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Development of selectable markers for mitochondrial transformation in yeast
Published 2024“…This study aimed to explore the feasibility of employing these AbR markers for selecting transformed yeast cells. Additionally, the usefulness of these AbR genes as selectable markers for yeast mitochondrial transformation was assessed by fusing a mitochondrial targeting signal (MTS) to the N-terminus of these genes using overlapping PCR. …”
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Yeast-laden Hydrogel Capsules for Scalable Trace Lead Removal from Water
Published 2024“…The yeast-laden capsules exhibit an uptake capacity of 21 mg g−1, comparable to free yeast under the same conditions, reaching equilibrium within the first 5 minutes of contact. …”
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Population Balance Model-based Dynamic Multiobjective Optimization of Yeast Cell Manufacturing
Published 2024“…We investigate a continuous yeast cell manufacturing process that produces sustained oscillations in outputs under nominal conditions. …”
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Insight into actin organization and function in cytokinesis from analysis of fission yeast mutants
Published 2014“…The actin cytoskeleton of the fission yeast, Schizosaccharomyces pombe, has been extensively characterized and contains structures analogous to those in other cell types. …”
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Journal Article -
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Exploring the potential of Nyonya Kuih residues as the substrate for yeast cell protein production
Published 2024“…Despite the yeast cells’ yield (by dry cell weight) from molasses fermentation being 19% and 11% higher than those from KT and KL hydrolysates, respectively, KT and KL hydrolysates were proven superior due to the significantly higher (p<0.05) crude protein content in yeast cell produced from both hydrolysates. …”
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The yeast FIT2 homologs are necessary to maintain cellular proteostasis and membrane lipid homeostasis
Published 2022“…While LD biogenesis and basal endoplasmic reticulum (ER) stress-induced unfolded protein response (UPR) remain unaltered in ScFIT mutants, SCS3 was found to be essential for proper stress-induced UPR activation and for viability in the absence of the sole yeast UPR transducer IRE1 Owing to not having a functional UPR, cells with mutated SCS3 exhibited an accumulation of triacylglycerol within the ER along with aberrant LD morphology, suggesting that there is a UPR-dependent compensatory mechanism that acts to mitigate lack of SCS3 Additionally, SCS3 was necessary to maintain phospholipid homeostasis. …”
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Journal Article -
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Yeast9: a consensus genome-scale metabolic model for S. cerevisiae curated by the community
Published 2024“…Genome-scale metabolic models (GEMs) can facilitate metabolism-focused multi-omics integrative analysis. Since Yeast8, the yeast-GEM of Saccharomyces cerevisiae, published in 2019, has been continuously updated by the community. …”
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Genetic, genomic and systems biology analysis of adaptive evolution of budding yeast to the deletion of essential genes
Published 2016“…However previous studies in budding yeast challenged this view by showing that mutants deleted of essential or non-essential genes are capable of accumulating compensatory mutations and resume viability, suggesting that essentiality is not an intrinsic gene property but depends on the cellular capacity to evolve. …”
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Research Report -
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Identifying antifungal resistance mechanisms and modes of action using SAturated Transposon Analysis in Yeast (SATAY)
Published 2024Subjects:Thesis -
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Isolation, identification, and characteristics of local yeast isolates with proteolytic capability obtained from traditional processed Indonesian duck eggs
Published 2024“…Various microorganisms can grow in egg-based food products, and yeast is among the microorganisms that thrive in egg products. …”
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A yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein–protein interactions identifies a novel putative Mdm2-binding site in p53
Published 2018Subjects: “…Yeast Two-hybrid Assay…”
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Journal Article -
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Caspase interaction of anti-apoptotic Livin as well as the Vacuolar ATPase (V-ATPase) and structural insights into the subunit d and a of the yeast V-ATPase
Published 2009“…Additionally, homogenous protein preparation of yeast subunit d protein was used to determine its first low resolution shape by small angle X-ray spectroscopy (SAXS), revealing two distinct domains of 6.5 nm and 3.5 nm widths forming a “boxing glove” shape. …”
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Thesis -
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Cytotoxicity assessment of α helix Antarctic yeast oriented antifreeze peptide (Afp1m) on M. dunni (Clone III8C) cells
Published 2024“…In order to assess the cytotoxic effects of the cryoprotectant helix Antarctic yeast-orientated antifreeze peptide Afp1m on normal mouse skin fibroblasts, an in vitro model was developed for cytotoxicity assessment. …”
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Profiling the growth conditions and persistent organic pollutants (POPS) tolerance of Phenoliferia glacialis USM-PSY62
Published 2024“…USM-PSY62 is a psychrophilic yeast isolated from the ice brine of Antarctica. …”
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Molecular, Genetic, and Process Approaches for Improving Secreted Pharmaceutical Protein Quality in Komagataella phaffii
Published 2024“…We further showed the comparability between yeast- and CHO-secreted products, providing a framework for rapid product development with this unconventional yeast. …”
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Thesis -
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Identifying the interaction between N-WASP, an actin nucleation factor and vinculin, a focal adhesion protein
Published 2015“…Although the two-hybrid assay showed interaction between N-WASP and vinculin in yeast cells, vinculin could not be detected in the pull down. …”
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Final Year Project (FYP) -
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Screening of microbial population in Sabah tea kombucha pellicle for its potential as prebiotic and probiotic supplement
Published 2024“…This research aimed to determine and analyze the microbial population in kombucha pellicles derived from Sabah black tea, specifically focusing on bacteria and yeast, to gain insights into their abundance, diversity, and potential as prebiotic and probiotic supplements. …”
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Article