SOMA: a single oligonucleotide mutagenesis and cloning approach. by Thorsten Pfirrmann, Ashwin Lokapally, Claes Andréasson, Per Ljungdahl, Thomas Hollemann

“…We successfully use such a reporter to assess the in vivo knock-down quality of morpholinos in Xenopus laevis embryos. In a second example, we show how to use a SOMA-based protocol for restriction-site independent cloning to generate chimeric proteins by domain swapping between the two human hRMD5a and hRMD5b isoforms. …”
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Multienzyme activity profiling for evaluation of cell‐to‐cell variability of metabolic state by Govind S. Gill, Michael C. Schultz

“…We study individual cells of types that can be identified during the post‐mitotic phase of oogenesis in Xenopus laevis. Whole‐cell homogenates of isolated oocytes are used for kinetic analysis of enzymes, with a focus on the initial reaction rate. …”
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Microglial trogocytosis and the complement system regulate axonal pruning in vivo by Tony KY Lim, Edward S Ruthazer

“…Here, we directly observe axon trogocytosis by microglia in vivo in the developing Xenopus laevis retinotectal circuit. We show that microglia regulate pruning of retinal ganglion cell axons and are important for proper behavioral response to dark and bright looming stimuli. …”
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Bacterial lipopolysaccharides can initiate regeneration of the Xenopus tadpole tail by Thomas F. Bishop, Caroline W. Beck

“…Summary: Tadpoles of the frog Xenopus laevis can regenerate tails except for a short “refractory” period in which they heal rather than regenerate. …”
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Xenopus Pkdcc1 and Pkdcc2 Are Two New Tyrosine Kinases Involved in the Regulation of JNK Dependent Wnt/PCP Signaling Pathway. by Marta Vitorino, Ana Cristina Silva, José Manuel Inácio, José Silva Ramalho, Michal Gur, Abraham Fainsod, Herbert Steinbeisser, José António Belo

“…Here, we identified two new members of the PKDCC family, Pkdcc1 and Pkdcc2 from Xenopus laevis. Interestingly, our knockdown experiments revealed that these two proteins are both involved on blastopore and neural tube closure during gastrula and neurula stages, respectively. …”
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Cip29 is phosphorylated following activation of the DNA damage response in Xenopus egg extracts. by Janet Holden, Elaine M Taylor, Howard D Lindsay

“…In this study, we have used a Xenopus laevis egg extract system to analyse the DNA damage-dependent phosphorylation of a putative RNA export factor, Cip29. …”
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An NF-kappaB and slug regulatory loop active in early vertebrate mesoderm. by Chi Zhang, Timothy F Carl, Evan D Trudeau, Thomas Simmet, Michael W Klymkowsky

“…BACKGROUND:In both Drosophila and the mouse, the zinc finger transcription factor Snail is required for mesoderm formation; its vertebrate paralog Slug (Snai2) appears to be required for neural crest formation in the chick and the clawed frog Xenopus laevis. Both Slug and Snail act to induce epithelial to mesenchymal transition (EMT) and to suppress apoptosis. …”
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Intrinsically disordered regions are not sufficient to direct the compartmental localization of nucleolar proteins in the nucleus. by Emily D Lavering, Maunika Gandhamaneni, Daniel L Weeks

“…We investigated whether the intrinsically disordered regions from several proteins provided the information needed to establish specific compartment localization using Xenopus laevis oocytes. For the proteins we tested, the disordered regions were not sufficient to direct specific domain localization and appear dispensable with respect to compartmentalization. …”
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BDNF signaling in correlation-dependent structural plasticity in the developing visual system. by Elena Kutsarova, Anne Schohl, Martin Munz, Alex Wang, Yuan Yuan Zhang, Olesia M Bilash, Edward S Ruthazer

“…We used visual stimulation to control the correlation structure of neural activity in a few ipsilaterally projecting (ipsi) retinal ganglion cell (RGC) axons with respect to the majority contralateral eye inputs in the optic tectum of albino Xenopus laevis tadpoles. Multiphoton live imaging of ipsi axons, combined with specific targeted disruptions of brain-derived neurotrophic factor (BDNF) signaling, revealed that both presynaptic p75NTR and TrkB are required for Stentian axonal branch addition, whereas presumptive postsynaptic BDNF signaling is necessary for Hebbian axon stabilization. …”
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CRIM1 complexes with ß-catenin and cadherins, stabilizes cell-cell junctions and is critical for neural morphogenesis. by Virgilio G Ponferrada, Jieqing Fan, Jefferson E Vallance, Shengyong Hu, Aygun Mamedova, Scott A Rankin, Matthew Kofron, Aaron M Zorn, Rashmi S Hegde, Richard A Lang

“…Cysteine-rich motor neuron protein 1 (CRIM1) is a single-pass (type 1) transmembrane protein that is expressed in neural structures beginning at the neural plate stage. In the frog Xenopus laevis, loss of function studies using CRIM1 antisense morpholino oligonucleotides resulted in a failure of neural development. …”
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High-sensitivity real-time imaging of dual protein-protein interactions in living subjects using multicolor luciferases. by Naoki Hida, Muhammad Awais, Masaki Takeuchi, Naoto Ueno, Mayuri Tashiro, Chiyo Takagi, Tanuja Singh, Makoto Hayashi, Yoshihiro Ohmiya, Takeaki Ozawa

“…We demonstrate its utility in spatiotemporal characterization of Smad1-Smad4 and Smad2-Smad4 interactions in early developing stages of a single living Xenopus laevis embryo. We also describe the value of this method by application of specific protein-protein interactions in cell cultures and living mice. …”
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Electrophysiological Studies Revealed CaM1-Mediated Regulation of the Arabidopsis Calcium Channel CNGC12 by Zhengli Zhang, Congcong Hou, Wang Tian, Legong Li, Huifen Zhu

“…Here, we determined the channel activities of CNGC11 and CNGC12 utilizing the two-electrode voltage-clamp technique in the Xenopus laevis oocyte heterologous expression system. …”
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Various nuclear reprogramming systems using egg and oocyte materials by Kei MIYAMOTO

“…Other experimental systems are also available to study maternal reprogramming, such as nuclear transfer to Xenopus laevis oocytes at the germinal vesicle stage, treatment with extracts obtained from eggs or oocytes, and induced pluripotency with overexpressed maternal factors. …”
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RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA), an Assay for Detecting RNA-Protein Complexes in Intact Cells. by Ioannis M Roussis, Matthew Guille, Fiona A Myers, Garry P Scarlett

“…As proof of principle we used the well-characterised interaction of the Xenopus laevis Staufen RNA binding protein with Vg1 mRNA, the complex of which co-localises to the vegetal pole of Xenopus oocytes. …”
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Surprisingly simple mechanical behavior of a complex embryonic tissue. by Michelangelo von Dassow, James A Strother, Lance A Davidson

“…Hence we expect highly non-linear and loading-rate dependent tissue mechanical properties in embryos.We used micro-aspiration to test whether a simple linear viscoelastic model was sufficient to describe the mechanical behavior of gastrula stage Xenopus laevis embryonic tissue in vivo. We tested whether these embryonic tissues change their mechanical properties in response to mechanical stimuli but found no evidence of changes in the viscoelastic properties of the tissue in response to stress or stress application rate. …”
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SGK3 Sensitivity of Large-Conductance Ca2+-Activated K+ Channel by Musaab Ahmed, Myriam Fezai, Florian Lang

“…Methods: cRNA encoding the Ca2+ insensitive BK channel mutant BKM513I+Δ899-903 was injected into Xenopus laevis oocytes without or with additional injection of cRNA encoding wild-type SGK3, constitutively active S419DSGK3, or catalytically inactive K191NSGK3. …”
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Phospholipase C and diacylglycerol mediate olfactory responses to amino acids in the main olfactory epithelium of an amphibian. by Alfredo Sansone, Thomas Hassenklöver, Adnan S Syed, Sigrun I Korsching, Ivan Manzini

“…We have recently shown that amino acid responses in the main olfactory epithelium (MOE) of larval Xenopus laevis segregate into a lateral and a medial processing stream, and that the former is part of a vomeronasal type 2 receptor expression zone in the MOE. …”
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Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging. by Yong Qian, Danielle M Orozco Cosio, Kiryl D Piatkevich, Sarah Aufmkolk, Wan-Chi Su, Orhan T Celiker, Anne Schohl, Mitchell H Murdock, Abhi Aggarwal, Yu-Fen Chang, Paul W Wiseman, Edward S Ruthazer, Edward S Boyden, Robert E Campbell

“…We characterized the performance of the new NIR GECIs in cultured cells, acute mouse brain slices, and Caenorhabditis elegans and Xenopus laevis in vivo. Our results demonstrate that NIR-GECO2 and NIR-GECO2G provide substantial improvements over NIR-GECO1 for imaging of neuronal Ca2+ dynamics.…”
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Dimerization and heme binding are conserved in amphibian and starfish homologues of the microRNA processing protein DGCR8. by Rachel Senturia, Arthur Laganowsky, Ian Barr, Ian Barr, Brooke D Scheidemantle, Feng Guo

“…Additionally, we present the crystal structure of the Xenopus laevis dimerization domain. The structure is very similar to that of human DGCR8. …”
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β-adrenergic receptor regulates embryonic epithelial extensibility through actomyosin inhibition by Yohei Mizoguchi, Kaoru Nakashima, Ayato Sato, Asako Shindo

“…Chemical screens on Xenopus laevis embryos pinpointed ADRB2 as a principal regulator. …”
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