Short-interval second ejaculation improves sperm quality, blastocyst formation in oligoasthenozoospermic males in ICSI cycles: a time-lapse sibling oocytes study by Yaoxuan Li, Shikai Wang, Dawen Li, Yueyue Huang, Haifang Liu, Xiaohui Zhang, Jie Qin, Xianbao Mao, Zhengda Li, Liangshi Chen, Pingpin Wei, Wen Shi, Lintao Xue

“…Semen parameters, embryo development efficiency, morphokinetic parameters and clinical outcomes were compared between the two groups to assess the efficiency and clinical value of short-interval second ejaculation in ICSI cycles.ResultsShort-interval second ejaculation significantly improved sperm motility, normal morphological rate, and sperm DNA integrity both before and after sperm swim-up. The high-quality blastocyst rate (24.79% versus 14.67%), available blastocyst rate (57.56% versus 48.44%), and oocyte utilization rate (52.93% versus 45.29%) were significantly higher in the second ejaculation group (P<0.05). …”
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#146 : Low-Lactate Culture Medium Increases the Percentage of Usable Blastocysts from Advanced Maternal Age Patients: A Sibling-Oocyte, Monocentric Study by Hiromitsu Hattori, Mizuho Takahashi, Mayumi Toya, Hideki Igarashi, Koichi Kyono

“…Embryo development up to Day 6, clinical pregnancy and miscarriage rates were compared. Usable blastocysts were defined as any blastocyst cryopreserved on day 5 or day 6. …”
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The serum oestradiol/progesterone ratio on the day of OPU + 7, but not the day of OPU + 5, affects the rates of live birth in fresh blastocyst embryo transfer cycles by Wenxian Zhao, Honglu Diao, Xin Chen, Shaoyuan Xu, Shengfang Jiang, Hong Cao, Changjun Zhang, Ying Zhang

“…Methods This is a retrospective cohort study of 2,257 women undergoing a follicular-phase depot gonadotropin-releasing hormone agonist (GnRH-a) protocol for in vitro fertilization /intracytoplasmic sperm injection (IVF/ICSI) treatment and fresh blastocyst embryo transfer cycles at a university-affiliated fertility center between January 2016 and April 2021. …”
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Clinical and perinatal outcomes of fresh single-blastocyst-transfer cycles under an early follicular phase prolonged protocol according to day of trigger estradiol levels by Yingfen Ying, Xiaosheng Lu, Huina Zhang, Samuel Kofi Arhin, Xiaohong Hou, Zefan Wang, Han Wu, Jieqiang Lu, Yunbing Tang

Subjects: “…Fresh single blastocyst transfer…”
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#290 : Does Extending the Time Interval Between hCG Trigger and Oocyte Retrieval from 36 to 37 Hours Affect the Rate of Euploid Blastocysts? by Elad Barber, Daniel Lantsberg, Yossi Mizrachi, Rebecca Kelley, Kathryn Gurner, Sharyn Stock-Myer, David Gardner

“…Background and Aims: Data are lacking regarding the optimal time interval between human chorionic gonadotropin (hCG) administration and ovum pick-up (hCG-OPU interval), and its impact on blastocyst euploidy. Our aim was to compare the blastocyst euploidy rate between IVF cycles with hCG-OPU interval of 36 and 37 h. …”
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Mild hypothermia promotes the viability of in vitro-produced bovine blastocysts and their transcriptional expression of the cold-inducible transcription factor Rbm3 during in vitro culture by Toshimichi ISHII, Koga KAWANO, Nobumasa TANAKA, Kensuke TOMITA, Naohiko SAITO, Masayasu YAMADA

Subjects: “…bovine blastocyst…”
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#319 : Blastocyst Formation Prediction Based on Deep Learning Model from 3-Day Embryo Images in Time-Lapse Incubator Using Data Augmentation by Sangho Lee, Kyoung Hee Choi, Sohyun Hwang, Jihyang Kim

“…Background and Aims: Current literature suggests that blastocyst ET (Embryo Transfer) at day 5 improves pregnancy outcomes compared with cleavage ET at day 3. …”
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Day 4 good morula embryo transfer provided compatible live birth rate with day 5 blastocyst embryo in fresh IVF/ET cycles by Ryh-Sheng Li, Yuh-Ming Hwu, Robert Kuo-Kuang Lee, Sheng-Hsiang Li, Ming-Huei Lin

“…Objective: Embryo transfers during cleavage stage (day 2 or day 3) and blastocyst stages (day 5 or day 6) are common in current daily practice in fresh IVF/ET cycles. …”
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The antioxidant capacity of Mito-TEMPO improves the preimplantation development and viability of vitrified-warmed blastocysts through the stabilization of F-actin morphological aspects in bovine embryos by Jae-Hoon Jeong, Hyo-Jin Park, Seul-Gi Yang, Deog-Bon Koo

Subjects: “…bovine blastocyst…”
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Systematic use of long-acting intramuscular progesterone in addition to oral dydrogesterone as luteal phase support for single fresh blastocyst transfer: A pilot study by Virginie Simon, Virginie Simon, Geoffroy Robin, Geoffroy Robin, Laura Keller, Camille Ternynck, Camille Ternynck, Sophie Jonard, Sophie Jonard, Camille Robin, Christine Decanter, Pauline Plouvier

“…Patient, aged between 18 and 43 years old, had received a fresh blastocyst transfer between January 2021 and June 2021. …”
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Comparing blastocyst euploid rates between the progestin-primed and gonadotrophin-releasing hormone antagonist protocols in aneuploidy genetic testing: a randomised trial protocol by Min Yu, He Li, Lu Li, Xiaoxi Sun, Hua Chen, Ernest H Y Ng, Xiang Lu, Wenbi Zhang, Junling Chen

“…This study aims to compare the euploidy rate of blastocysts following the PPOS protocol and the gonadotropin-releasing hormone antagonist protocol in women undergoing preimplantation genetic testing for aneuploidy (PGT-A).Methods/analysis This is a randomised trial. …”
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Successful blastocyst production by intracytoplasmic injection of sperm after in vitro maturation of follicular oocytes obtained from immature female squirrel monkeys (Saimiri boliviensis) by Shinichi WATANABE, Megumi MIURA, Hiromi MORITA, Moeka NISHI, Shin-ichi YOKOTA, Shosaku HATTORI, Hiromichi MATSUMOTO, Emiko FUKUI, Ken Takeshi KUSAKABE, Masanori OCHI, Naomi NAKAGATA, Yasuo KISO, Chieko KAI, Midori YOSHIZAWA

Subjects: “…blastocyst…”
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Generation of sheep with defined FecB B and TBXT mutations and porcine blastocysts with KCNJ5 G151R/+ mutation using prime editing by Shiwei Zhou, Laura Johanna Lenk, Yawei Gao, Yuhui Wang, Xiaoe Zhao, Menghao Pan, Shuhong Huang, Kexin Sun, Peter Kalds, Qi Luo, Simon Lillico, Tad Sonstegard, Ute I. Scholl, Baohua Ma, Bjoern Petersen, Yulin Chen, Xiaolong Wang

“…Additionally, we applied PE to generate porcine blastocysts with a biomedically relevant point mutation (KCNJ5 p.G151R) as a porcine model of human primary aldosteronism. …”
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Developmental potential of mouse single blastomere derived from isolated 2-, 4- and 8- cell embryos into blastocyst and Inner Cell Mass (ICM) uutgrowths by Asdiana Amri, Ramli Abdullah, Wan Khadijah Wan Embong

“…Developmental rate of 2-cell derived SB (77.28±6.77) was greater than 4-cell (63.70±5.35) and 8-cell (55.73±3.35), corresponding to the results of ICM outgrowth at 2-cell (69.29±4.13), gave higher rate followed by 4-cell (55.73±7.81) and 8-cell (41.85±3.58). Diameter of blastocyst decreased as the SB parent embryo stage increased, with the respective ratio of 5 (diameter of SB blastocyst): 3 (diameter of SB) (2-cell: 92.55±1.59 vs. 56.48±0.40; 4-cell: 78.71±1.37 vs. 44.02±0.49 and 8-cell: 64.13±2.20 vs. 35.68±0.34) as well as total cell number in blastocyst (2-cell: 43.00±1.48; 4-cell: 28.33±1.15; 8-cell: 8.80±0.58). …”
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Developmental potential of mouse single blastomere derived from isolated 2-, 4- And 8- Cell embryos into Blastocyst and inner cell mass (ICM) Outgrowths by Amri, A., Abdullah, Ramli, Embong, W.K.W.

“…Developmental rate of 2-cell derived SB (77.28±6.77) was greater than 4-cell (63.70±5.35) and 8-cell (55.73±3.35), corresponding to the results of ICM outgrowth at 2-cell (69.29±4.13), gave higher rate followed by 4-cell (55.73±7.81) and 8-cell (41.85±3.58). Diameter of blastocyst decreased as the SB parent embryo stage increased, with the respective ratio of 5 (diameter of SB blastocyst): 3 (diameter of SB) (2-cell: 92.55±1.59 vs. 56.48±0.40; 4-cell: 78.71±1.37 vs. 44.02±0.49 and 8-cell: 64.13±2.20 vs. 35.68±0.34) as well as total cell number in blastocyst (2-cell: 43.00±1.48; 4-cell: 28.33±1.15; 8-cell: 8.80±0.58). …”

Does ICSI for in vitro fertilization cause more aneuploid embryos? by Xiangli Niu, Jiamin Long, Fangqiang Gong, Weihua Wang

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Effect of Ethanol on Parthenogenetic Activation and α-Tocopherol Supplementation during <i>In Vitro</i> Maturation on Developmental Competence of Summer-Collected Bovine Oocytes by Francisco Báez, Belén Gómez, Victoria de Brun, Nélida Rodríguez-Osorio, Carolina Viñoles

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Effect modification by developmental stage of embryos on the association between late follicular phase progesterone elevation and live birth in fresh transfers by Fei Li, He Cai, Li Tian, Haiyan Bai, Juanzi Shi

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Placental deficiency of the (pro)renin receptor ((P)RR) reduces placental development and functional capacity by Lachlan G. Schofield, Lachlan G. Schofield, Richard G. S. Kahl, Richard G. S. Kahl, Samantha L. Rodrigues, Samantha L. Rodrigues, Joshua J. Fisher, Joshua J. Fisher, Saije K. Endacott, Saije K. Endacott, Sarah J. Delforce, Sarah J. Delforce, Eugenie R. Lumbers, Eugenie R. Lumbers, Jacinta H. Martin, Jacinta H. Martin, Kirsty G. Pringle, Kirsty G. Pringle

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Factors affecting male-to-female ratio at birth in frozen-thawed embryo transfer cycles: a large retrospective cohort study by Tong Du, Qin Xie, Jing Ye, Xindi Wang, Jiaxin Qiu, Zheng Yan, Suqun Zhang, Dong Zhao, Jiaying Lin, Bin Li

Subjects: “…blastocyst transfer…”
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