Effect of N-linked glycosylation on hepatic lipase activity by G Stahnke, RC Davis, MH Doolittle, H Wong, MC Schotz, H Will

“…To directly test this hypothesis, Xenopus laevis oocytes were used to express native rat HL or HL lacking one or both N-linked glycosylation sites. …”
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Single-Particle Tracking Reveals Anti-Persistent Subdiffusion in Cell Extracts by Konstantin Speckner, Matthias Weiss

“…Extracts derived from the eggs of the clawfrog <i>Xenopus laevis</i>, for example, are known for their ability to support and mimic vital processes of cells, emphasizing the need to explore also the transport phenomena of nano-sized particles in such extracts. …”
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Functional and structural analysis of the internal ribosome entry site present in the mRNA of natural variants of the HIV-1. by Maricarmen Vallejos, Felipe Carvajal, Karla Pino, Camilo Navarrete, Marcela Ferres, Juan Pablo Huidobro-Toro, Bruno Sargueil, Marcelo López-Lastra

“…Cap-independent translational activity was demonstrated using bicistronic mRNAs in HeLa cells and in Xenopus laevis oocytes. The possibility that expression of the downstream cistron in these constructs was due to alternative splicing or to cryptic promoter activity was ruled out. …”
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Major histocompatibility complex based resistance to a common bacterial pathogen of amphibians. by Seth M Barribeau, Jandouwe Villinger, Bruce Waldman

“…To investigate the role of the major histocompatibility complex (MHC) in conferring pathogen resistance, we challenged Xenopus laevis tadpoles bearing different combinations of four MHC haplotypes (f, g, j, and r) with the bacterial pathogen Aeromonas hydrophila in two experiments. …”
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Expression of transposable elements in neural tissues during Xenopus development. by Fernando Faunes, Natalia Sanchez, Mauricio Moreno, Gonzalo H Olivares, Dasfne Lee-Liu, Leonardo Almonacid, Alex W Slater, Tomas Norambuena, Ryan J Taft, John S Mattick, Francisco Melo, Juan Larrain

“…Interestingly, although Tc1-2_Xt transcripts were not detected in Xenopus laevis embryos, transcripts from other two Tc1-like elements (TXr and TXz) presented a similar temporal and spatial pattern during X. laevis development. …”
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Prediction, design and characterisation of metal binding sites in antibodies by St. John Gregory, D

“…The final model was used as a basis for site-directed mutagenesis and mutant antibodies were produced using a Xenopus laevis oocyte expression system. Antigen binding studies showed the mutants to have an affinity for lysozyme equivalent to the parent antibody, while subsequent metal binding experiments have shown the new site to be capable of binding the transition metals cobalt, nickel, copper, zinc and cadmium.…”

Expression patterns of an Otx2 and an Otx5 orthologue in the urodele Pleurodeles waltl: implications on the evolutionary relationships between the balancers and cement gland in amp... by Sauka-Spengler, T, Germot, A, Shi, D, Mazan, S

“…These two genes, termed PwOtx2 and PwOtx5, share highly conserved expression domains with their gnathostome counterparts at tailbud stages, like the developing forebrain ( PwOtx2), or the embryonic eye and epiphysis ( PwOtx5). As in Xenopus laevis, both are also transcribed in the dorsal lip of the blastopore during gastrulation and in anterior parts of the neural plate during neurulation. …”

Comparative pharmacology and computational modelling yield insights into allosteric modulation of human alpha7 nicotinic acetylcholine receptors. by Sattelle, D, Buckingham, S, Akamatsu, M, Matsuda, K, Pienaar, I, Pienaar, I, Jones, A, Sattelle, B, Almond, A, Blundell, C

“…The human alpha7 nicotinic acetylcholine receptor (nAChR) subunit and its Caenorhabditis elegans homolog, ACR-16, can generate functional recombinant homomeric receptors when expressed in Xenopus laevis oocytes. Both nAChRs express robustly in the presence of the co-injected chaperone, RIC-3, and show striking differences in the actions of a type I positive allosteric modulator (PAM), ivermectin (IVM). …”

Evaluation of the developmental toxicity of 2-phenoxyethanol and clove oil anaesthetics using the Frog Embryo Teratogenesis Assay: Xenopus (FETAX) by D. Vrskova, H. Modra

“…The developmental toxicity of two anaesthetics, 2-phenoxyethanol and clove oil, used in aquaculture was evaluated using the Frog Embryo Teratogenesis Assay: Xenopus (FETAX) and the results were compared to outcomes in fish. Xenopus laevis embryos were exposed to 50, 100, 300, 500, 700 and 1000 mg/l of 2-phenoxyethanol or 1, 5, 10, 20, 30 and 40 mg/l of clove oil. …”
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Overexpression of the wheat aquaporin gene, TaAQP7, enhances drought tolerance in transgenic tobacco. by Shiyi Zhou, Wei Hu, Xiaomin Deng, Zhanbing Ma, Lihong Chen, Chao Huang, Chen Wang, Jie Wang, Yanzhen He, Guangxiao Yang, Guangyuan He

“…TaAQP7 exhibited high water channel activity in Xenopus laevis oocytes and TaAQP7 transcript was induced by dehydration, and treatments with polyethylene glycol (PEG), abscisic acid (ABA) and H(2)O(2). …”
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Gli studenti portano la scienza nello Spazio by Stefano Cartocci, Alessandro Fortuna, Cristina Meringolo, Monica Monici, Angela Maria Rizzo, Germana Galoforo

“…Gli studenti sono stati coinvolti nello studio della crescita e della rigenerazione dei girini di Xenopus laevis in assenza di gravità e hanno affrontato sia gli aspetti scientifico-biologici dell'esperimento sia quelli tecnologici relativi all’hardware necessario al mantenimento dei girini per 30 giorni nello Spazio e alle rilevazioni scientifiche. …”
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Functional analysis of a missense mutation in the serine protease inhibitor SPINT2 associated with congenital sodium diarrhea. by Nicolas Faller, Ivan Gautschi, Laurent Schild

“…We established a cellular assay in Xenopus laevis oocytes to study functional interactions between HAI-2 and candidate membrane-bound serine proteases expressed in the gastro-intestinal tract. …”
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Development of the retinotectal system in the direct-developing frog <it>Eleutherodactylus coqui </it>in comparison with other anurans by Schlosser Gerhard

“…</p> <p>Results</p> <p>Here, I compare growth and differentiation of the retina and tectum and development of early axon tracts in the brain between <it>Eleutherodactylus coqui </it>and the biphasically developing frogs <it>Discoglossus pictus</it>, <it>Physalaemus pustulosus</it>, and <it>Xenopus laevis </it>using morphometry, immunohistochemical detection of proliferating cell nuclear antigen (PCNA) and acetylated tubulin, biocytin tracing, and in situ hybridization for <it>NeuroD</it>. …”
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Functional Characterization of a Novel Class of Morantel-Sensitive Acetylcholine Receptors in Nematodes. by Elise Courtot, Claude L Charvet, Robin N Beech, Abdallah Harmache, Adrian J Wolstenholme, Lindy Holden-Dye, Vincent O'Connor, Nicolas Peineau, Debra J Woods, Cedric Neveu

“…We demonstrated that co-expression of Hco-ACR-26 and Hco-ACR-27 in Xenopus laevis oocytes led to the functional expression of an acetylcholine-receptor highly sensitive to the anthelmintics morantel and pyrantel. …”
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Trefoil Factor Family (TFF) Peptides by Werner Hoffmann

“…TFF peptides possess a single (mammalian TFF1 and TFF3), two (mammalian TFF2, <i>Xenopus laevis</i> xP2) or four TFF domains (<i>X. laevis</i> xP4). …”
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The POZ-ZF transcription factor Kaiso (ZBTB33) induces inflammation and progenitor cell differentiation in the murine intestine. by Roopali Chaudhary, Christina C Pierre, Kyster Nanan, Daria Wojtal, Simona Morone, Christopher Pinelli, Geoffrey A Wood, Sylvie Robine, Juliet M Daniel

“…However, most of the information regarding Kaiso's function to date has been gleaned from studies in Xenopus laevis embryos and mammalian cultured cells. To examine Kaiso's role in a relevant, mammalian organ-specific context, we generated and characterized a Kaiso transgenic mouse expressing a murine Kaiso transgene under the control of the intestine-specific villin promoter. …”
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Lack of negatively charged residues at the external mouth of Kir2.2 channels enable the voltage-dependent block by external Mg2+. by Junwei Li, Xiaoxiao Xie, Jun Liu, Hui Yu, Suhua Zhang, Yong Zhan, Hailin Zhang, Diomedes E Logothetis, Hailong An

“…Electrophysiological recordings on Kir2.2 and its mutants were performed by heterologous expression in Xenopus laevis oocytes. Our results confirmed that extracellular Mg2+ could reduce heterologously expressed WT Kir2.2 currents in a voltage dependent manner. …”
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Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels. by Maria A Tejada, Kathleen Stople, Sofia Hammami Bomholtz, Anne-Kristine Meinild, Asser Nyander Poulsen, Dan A Klaerke

“…For this purpose Slick and Slack channels were co-expressed with aquaporin 1 in Xenopus laevis oocytes and cell volume changes of around 5% were induced by exposure to hypotonic or hypertonic media. …”
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The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility by Laura Fin, Giorgia Bergamin, Roberto A. Steiner, Simon M. Hughes

“…Functional assays in mouse show cannabinoid sensitivity changes and Cnrip1 has recently been suggested to control eye development in Xenopus laevis. Two Cnrip1 genes are described in zebrafish, cnrip1a and cnrip1b. …”
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Antimicrobial peptides: The natural proteins and the future application for treatment of infectious diseases by Sorasak Intorasoot

“…Cathelicidins, defensins and histatins are human short polypeptides that mostly found in various kinds of cells, tissues and body fluids whereas protegrins and magainins are synthesized in porcine leukocytes and skin of Xenopus laevis, an African clawed frog, respectively. …”
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