Thermal Inactivation Mechanism and Structural Features Providing Enhanced Thermal Stability of Hyperthermophilic <i>Thermococcus sibiricus</i> L-Asparaginase in Comparison with Mes... by Natalia Dobryakova, Dmitry Zhdanov, Maria Dumina, Svetlana Aleksandrova, Marina Pokrovskaya, Alexander Genin, Anastasia Shishparenok, Alexander Zhgun, Elena Vadimovna Kudryashova

“…This work aimed to study the structural features and mechanisms of thermoinactivation of hyperthermophilic L-asparaginase (L-ASNase) from archaea <i>Thermococcus sibiricus</i> (TsA) in comparison with bacterial L-ASNases from <i>Melioribacter roseus</i> (MrA) and <i>Rhodospirillum rubrum</i> (RrA). …”
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Alterations in the Rumen Liquid-, Particle- and Epithelium-Associated Microbiota of Dairy Cows during the Transition from a Silage- and Concentrate-Based Ration to Pasture in Sprin... by Ulrich Meyer, Melanie Schären, Kerstin Kiri, Susanne Riede, Mark Gardener, Jürgen Hummel, Tim Urich, Gerhard Breves, Sven Dänicke

“…This study aims to investigate how the rumen liquid (LAAB), particle (PAAB), and epithelium (EAAB) associated archaea and bacteria are influenced by this nutritional change. …”
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Harnessing a methane-fueled, sediment-free mixed microbial community for utilization of distributed sources of natural gas by Marlow, Jeffrey J., Kumar, Amit, Enalls, Brandon C., Reynard, Linda M., Tuross, Noreen, Stephanopoulos, Gregory, Girguis, Peter

“…Methane is particularly promising because it is abundant and energy-rich, yet the most efficient methane-activating metabolic pathways involve mixed communities of anaerobic methanotrophic archaea and sulfate reducing bacteria. These communities oxidize methane at high catabolic efficiency and produce chemically reduced by-products at a comparable rate and in near-stoichiometric proportion to methane consumption. …”
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Structural dynamics of community gene expression In a freshwater cyanobacterial bloom over a day-night cycle by Wang, Jia, Ph. D. Massachusetts Institute of Technology

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Stable isotope labeling confirms mixotrophic nature of streamer biofilm communities at alkaline hot springs by Schubotz, Florence, Hays, Lindsay E., Meyer-Dombard, D'Arcy R., Gillespie, Aimee, Shock, Everett L., Summons, Roger E

“…Nearest the hydrothermal source (75–88°C) SBC comprise thermophilic Archaea and Bacteria, often mixed communities including Desulfurococcales and uncultured Crenarchaeota, as well as Aquificae and Thermus, each carrying diagnostic membrane lipid biomarkers. …”
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A Chemically Competent Thiosulfuranyl Radical on the Escherichia coli Class III Ribonucleotide Reductase by Wei, Yifeng, Mathies, Guinevere, Yokoyama, Kenichi, Chen, Jiahao, Stubbe, JoAnne, Griffin, Robert Guy

“…The class III ribonucleotide reductases (RNRs) are glycyl radical (G•) enzymes that provide the balanced pool of deoxynucleotides required for DNA synthesis and repair in many facultative and obligate anaerobic bacteria and archaea. Unlike the class I and II RNRs, where reducing equivalents for the reaction are delivered by a redoxin (thioredoxin, glutaredoxin, or NrdH) via a pair of conserved active site cysteines, the class III RNRs examined to date use formate as the reductant. …”
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Identification and characterisation of thiamine pyrophosphate riboswitch in oil palm (Elaeis guineensis Jacq.) by Subki, Atiqah

“…TPP binds specifically to TPP riboswitch to regulate thiamine biosynthesis through a variety of mechanisms and they have been found in archaea, bacteria and eukaryotes. This study was carried out to hunt for TPP riboswitch in oil palm’s thiamine biosynthesis gene. …”
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Plants against cancer: the immune-boosting herbal microbiome: not of the plant, but in the plant. Basic concepts, introduction, and future resource for vaccine adjuvant discovery by Elizabeth Mazzio, Andrew Barnes, Ramesh Badisa, Stevie Council, Karam F. A. Soliman

“…The presence of microorganism communities (MOCs) comprised of bacteria, fungi, archaea, algae, protozoa, viruses, and the like, are ubiquitous in all living tissue, including plant and animal. …”
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Changes in the activities of key enzymes and the abundance of functional genes involved in nitrogen transformation in rice rhizosphere soil under different aerated conditions by Chun-mei XU, De-shun XIAO, CHEN Song, CHU Guang, LIU Yuan-hui, ZHANG Xiu-fu, WANG Dan-ying

“…The abundances of amoA ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), nirS, nirK, and nifH genes, and the activities of urease, protease, ammonia oxidase, nitrate reductase, and nitrite reductase were measured at the tillering (S1), heading (S2), and ripening (S3) stages. …”
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In situ community transcriptomics illuminates CO2-fixation potentials and supporting roles of phagotrophy and proton pump in plankton in a subtropical marginal sea by Hongfei Li, Jianwei Chen, Liying Yu, Guangyi Fan, Tangcheng Li, Ling Li, Huatao Yuan, Jingtian Wang, Cong Wang, Denghui Li, Senjie Lin

“…Phytoplankton and cyanobacteria fix CO2 to produce organic compounds using solar energy and mainly by the Calvin cycle, whereas autotrophic bacteria and archaea may fix CO2 by non-Calvin cycle carbon fixation pathways. …”
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Relationship between Nitrogen Dynamics and Key Microbial Nitrogen-Cycling Genes in an Intensive Freshwater Aquaculture Pond by Yifeng Yan, Junbo Zhou, Chenghao Du, Qian Yang, Jinhe Huang, Zhaolei Wang, Jun Xu, Min Zhang

“…Ammonia-oxidizing bacteria (AOB) were found to dominate the ammonia oxidation process over ammonia-oxidizing archaea (AOA) in both water and sediment. Redundancy analysis revealed a positive correlation between SPM flux, Chlorophyll a (Chl-a), and denitrification genes in the water, and between nitrogen-cycling genes and NH₄⁺/NO₂⁻ fluxes at the interface. …”
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Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery by Birthe Meineke, Johannes Heimgärtner, Rozina Caridha, Matthias F. Block, Kyle J. Kimler, Maria F. Pires, Michael Landreh, Simon J. Elsässer

“…Pyrrolysine tRNA (tRNAPyl)/pyrrolysyl-tRNA synthetase (PylRS) pairs from methanogenic archaea and engineered bacterial tRNA/aminoacyl-tRNA synthetase (aaRS) pairs are used for site-specific incorporation of non-canonical amino acids (ncAAs) in response to stop codons in mammalian cells. …”
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The ancient Virus World and evolution of cells by Dolja Valerian V, Senkevich Tatiana G, Koonin Eugene V

“…Somewhat paradoxically, under this scenario, we surmise that selfish genetic elements ancestral to viruses evolved prior to typical cells, to become intracellular parasites once bacteria and archaea arrived at the scene. Selection against excessively aggressive parasites that would kill off the host ensembles of genetic elements would lead to early evolution of temperate virus-like agents and primitive defense mechanisms, possibly, based on the RNA interference principle. …”
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Advancing environmental sustainability through microbial reprogramming in growth improvement, stress alleviation, and phytoremediation by Babar Iqbal, Guanlin Li, Khulood Fahad Alabbosh, Hamad Hussain, Ismail Khan, Muhammad Tariq, Qaiser Javed, Muhammad Naeem, Naveed Ahmad

“…Microorganisms encompassing bacteria, fungi, archaea, and viruses, have demonstrated their knack for stirring up growth-enabling hormones, activating pathways tuned to stress, and ameliorating the availability of nutrients by means of fixation and solubilization Furthermore, such microorganisms also display immense potential in phytoremediation strategies by aiding plants in the extraction, alteration, and detoxification of contaminants found in both soil and water. …”
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Distribution of Genes and Microbial Taxa Related to Soil Phosphorus Cycling across Soil Depths in Subtropical Forests by Hao Lv, Jie Yang, Siwen Su, Yue Liu, Jie Feng, Yuxiang Sheng, Ting Wang, Jinwen Pan, Li Tang, Liang Chen, Shuai Ouyang, Guangjun Wang

“…The four bacteria phyla that related to P cycling, <i>Proteobacteria</i>, <i>Actinobacteria</i>, <i>Bacteroidetes</i>, and <i>Candidatus</i>_<i>Eremiobacteraeota</i> were higher in organic soil; conversely, the three bacteria phyla (<i>Acidobacteria</i>, <i>Verrucomicrobia</i>, and <i>Chloroflexi</i>) and archaea taxa were more abundant in mineral soil. Therefore, we concluded that the distribution of genes and microbial taxa involved in soil P cycling differed among soil depths, providing a depth-resolved scale insight into the underlying mechanisms of P cycling by soil microorganisms in subtropical forests.…”
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The Action Potential of Antioxidant Grape Seed Proanthocyanidin as a Rumen Modifier to Mitigate Rumen Methanogenesis In Vitro by Fan Zhang, Zhenwei Zhang, Ya Wen, Qichao Wu, Luotong Zhang, Shengli Li, HongJian Yang

“…In addition, the GSP addition significantly decreased the ratio of methanogens to total bacteria (<i>p</i> < 0.05), and the reduction was notably greater in the HY than in the LY substrate (45.3% vs. 15.2% decrease), although the diversity of rumen methanogenic archaea was not affected in either the HY or the LY group. …”
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Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from <i>Agromyces indicus</i>: Enhanced Production, Characterization, and Optimization by Mohd Adnan, Arif Jamal Siddiqui, Syed Amir Ashraf, Mejdi Snoussi, Riadh Badraoui, Mousa Alreshidi, Abdelbaset Mohamed Elasbali, Waleed Abu Al-Soud, Salem Hussain Alharethi, Manojkumar Sachidanandan, Mitesh Patel

“…Polyhydroxybutyrate (PHB) is a biodegradable polymer that is produced by bacteria and archaea as carbon and energy reserves. Due to its rapid degradation in natural environments, it can be considered a biodegradable plastic alternative. …”
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Molecular evidence for stimulation of methane oxidation in Amazonian floodplains by ammonia-oxidizing communities by Gabriel G. T. N. Monteiro, Dayane J. Barros, Gabriele V. M. Gabriel, Andressa M. Venturini, Andressa M. Venturini, Tomás G. R. Veloso, Gisele H. Vazquez, Luciana C. Oliveira, Vania Neu, Paul L. E. Bodelier, Cleber Fernando M. Mansano, Siu M. Tsai, Acacio A. Navarrete, Acacio A. Navarrete

“…We found that ammonia-oxidizing archaea (AOA) were more abundant than ammonia-oxidizing bacteria (AOB) during both flood and non-flood seasons. …”
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A translation proofreader of archaeal origin imparts multi-aldehyde stress tolerance to land plants by Pradeep Kumar, Ankit Roy, Shivapura Jagadeesha Mukul, Avinash Kumar Singh, Dipesh Kumar Singh, Aswan Nalli, Pujaita Banerjee, Kandhalu Sagadevan Dinesh Babu, Bakthisaran Raman, Shobha P Kruparani, Imran Siddiqi, Rajan Sankaranarayanan

“…Extensive biochemical analysis revealed that only archaeal DTD2 can remove the stable D-aminoacyl adducts on tRNA thereby shielding archaea and plants from these system-generated aldehydes. …”
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Temporal order of evolution of DNA replication systems inferred by comparison of cellular and viral DNA polymerases by Koonin Eugene V

“…In particular, and counter-intuitively, given the central role of DNA in all cells and the mechanistic uniformity of replication, the core enzymes of the replication systems of bacteria and archaea (as well as eukaryotes) are unrelated or extremely distantly related. …”
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