Thermophiles and carbohydrate-active enzymes (CAZymes) in biofilm microbial consortia that decompose lignocellulosic plant litters at high temperatures by Liew, Kok Jun, Liang, Chee Hung, Lau, Yee Ting, Yaakop, Amira Suriaty, Chan, Kok Gan, Shahar, Saleha, Shamsir, Mohd. Shahir, Goh, Kian Mau

“…With the advancement of next-generation sequencing, it is now possible to mine many new biocatalyst sequences. In this study, we aimed to (i) identify the metataxonomic of prokaryotes and eukaryotes in microbial mats using 16S and 18S rRNA markers, (ii) and explore carbohydrate degrading enzymes (CAZymes) that have a high potential for future applications. …”
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In-situ fabrication of functionalized starch magnetic nanoparticles for immobilization of laccase by Suhaimi, Suhaily, Jaafar, Nardiah Rizwana, Jailani, Nashriq, A. Rahman, Roshanida, Ngadi, Norzita, Abdul Murad, Abdul Munir, Hashim, Noor Haza Fazlin, Md. Illias, Rosli

“…Therefore, yield a promising biocatalyst to be applied in various fields.…”
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Statistical modelling of eugenol benzoate synthesis using rhizomucor miehei lipase reinforced nanobioconjugates by Abd. Manan, Fatin Myra, Abd. Rahman, Ida Nurhazwani, Che Marzuki, Nur Haziqah, Mahat, Naji Arafat, Huyop, Fahrul, Abdul Wahab, Roswanira

“…Therefore, the RML/CS/MWCNTs developed here appear to be a promising alternative yet environmentally friendly biocatalyst for a sustainable production of eugenol benzoate.…”

Bioconversion of starch to maltooligosaccharides (MOS) by the reaction of maltogenic amylase by Ahmad, Rabi’atul Adawiyah, Jaafar, Nardiah Rizwana, Abdul Manas, Nor Hasmaliana, Wan Azelee, Nur Izyan, Md. Illias, Rosli, Nawawi, Noor Namirah

“…Its ability to utilise multiple substrates and catalyse hydrolysis and transglycosylation reactions simultaneously makes it a unique biocatalyst. The catalysis could be exploited in many ways to obtain oligosaccharides of different lengths and various modified sugars. …”
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Enhancement of bioelectricity production from soil microbial fuel cell (SMFC) by additional glucose, nutrient broth and escherichia coli bacteria by Raiyan Saleh, Shahirah, Abd. Jalil, Nur Kamilah, Asli, Umi Aisah, Khamis, Aidee Kamal, Johari, Anwar, Nor Majid, Nadzreeq, Sasongko, Nugroho A., Ramadhan, Wisnu

“…Soil Microbial Fuel Cell (SMFC) is a device that using bacteria in soils as a biocatalyst. These bacteria, called exoelectrogenic bacteria are oxidizing organic substrates to release electrons, which then harvested in an external circuit to produce bioelectricity. …”

Screening of culture conditions for production of xylanase from landfill soil bacteria by Siti Nor Amira, Rosli, Rohaida, Che Man, N., Masngut

“…Enzymatic method can be used for the degradation of these materials involving the use of microbial enzymes [3] that are less polluting, environmental friendly, energy saving and lower disposal problems [4]. Xylanase is a biocatalyst which specifically degrade xylan into smaller sugars such as xylose and xylobiose [5]. …”
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Rational Design of Disulfide Bonds Increases Thermostability of a Mesophilic 1,3-1,4-β-Glucanase from Bacillus terquilensis. by Chengtuo Niu, Linjiang Zhu, Xin Xu, Qi Li

“…1,3-1,4-β-glucanase is an important biocatalyst in brewing industry and animal feed industry, while its low thermostability often reduces its application performance. …”
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Transglycosylation Activity of Engineered <i>Bifidobacterium</i> Lacto-<i>N</i>-Biosidase Mutants at Donor Subsites for Lacto-<i>N</i>-Tetraose Synthesis by Mireia Castejón-Vilatersana, Magda Faijes, Antoni Planas

“…Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield. It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.…”
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The Isoenzymic Diketocamphane Monooxygenases of <i>Pseudomonas putida</i> ATCC 17453—An Episodic History and Still Mysterious after 60 Years by Andrew Willetts

“…First referred to as ‘ketolactonase—an enzyme for cyclic lactonization’—the enzyme now classified as 2,5-diketocamphane 1,2-monooxygenase (EC 1.14.14.108) holds a special place in the history of oxygen-dependent biochemistry, being the first biocatalyst confirmed to undertake a biooxygenation reaction equivalent to the peracid-catalysed Baeyer–Villiger chemical oxidation first reported in the late 19th century. …”
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Improved Solubility and Stability of a Thermostable Carbonic Anhydrase via Fusion with Marine-Derived Intrinsically Disordered Solubility Enhancers by Byung Hoon Jo

“…Carbonic anhydrase (CA), an enzyme catalyzing the reversible hydration reaction of carbon dioxide (CO₂), is considered a promising biocatalyst for CO₂ reduction. The α-CA of <i>Thermovibrio ammonificans</i> (taCA) has emerged as a compelling candidate due to its high thermostability, a critical factor for industrial applications. …”
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Arabinogalactan-Proteins as Boron-Acting Enzymes, Cross-Linking the Rhamnogalacturonan-II Domains of Pectin by Rifat Ara Begum, Stephen C. Fry

“…However, the sub-cellular locations, pH dependence, reversibility and biocatalyst involvement in borate bridging remain uncertain. …”
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Characterization of a New L-Glutaminase Produced by <i>Achromobacter xylosoxidans</i> RSHG1, Isolated from an Expired Hydrolyzed L-Glutamine Sample by Rabia Saleem, Safia Ahmed

“…As significant biocatalyst, L-glutaminases find potential applications in various fields, from nourishment to the pharmaceutical industry. …”
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Simultaneous Extraction of Rapeseed Oil and Enzymatic Transesterification with Butanol in the Mineral Diesel Medium by Egle Sendzikiene, Violeta Makareviciene, Migle Santaraite

“…The investigation of the in situ process was performed using a mixture of butanol and mineral diesel and the most effective biocatalyst <i>Lipozyme TL IM</i> was selected. The novelty of this paper consists of the fact that mineral diesel was used as the oil extractant, and the amount chosen was such that, at the end, a mixture of fuel with a ratio 9:1 of mineral diesel to biodiesel was be produced. …”
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Stabilization of b-Glucuronidase by Immobilization in Magnetic-Silica Hybrid Supports by Sonali Correa, Magdalena Ripoll, Erienne Jackson, Valeria Grazú, Lorena Betancor

“…Integration of MNPs provided the advantage of reusing the biocatalyst via magnetic separation up to six times with residual activity. …”
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A Pseudomonas putida efflux pump acts on short-chain alcohols by Georg Basler, Mitchell Thompson, Danielle Tullman-Ercek, Jay Keasling

“…Together with the increased interest in P. putida for metabolic engineering due to its flexible metabolism, high native tolerance to toxic substances, and various applications of engineering its metabolism, our findings endorse the strain as an excellent biocatalyst for the high-yield production of next-generation biofuels.…”
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Comparative Evaluation of the Asymmetric Synthesis of (S)-Norlaudanosoline in a Two-Step Biocatalytic Reaction with Whole <i>Escherichia coli</i> Cells in Batch and Continuous Flow... by Adson Hagen Arnold, Kathrin Castiglione

“…The continuous production at two scales (5 mL and 50 mL columns) revealed insufficient system stability originating from biocatalyst leaching and inactivation. Thus, while the use of whole cells in batch biotransformations represents an immediate process improvement, the transfer to flow catalysis needs further optimization.…”
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Repurposing Glutathione Transferases: Directed Evolution Combined with Chemical Modification for the Creation of a Semisynthetic Enzyme with High Hydroperoxidase Activity by Irene Axarli, Farid Ataya, Nikolaos E. Labrou

“…Such an engineered GPX-like biocatalyst based on the GSTU scaffold might serve as a catalytic bioscavenger for the detoxification of hazardous hydroperoxides. …”
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Graphite–Metal Oxide Composites as Potential Anodic Catalysts for Microbial Fuel Cells by Elitsa Chorbadzhiyska, Ivo Bardarov, Yolina Hubenova, Mario Mitov

“…Ag/AgCl) in the presence of pure culture <i>Pseudomonas putida</i> 1046 used as a model biocatalyst. The obtained data showed that the applied poising resulted in a generation of anodic currents, which gradually increased during the long-term experiments, indicating a formation of electroactive biofilms on the electrode surfaces. …”
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Diversifying Arena of Drug Synthesis: In the Realm of Lipase Mediated Waves of Biocatalysis by Sahil Verma, Rahul Narayanlal Choudhary, Akash Prakash Kanadje, Uttam Chand Banerjee

“…An upcoming wave demands fulfilling the vision of tailored lipase whilst a far-flung exploration needs to be unveiled for various research impediments in rendering lipase as a custom fit biocatalyst in pharmaceutical industry.…”
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Entrapment of the Fastest Known Carbonic Anhydrase with Biomimetic Silica and Its Application for CO₂ Sequestration by Chia-Jung Hsieh, Ju-Chuan Cheng, Chia-Jung Hu, Chi-Yang Yu

“…R5-SazCA-SP shows great potential as a robust and efficient biocatalyst for CO₂ sequestration because of its high activity, high stability, and reusability.…”
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