients with Pathogenic <i>GLUT2</i> (<i>SLC2A2</i>) Variants by Sarah C. Grünert, Anke Schumann, Federico Baronio, Konstantinos Tsiakas, Simona Murko, Ute Spiekerkoetter, René Santer

“…This course was correlated to data from old and most recent expression and transport studies in <i>Xenopus</i> oocytes. <i>GLUT2</i> genotype in patients 1 and 2 was p.[153_4delLI];[P417R] with the first variant exhibiting normal membrane expression and partially retained transport activity (5.8%) for 2-deoxyglucose. …”
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Antibacterial and In Vivo Studies of a Green, One-Pot Preparation of Copper/Zinc Oxide Nanoparticle-Coated Bandages by Archana R. Deokar, Ilana Perelshtein, Melissa Saibene, Nina Perkas, Paride Mantecca, Yeshayahu Nitzan, Aharon Gedanken

“…Nanotoxicology investigations were conducted via in vivo examinations of the effect of the metal-oxide bandages on frog embryos (teratogenesis assay—<i>Xenopus</i>). The results show that water-based coatings resulted in lesser impacts on embryo development than the ethanol-based ones. …”
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Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signaling by Woong Y Hwang, Valentyna Kostiuk, Delfina P González, C Patrick Lusk, Mustafa K Khokha

“…We further demonstrate direct binding between TNPO1 and β-catenin that is mediated by a conserved PY-NLS. Finally, using Xenopus secondary axis and TCF/LEF (T Cell factor/lymphoid enhancer factor family) reporter assays, we demonstrate that our results in yeast can be directly translated to vertebrates. …”
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A cyclin-dependent kinase-mediated phosphorylation switch of disordered protein condensation by Juan Manuel Valverde, Geronimo Dubra, Michael Phillips, Austin Haider, Carlos Elena-Real, Aurélie Fournet, Emile Alghoul, Dhanvantri Chahar, Nuria Andrés-Sanchez, Matteo Paloni, Pau Bernadó, Guido van Mierlo, Michiel Vermeulen, Henk van den Toorn, Albert J. R. Heck, Angelos Constantinou, Alessandro Barducci, Kingshuk Ghosh, Nathalie Sibille, Puck Knipscheer, Liliana Krasinska, Daniel Fisher, Maarten Altelaar

“…To understand how this complexity produces an ordered and rapid cellular reorganisation, we generated a high-resolution map of changing phosphosites throughout unperturbed early cell cycles in single Xenopus embryos, derived the emergent principles through systems biology analysis, and tested them by biophysical modelling and biochemical experiments. …”
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Regulatory consequences of mRNA Poly(A)-Tail length changes by Eichhorn, Stephen W. (Stephen William)

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Function and regulation of poly(A)-tail length by Subtelny, Alexander O. (Alexander Orest)

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Single-hit inactivation drove tumor suppressor genes out of the X chromosome during evolution by Wang, Xiansong, Hu, Wei, Li, Xiangchun, Huang, Dan, Li, Qing, Chan, Hung, Zeng, Judeng, Xie, Chuan, Chen, Huarong, Liu, Xiaodong, Gin, Tony, Wang, Maggie Haitian, Cheng, Alfred Sze Lok, Kang, Wei, To, Ka-Fai, Plewczynski, Dariusz, Zhang, Qingpeng, Chen, Xiaoting, Chan, Danny Cheuk Wing, Ko, Ho, Wong, Sunny Hei, Yu, Jun, Chan, Matthew Tak Vai, Zhang, Lin, Wu, William Ka Kei

“…This was verified in other mammals by assessing the concordance/discordance of chromosomal locations of mammalian TSGs and their orthologs in Xenopus tropicalis. In humans, X-linked TSGs are younger or larger in size. …”
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Disease progression and search for monogenic diabetes among children with new onset type 1 diabetes negative for ICA, GAD- and IA-2 Antibodies by Pörksen, S, Laborie, L, Nielsen, L, Louise Max Andersen, M, Sandal, T, De Wet, H, Schwarcz, E, Aman, J, Swift, P, Kocova, M, Schönle, E, de Beaufort, C, Hougaard, P, Ashcroft, F, Molven, A, Knip, M, Mortensen, H, Hansen, L, Njølstad, P

“…We expressed recombinant K-ATP channels in Xenopus oocytes to analyse the functional effects of an ABCC8 mutation.…”

Involvement of the n-terminus of Kir6.2 in coupling to the sulphonylurea receptor. by Reimann, F, Tucker, S, Proks, P, Ashcroft, F

“…We examined the effect of N-terminal deletions of Kir6.2 on the channel open probability, ATP sensitivity and sulphonylurea sensitivity by recording macroscopic currents in membrane patches excised from Xenopus oocytes expressing wild-type or mutant Kir6.2/SUR1. 3. …”

Altered functional properties of KATP channel conferred by a novel splice variant of SUR1. by Sakura, H, Trapp, S, Liss, B, Ashcroft, F

“…We examined the properties of KATP channels composed of Kir6.2 and SUR1bDelta33 by recording macroscopic currents in membrane patches excised from Xenopus oocytes expressing these subunits. We also investigated the effect of truncating SUR1 at either the start (SUR1bT1) or end (SUR1bT2) of exon 33 on KATP channel properties. 3. …”

Differential Regulation of Human Serotonin Receptor Type 3A by Chanoclavine and Ergonovine by Sanung Eom, Woog Jung, Jaeeun Lee, Hye Duck Yeom, Shinhui Lee, Chaelin Kim, Heui-Dong Park, Junho H. Lee

“…Electrophysiological experiments were conducted using a two-electrode voltage-clamp analysis to observe the inhibitory effects of chanoclavine on <i>Xenopus</i> oocytes in which the h5-HT_3A receptor was expressed. …”
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Virus-Targeted Transcriptomic Analyses Implicate Ranaviral Interaction with Host Interferon Response in Frog Virus 3-Infected Frog Tissues by Yun Tian, Francisco De Jesús Andino, Collins N. Khwatenge, Jiuyi Li, Jacques Robert, Yongming Sang

“…To establish a high-throughput characterization of ranaviral gene expression at the genome scale, we performed a whole transcriptomic analysis (RNA-Seq) using total RNA samples containing both viral and cellular transcripts from FV3-infected <i>Xenopus laevis</i> adult tissues using two FV3 strains, a wild type (FV3-WT) and an ORF64R-deleted recombinant (FV3-∆64R). …”
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Effects of JZTX-V on the wild type Kv4.3 Expressed in HEK293T and Molecular Determinants in the Voltage-sensing Domains of Kv4.3 Interacting with JZTX-V by Xu Dehong, Wu Wenmei, Hong Siqin, Zeng Peng, Wang Xianchun, Zeng Xiongzhi

“…Previous studies had shown that JZTX-V could inhibit the transient outward potassium current of Kv4.2 and Kv4.3 expressed in Xenopus oocytes but had no effects on Kv1.2–1.4. However, the underlying action mechanism of JZTX-V on Kv4.3 remains unclear. …”
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α-Conotoxin as Potential to α7-nAChR Recombinant Expressed in <i>Escherichia coli</i> by Yanli Liu, Yifeng Yin, Yunyang Song, Kang Wang, Fanghui Wu, Hui Jiang

“…Bioactivity of rArIB and rArIB (V11L, V16A) was assessed by two-electrode voltage-clamp electrophysiology in <i>Xenopus laevis oocytes</i> expressing human nAChR subtypes. …”
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Fluorescently Labeled α-Conotoxin TxID, a New Probe for α3β4 Neuronal Nicotinic Acetylcholine Receptors by Meiling Huang, Xiaopeng Zhu, Yishuai Yang, Yao Tan, Sulan Luo, Dongting Zhangsun

“…Additionally, the potency and selectivity of TxID-F were determined by using a two-electrode voltage-clamp technique on various nAChRs expressed in the <i>Xenopus</i> oocyte expression system. The results obtained by electrophysiology showed that TxID-F maintained the same order of potency (IC₅₀ 73 nM) as the native toxin (IC₅₀ 25 nM) for the α3β4 nAChR subtype. …”
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Artificial Fluorescent Glucosinolates (F-GSLs) Are Transported by the Glucosinolate Transporters GTR1/2/3 by Christa Kanstrup, Claire C. Jimidar, Josip Tomas, Giuliano Cutolo, Christoph Crocoll, Marie Schuler, Philipp Klahn, Arnaud Tatibouët, Hussam Hassan Nour-Eldin

“…We generated seven artificial glucosinolates (either aliphatic or benzenic) bearing different fluorophores (Fluorescein, BODIPY, Rhodamine, Dansylamide, and NBD) and investigated the ability of GTR1/2/3 from <i>Arabidopsis thaliana</i> to import the fluorescent glucosinolates (F-GSLs) into oocytes from <i>Xenopus laevis</i>. Five out of the seven F-GSLs synthesized were imported by at least one of the GTRs. …”
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