Cross-linked enzyme aggregates of recombinant xylanase for conversion of hemicelluloses to xylooligosaccharides by Md. Shaarani @ Md. Nawi, Shalyda

“…Kinetic parameters confirmed that CLEA immobilization did affect the productivity of the designed biocatalyst.…”
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entrapment of porous cross-linked enzyme aggregates of maltogenic amylase from bacillus lehensis g1 into calcium alginate for maltooligosaccharides synthesis by Nawawi, N. N., Hashim, Z., Rahman, R. A., Murad, A. M.A., Bakar, F. D. A., Illias, R. M.

“…Finally, the reusability of Mag1-p-CLEAs-CA makes them as a potential biocatalyst for the continuous synthesis of MOSs.…”

Catalyst Replacement Policy on Multienzymatic Systems: Theoretical Study in the One-Pot Sequential Batch Production of Lactofructose Syrup by Pablo Silva, Vanessa Arancibia, Daniela Cid, Oscar Romero, Andrés Illanes, Lorena Wilson

“…This work shows the feasibility of conducting a one-pot operation with immobilized enzymes of quite different operational stability, and that a proper strategy of biocatalyst replacement increases the productivity of the process.…”
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Characterization of a Novel Alginate Lyase from Marine Bacterium Vibrio furnissii H1 by Xiaoyan Zhu, Xiangqian Li, Hao Shi, Jia Zhou, Zhongbiao Tan, Mengdi Yuan, Peng Yao, Xiaoyan Liu

“…Alginate lyases show great potential for industrial and medicinal applications, especially as an attractive biocatalyst for the production of oligosaccharides with special bioactivities. …”
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Structure–Function Relationship Study of a Secretory Amoebic Phosphatase: A Computational-Experimental Approach by Celina Terán-Ramírez, Rosa E. Mares-Alejandre, Ana L. Estrada-González, Patricia L. A. Muñoz-Muñoz, Marco A. Ramos-Ibarra

“…To assess whether it has the potential as a biocatalyst in removing phosphate groups from natural substrates, we studied the <i>Eh</i>HAPp49 structural and functional features using a computational-experimental approach. …”
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Design of a New Gemini Lipoaminoacid with Immobilized Lipases Based on an Eco-Friendly Biosynthetic Process by Patrícia M. Carvalho, Rita C. Guedes, Maria R. Bronze, Célia M. C. Faustino, Maria H. L. Ribeiro

“…Moreover, a new medium was tested where dodecylamine was melted and added to the cystine and to the biocatalyst, building a system of mainly undissolved substrates, leading to 5 mg/mL of LAA. …”
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Optimizing the Production of Recombinant Hydroperoxide Lyase in <i>Escherichia coli</i> Using Statistical Design by Sophie Vincenti, Magali Mariani, Jessica Croce, Eva Faillace, Virginie Brunini-Bronzini de Caraffa, Liliane Berti, Jacques Maury

“…To meet the growing demand for natural GLVs, the use of recombinant HPL as a biocatalyst in enzyme-catalyzed processes appears to be an interesting application. …”
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Efficient Biosynthesis of (<i>S</i>)-1-chloro-2-heptanol Catalyzed by a Newly Isolated Fungi <i>Curvularia hominis</i> B-36 by Shenpeng Xu, Qinzhe Lin, Wentian Chen, Ruoyu Lin, Yikai Shen, Pinchuan Tang, Sisi Yu, Wenting Du, Jun Li

“…The reductive capacity of <i>Curvularia hominis</i> B-36 was investigated as a whole-cell biocatalyst in the bioreduction, and the excellent yield (97.2%) and enantiomeric excess (ee) value (99.9%) were achieved under the optimal conditions as follows: 75 mM 1-chloro-2-heptanone, K₂HPO₄-KH₂PO₄ (100 mM, pH 6.0), 50 g L⁻¹ resting cells (dry cell weight; DCW), 15% (<i>v</i>/<i>v</i>) isopropanol as co-substrate, 200 rpm, 30 °C, 20 h. …”
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Computational-Designed Enzyme for β-Tyrosine Production in Lignin Valorization by Fei Peng, Habibu Aliyu, André Delavault, Ulrike Engel, Jens Rudat

“…In summary, we report a β-tyrosine synthesis process from a monolignol component, offering a new way for lignin valorization by biocatalyst modification.…”
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Cross- linked enzyme aggregates of recombinantxylanase for hemicelluloses to xylooligosaccharides by Shalyda, Md Shaarani

“…Kinetic parameters confirmed that CLEA immobilization did affect the productivity of the designed biocatalyst.…”
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The 8-bromobaicalein inhibited the replication of dengue, and Zika viruses and targeted the dengue polymerase by Siwaporn Boonyasuppayakorn, Thanaphon Saelee, Thao Nguyen Thanh Huynh, Rita Hairani, Kowit Hengphasatporn, Naphat Loeanurit, Van Cao, Vipanee Vibulakhaophan, Panattida Siripitakpong, Parveen Kaur, Justin Jang Hann Chu, Chairat Tunghirun, Opas Choksupmanee, Sarin Chimnaronk, Yasuteru Shigeta, Thanyada Rungrotmongkol, Warinthorn Chavasiri

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Coupling of Bioreaction and Separation via Novel Thermosensitive Ionic Liquids Applied in the Baker’s Yeast-Catalyzed Reduction of Ethyl 2-oxo-4-phenylbutyrate by Yuexi Yang, Yugang Shi, Lifang Feng, Shiyi Tian

“…The use of baker’s yeast to reduce ethyl 2-oxo-4-phenylbutyrate (EOPB) in conventional biphasic systems is hindered by low productivities due to mass transfer resistance between the biocatalyst and the substrate partitioned into two different phases. …”
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Enzymatic Preparation of Gentiooligosaccharides by a Thermophilic and Thermostable β-Glucosidase at a High Substrate Concentration by Wei Xia, Lingling Sheng, Wanmeng Mu, Yuping Shi, Jing Wu

“…KOL6 was used as a biocatalyst for the synthesis of GnOS. TsBgl1 exhibited excellent thermophilic and thermostable properties by possessing a melting temperature of 101.5 °C and reacting at 80–90 °C efficiently. …”
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Characterization and Preliminary Application of a Novel Lipoxygenase from <i>Enterovibrio norvegicus</i> by Bingjie Zhang, Meirong Chen, Bingjie Xia, Zhaoxin Lu, Kuan Shiong Khoo, Pau Loke Show, Fengxia Lu

“…Lipoxygenases have proven to be a potential biocatalyst for various industrial applications. However, low catalytic activity, low thermostability, and narrow range of pH stability largely limit its application. …”
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Joint Multi-Optimization of an Extremophilic Microbial Bioanode for Mitigation of Mixed Hazardous Azo Dyes in Textile Synthetic Wastewater by Sirine Saadaoui, Benjamin Erable, Luc Etchevery, Ameur Cherif, Habib Chouchane

“…All experiments were conducted in electrochemical reactors of final volume 750 mL inoculated with 80% of enrichment medium containing three azo dyes at a concentration of 300 ppm and 20% of biocatalyst microbial SCD source, at 45 °C. The optimal levels were predicted using NemrodW software as carbon felt (CF) anode material, 6 cm² anode surface, 7 g/L glucose concentration, and −0.1 V applied potential. …”
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Optimized enantioselective (S)-2-hydroxypropiophenone synthesis by free- and encapsulated-resting cells of Pseudomonas putida by Reihaneh Kordesedehi, Mohammad Ali Asadollahi, Azar Shahpiri, Davoud Biria, Pablo Iván Nikel

“…BFD produced by induced P. putida resting cells is a highly active biocatalyst without any further treatment in comparison with partially purified enzyme preparations. …”
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Assisting Multitargeted Ligand Affinity Prediction of Receptor Tyrosine Kinases Associated Nonsmall Cell Lung Cancer Treatment with Multitasking Principal Neighborhood Aggregation by Fahsai Nakarin, Kajjana Boonpalit, Jiramet Kinchagawat, Patcharapol Wachiraphan, Thanyada Rungrotmongkol, Sarana Nutanong

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Production, Biochemical Characterization, and Kinetic/Thermodynamic Study of Inulinase from <i>Aspergillus terreus</i> URM4658 by Rodrigo Lira de Oliveira, Suzana Pedroza da Silva, Attilio Converti, Tatiana Souza Porto

“…These results indicate that <i>A. terreus</i> URM4658 inulinase is a promising and efficient biocatalyst, which could be fruitfully exploited in long-term industrial applications.…”
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Enzymatic Synthesis and Molecular Modelling Studies of Rhamnose Esters Using Lipase from <i>Pseudomonas stutzeri</i> by Cecilia García-Oliva, Almudena Perona, Ángel Rumbero, Pilar Hoyos, María J. Hernáiz

“…Herein, we describe for the first time the enzymatic synthesis of rhamnose fatty acid esters by the transesterification of rhamnose with fatty acid vinyl esters, using lipase from <i>Pseudomonas stutzeri</i> as a biocatalyst. The use of this lipase allows excellent catalytic activity in the synthesis of 4-<i>O</i>-acylrhamnose (99% conversion and full regioselectivity) after 3 h of reaction using tetrahydrofuran (THF) as the reaction media and an excess of vinyl laurate as the acyl donor. …”
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New Cardenolides from Biotransformation of Gitoxigenin by the Endophytic Fungus <i>Alternaria eureka</i> 1E1BL1: Characterization and Cytotoxic Activities by Erdal Bedir, Çiğdem Karakoyun, Gamze Doğan, Gülten Kuru, Melis Küçüksolak, Hasan Yusufoğlu

“…Our results suggest that <i>A. eureka</i> is an effective biocatalyst for modifying cardenolide-type secondary metabolites.…”
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