Influences of Supplementing Selective Members of the Interleukin-6 Cytokine Family on Bovine Oocyte Competency by Endya McKinley, Savannah L. Speckhart, Jessica A. Keane, Mary A. Oliver, Michelle L. Rhoads, J. Lannett Edwards, Fernando H. Biase, Alan D. Ealy

“…The third study determined that supplementing these cytokines during maturation did not influence fertilization success, but either LIF or IL11 supplementation increased blastocyst development. No effect of IL6 supplementation on subsequent blastocyst development was detected. …”
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Production of Genetically Modified Porcine Embryos via Lipofection of Zona-Pellucida-Intact Oocytes Using the CRISPR/Cas9 System by Celia Piñeiro-Silva, Sergio Navarro-Serna, Ramsés Belda-Pérez, Joaquín Gadea

“…The 5% group had a similar mutant blastocyst rate to the electroporation method (5.52% and 6.38%, respectively, <i>p</i> > 0.05). …”
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Minimal ovarian stimulation combined with elective single embryo transfer policy: age-specific results of a large, single-centre, Japanese cohort by Kato Keiichi, Takehara Yuji, Segawa Tomoya, Kawachiya Satoshi, Okuno Takashi, Kobayashi Tamotsu, Bodri Daniel, Kato Osamu

“…Frozen-thawed blastocyst transfer cycles gave the highest chance of live birth per embryo transfer (41.3 % to 6.1 %).…”
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The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles by Chiyuki KOHATA-ONO, Takuya WAKAI, Hiroaki FUNAHASHI

“…However, the blastocyst formation rate of SF-derived mature oocytes was not affected by the presence of rapamycin. …”
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Comprehensive Transcriptome Analysis of mRNA Expression Patterns of Early Embryo Development in Goat under Hypoxic and Normoxic Conditions by Yongjie Wan, Dongxu Li, Mingtian Deng, Zifei Liu, Liang Liu, Feng Wang

“…In the present study, RNA-seq was performed to compare 8-cell-stage and blastocyst-stage goat embryos under hypoxic and normoxic conditions; zygotes were checked at 72 and 168 h to 8-cell stage (L8C) and blastocyst stage (LM) in hypoxic conditions and 8-cell stage (H8C) and blastocyst stage (HM) in normoxic conditions. …”
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Position- and Hippo signaling-dependent plasticity during lineage segregation in the early mouse embryo by Eszter Posfai, Sophie Petropoulos, Flavia Regina Oliveira de Barros, John Paul Schell, Igor Jurisica, Rickard Sandberg, Fredrik Lanner, Janet Rossant

“…The segregation of the trophectoderm (TE) from the inner cell mass (ICM) in the mouse blastocyst is determined by position-dependent Hippo signaling. …”
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Diminished effect of maternal age on implantation after preimplantation genetic diagnosis with array comparative genomic hybridization by Harton, G, Munné, S, Surrey, M, Grifo, J, Kaplan, B, McCulloh, D, Griffin, D, Wells, D

“…Result(s) Aneuploidy rates increased with maternal age from 53% to 93% for day 3 biopsies and from 32% to 85% for blastocyst biopsies. Implantation rates for euploid embryos for ages &lt;35-42 years did not decrease after PGD: ranges 44%-32% for day 3 and 51%-40% for blastocyst. …”

STUDIES REGARDING THE VIABILITY OF MOUSE EMBRYOS RECOVERED IN EARLY DEVELOPMENTAL STAGES by ALEXANDRA IVAN, N. PĂCALĂ, ADA TELEA, V. CARABĂ

“…The M16 medium assured the embryos development up to the hatched blastocyst stage (5.33%). None of the media supported the development of embryos under 8 cell stages and early blockage in stage of 2 cells could not be prevented.…”
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Double-edged sword: effects of human sperm reactive oxygen species on embryo development in IVF cycles by Jin Liu, Keheng Zhu, Shiyan Xu, Wenjiao Tu, Xiaotan Lin, Youpeng Su, Rong Huang, Yuao Deng, Yu Liu

“…High percentage of sperm mROS and hydrogen peroxide had adverse effects on cleavage-stage embryo and blastocyst development in patients with normo-ovulatory infertility. 2. …”
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Effect of the cryopreservation method used, the embryonic stage and the use of conjugated linoleic acid isomers on the cryotolerance of in vitro-produced bovine embryos by Luciana Simões Rafagnin Marinho, Lain Uriel Ohlweiler, Marcos Henrique Barreta, Paulo Bayard Dias Gonçalves, Joana Claudia Mezzalira, Alceu Mezzalira

“…The embryos were vitrified at the blastocyst (BL) or the expanded blastocyst (EB) stage. …”
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Effect of inducing accessory corpus luteum formation with gonadotropin-releasing hormone or human chorionic gonadotropin on the day of embryo transfer on fertility of recipient dai... by Marcelo S. El Azzi, João Luiz Cardoso, Raul A. Landeo, José Henrique F. Pontes, José C. de Souza, João Paulo N. Martins

“…However, calving/ET in heifers receiving blastocyst fresh IVP embryos was similar among treatments. …”
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Effect of the cryopreservation method used, the embryonic stage and the use of conjugated linoleic acid isomers on the cryotolerance of in vitro-produced bovine embryos by Luciana Simões Rafagnin Marinho, Lain Uriel Ohlweiler, Marcos Henrique Barreta, Paulo Bayard Dias Gonçalves, Joana Claudia Mezzalira, Alceu Mezzalira

“…The embryos were vitrified at the blastocyst (BL) or the expanded blastocyst (EB) stage. …”
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A sister of NANOG regulates genes expressed in pre-implantation human development by Dunwell, T, Holland, P

“…The NANOG homeobox gene plays a pivotal role in self-renewal and maintenance of pluripotency in human, mouse and other vertebrate embryonic stem cells, and in pluripotent cells of the blastocyst inner cell mass. There is a poorly studied and atypical homeobox locus close to the Nanog gene in some mammals which could conceivably be a cryptic paralogue of NANOG, even though the loci share only 20% homeodomain identity. …”

Quantitative analysis of mitochondrial DNA in porcine-mouse cloned embryos by Hyeonyeong Shin, Soyeon Kim, Myungyoun Kim, Jaeeun Lee, Dongil Jin

“…The aim of the research is to identify that porcine oocytes can function as recipients for interspecies cloning and have the ability to develop to blastocysts. Furthermore each mitochondrial DNA (mtDNA) in interspecises cloned embryos was analyzed. …”
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Genome-wide equine preimplantation genetic testing enabled by simultaneous haplotyping and copy number detection by T. De Coster, Y. Zhao, O. Tšuiko, S. Demyda-Peyrás, A. Van Soom, J. R. Vermeesch, K. Smits

“…Two biopsies showed a loss of maternal chromosome 28 and 31, respectively, which were confirmed in the corresponding blastocysts. In one of those biopsies, additional complex aneuploidies not present in the blastocyst were found. …”
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#227 : Is Sperm DNA Fragmentation Assay a Useful Predictor in ART Outcomes? by Shiao Chuan Chua, Steven John Yovich, Peter Michael Hinchliffe, John Lui Yovich

“…Results: The overall rates of fertilization, blastocyst development, good–quality blastocyst, clinical pregnancy, miscarriage and livebirth rates were comparable between the <15% and ≥15% SDF groups. …”
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Improving Sperm Oxidative Stress and Embryo Quality in Advanced Paternal Age Using Idebenone In Vitro—A Proof-of-Concept Study by Victoria Nikitaras, Deirdre Zander-Fox, Nicole O. McPherson

“…Following in vitro fertilization (IVF), embryo development, blastocyst differentiation, DNA damage and cryosurvival, pregnancy and implantation rates and fetal and placental weights were assessed. …”
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#114 : Is Mild Stimulation IVF Better for Women with Previous Poor Outcome Following Conventional High-Dose Stimulation? by Sharmila Thevi Ponusamy, Michelle Sheng Rong Lim, Jang Jih Chen, Pak Seng Wong

“…Results: The fertilisation rate (p=0.00140) and blastocyst utilisation rate (p=0.00966) are significantly higher with MS compared to the HS protocol. …”
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In Vitro Viability and Ultrastructural Changes of Cryopreserved Immature Bovine Oocytes by Myint, Thein

“…Among the initial cooling temperatures tested. 30°C yielded the best maturation (34.4%) and cleavage (4.5%) rates and while maturation, cleavage and blastocyst rates from unfrozen oocytes were 86.7%, 69.5% and 17.4%, respectively. …”
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Nuclear maturation of immature bovine oocytes after vitrification using open pulled straw and cryotop methods by Hajarian, Hadi, Haron, Abd Wahid, Yusoff, Rosnina, Daliri, Morteza, Dashtizad, Mojtaba, R., Holmes, Othman, Abas Mazni

“…The VS solutions significantly reduced zygotic cleavage rate, morulae and blastocyst rates compared with the control group (P < 0.05). …”
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