Genetic regulation of Caenorhabditis elegans lysosome related organelle function. by Alexander A Soukas, Christopher E Carr, Gary Ruvkun

“…Caenorhabditis elegans LROs are the site of concentration of vital dyes such as Nile red as well as age-associated autofluorescence. Even though certain short-lived mutants have high LRO Nile red and high autofluorescence, and other long-lived mutants have low LRO Nile red and low autofluorescence, these two biologies are distinct. …”
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3D Printed Monolithic Microreactors for Real-Time Detection of <em>Klebsiella pneumoniae</em> and the Resistance Gene <em>bla</em>_NDM-1 by Recombinase Polymerase Amplifi... by Ole Behrmann, Matthias Hügle, Franz Eckardt, Iris Bachmann, Cecilia Heller, Marina Schramm, Carrie Turner, Frank T. Hufert, Gregory Dame

“…Both the general ability of the rtRPA reaction to occur while in contact with the cured 3D printing materials as well as the residual autofluorescence and fluorescence drift in dependence on post curing of the materials is characterized. …”
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Multi-pronged approach to human mesenchymal stromal cells senescence quantification with a focus on label-free methods by Weichao Zhai, Jerome Tan, Tobias Russell, Sixun Chen, Dennis McGonagle, May Win Naing, Derrick Yong, Elena Jones

“…Both the advanced methods demonstrated statistically significant values, (r = 0.76, p $$\le $$ ≤ 0.001 for the fluorogenic C $${_{12}}$$ 12 FDG method, and r = 0.72, p $$\le $$ ≤ 0.05 for the forward scatter method), and good fold difference ranges (1.120–4.436 for total autofluorescence mean and 1.082–6.362 for lipopigment autofluorescence mean) between early and senescent passage hMSCs. …”
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Bilateral pigmented paravenous chorioretinal atrophy in a patient with tuberculosis – A case report by Sanyukta Joshi, Mihir Trivedi, Chhaya Shinde

“…Here, we report a case of PPCRA in a young female with tuberculosis and fundus autofluorescence and ERG findings suggestive of macula involving bilateral PPCRA. …”
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Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiation by Laurence Dubreil, Noreddine Damane, Romain Fleurisson, Marine Charrier, Julien Pichon, Isabelle Leroux, Cindy Schleder, Mireille Ledevin, Thibaut Larcher, Frédéric Jamme, John Puentes, Karl Rouger

“…We investigated whether label-free tissue autofluorescence revealed by Synchrotron deep ultraviolet (DUV) radiation could serve as an additional tool for monitoring dystrophic muscle remodeling. …”
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Multicolour single molecule imaging in cells with near infra-red dyes. by Christopher J Tynan, David T Clarke, Benjamin C Coles, Daniel J Rolfe, Marisa L Martin-Fernandez, Stephen E D Webb

“…The autofluorescence background of biological samples impedes the detection of single molecules when imaging. …”
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Simultaneous Two-Photon Fluorescence Microscopy of NADH and FAD Using Pixel-to-Pixel Wavelength-Switching by Yifan Qin, Yuanqin Xia

“…In this paper, we demonstrate a new imaging system simultaneously capturing autofluorescence images from NADH and FAD, with high excitation efficiency and negligible spectral bleed-through. …”
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Human INCL fibroblasts display abnormal mitochondrial and lysosomal networks and heightened susceptibility to ROS-induced cell death. by Bailey Balouch, Halle Nagorsky, Truc Pham, James Thai LaGraff, Quynh Chu-LaGraff

“…In the absence of PPT1 activity, these proteins fail to be degraded, leading to the accumulation of autofluorescence storage material in the lysosome. The underlying molecular mechanisms leading to INCL pathology remain poorly understood. …”
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Brain BLAQ: Post-hoc thick-section histochemistry for localizing optogenetic constructs in neurons and their distal terminals by David Adam Kupferschmidt, Patrick A Cody, David M Lovinger, Margaret Isabella Davis

“…High-resolution imaging of thick, fixed brain sections would make such post-hoc assessment and association possible; however, thick sections often display autofluorescence that limits their compatibility with fluorescence microscopy. …”
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Three-Dimensional Superresolution Imaging of the FtsZ Ring during Cell Division of the Cyanobacterium <italic toggle="yes">Prochlorococcus</italic> by Riyue Liu, Yaxin Liu, Shichang Liu, Ying Wang, Kim Li, Ning Li, Daiying Xu, Qinglu Zeng

“…However, superresolution microscopy with lateral resolution better than 100 nm has not been achieved in photosynthetic cells due to the interference of a high-autofluorescence background. Here, we developed a photobleaching method to effectively reduce the autofluorescence of cyanobacterial and plant cells. …”
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Label-free optical biomarkers detect early calcific aortic valve disease in a wild-type mouse model by Ishita Tandon, Shelby Johns, Alan Woessner, Jessica Perez, Delaney Cross, Asya Ozkizilcik, Timothy J. Muldoon, Srikanth Vallurupalli, Muralidhar Padala, Kyle P. Quinn, Kartik Balachandran

“…Additionally, locations with lower TPEF autofluorescence ratios at 4 and 16 weeks exhibited increased calcification at later 28-week timepoints. …”
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An optimum study on the laser scanning confocal microscopy techniques for BiFC assay using plant protoplast by Jinhong Yuan, Daiyu Li, Yi Liang, Yao Meng, Li Li, Lin Yang, Mingyue Pei, Liuchun Feng, Junhua Li

“…Conclusion When the BiFC signals are weak, they may be affected by chloroplast autofluorescence. However, when used properly, the autofluorescence of chloroplasts can serve as an excellent internal marker for effectively distinguishing other signals. …”
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Multimodal Retinal Imaging of Intravascular Lipid in Severe/Extreme Hypertriglyceridemia by Christos Christakopoulos

“…Optical coherence tomography, fluorescein angiography, infrared fundus photography, and autofluorescence studies of the retina demonstrated unique findings. …”
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Outer retinal tubulation and inner retinal pseudocysts in a patient with maternally inherited diabetes and deafness evaluated with optical coherence tomography angiogram by Koushik Tripathy, Barsha Sarma, Shahana Mazumdar

“…A 47-year-old lady (index case) with diabetes and deafness showed multiple oval circumferential areas of perifoveal atrophy in both eyes. Autofluorescence revealed areas of hypoautofluorescence. …”
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Novel imaging techniques for hydroxychloroquine retinopathy by Yusuf, I, Charbel Issa, P, Seong Joon, A

“…In addition to conventional imaging modalities, such as spectral-domain optical coherence tomography (OCT) and fundus autofluorescence (FAF), novel retinal imaging techniques such as en face OCT, OCT angiography, fluorescence lifetime imaging ophthalmoscopy, quantitative autofluorescence, and retromode imaging are capable of detecting structural changes in the retina. …”

Fructosamine-3-Kinase as a Potential Treatment Option for Age-Related Macular Degeneration by Sander De Bruyne, Caroline Van den Broecke, Henk Vrielinck, Samira Khelifi, Olivier De Wever, Ken Bracke, Manon Huizing, Nezahat Boston, Jonas Himpe, Marijn Speeckaert, Anne Vral, Jo Van Dorpe, Elisabeth Van Aken, Joris R. Delanghe

“…FN3K treatment provoked a significant decrease (41%) of AGE-related autofluorescence in the AGE-modified porcine retinas. Furthermore, treatment of human neural retinas resulted in significant decreases of autofluorescence (−24%). …”
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SNR enhanced high-speed two-photon microscopy using a pulse picker and time gating detection by Jeonggeun Song, Juehyung Kang, Ungyo Kang, Hyeong Soo Nam, Hyun Jung Kim, Ryeong Hyeon Kim, Jin Won Kim, Hongki Yoo

“…In particular, label-free autofluorescence imaging offers various advantages for imaging biological samples. …”
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The Attenuation of Insulin/IGF-1 Signaling Pathway Plays a Crucial Role in the Myo-Inositol-Alleviated Aging in <i>Caenorhabditis elegans</i> by Nae-Cherng Yang, Chia-Yu Chin, Ya-Xin Zheng, Inn Lee

“…The functions of the lifespan-extending, health-improving, and autofluorescence-decreasing effects of MI disappeared in the AKT-1 and DAF-16 mutants. …”
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Long-lived bright red emitting azaoxa-triangulenium fluorophores. by Badri P Maliwal, Rafal Fudala, Sangram Raut, Rutika Kokate, Thomas J Sørensen, Bo W Laursen, Zygmunt Gryczynski, Ignacy Gryczynski

“…In addition, the nanosecond background autofluorescence is a significant interference during fluorescence measurements in cellular environment. …”
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Myofibril Changes in the Copepod Pseudodiaptomus marinus Exposed to Haline and Thermal Stresses. by Ali Ibrahim, Anissa Souissi, Aymeric Leray, Laurent Héliot, Bernard Vandenbunder, Sami Souissi

“…Our results suggest the use of cuticular and muscle autofluorescence as new biomarkers of stress detectable in formalin-preserved P. marinus individuals. …”
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