Persistent fibrosis, hypertrophy and sarcomere disorganisation after endoscopy-guided heart resection in adult Xenopus. by Lindsey Marshall, Céline Vivien, Fabrice Girardot, Louise Péricard, Barbara A Demeneix, Laurent Coen, Norin Chai

“…We thus developed an endoscopy-based resection method to explore the consequences of cardiac injury in adult Xenopus laevis. This method allowed in situ live heart observation, standardised tissue amputation size and reproducibility. …”
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Ataxia-linked SLC1A3 mutations alter EAAT1 chloride channel activity and glial regulation of CNS function by Qianyi Wu, Azman Akhter, Shashank Pant, Eunjoo Cho, Jin Xin Zhu, Alastair Garner, Tomoko Ohyama, Emad Tajkhorshid, Donald J. van Meyel, Renae M. Ryan

“…Here we investigated the effects of 5 additional EA6-related mutations on hEAAT1 function in Xenopus laevis oocytes, and on CNS function in a Drosophila melanogaster model of locomotor behavior. …”
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Depolarization of the conductance-voltage relationship in the NaV1.5 mutant, E1784K, is due to altered fast inactivation. by Colin H Peters, Alec Yu, Wandi Zhu, Jonathan R Silva, Peter C Ruben

“…We measured ionic currents and gating currents at pH 7.4 and pH 6.0 in Xenopus laevis oocytes. Contrary to our expectation, the movement of gating charges is shifted to more hyperpolarized membrane potentials by E1784K. …”
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Antennal-expressed ammonium transporters in the malaria vector mosquito Anopheles gambiae. by R Jason Pitts, Stephen L Derryberry, Fadi E Pulous, Laurence J Zwiebel

“…Functional expression of AgAmt in Xenopus laevis oocytes facilitates inward currents in response to both ammonium and methylammonium, while AgRh50 is able to partially complement a yeast ammonium transporter mutant strain, validating their conserved roles as ammonium transporters. …”
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A Developmental Requirement for HIRA-Dependent H3.3 Deposition Revealed at Gastrulation in Xenopus by Emmanuelle Szenker, Nicolas Lacoste, Geneviève Almouzni

“…To assess the importance of the H3.3 histone variant and its dedicated histone chaperone HIRA, we used an established developmental model, Xenopus laevis. After the early rapid divisions exploiting a large maternal pool of both replicative H3.2 and replacement H3.3, H3.3 transcripts show a distinct peak of expression at gastrulation. …”
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Development of a new approach for targeted gene editing in primordial germ cells using TALENs in Xenopus by Keisuke Nakajima, Yoshio Yaoita

“…We used the 3′ untranslated region of DEADSouth gene (DS-3′) of Xenopus tropicalis to solve this problem, because the addition of the DS-3′ to mRNA is known to induce primordial germ cell (PGC)-specific expression and reduce the stability in somatic cells of mRNA in Xenopus laevis. At first, we inserted the X. tropicalis DS-3′ downstream of the EGFP termination codon and confirmed that the EGFP expression was specifically detected in PGCs for three weeks. …”
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Mode of Action of Neonicotinoid Insecticides Imidacloprid and Thiacloprid to the Cockroach Pameα7 Nicotinic Acetylcholine Receptor by Alison Cartereau, Emiliane Taillebois, Jean-Yves Le Questel, Steeve H. Thany

“…The functional expression of the cockroach Pameα7 nicotinic acetylcholine receptor subunit has been previously studied, and was found to be able to form a homomeric receptor when expressed in <i>Xenopus laevis</i> oocytes. In this study, we found that the neonicotinoid insecticide imidacloprid is unable to activate the cockroach Pameα7 receptor, although thiacloprid induces low inward currents, suggesting that it is a partial agonist. …”
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acr-23 Encodes a monepantel-sensitive channel in Caenorhabditis elegans. by Lucien Rufener, Nicola Bedoni, Roland Baur, Samantha Rey, Dominique A Glauser, Jacques Bouvier, Robin Beech, Erwin Sigel, Alessandro Puoti

“…Finally, we present reconstitution of the C. elegans ACR-23 receptor in Xenopus laevis oocytes and provide direct evidence of its modulation by monepantel. …”
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Two octaves spanning photoacoustic microscopy by Gianni Nteroli, Manoj K. Dasa, Giulia Messa, Stella Koutsikou, Magalie Bondu, Peter M. Moselund, Christos Markos, Ole Bang, Adrian Podoleanu, Adrian Bradu

“…Abstract In this study, for the first time, a Photoacoustic Microscopy instrument driven by a single optical source operating over a wide spectral range (475–2400 nm), covering slightly more than two octaves is demonstrated. Xenopus laevis tadpoles were imaged in vivo using the whole spectral range of 2000 nm of a supercontinuum optical source, and a novel technique of mapping absorbers is also demonstrated, based on the supposition that only one chromophore contributes to the photoacoustic signal of each individual voxel in the 3D photoacoustic image. …”
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An isoprenylation and palmitoylation motif promotes intraluminal vesicle delivery of proteins in cells from distant species. by Clara L Oeste, Mario Pinar, Kay O Schink, Javier Martínez-Turrión, Harald Stenmark, Miguel A Peñalva, Dolores Pérez-Sala

“…In the lower species, neither the C-terminal sequence of RhoB, nor the endosomal distribution of its homologs are conserved; in spite of this, CINCCKVL constructs also reach endolysosomes in Xenopus laevis and insect cells. Strikingly, this behavior is prominent in the filamentous ascomycete fungus Aspergillus nidulans, in which GFP-CINCCKVL is sorted into endosomes and vacuoles in a lipidation-dependent manner and allows monitoring endosomal movement in live fungi. …”
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Changes in Ca2+ Removal Can Mask the Effects of Geometry During IP3R Mediated Ca2+ Signals by Estefanía Piegari, Cecilia Villarruel, Silvina Ponce Dawson

“…In this paper we combine experiments performed in untreated and in progesterone-treated Xenopus laevis oocytes and mathematical models to investigate how the interplay between geometry (the IP3R spatial distribution) and dynamics (the processes that characterize the release, transport, and removal of cytosolic Ca2+) affects the resulting signals. …”
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RECQL4 is not critical for firing of human DNA replication origins by Laura Padayachy, Sotirios G. Ntallis, Thanos D. Halazonetis

“…Consistent with this sequence similarity, the Xenopus laevis homolog of RECQL4 has been implicated in initiating DNA replication in egg extracts. …”
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Structure of the arginine methyltransferase PRMT5-MEP50 reveals a mechanism for substrate specificity. by Meng-Chiao Ho, Carola Wilczek, Jeffrey B Bonanno, Li Xing, Janina Seznec, Tsutomu Matsui, Lester G Carter, Takashi Onikubo, P Rajesh Kumar, Man K Chan, Michael Brenowitz, R Holland Cheng, Ulf Reimer, Steven C Almo, David Shechter

“…To test a model in which MEP50 is critical for substrate recognition and orientation, we determined the crystal structure of Xenopus laevis PRMT5-MEP50 complexed with S-adenosylhomocysteine (SAH). …”
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Variability of Rheotaxis Behaviors in Larval Bullfrogs Highlights Species Diversity in Lateral Line Function. by Erika E A Brown, Andrea Megela Simmons

“…Previous research identified distinct differences in one such behavior, positive rheotaxis towards the source of a flow, in two tadpole species, the African clawed frog (Xenopus laevis; type 1) and the American bullfrog (Rana catesbeiana; type 4). …”
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Large, long range tensile forces drive convergence during Xenopus blastopore closure and body axis elongation by David R Shook, Eric M Kasprowicz, Lance A Davidson, Raymond Keller

“…Indirect evidence suggests that blastopore closure during gastrulation of anamniotes, including amphibians such as Xenopus laevis, depends on circumblastoporal convergence forces generated by the marginal zone (MZ), but direct evidence is lacking. …”
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A voltage dependent non-inactivating Na+ channel activated during apoptosis in Xenopus oocytes. by Ulrika H Englund, Jens Gertow, Katarina Kågedal, Fredrik Elinder

“…In the present investigation we examined whether ion channels are up-regulated in oocytes from the frog Xenopus laevis during apoptosis. The two-electrode voltage-clamp technique was used to record endogenous ion currents in the oocytes. …”
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Biomechanics and the thermotolerance of development. by Michelangelo von Dassow, Callie Johnson Miller, Lance A Davidson

“…To test this model we investigated how the mechanical behavior of embryonic tissue (Xenopus laevis) changed with temperature; we used a combination of micropipette aspiration to measure viscoelasticity, electrically induced contractions to measure cellular force generation, and confocal microscopy to measure endogenous contractility. …”
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Xenopus: An alternative model system for identifying muco-active agents. by Hyo Jung Sim, Sang-Hyun Kim, Kyung-Jae Myung, Taejoon Kwon, Hyun-Shik Lee, Tae Joo Park

“…In this study, we developed a high-throughput in vivo model system for identifying muco-active reagents using Xenopus laevis embryos. We tested mucus secretion under various conditions and developed a screening strategy to identify potential muco-regulators. …”
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Functional validation of novel levamisole resistance marker S168T in Haemonchus contortus by Alistair Antonopoulos, Claude L. Charvet, Kirsty Maitland, Stephen R. Doyle, Cédric Neveu, Roz Laing

“…Here, we used the Xenopus laevis oocyte expression system and two-electrode voltage-clamp electrophysiology to measure the functional impact of this S168T variant on the H. contortus levamisole-sensitive acetylcholine receptor, L-AChR-1.1. …”
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The development of the adult intestinal stem cells: Insights from studies on thyroid hormone-dependent amphibian metamorphosis by Shi Yun-Bo, Hasebe Takashi, Fu Liezhen, Fujimoto Kenta, Ishizuya-Oka Atsuko

“…We and others have studied the T3-dependent remodeling of the intestine in <it>Xenopus laevis</it>. Here we will highlight some of the recent findings on the origin of the adult intestinal stem cells. …”
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