#103 : The Clinical Outcomes of Lasered Assisted Hatching on Single Frozen-Thawed Blastocyst Cycle: A Retrospective, Observational Study by May Kee Chong, Sue Hwee Ng, Poo Keen Sim

“…Hence, the aim of this study was to evaluate the effect of LAH toward clinical outcomes including clinical pregnancy, miscarriage, and ectopic pregnancy rate on single frozen-thawed blastocyst cycle. Method: A total of 1553 single frozen-thawed blastocyst cycles (LAH, n=116; non-LAH, n=1417) with patient’s mean age of 35 ± 0.06 were recruited from 2018 to 2022. …”
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Ovarian endometrioma increases the embryo aneuploid rate: an analysis of 7092 biopsied blastocysts from fertile monogenetic disease carriers by Niwei Yan, Xi Yuan, Sunxing Huang, Huiying Jie, Jing Wang, Yuan Yuan

“…The euploid rate of the study group was significantly lower than that of the control group (52.6% vs. 61.8%, p = 0.012), while the oocyte maturation, fertilization, usable embryo and blastocyst formation rates were comparable. Adjusted for basal FSH level, starting stimulating gonadotropin dosage, total gonadotropin dosage and FSH level on hCG day, euploid rate was still negatively related to endometrioma status. …”
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Effects of murine strain, blastocyst stage and inner cell mass isolation technique on the efficacy of murine embryonic stem cells by Abdullah, Ramli, Wan Khadijah, W.E., Goh, S.Y.

“…The aim of this study was to compare the effects of murine strain, blastocyst stage and inner cell mass (ICM) isolation technique on the efficiency of deriving murine embryonic stem cell (mESC) lines. …”

Analyzing the dynamic measurement of growth and development in children born after the transplantation of poor quality blastocyst embryos: a propensity matching study by Chunmei Yu, Lijing Bai, Wanchao Zhang, Lin Feng, Xiaoyu Wang, Li Chen, Lingmin Hu

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Vitrification of porcine immature oocytes and zygotes results in different levels of DNA damage which reflects developmental competence to the blastocyst stage. by Tamás Somfai, Seiki Haraguchi, Thanh Quang Dang-Nguyen, Hiroyuki Kaneko, Kazuhiro Kikuchi

“…However, total cell numbers and percentages of apoptotic cells (measured by TUNEL) in resultant blastocysts were not different from those of the non-vitrified control. …”
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Ruthenium red improves blastocyst developmental competence by regulating mitochondrial Ca2+ and mitochondrial functions in fertilized porcine oocytes in vitro by Ho-Geun JEGAL, Hyo-Jin PARK, Jin-Woo KIM, Seul-Gi YANG, Min-Ji KIM, Deog-Bon KOO

“…However, the effect of RR on mitochondria-Ca2+ (mito-Ca2+) levels during in vitro fertilization (IVF) on subsequent blastocyst developmental capacity in porcine is unclear. …”
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Birth of monozygotic dichorionic twins after a single blastocyst embryo transfer: a case report of genetic determination of zygosity by Neli Semrl, M.D., Michael Barth, M.D., Sarah Feigl, M.D., Rüdiger Hochstätter, M.D., Irmgard Oreskovic, M.Sc., Herbert Fluhr, M.D., M.H.B.A., Philipp Klaritsch, M.D., Irene Speicher, M.D., Martina Kollmann, M.D., Ph.D.

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A novel machine-learning framework based on early embryo morphokinetics identifies a feature signature associated with blastocyst development by S. Canosa, N. Licheri, L. Bergandi, G. Gennarelli, C. Paschero, M. Beccuti, D. Cimadomo, G. Coticchio, L. Rienzi, C. Benedetto, F. Cordero, A. Revelli

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Bovine conceptus of Bos indicus produced by somatic cell nuclear transfer and parthenogenesis present morphological variations since the blastocyst stage by F.D. Oliveira, J.R. Sangalli, F.V. Meirelles, F. Perecin, P.P.C. Silva Filho, Y.F. Watanabe, M.A. Miglino, A.C. Assis Neto

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Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts by Sha Yang, Yuze Yang, Haisheng Hao, Weihua Du, Yunwei Pang, Shanjiang Zhao, Huiying Zou, Huabin Zhu, Peipei Zhang, Xueming Zhao

“…Furthermore, the blastocyst rate, and blastocyst total cell number and survival rate after vitrification of the EGF+IGF-1+Cx37 group, were significantly higher than those of the control group (<i>p</i> < 0.05), but lower than those of the FSH+LH+EGF+IGF-1+Cx37 group (<i>p</i> < 0.05). …”
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Natural cycle increases the live-birth rate compared with hormone replacement treatment for frozen-thawed single euploid blastocyst transfer by Xiaofang Li, Yan’e Gao, Juanzi Shi, Wenhao Shi, Haiyan Bai

“…In order to mitigate the potential effect of embryos per se, several researchers have worked on this subject for euploid blastocyst transfer, but the results were still inconsistent. …”
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The presence of partial compaction patterns is associated with lower rates of blastocyst formation, sub-optimal morphokinetic parameters and poorer morphologic grade by Christine Hur, Vaani Nanavaty, Meng Yao, Nina Desai

“…Abstract Background Compaction is an important marker of embryonic genome activation and marks a critical step in the development to blastocyst. The objective of our study was to determine whether visualization of the embryonic compaction process through time-lapse imaging (TL) can assist in predicting the kinetics of embryo development as well as the likelihood for blastocyst formation, grade, or ploidy. …”
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Conceptus-endometrial interactions and reproductive hormone profiles during embryonic diapause and reactivation of the blastocyst in the European roe deer (Capreolus capreolus) by Roger T. Lambert

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Influencing factors and predictive model of live birth involving low-grade blastocyst frozen–thawed transfer: a retrospective study by Yuan Fang, Ye He, Wanlu Wang, Zhiguo Zhang, Ping Zhou, Yunxia Cao, Xiaojin He, Yuping Xu, Zhaolian Wei

Subjects: “…Low-grade blastocyst…”
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Day-3-embryo fragmentation is associated with singleton birth weight following fresh single blastocyst transfer: A retrospective study by Jiali Cai, Jiali Cai, Lanlan Liu, Lanlan Liu, Jinghua Chen, Zhenfang Liu, Xiaoming Jiang, Haixiao Chen, Jianzhi Ren

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A validated model for individualized prediction of pregnancy outcome in woman after fresh cycle of Day 5 single blastocyst transfer by Lei Chen, Ruyu Jiang, Yiqun Jiang, Yuting Su, Shanshan Wang

“…Abstract The association between the embryo quality, clinical characteristics, miRNAs (secreted by blastocysts in the culture medium) and pregnancy outcomes has been well-established. …”
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Uterine WNTS modulates fibronectin binding activity required for blastocyst attachment through the WNT/CA2+ signaling pathway in mice by Yuefei Lou, Laurie Pinel, Daniel Dufort

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Scd1 Deficiency in Early Embryos Affects Blastocyst ICM Formation through RPs-Mdm2-p53 Pathway by Huimin Niu, Anmin Lei, Huibin Tian, Weiwei Yao, Ying Liu, Cong Li, Xuetong An, Xiaoying Chen, Zhifei Zhang, Jiao Wu, Min Yang, Jiangtao Huang, Fei Cheng, Jianqing Zhao, Jinlian Hua, Shimin Liu, Jun Luo

“…Analysis of transcripts at key stages of embryo development revealed that Scd1 was important and expressed at an increased level during the cleavage and blastocyst stages. Knockout <i>Scd1</i> gene by CRISPR/Cas9 from zygotes revealed a decrease in lipid droplets (LDs) and damage in the inner cell mass (ICM) formation of blastocyst. …”
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Laser-assisted hatching in lower grade cleavage stage embryos improves blastocyst formation: results from a retrospective study by Weihai Xu, Ling Zhang, Lin Zhang, Zhen Jin, Limei Wu, Shishi Li, Jing Shu

Subjects: “…Blastocyst…”
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Implementation of Noninvasive Preimplantation Genetic Testing for Aneuploidy with Spent Blastocyst Medium into Clinical and Laboratory Practice: The First Successful Experience in Taiwan by Chun-Kai Chen, Wei-Han Huang, Tiencheng Arthur Chang, Kuang-Han Chao, Wang-Fung Chiang, Hsin-Yi Yeh, Szu-Yu Shen

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