Role of matrix metalloproteinase-9 in neurodevelopmental deficits and experience-dependent plasticity in Xenopus laevis by Sayali V Gore, Eric J James, Lin-chien Huang, Jenn J Park, Andrea Berghella, Adrian C Thompson, Hollis T Cline, Carlos D Aizenman

“…In Xenopus tadpoles, VPA exposure results in excess local synaptic connectivity, disrupted social behavior and increased seizure susceptibility. …”
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Cell-free reconstitution of peroxisomal matrix protein import using Xenopus egg extract by Michael L. Skowyra, Tom A. Rapoport

“…Here, we present a protocol for reconstituting matrix protein import using Xenopus egg extract. We describe how extract is prepared, how to replace endogenous PEX5 with recombinant versions, and how to perform and interpret a peroxisomal import reaction using a fluorescent cargo.For complete details on the use and execution of this protocol, please refer to Skowyra and Rapoport (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Functional Analysis of Gastric Tight Junction Proteins in <i>Xenopus laevis</i> Oocytes by Laura Stein, Nora Brunner, Salah Amasheh

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Adverse Effect of Metallic Gold and Silver Nanoparticles on <i>Xenopus laevis</i> Embryogenesis by Rosa Carotenuto, Margherita Tussellino, Sabato Fusco, Giovanna Benvenuto, Fabio Formiggini, Bice Avallone, Chiara Maria Motta, Chiara Fogliano, Paolo Antonio Netti

“…In this study, we tested the effects of commercial AuNPs and AgNPs, widely used in many fields for their features, on the early development of <i>Xenopus laevis</i>, an anuran amphibian key model species in toxicity testing. …”
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Publisher Correction: Cell landscape of larval and adult Xenopus laevis at single-cell resolution by Yuan Liao, Lifeng Ma, Qile Guo, Weigao E, Xing Fang, Lei Yang, Fanwei Ruan, Jingjing Wang, Peijing Zhang, Zhongyi Sun, Haide Chen, Zhongliang Lin, Xueyi Wang, Xinru Wang, Huiyu Sun, Xiunan Fang, Yincong Zhou, Ming Chen, Wanhua Shen, Guoji Guo, Xiaoping Han

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Liver development during Xenopus tropicalis metamorphosis is controlled by T3-activation of WNT signaling by Yuta Tanizaki, Shouhong Wang, Hongen Zhang, Yuki Shibata, Yun-Bo Shi

“…Liver remodeling during anuran metamorphosis resembles liver maturation in mammals and is controlled by T3. We generated Xenopus tropicalis animals with both TRα and TRβ genes knocked out and found that TR double knockout liver had developmental defects such as reduced cell proliferation and failure to undergo hepatocyte hypertrophy or activate urea cycle gene expression. …”
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Mitotic spindle scaling during Xenopus development by kif2a and importin α by Jeremy D Wilbur, Rebecca Heald

“…Thus, spindle size in Xenopus development is coupled to cell size through a ratiometric mechanism controlling microtubule destabilization.…”
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Revealing mitf functions and visualizing allografted tumor metastasis in colorless and immunodeficient Xenopus tropicalis by Rensen Ran, Lanxin Li, Tingting Xu, Jixuan Huang, Huanhuan He, Yonglong Chen

“…Here, we employed a mitf −/−/prkdc −/−/il2rg −/− triple-knockout strategy to establish a colorless and immunodeficient amphibian model of Xenopus tropicalis. By disrupting the mitf gene, we observed the loss of melanophores, xanthophores, and granular glands in Xenopus tropicalis. …”
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Multiple mechanisms promote the retained expression of gene duplicates in the tetraploid frog Xenopus laevis.

“…We tested the applicability of three types of mechanisms for promoting the retained expression of duplicated genes in 290 expressed paralogs of the tetraploid clawed frog, Xenopus laevis. Tests were based on explicit expectations concerning the ka/ks ratio, and the number and location of nonsynonymous substitutions after duplication. …”
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Regulation of rhodopsin-eGFP distribution in transgenic xenopus rod outer segments by light. by Mohammad Haeri, Peter D Calvert, Eduardo Solessio, Edward N Pugh, Barry E Knox

“…The rod outer segment (OS), comprised of tightly stacked disk membranes packed with rhodopsin, is in a dynamic equilibrium governed by a diurnal rhythm with newly synthesized membrane inserted at the OS base balancing membrane loss from the distal tip via disk shedding. Using transgenic Xenopus and live cell confocal imaging, we found OS axial variation of fluorescence intensity in cells expressing a fluorescently tagged rhodopsin transgene. …”
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Multiple mechanisms promote the retained expression of gene duplicates in the tetraploid frog Xenopus laevis. by Frédéric J J Chain, Ben J Evans

“…We tested the applicability of three types of mechanisms for promoting the retained expression of duplicated genes in 290 expressed paralogs of the tetraploid clawed frog, Xenopus laevis. Tests were based on explicit expectations concerning the ka/ks ratio, and the number and location of nonsynonymous substitutions after duplication. …”
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TMEM79/MATTRIN defines a pathway for Frizzled regulation and is required for Xenopus embryogenesis by Maorong Chen, Nathalia Amado, Jieqiong Tan, Alice Reis, Mengxu Ge, Jose Garcia Abreu, Xi He

“…Tmem79 and Usp8 genes have a pre-bilaterian origin, and Tmem79 inhibition of Usp8 and Wnt signaling is required for anterior neural development and gastrulation in Xenopus embryos. TMEM79 is a predisposition gene for Atopic dermatitis, suggesting deregulation of Wnt/FZD signaling a possible cause for this most common yet enigmatic inflammatory skin disease.…”
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Identification of tumor-related genes via RNA sequencing of tumor tissues in Xenopus tropicalis by Kazuki Kitamura, Takayoshi Yamamoto, Haruki Ochi, Makoto Suzuki, Nanoka Suzuki, Takeshi Igawa, Tadashi Yoshida, Mitsuru Futakuchi, Hajime Ogino, Tatsuo Michiue

“…Here, we analyzed gene expression in tumor tissues of three individuals in Xenopus tropicalis and identified 55 differentially expressed genes (DEGs). …”
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Developmental eye motion plasticity after unilateral embryonic ear removal in Xenopus laevis by Clayton Gordy, Hans Straka

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Xbra modulates the activity of linker region phosphorylated Smad1 during Xenopus development by Santosh Kumar, Zobia Umair, Vijay Kumar, Ravi Shankar Goutam, Soochul Park, Unjoo Lee, Jaebong Kim

“…Taken together, the results suggests Xbra-dependent cooperative interplays between Fgf/Erk and Bmp/Smad1 signaling during lateral mesoderm specification in Xenopus embryos.…”
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Deep transcriptome profiling reveals limited conservation of A-to-I RNA editing in Xenopus by Tram Anh Nguyen, Jia Wei Joel Heng, Yan Ting Ng, Rui Sun, Shira Fisher, Gokce Oguz, Pornchai Kaewsapsak, Shifeng Xue, Bruno Reversade, Adaikalavan Ramasamy, Eli Eisenberg, Meng How Tan

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Regional expression of Pax7 in the brain of Xenopus laevis during embryonic and larval development by Sandra eBandín, Ruth eMorona, Nerea eMoreno, Agustín eGonzález

“…Using sensitive immunohistochemical techniques we have analyzed the spatiotemporal pattern of Pax7 expression in the brain of the anuran amphibian Xenopus laevis, during development. Pax7 expression was first detected in early embryos in the basal plate of prosomere 3, roof and alar plates of prosomere 1 and mesencephalon, and the alar plate of rhombomere 1. …”
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Novel predator-induced phenotypic plasticity by hemoglobin and physiological changes in the brain of Xenopus tropicalis by Tsukasa Mori, Kazumasa Machida, Yuki Kudou, Masaya Kimishima, Kaito Sassa, Naoko Goto-Inoue, Ryuhei Minei, Atsushi Ogura, Yui Kobayashi, Kentaro Kamiya, Daiki Nakaya, Naoyuki Yamamoto, Akihiko Kashiwagi, Keiko Kashiwagi

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A non-transcriptional function of Yap regulates the DNA replication program in Xenopus laevis by Rodrigo Meléndez García, Olivier Haccard, Albert Chesneau, Hemalatha Narassimprakash, Jérôme Roger, Muriel Perron, Kathrin Marheineke, Odile Bronchain

“…In multicellular eukaryotic organisms, the initiation of DNA replication occurs asynchronously throughout S-phase according to a regulated replication timing program. Here, using Xenopus egg extracts, we showed that Yap (Yes-associated protein 1), a downstream effector of the Hippo signalling pathway, is required for the control of DNA replication dynamics. …”
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Visuospatial information in the retinotectal system of xenopus before correct image formation by the developing eye. by Richards, B, van Rheede, J, Akerman, C

“…The retinotectal pathway of Xenopus laevis is a well-established experimental model for studying activity-dependent processes during visual system development. …”