PRD-class homeobox genes in bovine early embryos: function, evolution, and overlapping roles by Lewin, TD, Fouladi-Nashta, AA, Holland, PWH

“…Here, we exploit an ectopic expression approach to examine the role of bovine ETCHbox genes and show that ARGFX and LEUTX homeodomain proteins upregulate genes normally expressed in the blastocyst; the identities of the regulated genes suggest that, in vivo, the ETCHbox genes play a role in coordinating the physical formation of the blastocyst structure. …”

An investigation of the origin and significance of bilateral symmetry of the pronuclear zygote in the mouse. by Gardner, R, Davies, T

“…BACKGROUND: Preliminary observations revealed that advanced zygotes of the PO strain mouse are often bilaterally symmetrical, and suggested that both the plane of first cleavage and features of the blastocyst bear a consistent relationship to the zygote's bilateral plane. …”

Effects of LPA on the development of sheep in vitro fertilized embryos and attempt to establish sheep embryonic stem cells by Xue-min ZHANG, Xiang-hua HUANG, Jing WANG, Ying XING, Fang LIU, Jin-zhu XIANG, Han-ning WANG, Yong-li YUE, Xue-ling LI

“…When the LPA concentration was 15 μmol L–1, the maturation rate, cleavage rate and blastocyst rate decreased significantly (P<0.05), and the blastocyst exhibited abnormal morphology and could not develop into high-quality blastocyst. …”
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Melatonin Improves Quality of Repeated-Poor and Frozen-Thawed Embryos in Human, a Prospective Clinical Trial by Zhongjian Bao, Guangdong Li, Rongxiang Wang, Songguo Xue, Yong Zeng, Shoulong Deng

“…The rate of available blastocysts (17.1% vs.12.7%) and clinical pregnancy rate (25.0% vs.17.1%) were in tendency higher in the group treated with melatonin (P&gt;0.05). …”
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Effect of IGFBP-4 during In Vitro Maturation on Developmental Competence of Bovine Cumulus Oocyte Complexes by Adriana Raquel Camacho de Gutiérrez, Oguz Calisici, Christine Wrenzycki, Juan Carlos Gutiérrez-Añez, Christine Hoeflich, Andreas Hoeflich, Árpád Csaba Bajcsy, Marion Schmicke

“…Cumulus expansion, oocyte maturation, cleavage, blastocyst, and hatching rates were evaluated. Furthermore, blastocyst gene expression of SOCS2, STAT3, SLC2A1, SLCA3, BAX, and POU5F1 transcripts were quantified using RT-qPCR. …”
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DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification by Pablo Bora, Lenka Gahurova, Andrea Hauserova, Martina Stiborova, Rebecca Collier, David Potěšil, Zbyněk Zdráhal, Alexander W. Bruce

“…Successful navigation of the mouse preimplantation stages of development, during which three distinct blastocyst lineages are derived, represents a prerequisite for continued development. …”
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Disruption of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero by Froylan Sosa, Kyungjun Uh, Jéssica N Drum, Katy S Stoecklein, Kimberly M Davenport, M Sofia Ortega, Kiho Lee, Peter J Hansen

“…RNA sequencing of individual morulae and blastocysts indicated a total of 27 (morula) or 15 (blastocyst) differentially expressed genes (false discovery rate <0.05). …”
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#258 : Evaluating the Clinical Utility of Physiological ICSI by Hiroyuki Tomari, Mayu Nagata, Katsuko Kunitake, Keiko Uchimura, Saki Gondo, Kensuke Saito, Yukako Sano, Kou Honjo, Yumi Nagata

“…The evaluation items were embryonic developmental potential and clinical pregnancy and miscarriage rates after single frozen thawed blastocyst transfer. Results: The normal fertilization rates for the conventional and PICSI methods were 81.3% and 80.8%, respectively; the blastocyst rates were 64.7% and 68.2%, respectively; and the good blastocyst rates were 30.8% and 31.8%, respectively. …”
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#279 : The Relationship Between Maternal Age and the Chromosomal Status of the Embryo by Riyan Hari Kurniawan, Raden Muharam, Indah Suci Widyahening, Pritta Ameilia Iffanolida, Budi Wiweko

“…Specifically, advanced maternal age (AMA; defined as [Formula: see text]35 years) shows just a negligible impact upon fertilization rate and a mild impact upon embryo development to the blastocyst stage, but results in a dramatic impact upon blastocyst aneuploidy rate. …”
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The Association between Embryo Development and Chromosomal Results from PGT-A in Women of Advanced Age: A Prospective Cohort Study by Pornchanit Santamonkunrot, Sonsiri Samutchinda, Pornsri Niransuk, Chonthicha Satirapod, Matchuporn Sukprasert

“…Also, there were significantly higher rates of euploid embryos with good blastocyst morphological grades, especially blastocyst expansion grades and trophectoderm grades. …”
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Tauroursodeoxycholic Acid Supplementation in In Vitro Culture of Indicine Bovine Embryos: Molecular and Cellular Effects on the In Vitro Cryotolerance by Elisa Mariano Pioltine, Camila Bortoliero Costa, Fernanda Fagali Franchi, Priscila Helena dos Santos, Marcelo Fábio Gouveia Nogueira

“…There was no increase in the blastocyst and hatched blastocyst rates treated with TUDCA in the IVC. …”
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Active H3K27me3 demethylation by KDM6B is required for normal development of bovine preimplantation embryos by Nhi Chung, Yanina S. Bogliotti, Wei Ding, Marcela Vilarino, Kazuki Takahashi, James L. Chitwood, Richard M. Schultz, Pablo J. Ross

“…Knockdown of maternal KDM6B mRNA inhibited the reduction in global levels of H3K27me3 from 2-cell to 8-cell embryo stages and compromised development to the blastocyst stage; embryos that developed to the blastocyst stage had fewer inner cell mass (ICM) and trophectoderm (TE) cells. …”
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Effervescent Progesterone Vaginal Tablet Mono-Administration Demonstrated Comparable Pregnancy Rate Across the Different Serum Progesterone Levels on the Day of Embryo Transfer in... by Toshihiro Fujiwara, Maki Kusumi, Takafumi Utsunomiya, Mari Nomiyama, Kaoru Yanagida, Yoshitsugu Watanabe, Yukiharu Yamaguchi, Atsushi Tanaka

“…Results: Main outcomes were available for 344 patients, of which 49 in the cleavage stage ET group and 295 in the blastocyst ET group. Ongoing pregnancy rate were 10.2% in cleavage stage ET group and 28.1% in blastocyst ET group. …”
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Comparison between different combinations of chemical treatment on parthenogenetic activation of mouse oocytes and its subsequent embryonic development by Abdullah, Ramli, Rahman, M.M., Idris, S.K., Wan Embong, W.K.

“…Three-hour duration generally showed the highest cleavage (93.83%) and blastocyst rates (60.42%) compared to other durations. …”

Debate 4: Morphological Assessment of Embryos is Outdated <subtitle>Motion For – Author: Tiencheng Arthur Chang; Motion Against – Author: David K. Gardner</subtitle> by Tiencheng Arthur Chang, David K. Gardner

“…Further, at the blastocyst stage it is possible to visualise and quantitate the formation of both the inner cell mass (ICM) and trophectoderm, with all parameters of blastocyst development linked to transfer outcomes. …”
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Neuronal Transplantation for Alzheimer’s Disease and Prospects for Generating Exogenic Neurons as a Source of Cells for Implantation by Phoebe Strell, Sether T. Johnson, Chris Carchi, Walter C. Low

“…Cellular transplantation of healthy exogenic neurons to replace and restore neuronal cell function has previously been explored in AD animal models, yet most of these transplantation methods have utilized primary cell cultures or donor grafts. Blastocyst complementation offers a novel approach to generate a renewable exogenic source of neurons. …”
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Infertility and the Endometrium by Lois A. Salamonsen, Evdokia Dimitriadis

“…In women, the uterus provides the site where the maternal-fetal interface is established and maintained. Final blastocyst development occurs within the uterine cavity, then the blastocyst must attach to and implant into the endometrium (the inner uterine surface), via its outermost trophectodermal cells. …”
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The origin and impact of embryonic aneuploidy. by Fragouli, E, Alfarawati, S, Spath, K, Jaroudi, S, Sarasa, J, Enciso, M, Wells, D

“…This study aimed to shed light on the genesis, progression, and survival of different types of chromosome anomaly from the fertilized oocyte through the final stage of preimplantation development (blastocyst). 2,204 oocytes and embryos were examined using comprehensive cytogenetic methodology. …”

Effects of manganese on maturation of porcine oocytes in vitro and their subsequent embryo development after parthenogenetic activation and somatic cell nuclear transfer by Muhammad QASIM, Jun-Xue JIN, Sanghoon LEE, Anukul TAWEECHAIPAISANKUL, Erif Maha Nugraha SETYAWAN, Geon A KIM, Byeong Chun LEE

“…However, there was no substantial difference in the cleavage rate and total blastocyst cell numbers among all groups. SCNT was performed using the optimal concentration of Mn from PA, which showed an improved blastocyst formation rate in treated oocytes compared to that in control group (P &lt; 0.05). …”
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Influence of fetal calf serum on the production of bovine embryos in vitro by Khanh Van Nguyen, Thi Kim Yen Pham, Thi Au Hoang, Quang Minh Luu, Doan Lan Pham

“…The rate of oocyte cleavage, blastocyst formation, and hatching blastocyst rate of the 0-h group were higher than that of 48-h and 120-h groups (82.96 vs 70.5 and 70.24%; 34.51 vs 24.21 and 23.18%; 10.5 vs 6.12 and 4.13%; respectively, p<0.05). …”
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