The Stability of Dimeric <i>D</i>-amino Acid Oxidase from Porcine Kidney Strongly Depends on the Buffer Nature and Concentration by Diego Carballares, Javier Rocha-Martin, Roberto Fernandez-Lafuente

“…However, when using an immobilized DAAO biocatalyst which presented enzyme subunits attached to the support, where dissociation was not possible, this effect of the buffer nature on enzyme stability almost disappeared. …”
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Establishment of <i>Kluyveromyces marxianus</i> as a Microbial Cell Factory for Lignocellulosic Processes: Production of High Value Furan Derivatives by Marlene Baptista, Joana T. Cunha, Lucília Domingues

“…Furthermore, one selected isolate was efficiently used as a whole-cell biocatalyst to convert furfural and HMF into their derivatives, furfuryl alcohol and 2,5-bis(hydroxymethyl)furan (BHMF), with high yields and productivities. …”
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Polymer-Based Conductive Nanocomposites for the Development of Bioanodes Using Membrane-Bound Enzyme Systems of Bacteria <i>Gluconobacter oxydans</i> in Biofuel Cells by Veronika Fedina, Daria Lavrova, Tatyana Dyachkova, Anastasia Pasko, Anton Zvonarev, Victor Panfilov, Olga Ponamoreva, Sergey Alferov

“…Chitosan hydrogel in composition with MWCNTox is the most promising material for biocatalyst immobilization for the development of bioelectrochemical systems. …”
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Invertase Immobilization on Magnetite Nanoparticles for Efficient Fructooligosaccharide Generation: A Comprehensive Kinetic Analysis and Reactor Design Strategy by David Polanía Melo, Andrés Hernández Bravo, Juan C. Cruz, Luis H. Reyes

“…In a batch reactor, the reduction or elimination of glucose in the reactive media led to a 2.1% increase in FOS production. Immobilizing the biocatalyst enhanced the overall performance of SInv. …”
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Sustainable mitigation of heavy metals from effluents: Toxicity and fate with recent technological advancements by Vivek Kumar Gaur, Poonam Sharma, Prachi Gaur, Sunita Varjani, Huu Hao Ngo, Wenshan Guo, Preeti Chaturvedi, Reeta Rani Singhania

“…Enzymes secreted by microbes and whole microbial cell are considered an eco-efficient biocatalyst for mitigation of heavy metals from contaminated sites. …”
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Asymmetric Synthesis of Optically Pure Aliphatic Amines with an Engineered Robust ω-Transaminase by Linhan Dong, Qinglong Meng, Carlos Ramírez-Palacios, Hein J. Wijma, Siewert J. Marrink, Dick B. Janssen

“…The results show that <i>Pj</i>TA-R6 is a promising biocatalyst for the asymmetric synthesis of aliphatic amines with a product spectrum that can be explained by its structural features.…”
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Construction of a Novel Chimeric Dextransucrase Fused to the Carbohydrate-Binding Module CBM2a by Reinaldo Fraga Vidal, Roberto Carlos Arísticas Ribalta, Lisandra Teresa Martínez Valdés, Meinardo Lafargue Gámez, Amanda Montes Alvarez, Arianne Rubio Sánchez, Eric Dubreucq, Benoît Moreau

“…This research showed that hybrids and chimeric biocatalyst DSases with novel binding capacity to cellulose can be designed to purify and immobilize using renewable lignocellulosic materials as supports.…”
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Biocatalytic Performance of <i>β</i>-Glucosidase Immobilized on 3D-Printed Single- and Multi-Channel Polylactic Acid Microreactors by Andreas-Georgios Vasios, Anastasia Skonta, Michaela Patila, Haralambos Stamatis

“…The microreactor underwent surface modification with chitosan, and <i>β</i>-glucosidase from <i>Thermotoga maritima</i> was covalently immobilized. The immobilized biocatalyst retained almost 100% of its initial activity after incubation at different temperatures, while it could be effectively reused for up to 10 successful reaction cycles. …”
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Integrating Fermentation Engineering and Organopalladium Chemocatalysis for the Production of Squalene from Biomass-Derived Carbohydrates as the Starting Material by Cuicui Wu, Kaifei Tian, Xuan Guo, Yunming Fang

“…First, glucose derived from hydrolysis of cellulose was used as a renewable resource, using genetically engineered <i>Saccharomyces cerevisiae</i> as the initial biocatalyst to produce β-farnesene with a titer of 27.6 g/L in a 2.5 L bioreactor. …”
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Counterbalance of Stability and Activity Observed for Thermostable Transaminase from <i>Thermobaculum terrenum</i> in the Presence of Organic Solvents by Ekaterina Yu. Bezsudnova, Alena Yu. Nikolaeva, Sergey Y. Kleymenov, Tatiana E. Petrova, Sofia A. Zavialova, Kristina V. Tugaeva, Nikolai N. Sluchanko, Vladimir O. Popov

“…Our data support the idea that the counterbalance of stability and activity is crucial for the catalysis under given conditions; the obtained results may be useful for fine-tuning biocatalyst efficiency.…”
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Biodiesel Production by Lipase-Catalyzed in Situ Transesterification of Rapeseed Oil Containing a High Free Fatty Acid Content with Ethanol in Diesel Fuel Media by Migle Santaraite, Egle Sendzikiene, Violeta Makareviciene, Kiril Kazancev

“…In this study, 11 different lipases were analyzed from which to select the most effective biocatalyst according to the chosen experimental conditions. …”
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A New Stilbene Glucoside from Biotransformation-Guided Purification of Chinese Herb Ha-Soo-Oh by Jiumn-Yih Wu, Hsiou-Yu Ding, Tzi-Yuan Wang, Min-Hui Hsu, Te-Sheng Chang

“…To identify new bioactive compounds in Chinese herbs, the biotransformation-guided purification (BGP) process was applied to Ha-Soo-Oh with <i>Bacillus megaterium</i> tyrosinase (<i>Bm</i>TYR) as a biocatalyst. The result showed that a major biotransformed compound could be purified using the BGP process with preparative high-performance liquid chromatography (HPLC), and it was confirmed as a new compound, 2,3,5,3′,4′-pentahydroxystilbene-2-<i>O</i>-<i>β</i>-glucoside (PSG) following mass and nucleic magnetic resonance (NMR) spectral analyses. …”
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A non-canonical nucleophile unlocks a new mechanistic pathway in a designed enzyme by Amy E. Hutton, Jake Foster, Rebecca Crawshaw, Florence J. Hardy, Linus O. Johannissen, Thomas M. Lister, Emilie F. Gérard, Zachary Birch-Price, Richard Obexer, Sam Hay, Anthony P. Green

“…Interestingly, although MeHis23 to His substitution in BHMeHis1.8 reduces activity by 4-fold, the resulting His containing variant is still a potent MBH biocatalyst. However, analysis of the BHMeHis1.8 evolutionary trajectory reveals that the MeHis nucleophile was crucial in the early stages of engineering to unlock the new mechanistic pathway. …”
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Electricity generation in a ceramic separator microbial fuel cell employing a bacterial consortium for penicillin removal by Pimprapa Chaijak, Alisa Kongthong, Junjira Thipraksa, Panisa Michu

“…Furthermore, when used as a whole-cell biocatalyst in a low-cost upflow MFC, the Morganella morganii-rich SF11 consortium demonstrated the highest voltage and power density of 964.93±1.86 mV and 0.56±0.00 W/m3, respectively. …”
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Antarctic aldehyde dehydrogenase from Flavobacterium PL002 as a potent catalyst for acetaldehyde determination in wine by V. I. Paun, R. M. Banciu, P. Lavin, A. Vasilescu, P. Fanjul-Bolado, C. Purcarea

“…An enzymatic electrochemical assay was developed for acetaldehyde detection in wines with proven accuracy in comparison with the reference spectrophotometric method, thus evidencing the potential of S2-ALDH as effective biocatalyst for industry and biosensing.…”
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Revisiting <i>Jatropha curcas</i> Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model by Marcos Gustavo Araujo Schwarz, Deborah Antunes, Gabriela Coelho Brêda, Richard Hemmi Valente, Denise Maria Guimarães Freire

“…We decided to reevaluate this <i>J. curcas</i> esterase and extend its characterization to check this apparent discrepancy and gain insights into the enzyme’s potential as a biocatalyst. After anion exchange chromatography and two-dimensional gel electrophoresis, we identified the enzyme as belonging to the dienelactone hydrolase family, characterized by a cysteine as the nucleophile in the catalytic triad. …”
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Semi-rational engineering an aldo–keto reductase for stereocomplementary reduction of α-keto amide compounds by Ruixuan Bai, Baoling Chen, Liangyu Zheng

“…In this study, iolS, an aldo–keto reductase from Bacillus subtilis 168 was exhibited as a potential biocatalyst, which could catalyze the reduction of diaryl α-keto amide such as 2-oxo-N, 2-diphenyl-acetamide (ONDPA) with moderate S-selectivity (76.1%, ee) and 60.5% conversion. …”
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Laccase‐Based Self‐Amplifying Catalytic System Enables Efficient Antibiotic Degradation for Sustainable Environmental Remediation by Ying Xia, Liming Xia, Xinda Lin

“…Laccase (LAC) has emerged as a promising biocatalyst for the oxidation of environmentally toxic contaminants with high catalytic efficiency; however, its large‐scale application is hindered by enzyme costs and dependency on redox mediators. …”
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Molecular, structural and biochemical characterization of a novel recombinant chlorophyllase from cyanobacterium Oscillatoria acuminata PCC 6304 by Sitian Gu, Xiaojun Dai, Zhengjun Xu, Qiwen Niu, Jiang Jiang, Yuanfa Liu

“…Conclusions The high-level expression and broad substrate specificity of recombinant OaCLH make it suitable for genetically engineering and a promising biocatalyst for industrial production, with applications in vegetable oil refining and laundry detergents.…”
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Assessing the Thiamine Diphosphate Dependent Pyruvate Dehydrogenase E1 Subunit for Carboligation Reactions with Aliphatic Ketoacids by Stefan R. Marsden, Duncan G. G. McMillan, Ulf Hanefeld

“…Here, we show that <i>Ec</i>PDH E1 is a promising biocatalyst for the production of chiral α-hydroxyketones. …”
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