Correlated Disorder in Myelinated Axons Orientational Geometry and Structure by Michael Di Gioacchino, Gaetano Campi, Nicola Poccia, Antonio Bianconi

“…In this work, we investigated the fluctuation of the myelin supramolecular assembly by measuring the spatial distribution of orientation fluctuations of axons in a Xenopus Laevis sciatic nerve associated with nerve functionality. …”
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Inheritance of Histones H3 and H4 during DNA Replication In Vitro by Egbert Vincent Madamba, Ellora Bellows Berthet, Nicole Jane Francis

“…Keywords: epigenetic, histones, chromatin, DNA replication, SV40, Xenopus laevis…”
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Rapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice by Nobuo Kariyama, Hiromi Sakata-Haga, Tsuyoshi Tsukada, Hiroki Shimada, Makoto Taniguchi, Toshihisa Hatta

“…Abstract We developed a non-destructive and rapid whole-mount bone staining method for small fish, Xenopus laevis, and rodent fetuses (RAP-B). RAP-B does not require skin or soft tissue removal. …”
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γ-Aminobutyric acid transporter 2 mediates the hepatic uptake of guanidinoacetate, the creatine biosynthetic precursor, in rats. by Masanori Tachikawa, Saori Ikeda, Jun Fujinawa, Shirou Hirose, Shin-ichi Akanuma, Ken-ichi Hosoya

“…The characteristics of [(14)C]GAA uptake by hepatocytes were elucidated using the in vivo liver uptake method, freshly isolated rat hepatocytes, an expression system of Xenopus laevis oocytes, gene knockdown, and an immunohistochemical technique. …”
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The Natural Plant Product Rottlerin Activates Kv7.1/KCNE1 Channels by Veronika Matschke, Ilaria Piccini, Janina Schubert, Eva Wrobel, Florian Lang, Johann Matschke, Elsie Amedonu, Sven G. Meuth, Timo Strünker, Nathalie Strutz-Seebohm, Boris Greber, Jürgen Scherkenbeck, Guiscard Seebohm

“…Methods: Here, we show the modulation of Kv7 channels by the natural compound Rottlerin heterologously expressed in Xenopus laevis oocytes and on iPSC cardiomyocytes overexpressing Kv7.1 channels. …”
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Reversal of DDK-Mediated MCM Phosphorylation by Rif1-PP1 Regulates Replication Initiation and Replisome Stability Independently of ATR/Chk1 by Robert C. Alver, Gaganmeet Singh Chadha, Peter J. Gillespie, J. Julian Blow

“…We show that, in Xenopus laevis egg extracts and human cells, hyper-phosphorylation of DNA-bound Mcm4, but not phosphorylation of Mcm2, correlates with DNA replication. …”
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Interaction of Excitatory Amino Acid Transporters 1 – 3 (EAAT1, EAAT2, EAAT3) with N-Carbamoylglutamate and N-Acetylglutamate by Birgitta C. Burckhardt, Gerhard Burckhardt

“…Due to structural similarities to glutamate, NCG may be absorbed in the intestine and taken up into the liver by excitatory amino acid transporters (EAATs). Methods: Using Xenopus laevis oocytes expressing either human EAAT1, 2, or 3, or human sodium-dependent dicarboxylate transporter 3 (NaDC3), transport-associated currents of NAG, NCG, and related dicarboxylates were assayed. …”
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Coordinated action of multiple transporters in the acquisition of essential cationic amino acids by the intracellular parasite Toxoplasma gondii. by Stephen J Fairweather, Esther Rajendran, Martin Blume, Kiran Javed, Birte Steinhöfel, Malcolm J McConville, Kiaran Kirk, Stefan Bröer, Giel G van Dooren

“…Using a combination of parasite assays and heterologous characterisation of TgApiAT6-1 in Xenopus laevis oocytes, we demonstrate that TgApiAT6-1 is a general cationic amino acid transporter that mediates both the high-affinity uptake of lysine and the low-affinity uptake of arginine. …”
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Pressure-selective modulation of NMDA receptor subtypes may reflect 3D structural differences by Amir eMor, Yosef Yehuda Kuttner, Shiri eLevy, Mor eMerav, Michael eHollmann, Yoram eGrossman

“…GluN1(1a or 1b) was co-expressed with one of the four GluN2(A-D) subunits in Xenopus laevis oocytes. HP increased ionic currents (measured by two electrode voltage clamps) of one subtype, reduced the current in four others, and did not affect the current in the remaining three. 3D theoretical modeling was aimed at revealing specific receptor domains involved with HP selectivity. …”
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Novel dicarboxylate selectivity in an insect glutamate transporter homolog. by Hui Wang, Avi M Rascoe, David C Holley, Eric Gouaux, Michael P Kavanaugh

“…Functional analysis of engineered mutant mosquito and human transporters expressed in Xenopus laevis oocytes provide support for a model defining interactions of charged and polar transporter residues in TMD8 with α-amino acids and ions. …”
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Transcriptome of Xenopus andrei, an octoploid frog, during embryonic development by Mark E. Pownall, Ronald R. Cutler, Margaret S. Saha

“…However, transcriptomic information is currently available for only one of these species, the tetraploid Xenopus laevis. Xenopus andrei, an octoploid species within the Xenopus genus, offers an opportunity for assessing a novel polyploid transcriptome during vertebrate development. …”
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Sodium-calcium exchanger mediates sensory-evoked glial calcium transients in the developing retinotectal system by Nicholas J. Benfey, Vanessa J. Li, Anne Schohl, Edward S. Ruthazer

“…During early development of the Xenopus laevis visual system, radial astrocytes in the optic tectum are highly responsive to sensory stimulation. …”
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Regulation of ALF promoter activity in Xenopus oocytes. by Dan Li, Abbas Raza, Jeff DeJong

“…BACKGROUND:In this report we evaluate the use of Xenopus laevis oocytes as a matched germ cell system for characterizing the organization and transcriptional activity of a germ cell-specific X. laevis promoter. …”
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Exposure Impacts of Diquat dibromide herbicide formulation on amphibian larval development by Oluwaseun Olusegun Babalola, Hannes Johannes van Wyk

“…Using Xenopus Metamorphosis Assay (XEMA) protocol, this study assessed the thyroidal effects of environmentally relevant concentrations of Diquat dibromide at 0.05, 0.11, and 0.14 mg/L on Xenopus laevis metamorphosis. The formulation significantly reduced both the fore and hind limb lengths, and disrupted the developmental stage at concentrations of 0.11 and 0.14 mg/L, with a median at NF-stage 57, while median of NF-stage 60 was recorded in the control. …”
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Embryos assist morphogenesis of others through calcium and ATP signaling mechanisms in collective teratogen resistance by Angela Tung, Megan M. Sperry, Wesley Clawson, Ananya Pavuluri, Sydney Bulatao, Michelle Yue, Ramses Martinez Flores, Vaibhav P. Pai, Patrick McMillen, Franz Kuchling, Michael Levin

“…Here, we show that Xenopus laevis embryos are much more sensitive to a diverse set of chemical and molecular-biological perturbations when allowed to develop alone or in small groups, than in large groups. …”
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Do Amino Acid Antiporters Have Asymmetric Substrate Specificity? by Gregory Gauthier-Coles, Stephen J. Fairweather, Angelika Bröer, Stefan Bröer

“…Here we used LC–MS to detect the movement of ¹²C- and ¹³C-labelled amino acid mixtures across the plasma membrane of <i>Xenopus laevis</i> oocytes expressing a variety of amino acid antiporters. …”
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Transmembrane voltage potential is an essential cellular parameter for the detection and control of tumor development in a Xenopus model by Brook T. Chernet, Michael Levin

“…We tested the role of transmembrane potential in tumorigenesis mediated by canonical oncogenes in Xenopus laevis. Depolarized membrane potential (Vmem) was a characteristic of induced tumor-like structures (ITLSs) generated by overexpression of Gli1, KrasG12D, Xrel3 or p53Trp248. …”
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Expression of Wnt signaling components during Xenopus pronephros development. by Bo Zhang, Uyen Tran, Oliver Wessely

“…While some individual Wnt signaling components have been studied extensively in the kidney, the overall pathway has not yet been analyzed in depth.Here we report a detailed expression analysis of all Wnt ligands, receptors and several downstream Wnt effectors during pronephros development in Xenopus laevis using in situ hybridization. Out of 19 Wnt ligands, only three, Wnt4, Wnt9a and Wnt11, are specifically expressed in the pronephros. …”
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A fungal metabolite asperparaline a strongly and selectively blocks insect nicotinic acetylcholine receptors: the first report on the mode of action. by Koichi Hirata, Saori Kataoka, Shogo Furutani, Hideo Hayashi, Kazuhiko Matsuda

“…In contrast to showing marked blocking action on the insect nAChRs, it exhibited only a weak blocking action on chicken α3β4, α4β2 and α7 nAChRs expressed in Xenopus laevis oocytes, suggesting a high selectivity for insect over certain vertebrate nAChRs.…”
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Biochemical evidence that the whole compartment activity behavior of GAPDH differs between the cytoplasm and nucleus by Helen S. Tang, Chelsea R. Gates, Michael C. Schultz

“…Homogenates of pristine nuclei and cytoplasms isolated from Xenopus laevis oocytes were used for whole compartment activity profiling in a near-physiological buffer. …”
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