Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing <i>Komagataella phaffii</i> Strains Utilizing Glycerol as Substrate by Nadielle Tamires Moreira Melo, Gabriela Coimbra Pontes, Dielle Pierotti Procópio, Gabriel Caetano de Gois e Cunha, Kevy Pontes Eliodório, Hugo Costa Paes, Thiago Olitta Basso, Nádia Skorupa Parachin

“…This study clarifies the underlying metabolic reasons for arabitol formation in <i>K. phaffii</i> and points to ways for improving production of lactic acid using <i>K. phaffii</i> as a biocatalyst.…”
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The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability by Jung-Hyun Ju, Sang-Gyu Jeon, Kyung Min Lee, Sun-Yeon Heo, Min-Soo Kim, Chul-Ho Kim, Baek-Rock Oh

“…We isolated <i>L. reuteri</i> BR201 as a biocatalyst for 3-HPA production and confirmed the effect of each factor in the two-step procedure for 3-HPA bioconversion. …”
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Papain Covalently Immobilized on Chitosan–Clay Nanocomposite Films: Application in Synthetic and Real White Wine by Ilaria Benucci, Claudio Lombardelli, Ilaria Cacciotti, Marco Esti

“…Finally, the latter biocatalyst (papain on OPT-50 film) was applied for the protein stabilization of two different unfined white wines, and it efficiently reduced both the haze potential and the protein content.…”
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High-Level Production of Catechol from Glucose by Engineered <i>Escherichia coli</i> by Guotian Song, Fengli Wu, Yanfeng Peng, Xiaolong Jiang, Qinhong Wang

“…Protocatechuic acid (PCA) was first efficiently produced by the engineered <i>Escherichia coli</i> strain AAA01 via fermentation, and then PCA in the fermentative broth was converted into CA by the whole-cell biocatalyst AAA12 with PCA decarboxylase. By optimizing the expression of flavin isoprenyl transferases and protocatechuic acid decarboxylases, the titer of CA increased from 3.4 g/L to 15.8 g/L in 12 h through whole-cell biocatalysis, with a 365% improvement; after further optimizing the reaction conditions for whole-cell biocatalysis, the titer of CA achieved 17.7 g/L within 3 h, which is the highest titer reported so far. …”
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GOx/Hb Cascade Oxidized Crosslinking of Silk Fibroin for Tissue-Responsive Wound Repair by Hongdou Shen, Pei Wang, Xiaoke Han, Mengli Ma, Yinghui Shang, Ye Ju, Saiji Shen, Feng Yin, Qigang Wang

“…In this study, a tissue biomolecule-responsive hydrogel matrix, employing natural silk fibroin (SF) as a functional biopolymer and haemoglobin (Hb) as a peroxidase-like biocatalyst, was fabricated through cascade enzymatic crosslinking. …”
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Dynamic Preference for NADP/H Cofactor Binding/Release in <i>E. coli</i> YqhD Oxidoreductase by Rajni Verma, Jonathan M. Ellis, Katie R. Mitchell-Koch

“…Herein, we report the first computational solution-phase structure-dynamics analysis of YqhD, shedding light on the effect of oxidized and reduced NADP/H cofactor binding on the conformational dynamics of the biocatalyst using molecular dynamics (MD) simulations. …”
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Green Synthesis and Molecular Docking Study of Some New Thiazoles Using Terephthalohydrazide Chitosan Hydrogel as Ecofriendly Biopolymeric Catalyst by Jehan Y. Al-Humaidi, Sobhi M. Gomha, Nahed A. Abd El-Ghany, Basant Farag, Magdi E. A. Zaki, Tariq Z. Abolibda, Nadia A. Mohamed

“…Thus, TCs was used as a promising ecofriendly basic biocatalyst for preparation of three new series of thiazoles and two thiadiazoles derivatives via reacting 2-(2-oxo-1,2-diphenylethylidene) hydrazine-1-carbothio-amide with various hydrazonoyl chlorides and α-haloketones under mild ultrasonic irradiation. …”
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Characterization of a Solvent-Tolerant Amidohydrolase Involved in Natural Product Heterocycle Formation by Lea Winand, Dustin Joshua Vollmann, Jacqueline Hentschel, Markus Nett

“…Overall, MxcM is a solvent-stable, halotolerant enzyme with promising biochemical and kinetic properties and, in future, might become a valuable biocatalyst for the manufacturing of pharmaceutical drugs.…”
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Sustainable Biocatalytic Procedure for Obtaining New Branched Acid Esters by María Claudia Montiel, Miguel Asensi, Silvia Gimeno-Martos, Fuensanta Máximo, Josefa Bastida

“…When the synthesis is carried out under the best conditions (70 °C, 10% molar excess of alcohol and six uses of the immobilized enzyme) a productivity of 203.84 kg product × kg biocatalyst⁻¹ is attained. The biocatalytic procedure matches many of the objectives of “green chemistry” and is suitable to be scaled up and used in industrial manufacturing.…”
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Production of Resveratrol Glucosides and Its Cosmetic Activities by Samir Bahadur Thapa, Juhee Jeon, Byung Gyu Park, Dabin Shim, Chang Seok Lee, Jae Kyung Sohng

“…DgAS is recognized as an important biocatalyst due to its high thermostability, effectiveness, and specificity among all known amylosucrases (ASases) in the production of poly-glucosides in a chain of polyphenols, such as resveratrol, making it an ideal candidate for industrial use in the cost-effective production of cosmetic items.…”
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Stability Enhancement of Aldehyde Dehydrogenase from <i>Anoxybacillus geothermalis</i> Strain D9 Immobilized onto Seplite LX120 by Wahhida Latip, Nur Ezzati Rosli, Mohd Shukuri Mohamad Ali, Nor Hafizah Ahmad Kamarudin, Raja Noor Zaliha Raja Abd Rahman

“…Enzyme stability is regarded as an important criterion for an industrial biocatalyst. Aldehyde dehydrogenase (ALDH) from <i>A. geothermalis</i> strain D9 was previously reported to exhibit good thermostability. …”
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Genomic insights into the evolution of flavonoid biosynthesis and O-methyltransferase and glucosyltransferase in Chrysanthemum indicum by Yinai Deng, Peng Yang, Qianle Zhang, Qingwen Wu, Lingfang Feng, Wenjing Shi, Qian Peng, Li Ding, Xukai Tan, Ruoting Zhan, Dongming Ma

“…Next, we construct a one-pot cascade biocatalyst system by combining CiCOMT10, CiUGT11, and our previously identified rhamnosyltransferase, effectively producing diosmin with over 80% conversion from luteolin. …”
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Optimization of Lipase Catalysed Synthesis of Sugar Alcohol Esters Using Taguchi Method and Neural Network Analysis by Adnani, Seyedeh Atena

“…Xylitol esters were successfully prepared from reactions of xylitol with free fatty acids (stearic acid, palmitic acid and capric acid), using enzymes as biocatalyst in an organic solvent system. Such a selective enzymatic route obviates the fastidious regime of substrate activation required in chemical synthesis. …”
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Production of biodiesel from jatropha c. oil using koh and lipase candida r. in an airlift reactor by Liyana, Amer Shah

“…Two-stage transesterification method was used for KOH catalyst reaction while onestage transesterification for biocatalyst reaction. Two-stage transesterfication method was the suitable method used to reduce high free fatty acid content in jatropha oil and increase the biodiesel yield. …”
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Production of recombinant cholesterol oxidase containing covalently bound FAD in <it>Escherichia coli</it> by Molla Gianluca, Pollegioni Loredano, Volontè Federica, Frattini Luca, Marinelli Flavia, Piubelli Luciano

“…It has two major biotechnological applications, i.e. in the determination of serum (and food) cholesterol levels and as biocatalyst providing valuable intermediates for industrial steroid drug production. …”
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Novel Magnetic Polymeric Filters with Laccase-Based Nanoparticles for Improving Congo Red Decolorization in Bioreactors by Diana C. Sotelo, Nancy Ornelas-Soto, Johann F. Osma

“…Results indicate that the novel use of permanent magnetic elements improved the nanoparticle retention in the filters and promoted the mass transfer between the dye and the biocatalyst to enhance wastewater treatment.…”
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Studies on the extremo-lipase produced by the halotolerant Oceanobacillus iheyensis strain QCS by Noura Sh. A. Hagaggi

“…Overall, as the lipase from O. iheyensis strain QCS has a number of interesting properties especially its stability at extreme conditions; it could be used as a potential promising candidate for detergents industry, and as a biocatalyst in low water enzymatic processes.…”
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Enzymatic Production of Biologically Active 3-Methoxycinnamoylated Lysophosphatidylcholine via Regioselctive Lipase-Catalyzed Acidolysis by Marta Okulus, Magdalena Rychlicka, Anna Gliszczyńska

“…The results showed that Novozym 435 is the most effective biocatalyst for this process, while during the examination of organic solvents, heptane was found propriate reaction medium. …”
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PEMANFAATAN BAHAN TUMBUHAN SEBAGAI BIOKATALISATOR DALAM PRODUKSI MINYAK SAWIT KAYA ASAM LEMAK OMEGA-3 ) [Using of Plant Biocatalisator for Omega-3 PUFA -Rich Palm Oil Production] by Jenny Elisabeth1), D. Siahaan1), D. R. H. Simajuntak2)

“…Rice bran and Carica papaya latex (CPL) were used as biocatalyst. Acidolysis between red palm oil and n-3 PUFA concentrate in free fatty acid form was conducted without solvent, with weight ratio of n-3 PUFA concentrate to palm oil being 1:1. …”
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Chemoenzymatic Synthesis of Indole-Containing Acyloin Derivatives by Saad Alrashdi, Federica Casolari, Aziz Alabed, Kwaku Kyeremeh, Hai Deng

“…Our results demonstrate that NzsH displays a considerable substrate profile toward donor substrates for production of acyloins with different indole ring systems, suggesting that NzsH could be further explored as a potential biocatalyst via directed evolution to improve the catalytic efficiency in the future.…”
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