Inhibition of the Aquaporin-1 Cation Conductance by Selected Furan Compounds Reduces Red Blood Cell Sickling by Pak Hin Chow, Charles D. Cox, Charles D. Cox, Jinxin V. Pei, Nancy Anabaraonye, Saeed Nourmohammadi, Sam W. Henderson, Boris Martinac, Boris Martinac, Osheiza Abdulmalik, Andrea J. Yool

“…Human AQP1 channels expressed in Xenopus oocytes were evaluated for sensitivity to 5-HMF and four derivatives known to have differential efficacies in preventing RBC sickling. …”
Get full text

Comparative proteomic analysis of tail regeneration in the green anole lizard, Anolis carolinensis by Cindy Xu, Elizabeth D. Hutchins, Walter Eckalbar, Ken Pendarvis, Derek M. Benson, Douglas F. Lake, Fiona M. McCarthy, Kenro Kusumi

“…KEY POINTS This research is highly interdisciplinary, combining our previous analyses with these most recent findings: Appendage regeneration is a conserved trait among vertebrates and has been characterized in animals ranging from teleost fish (zebrafish), urodele amphibians (axolotl), anuran amphibians (Xenopus frog), squamate reptiles (various species of lizards), and even crocodilians (American alligator). …”
Get full text

TPX2 enhances the transcription factor activation of PXR and enhances the resistance of hepatocellular carcinoma cells to antitumor drugs by Hongbo Wang, Fang Chu, Xiao-feng Zhang, Peng Zhang, Li-xin Li, Yun-long Zhuang, Xiao-feng Niu, Xi He, Zhi-jie Li, Ying Bai, Da Mao, Zhen-wen Liu, Da-li Zhang, Bo-an Li

“…The target protein for the Xenopus plus end-directed kinesin-like protein (Xklp2) known as TPX2 that was previously considered as a tubulin regulator, also functions as the regulator of some transcription factors and pro-oncogenes in human malignances. …”
Get full text

MicroRNAs Regulate TASK‐1 and Are Linked to Myocardial Dilatation in Atrial Fibrillation by Felix Wiedmann, Manuel Kraft, Stefan Kallenberger, Antonius Büscher, Amelie Paasche, Pablo L. Blochberger, Timon Seeger, Natasa Jávorszky, Gregor Warnecke, Rawa Arif, Jamila Kremer, Matthias Karck, Norbert Frey, Constanze Schmidt

“…Thereby, we observed that miR‐34a increases TASK‐1 expression and current and further decreases the resting membrane potential of Xenopus laevis oocytes, heterologously expressing hTASK‐1. …”
Get full text

Identification and Functional Analysis of the First Aquaporin from Striped Stem Borer, Chilo suppressalis by Ming-Xing Lu, Ming-Xing Lu, Dan-Dan Pan, Dan-Dan Pan, Jing Xu, Jing Xu, Yang Liu, Gui-Rong Wang, Yu-Zhou Du, Yu-Zhou Du

“…Our data revealed that the Xenopus oocytes expressing CsDrip1 indicated CsDrip1 could transport water instead of glycerol, trehalose and urea. …”
Get full text

Pharmacological rescue of specific long QT variants of KCNQ1/KCNE1 channels by Xinle Zou, Xiaoan Wu, Kevin J. Sampson, Henry M. Colecraft, H. Peter Larsson, Robert S. Kass

“…In this paper, we used expression of IKS channels in Chinese hamster ovary (CHO) cells and Xenopus oocytes to study the potential of these two drugs (ML277 and R-L3) for the rescue of LQT1 and LQT5 mutant channels. …”
Get full text

Proteoliposomes reconstituted with human aquaporin-1 reveal novel single-ion-channel properties by Sam W. Henderson, Yoshitaka Nakayama, Murray L. Whitelaw, John B. Bruning, Peter A. Anderson, Stephen D. Tyerman, Sunita A. Ramesh, Boris Martinac, Andrea J. Yool

“…This study probed properties of hAQP1 ion channels using proteoliposomes, which, unlike conventional cell-based systems such as Xenopus laevis oocytes, are relatively free of background ion channels. …”
Get full text

Recombinant Production, NMR Solution Structure, and Membrane Interaction of the Phα1β Toxin, a TRPA1 Modulator from the Brazilian Armed Spider <i>Phoneutria nigriventer</i> by Ekaterina N. Lyukmanova, Pavel A. Mironov, Dmitrii S. Kulbatskii, Mikhail A. Shulepko, Alexander S. Paramonov, Elizaveta M. Chernaya, Yulia A. Logashina, Yaroslav A. Andreev, Mikhail P. Kirpichnikov, Zakhar O. Shenkarev

“…Surprisingly, 10 µM Phα1β significantly increases the amplitude of diclofenac-evoked currents and does not affect the allyl isothiocyanate (AITC)-evoked currents through the rat TRPA1 channel expressed in <i>Xenopus</i> oocytes. Targeting several unrelated ion channels, membrane binding, and the modulation of TRPA1 channel activity allow for considering Phα1β as a gating modifier toxin, probably interacting with S1–S4 gating domains from a membrane-bound state.…”
Get full text

Anxiolytic, Analgesic and Anti-Inflammatory Effects of Peptides Hmg 1b-2 and Hmg 1b-4 from the Sea Anemone <i>Heteractis magnifica</i> by Irina N. Gladkikh, Anna A. Klimovich, Rimma S. Kalina, Yulia V. Kozhevnikova, Timur A. Khasanov, Dmitry I. Osmakov, Sergey G. Koshelev, Margarita M. Monastyrnaya, Yaroslav A. Andreev, Elena V. Leychenko, Sergey A. Kozlov

“…Two sea anemone toxins, native Hmg 1b-2 and recombinant Hmg 1b-4, both related to APETx-like peptides, inhibited the transient current component of human ASIC3-Δ20 expressed in <i>Xenopus laevis</i> oocytes, but only Hmg 1b-2 inhibited the rat ASIC3 transient current. …”
Get full text

Direct identification of A-to-I editing sites with nanopore native RNA sequencing by Nguyen, Tram Anh, Heng, Joel Jia Wei, Kaewsapsak, Pornchai, Kok, Louis Eng Piew, Stanojević, Dominik, Liu, Hao, Cardilla, Angelysia, Praditya, Albert, Yi, Zirong, Lin, Mingwan, Aw, Ashley Jong Ghut, Ho, Yin Ying, Peh, Esther Kai Lay, Wang, Yuanming, Zhong, Qixing, Heraud-Farlow, Jacki, Xue, Shifeng, Reversade, Bruno, Walkley, Carl, Ho, Ying Swan, Šikić, Mile, Wan, Yue, Tan, Meng How

“…Furthermore, we demonstrated their utility on the transcriptomes of human, mouse and Xenopus. Our approach expands the toolkit for studying adenosine-to-inosine editing and can be further extended to investigate other RNA modifications.…”
Get full text

Investigating break induced replication and template switching in fission yeast by Laksir, Y

“…However, recent work, using the Xenopus egg extract system to study replication fork-SSB collisions in-vitro, has shown that both leading and lagging template strand SSBs cause seDSBs. …”

Targeting GABA_C Receptors Improves Post-Stroke Motor Recovery by Petra S. van Nieuwenhuijzen, Kim Parker, Vivian Liao, Josh Houlton, Hye-Lim Kim, Graham A. R. Johnston, Jane R. Hanrahan, Mary Chebib, Andrew N. Clarkson

“…(<i>R</i>)-4-amino-cyclopent-1-enyl butylphosphinic acid (<i>R</i>)-4-ACPBPA and (<i>S</i>)-4-ACPBPA were assessed using 2-elecotrode voltage electrophysiology in <i>Xenopus laevis</i> oocytes. Stroke mice were treated for 4 weeks with either vehicle, the α5-selective negative allosteric modulator, L655,708, or the <i>ρ</i>1/2 antagonists, (<i>R</i>)-4-ACPBPA and (<i>S</i>)-4-ACPBPA respectively from 3 days post-stroke. …”
Get full text

<span style="font-variant: small-caps">d</span>-Amino Acid Substitution of α-Conotoxin RgIA Identifies its Critical Residues and Improves the Enzymatic Stability by Jie Ren, Xiaopeng Zhu, Pan Xu, Rui Li, Ying Fu, Shuai Dong, Dongting Zhangsun, Yong Wu, Sulan Luo

“…The activity of each variant was investigated against rat and human &#945;9&#945;10 nAChRs, which were expressed in <i>Xenopus oocytes</i>. Experimental assays showed that 14 out of 15 analogues had a substantial reduction in potency towards rat &#945;9&#945;10 nAChR. …”
Get full text

The intertwining of Zn-finger motifs and abiotic stress tolerance in plants: Current status and future prospects by Debojyoti Moulick, Karma Landup Bhutia, Sukamal Sarkar, Anirban Roy, Udit Nandan Mishra, Biswajit Pramanick, Biswajit Pramanick, Sagar Maitra, Tanmoy Shankar, Swati Hazra, Milan Skalicky, Marian Brestic, Marian Brestic, Viliam Barek, Akbar Hossain

“…The Zn-finger was first reported as a repeated Zn-binding motif, comprising conserved cysteine (Cys) and histidine (His) ligands, in Xenopus laevis oocytes as a transcription factor (TF) IIIA (or TFIIIA). …”
Get full text

Vacuolar nitrate efflux requires multiple functional redundant nitrate transporter in Arabidopsis thaliana by Yu-Ting Lu, Yu-Ting Lu, De-Fen Liu, De-Fen Liu, Ting-Ting Wen, Ting-Ting Wen, Zi-Jun Fang, Si-Ying Chen, Si-Ying Chen, Hui Li, Ji-Ming Gong, Ji-Ming Gong

“…Ectopic expression in Xenopus laevis oocytes revealed that they mediate low-affinity nitrate transport. …”
Get full text

Interaction of Purinergic P2X4 and P2X7 Receptor Subunits by Markus Schneider, Kirsten Prudic, Anja Pippel, Manuela Klapperstück, Ursula Braam, Christa E. Müller, Günther Schmalzing, Fritz Markwardt

“…To resolve this issue, we coexpressed P2X4 and P2X7 receptor subunits labeled with green (EGFP) and red (TagRFP) fluorescent proteins in Xenopus laevis oocytes and investigated a putative physical interaction between the fusion proteins by Förster resonance energy transfer (FRET). …”
Get full text

From Synthetic Fragments of Endogenous Three-Finger Proteins to Potential Drugs by Elena V. Kryukova, Natalia S. Egorova, Denis S. Kudryavtsev, Dmitry S. Lebedev, Ekaterina N. Spirova, Maxim N. Zhmak, Aleksandra I. Garifulina, Igor E. Kasheverov, Igor E. Kasheverov, Yuri N. Utkin, Victor I. Tsetlin, Victor I. Tsetlin

“…In competition for the ligand-binding domain of the α9 nAChR subunit, SSS and Lynx1 fragments had IC50 values of about 40 µM, which correlates with the value found for the latter with the rat α9α10 nAChR expressed in the Xenopus oocytes. Thus, the activity of these synthetic peptides against muscle-type and α9α10 nAChRs indicates that they may be useful in design of novel myorelaxants and analgesics.…”
Get full text

Tetrapeptide Ac-HAEE-NH₂ Protects α4β2 nAChR from Inhibition by Aβ by Evgeny P. Barykin, Aleksandra I. Garifulina, Anna P. Tolstova, Anastasia A. Anashkina, Alexei A. Adzhubei, Yuri V. Mezentsev, Irina V. Shelukhina, Sergey A. Kozin, Victor I. Tsetlin, Alexander A. Makarov

“…Finally, using two-electrode voltage clamp in <i>Xenopus laevis</i> oocytes, we showed that Ac-HAEE-NH₂ tetrapeptide completely abolishes the Aβ₄₂-induced inhibition of α4β2 nAChR. …”
Get full text

Sigma-1 Receptor Plays a Negative Modulation on N-type Calcium Channel by Kang Zhang, Zhe Zhao, Liting Lan, Xiaoli Wei, Liyun Wang, Xiaoyan Liu, Haitao Yan, Jianquan Zheng

“…Co-expression of the sigma-1 receptors and the N-type calcium channels in Xenopus oocytes presented a decrease of N-type Ca2+ current amplitude with an increase of sigma-1 receptor expression. …”
Get full text

The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling by Victoria H. Ng, Zachary Spencer, Leif R. Neitzel, Anmada Nayak, Matthew A. Loberg, Chen Shen, Sara N. Kassel, Heather K. Kroh, Zhenyi An, Christin C. Anthony, Jamal M. Bryant, Amanda Lawson, Lily Goldsmith, Hassina Benchabane, Amanda G. Hansen, Jingjing Li, Starina D’Souza, Andres M. Lebensohn, Rajat Rohatgi, William A. Weiss, Vivian L. Weiss, Charles Williams, Charles C. Hong, David J. Robbins, Yashi Ahmed, Ethan Lee

“…We find that the USP46 complex is similarly required for Wnt signaling in Xenopus and zebrafish embryos. We demonstrate that Wnt signaling promotes the association between the USP46 complex and cell surface Wnt coreceptor, LRP6. …”
Get full text