Gene expression of biopsied murine preimplantation embryos / Norhazlin Jusoh @ Mohd Yusoff by Jusoh @ Mohd Yusoff, Norhazlin

“…Results showed a significant difference in blastocyst development after piezo-assisted biopsy. …”
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Developmental competence of IVF and SCNT goat embryos is improved by inhibition of canonical WNT signaling. by Marjan Sadeghi, Mohsen Rahimi Andani, Mehdi Hajian, Nafiseh Sanei, Reza Moradi-Hajidavaloo, Nasrin Mahvash, Farnoosh Jafarpour, Mohammad Hossein Nasr-Esfahani

“…Initially, we observed cytoplasmic expression of β-CATENIN in 2 and 8-16 cell stage embryos and membranous expression of β-CATENIN in compact morula and blastocyst stages. Furthermore, while we observed exclusively membranous localization of β-catenin in IVF blastocysts, we observed both membranous and cytoplasmic localization in SCNT blastocysts. …”
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#82 : Does Apoptotic Body Impair Pregnancy Outcome by Nur Ilanah Rosli, Najwa Safuhah Mohamad Khir, Nurul Izzah Ab Khalid, Nursyuhada Akma Abdul Kadir, Murizah Mohd. Zain

“…Background: Morphologically suboptimal blastocyst is often associated with poorer pregnancy outcomes. …”
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Developmental competence of IVF and SCNT goat embryos is improved by inhibition of canonical WNT signaling by Marjan Sadeghi, Mohsen Rahimi Andani, Mehdi Hajian, Nafiseh Sanei, Reza Moradi-Hajidavaloo, Nasrin Mahvash, Farnoosh Jafarpour, Mohammad Hossein Nasr-Esfahani

“…Initially, we observed cytoplasmic expression of β-CATENIN in 2 and 8–16 cell stage embryos and membranous expression of β-CATENIN in compact morula and blastocyst stages. Furthermore, while we observed exclusively membranous localization of β-catenin in IVF blastocysts, we observed both membranous and cytoplasmic localization in SCNT blastocysts. …”
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Correlation study of male semen parameters and embryo aneuploidy in preimplantation genetic testing for aneuploidy by Hongyi Yang, Yan Liu, Wenbin Niu, Zilan Yang, Yang Wang, Haixia Jin, Gang Li

“…ObjectiveThe purpose of this study was to evaluate the influence of abnormal semen parameters on embryo aneuploidy based on single nucleotide polymorphism microarray (SNP array).MethodsA total of 464 blastocysts from 103 PGT-A cycles were analyzed. The embryo quality and embryo aneuploidy rates were compared between different groups which divided by male semen parameters (sperm concentration, motility, morphology, and DFI) according the WHO criteria (2021).ResultsThe total blastocysts chromosome aneuploidy rate was 42.3% (191/452). …”
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Assessment of quadrivalent characteristics influencing chromosome segregation by analyzing human preimplantation embryos from reciprocal translocation carriers by Ziravard N. Tonyan, Irina L. Puppo, Alsu F. Saifitdinova, Tatyana V. Vavilova, Andrey S. Glotov

“…Patterns of meiotic chromosome segregation were analyzed in cleavage stage and blastocyst stage human embryos from couples with autosomal reciprocal translocations (ART). …”
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Which Stage of Mouse Embryos Is More Appropriate for Vitrification? by Nasibeh Ghandy, Abbas Ali Karimpur Malekshah

“…During the ensuing days, some of these cultured embryos were vitrified at 4-cell, 8-cell, morula and blastocyst stages. After 10 to 14 days, the embryos were thawed to assess their survival and also cultured to determine the rate of blastocyst formation and hatching. …”
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Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats by Joanna M.G. Souza-Fabjan, Lucas F.L. Correia, Ribrio I.T.P. Batista, Yann Locatelli, Vicente J.F. Freitas, Pascal Mermillod

“…This study evaluated the effect of season on cleavage, blastocyst rates and quality of in vitro produced (IVP) goat embryos. …”
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Embryonic diapause due to high glucose is related to changes in glycolysis and oxidative phosphorylation, as well as abnormalities in the TCA cycle and amino acid metabolism by Jiewei Hong, Hongxuan Tong, Xuan Wang, Xiaoyan Lv, Lijuan He, Xuezhi Yang, Yingli Wang, Kaixia Xu, Qi Liang, Qianjin Feng, Tingli Niu, Xin Niu, Yu Lu

“…The cleavage rate, blastocyst rate and total cell number of blastocyst were used to assess the embryo quality. 40 mM glucose was selected to model high glucose levels in this study. 40mM glucose arrested early embryonic development, and the blastocyst rate and total cell number of the blastocyst decreased significantly as glucose concentration was increased. …”
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#49 : Does Percutaneous Epididymal Sperm Aspiration (PESA)-Sperm Attribute as the Game Changer in High-Risk Male Factor Fertility for Intracytoplasmic Sperm Injection (ICSI)? by Shyam Nandan Gupta, B V Srinivas, Deepak Pandey

“…If >2 IVF failure (with/o > 4 blastocyst transfer), 3. Severe OAT/azoospermia, 4. …”
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Analysis of Morphokinetic Parameters of Feline Embryos Using a Time-Lapse System by Joanna Kochan, Agnieszka Nowak, Barbara Kij, Sylwia Prochowska, Wojciech Niżański

“…Embryos that cleaved very early (17–18 hpi) developed poorly to the blastocyst stage (2%) and none of the embryos that cleaved later than 27 hpi were able to reach the blastocyst stage. …”
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Species and embryo genome origin affect lipid droplets in preimplantation embryos by Paulina Lipinska, Piotr Pawlak, Ewelina Warzych

“…Embryos after IVF/PA were collected at precise time points of development at the following stages: zygote, 2-cell, 4-cell, 8/16-cell, morula, early blastocyst, expanded blastocyst. LD were stained with BODIPY 493/503 dye, embryos were visualized under a confocal microscope and images were analyzed with the ImageJ Fiji software. …”
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Effect of frozen-thawed embryo transfer with a poor-quality embryo and a good-quality embryo on pregnancy and neonatal outcomes by Cheng Zeng, Rui-Hui Lu, Xin Li, Sheng Wang, Yan-Rong Kuai, Qing Xue

“…Thus, the benefits and risks of double blastocyst-stage embryo transfer should be balanced. …”
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The effects of biopsy and cryopreservation by vitrification on the in vitro development of eight-cell mouse embryos by Abdullah, Ramli, Mohamed Said, M.S., Nor Ashikin, M.N.K., Dzulsuhaimi, D., Rajikin, M.H., Mohamed, Z., Faizah, S.

“…Overall, one-blastomere biopsy without cryopreservation yielded the highest rate of development to morula (96.1%), expanded blastocyst (84.3%) and hatched blastocyst (74.5%), respectively. …”

Parthenogenetic development of rabbit oocytes after electrical stimulation by A. Wierzchos

“…Oocytes were cultured until the morula/blastocyst stage (approx. 72 h). The experiment was conducted using 430 oocytes obtained post mortem. …”
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Development of caprine chimeric embryos reconstructed through ES-tetraploid complementation assay by JUHI PATHAK, S D KHARCHE, ANJANA GOEL

“…In conclusion, both the media supported the development of chimeric embryos up to blastocyst.…”
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Multinucleation in the Two-Cell Stage Embryo Matters by Leah LAZZARO, Martin HEALEY, Tiki OSIANLIS

“…Aim: To determine rates of blastocyst utilisation and euploidy for embryos with MN at the two-cell stage (MN2) in comparison to mononucleated and binucleated embryos. …”
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The Impact of Two Embryo Culture Media, Synthetic Oviduct Fluid and Commercial BO, on pre-and post-Implantation Development of Cloned SAANEN Goat Embryos by Mehdi Hajian, Farnoosh Jafarpour, Sayed Morteza Aghamiri, Shiva Rouhollahi Varnosfaderani, Mohsen Rahimi Andani, Mohammad Hossein Nasr-Esfahani

“…All of the examined genes showed increased expression levels in SCNT blastocyst in both media compared to IVF Blastocyst. …”
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Cycle Characteristics and Pregnancy Outcomes of Early Rescue Intracytoplasmic Sperm Injection Cycles in Normal and Hyper-Ovarian Response Women: A Six-Year Retrospective Study by Liang Chen, Hanjing Zhou, Xueli Liu, Jing Zhao, Qianrong Qi, Qingzhen Xie

“…Early r-ICSI delayed blastocyst development as seen by the increase in the number of day 6 blastocysts. …”
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Biopsy Procedure and Preparation (Including Biopsy Medium and Biopsy Dish) by Tiencheng Arthur Chang

“…The workshop emphasizes the significance of procedure and preparation which may impact the blastocyst biopsy and its role in selecting embryos. …”
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