Structural Motifs and Spatial Structures of Helicase (NS3) and RNA-dependent RNA-polymerase (NS5) of a Flavi-like Kindia tick virus (unclassified Flaviviridae) by A. A. Gladysheva, A. V. Gladysheva, V. A. Ternovoi, V. B. Loktev

“…Also, eight amino acid substitutions characteristic of KITV/2018/1 and KITV/2018/2 were detected, five of them being localized in alpha-helix and three in loops of nonstructural proteins. …”
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Identification of the divergent calmodulin binding motif in yeast Ssb1/Hsp75 protein and in other HSP70 family members by R.C. Heinen, L. Diniz-Mendes, J.T. Silva, V.M.F. Paschoalin

“…Computational analysis of the primary structure of Ssb1/Hsp75 identified a unique potential amphipathic alpha-helix in its N-terminal ATPase domain with features of target regions for Ca2+/calmodulin binding. …”
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Studies of alpha-helicity and intersegmental interactions in voltage-gated Na+ channels: S2D4. by Zhongming Ma, Jun Kong, Roland G Kallen

“…The results of cysteine substitution at the two most sensitive sites of the S2D4 alpha-helix (N1382 and E1392C) support the existence of electrostatic network interactions between S2 and other transmembrane segments within Na(v)1.4D4 similar to but not identical to those proposed for K+ channels.…”
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Prediction of an Efficient Signal Peptide for Optimized Expression of Mycobacterium Tuberculosis Heparin-binding Hemagglutinin Gene in Periplasmic Compartment of Escherichia Coli:... by Ali Forouharmehr, Nemat Shams, Narges Nazifi, Amin Jaydari, Ehsan Rashidian

“…Moreover, the results showed that the secondary structure of the adjuvant linked to asr peptide contained 88.18% alpha helix and 9.5% random coil. Also, the results of in silico cloning showed that the nucleotide sequences of the adjuvant linked to the asr peptide were successfully cloned between BamHI and XhoI enzymes in the multiple cloning site of pET21a (+). …”
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Computational biophysical characterization of the SARS-CoV-2 spike protein binding with the ACE2 receptor and implications for infectivity by Ratul Chowdhury, Veda Sheersh Boorla, Costas D. Maranas

“…Strong binding is mediated through strong electrostatic attachments with every fourth residue on the N-terminus alpha-helix (starting from Ser19 to Asn53) as the turn of the helix makes these residues solvent accessible. …”
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E2F1 binds to the peptide-binding groove within the BIR3 domain of cIAP1 and requires cIAP1 for chromatin binding. by Jennifer Allègre, Jessy Cartier, Valérie Glorian, Nathalie Droin, Baptiste Dumetier, Cémile Kayaci, Jean Berthelet, Simon Gemble, Céline Vuillier, Laurent Maillet, Carmen Garrido, Laurence Dubrez

“…We demonstrated that the first alpha-helix of BIR3 was required for E2F1 binding, as well as for the binding of Smac and Smac mimetics. …”
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Human lysosomal acid lipase/cholesteryl ester hydrolase and human gastric lipase: site-directed mutagenesis of Cys227 and Cys236 results in substrate-dependent reduction of enzymat... by P Lohse, P Lohse, S Chahrokh-Zadeh, D Seidel

“…Deletion of the eight-amino acid alpha-helix as well as disruption of its hydrophobic surface, in contrast, abolished enzymatic activity. …”
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Conformationally Restricted Peptides from Rice Proteins Elicit Antibodies That Recognize the Corresponding Native Protein in ELISA Assays by Nubia F. Barrera, Luz M. Melgarejo, Maribel Cruz-Gallego, Lina Jimena Cortés, Fanny Guzmán, Julio C. Calvo

“…In another test, peptide dendrimers containing sequences conformationally restricted to α-helix from four of those rice proteins were synthesized. …”
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Structure and non-structure of centrosomal proteins. by Helena G Dos Santos, David Abia, Robert Janowski, Gulnahar Mortuza, Michela G Bertero, Maïlys Boutin, Nayibe Guarín, Raúl Méndez-Giraldez, Alfonso Nuñez, Juan G Pedrero, Pilar Redondo, María Sanz, Silvia Speroni, Florian Teichert, Marta Bruix, José M Carazo, Cayetano Gonzalez, José Reina, José M Valpuesta, Isabelle Vernos, Juan C Zabala, Guillermo Montoya, Miquel Coll, Ugo Bastolla, Luis Serrano

“…This difference is mainly due to residues predicted as disordered and helical (30% in centrosomal and 9.4% in control proteins), which may correspond to alpha-helix forming molecular recognition features (α-MoRFs). …”
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Generation of dimeric single-chain antibodies neutralizing the cytolytic activity of vaginolysin by Zilvinas Dapkunas, Aurimas Baranauskas, Gitana Mickiene, Milda Pleckaityte, Gintautas Zvirblis

“…To improve the properties of scFvs, monospecific dimeric scFvs were generated by the genetic fusion of two anti-VLY scFv molecules connected by an alpha-helix-forming peptide linker. Results: N-terminal hexahistidine-tagged dimeric scFvs were constructed and produced in Escherichia coli and purified using metal chelate affinity chromatography. …”
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Insights on the structure and stability of Licanantase: a trimeric acid-stable coiled-coil lipoprotein from Acidithiobacillus thiooxidans by Fernando Abarca, Sebastian E. Gutierrez-Maldonado, Pilar Parada, Patricio Martinez, Alejandro Maass, Tomas Perez-Acle

“…Lic and Lpp share the secretion motif, intracellular processing and alpha helix structure, as well as the distribution of hydrophobic residues in heptads forming a hydrophobic core, typical of coiled-coil structures. …”
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A Conserved Tryptophan in the Envelope Cytoplasmic Tail Regulates HIV-1 Assembly and Spread by Xenia Snetkov, Tafhima Haider, Dejan Mesner, Nicholas Groves, Schuyler B. van Engelenburg, Clare Jolly

“…Here, we identified a highly conserved tryptophan motif at position 757 (W757) in the LLP-2 alpha helix of the EnvCT as a key determinant for HIV-1 replication and spread between T cells. …”
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Bioinformatics analysis of 4Hppd gene in Sporothrix schenckii by Xiao-yan WU, Jing ZHANG, Jia-min HUANG, Yao SUN, Xiao-wen HUANG, Wen-chao GAO, Rong WU, San-mei CHEN, Huai-qiu HUANG

“…The dominant secondary structures in Ss.4HppD were random coil and alpha helix. Besides, Ss.4HppD had a similar tertiary structure with its homologue proteins, a glyoxalase motif at the carboxy end and multiple phosphorylation sites. …”
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Identification of B cell epitopes of alcohol dehydrogenase allergen of Curvularia lunata. by Smitha Nair, Neetu Kukreja, Bhanu Pratap Singh, Naveen Arora

“…RESULT: The homology model showed GroES like catalytic domain joined to Rossmann superfamily domain by an alpha helix. Stereochemical quality was confirmed by Procheck which showed 90% residues in most favorable region of Ramachandran plot while Errat gave a quality score of 92.733%. …”
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Effects of an Electric Field on the Conformational Transition of the Protein: Pulsed and Oscillating Electric Fields with Different Frequencies by Qun Zhang, Dongqing Shao, Peng Xu, Zhouting Jiang

“…Concerning the typical sample 1BBL, the secondary structure analysis showed that two alpha-helix segments both transit to turns or random coils almost simultaneously when it is exposed in a pulsed electric field. …”
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Phylogenetic Analysis and In Silico Characterization of Cytochrome P450 1A (Cyp1A) Protein from the African Catfish, Clarias gariepinus (Burchell, 1822) by Muthuramalingam KARPAGAVALLI, Muthusamy THANGARAJ, Duraisamy ANNADURAI, Thangappan AJITHKUMAR, Mahapathra GYANAPRAKASH, Raman SURABI

“…The prediction results depicted that most of the amino acids formed alpha helix. The predicted pI was 9.10, hydropathycity was -0.226, exposed and buried residues were 61.67, 38.33% respectively. …”
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Development of a Novel Multi-Epitope Vaccine Candidate against Streptococcus Iniae Infection in Fish: An Immunoinformatics Study by Ali Forouharmehr, A Banan, S. M Mousavi, A Jaydari

“…Furthermore, the structure analysis results revealed that the designed vaccine contained 23.49% alpha helix, with 90.5% residues in favored region. …”
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Analysis of ORF5 protein signifies its importance in Norway rat Hepatitis E virus by Zoya Shafat, Anwar Ahmed, Mohammad K. Parvez, et al.

“…The secondary structure of ORF5 protein showed all three major components (alpha-helix, beta-strand and random coil). The tertiary structure generated through homology modelling revealed mixed 汐/汕 structural fold with subsequently higher percentage of strands and abundance of coils. …”
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Recognition of <i>Aedes aegypti</i> Mosquito Saliva Protein LTRIN by the Human Receptor LTβR for Controlling the Immune Response by Su Ning Loh, Ian Russell Anthony, Edem Gavor, Xin Shan Lim, R. Manjunatha Kini, Yu Keung Mok, J. Sivaraman

“…We demonstrated that the truncated C-terminal LTRIN (ΔLTRIN) is a dimeric protein with a stable alpha helix-dominant secondary structure, which possibly aids in withstanding the temperature fluctuations during blood-feeding events. …”
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Calcium binding promotes prion protein fragment 90-231 conformational change toward a membrane destabilizing and cytotoxic structure. by Sacha Sorrentino, Tonino Bucciarelli, Alessandro Corsaro, Alessio Tosatto, Stefano Thellung, Valentina Villa, M Eugenia Schininà, Bruno Maras, Roberta Galeno, Luca Scotti, Francesco Creati, Alessandro Marrone, Nazzareno Re, Antonio Aceto, Tullio Florio, Michele Mazzanti

“…We also report that Ca⁺⁺ binding to hPrP₉₀₋₂₃₁ induces a conformational change based on an alteration of secondary structure characterized by loss of alpha-helix content causing hydrophobic amino acid exposure and proteinase K resistance. …”
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