Optimized protocol for cryopreservation of human eggs improves developmental competence and implantation of resulting embryos by Wang Cassie T, Liang Lifeng, Witz Craig, Williams Dan, Griffith Jason, Skorupski Josh, Haddad Ghassan, Gill Jimmy, Wang Weihua

Subjects: “…Blastocyst…”
Get full text

Pregnancy outcomes in patients with polycystic ovary syndrome who conceived after single thawed blastocyst transfer: a propensity score-matched study by Hui-Ying Jie, Xiu Zhou, Ming-Peng Zhao, Min Hu, Qing-Yun Mai, Can-Quan Zhou

Subjects: Get full text

Comparison of aneuploidy rate in spontaneous abortion chorionic villus between D6 and D5 thawed-frozen blastocyst transfer by Weie Zhao, Panyu Chen, Xiaoping Liu, Yujie Li, Xiaoyan Liang, Jingjie Li

Subjects: “…Blastocyst development speed…”
Get full text

Clinical outcomes of personalized blastocyst embryo transfer after endometrial receptivity analysis: A multicenter, retrospective cohort study by Yuya Takeshige, Seung Chik Jwa, Yasushi Hirota, Yutaka Osuga, Takeshi Kuramoto, Yasuyuki Mio, Kenji Furui, Masayuki Kinutani, Masahide Shiotani, Yoshimasa Asada, Hirobumi Kamiya, Hiroaki Yoshida, Hideki Igarashi, Koichi Kyono

“…Methods This was a multicenter, retrospective cohort study involving 861 women who underwent ERA testing at certified fertility clinics in Japan, and who received subsequent personalized blastocyst embryo transfers (ET) between 2018 and 2020. …”
Get full text

Genome-wide assessment of DNA methylation alterations induced by superovulation, sexual immaturity and in vitro follicle growth in mouse blastocysts by Laura Saucedo-Cuevas, Elena Ivanova, Anamaria-Cristina Herta, Felix Krueger, Katy Billooye, Johan Smitz, Gavin Kelsey, Ellen Anckaert

“…Here, we provide genome-wide DNA methylation profiles of blastocysts generated after superovulation of prepubertal or adult mice, compared with blastocysts derived from non-stimulated adult mice. …”
Get full text

Biphasic oxygen tension promotes the formation of transferable blastocysts in patients without euploid embryos in previous monophasic oxygen cycles by Hsiu-Hui Chen, Chun-I Lee, Chun-Chia Huang, En-Hui Cheng, Tsung-Hsien Lee, Pin Yao Lin, Chien-Hong Chen, Maw-Sheng Lee

“…Abstract This study evaluated whether the concentration of biphasic O2 (5–2%) promotes the formation of qualified blastocysts (QBs) and euploid blastocysts and the probability of cycles with transferable blastocysts. …”
Get full text

#105 : Single Day 5 Versus Day 6 Euploid Blastocyst Transfer: The Impact of Embryo Quality on Pregnancy Outcomes by Thang Le, Thuy Nguyen, Quy Phan, Anh Phi, Phuong Giap, Huong Nguyen, Hoang Le

Subjects: Get full text

Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst by Dae-Jin Kwon, Kyung-Do Park, Lee HakKyo, Jae-Don Oh

“…This study investigated the blastocyst productivity of in vitro culture (IVC) medium (synthetic oviductal fluid medium with amino acids, mSOFaa) supplemented with 5% fetal bovine serum (FBS, Control) or LR-BSA (1, 2.5, 5 mg/ml dosages, respectively) and the qualitative characteristics of produced blastocysts. …”
Get full text

Protease secretions by the invading blastocyst induce calcium oscillations in endometrial epithelial cells via the protease-activated receptor 2 by Aurélie Hennes, Johanna Devroe, Katrien De Clercq, Martina Ciprietti, Katharina Held, Katrien Luyten, Nele Van Ranst, Nina Maenhoudt, Karen Peeraer, Hugo Vankelecom, Thomas Voets, Joris Vriens

“…Proteases have been described as blastocyst-secreted proteins involved in the hatching process and early implantation events. …”
Get full text

Diamour®, a newly vitrification device for human blastocysts, provides the same efficient perinatal outcomes as the commonly used Cryotop® by Masashi Yoshida, Shiori Abe, Yumi Koyanagi, Marina Nakano, Takao Miyake

“…The Diamour®-vitrified blastocysts yielded 30 live births, and the comparator-vitrified blastocysts 29. …”
Get full text

Correlation between controlled ovarian stimulation protocols and euploid blastocyst rate in pre-implantation genetic testing for aneuploidy cycles by Bixia Huang, Hui Li, Bin Xu, Ning Li, Xiaofei Wang, Yanping Li, Jing Zhao

“…Abstract Background Several studies have explored which COS protocol yields a higher blastocyst euploidy rate, but findings have been inconsistent. …”
Get full text

A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture medium by Somayeh Barzanouni, Farideh Moramezi, Mahvash Zargar, Hamid Galehdari, Masoud Hemadi

Subjects: “…preimplantation diagnosis, embryo implantation, culture media, blastocyst, polymerase chain reaction.…”
Get full text

Developmental potency of human ES cell-derived mesenchymal stem cells revealed in mouse embryos following blastocyst injection by Borong Huang, Siyi Fu, Yanan Hao, Cheung Kwan Yeung, Xin Zhang, Enqin Li, Xiaoling Xu, Ningyi Shao, Ren-He Xu

“…Thus, this study provides evidence for the stemness and developmental capability of human MSCs through chimerization with the mouse blastocyst, serving as a model for studying human mesenchymal and skeletal development.…”
Get full text

Cleavage-stage or blastocyst-stage embryo biopsy has no impact on growth and health in children up to 2 years of age by Florence Belva, Fiskani Kondowe, Anick De Vos, Kathelijn Keymolen, Andrea Buysse, Frederik Hes, Veerle Berckmoes, Pieter Verdyck, Willem Verpoest, Martine De Rycke

Subjects: Get full text

In Vitro-Produced Equine Blastocysts Exhibit Greater Dispersal and Intermingling of Inner Cell Mass Cells than In Vivo Embryos by Muhammad Umair, Veronica Flores da Cunha Scheeren, Mabel M. Beitsma, Silvia Colleoni, Cesare Galli, Giovanna Lazzari, Marta de Ruijter-Villani, Tom A. E. Stout, Anthony Claes

Subjects: Get full text

#360 : Twin Pregnancy After Step-Wise Cryopreservation and Thawing of Oocytes, Sperm and Blastocysts in the Case of Azoospermia: Case Report by Hee Jung Kang, Seul Ki Song, Su Vin Jo, You Jung Lee, Gyoung Hoon Lee

“…Among five cryopreserved embryos, three were at the cleavage, and two were at the blastocyst stage. Two blastocyst embryos were thawed on the sixth day of progesterone treatment after pretreatment with estradiol valerate. …”
Get full text

A proof of concept for a deep learning system that can aid embryologists in predicting blastocyst survival after thaw by P. Marsh, D. Radif, P. Rajpurkar, Z. Wang, E. Hariton, S. Ribeiro, R. Simbulan, A. Kaing, W. Lin, A. Rajah, F. Rabara, M. Lungren, U. Demirci, A. Ng, M. Rosen

“…The objective of this study was to develop a proof of concept deep learning model capable of assisting embryologist assessment of survival of thawed blastocysts prior to embryo transfer. A deep learning model was developed using 652 labeled time-lapse videos of freeze–thaw blastocysts. …”
Get full text

A retrospective comparative study of double cleavage-stage embryo transfer versus single blastocyst in frozen-thawed cycles by Yun-Hsuen Lim, Poo Keen Sim, Prashant Nadkarni

“…Abstract Background This retrospective study aimed to compare the outcomes of day 3 double embryo transfer (DET) with single blastocyst transfer (SBT) during frozen embryo transfer (FET) cycles. …”
Get full text

Competent blastocyst and receptivity endometrium improved clinical pregnancy in fresh embryo transfer cycles: a retrospective cohort study by Longmei Wang, Pingping Qiu, Lizhi Jiang, Ping Li, Yufei Jiang

Subjects: “…Available blastocyst formation rate…”
Get full text

Transcriptomic signatures in trophectoderm and inner cell mass of human blastocysts classified according to developmental potential, maternal age and morphology. by Yoshiteru Kai, Hailiang Mei, Hiroomi Kawano, Naotsuna Nakajima, Aya Takai, Mami Kumon, Azusa Inoue, Naoki Yamashita

“…Here, we performed RNA-Seq analysis of TE and ICM of human blastocysts, the pregnancy expectation of which was retrospectively determined using the clinical outcomes of 1,890 cases of frozen-thawed blastocyst transfer. …”
Get full text