mTOR hypoactivity leads to trophectoderm cell failure by enhancing lysosomal activation and disrupting the cytoskeleton in preimplantation embryo by Chiyuan Ma, Qin Li, Yuxin Yang, Lei Ge, Jiaxuan Cai, Juan Wang, Maoxian Zhu, Yue Xiong, Wenya Zhang, Jingtong Xie, Yujing Cao, Huashan Zhao, Qing Wei, Chen Huang, Junchao Shi, Jian V. Zhang, Enkui Duan, Xiaohua Lei

“…We discovered that blocking mTOR signaling negatively affected the transformation of 8-cell embryos into blastocysts and caused various deficiencies in blastocyst quality. …”
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Clinical implications of mitochondrial DNA quantification on pregnancy outcomes: a blinded prospective non-selection study by Fragouli, E, McCaffrey, C, Ravichandran, K, Spath, K, Grifo, J, Munné, S, Wells, D

“…All (100%) of these blastocysts had mtDNA levels considered to be normal/low. …”

The why, the how and the when of PGS 2.0: current practices and expert opinions of fertility specialists, molecular biologists, and embryologists by Sermon, K, Capalbo, A, Cohen, J, Coonen, E, De Rycke, M, De Vos, A, Delhanty, J, Fiorentino, F, Gleicher, N, Griesinger, G, Grifo, J, Handyside, A, Harper, J, Kokkali, G, Mastenbroek, S, Meldrum, D, Meseguer, M, Montag, M, Munné, S, Rienzi, L, Rubio, C, Scott, K, Scott, R, Simon, C, Swain, J, Treff, N, Ubaldi, F, Vassena, R, Vermeesch, JR, Verpoest, W, Wells, D, Geraedts, J

“…Most agree that mosaicism is less of an issue at the blastocyst stage than at the cleavage stage but whether mosaicism is no issue at all at the blastocyst stage is currently called into question.…”

#69 : Decline in Mitochondrial Function of Human Embryo with Maternal Aging by Shu Hashimoto, Yoshiharu Morimoto

“…Mitochondrial function at morula stage declined with their maternal age and reduced mitochondrial function decreased the rate of development from morula to blastocyst.…”
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Mitochondrial function of human embryo: Decline in their quality with maternal aging by Shu Hashimoto, Yoshiharu Morimoto

“…Mitochondrial function at morula stage declined with their maternal age and reduced mitochondrial function decreased the rate of development from morula to blastocyst.…”
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Associations of antral follicle count with fertility in cattle: A review by K.J. Alward, R.R. Cockrum, A.D. Ealy

“…Those differences include markers of embryonic viability such as protein level, blastocyst development rates, cleavage rates, and blastocyst cell numbers that differ between high- and low-AFC groups, as well as differential gene expression at the cow and embryo level with genes associated with fertility. …”
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Porcine OCT4 reporter system as a tool for monitoring pluripotency states by Seung-Hun Kim, Chang-Kyu Lee

“…Naïve pluripotent stem cells represented by mouse embryonic stem cells form chimeras after blastocyst injection. Primed pluripotent stem cells represented by mouse epiblast stem cells and human embryonic stem cells. …”
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Biomolecular Markers of Recurrent Implantation Failure—A Review by Aleksandra E. Mrozikiewicz, Marcin Ożarowski, Piotr Jędrzejczak

“…RIF is a complex condition resulting from many physiological and molecular mechanisms involving dynamic endometrium–blastocyst interaction. The most important are the endometrial receptivity process, decidualization, trophoblast invasion, and blastocyst nesting. …”
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Comparison of the effect of two commercialized vitrification carriers on pregnancy outcomes in freeze–thaw cycles by Haibo Zhu, Linlin Li, Hongguo Zhang, Yuting Jiang, Ruizhi Liu, Qi Xi

“…In patients who had blastocyst transplantation, the clinical pregnancy rate of one blastocyst transplanted in those who used OYASHIPS straw was significantly higher than that in those who used J.Y. straw (57.85% vs 47.09%). …”
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#239 : Granulocyte-Macrophage-Colony Stimulating Factor Improve Embryo Development, Mitochondrial Function and Spindle Alignment in a Mouse Model of Advanced Maternal Age by Anmol Saini, Nicole McPherson, Johan Smitz, Mark Nottle

“…Zygotes were cultured in Cleave Media and cleavage and blastocyst rates were determined. Blastocysts were differentially stained on Day 5 to determine cell numbers. …”
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Effect of exposed-to-air frequency of cryopreserved embryo on clinical outcomes of vitrified-warmed embryo transfer cycles: a retrospective analysis of 9,200 vitrified-warmed trans... by Huan Zhang, Danna Ye, Yonggen Wu, Yan Li, Xuefeng Huang

“…Results No significant differences were observed in the positive HCG rate, implantation rate and live birth rate (P > 0.05) among five EAF groups with respect to D3 embryo, D5 blastocyst and D6 blastocyst. Post-warmed survival rate of D3 embryos (P = 0.015) differed significantly among the five EAF groups, but it was not EAF-dependent. …”
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The time interval between laparoscopic tubal ligation and frozen-thawed embryo transfer does not affect the reproductive outcomes by Lijuan Fan, Xiaojuan Li, Juanzi Shi, Haixia Duan

“…Outcomes of cleavage-stage and blastocyst-stage embryo FET were analyzed respectively. …”
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Vitrification of immature bovine cumulus-oocyte complexes: effects of cryoprotectants, the vitrification procedure and warming time on cleavage and embryo development by Prentice-Biensch Jennifer R, Singh Jaswant, Mapletoft Reuben J, Anzar Muhammad

“…</p> <p>Results</p> <p>Cleavage and blastocyst rates did not differ among Control, VS1 and VS1 + VS2 groups in either experiment. …”
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Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes by Jianye LI, Ahmed Zaky BALBOULA, Mansour ABOELENAIN, Takashi FUJII, Satoru MORIYASU, Hanako BAI, Manabu KAWAHARA, Masashi TAKAHASHI

“…Addition of Rapa in good quality COCs did not increase the blastocyst rate, whereas E-64 increased the blastocyst rate and total cell number (TCN) with decreasing TUNEL-positive cells. …”
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What is the Optimum Endometrium Needed for Implantation? by Madhuri Patil

“…A complex process where blastocyst becomes intimately connected with maternal endometrium/decidua and requires; Competent embryo at blastocyst stage Receptive endometrium Synchronized dialogue between maternal and embryonic tissues Endometrial Changes for Implantation include architectural, morphological, cytochemical, molecular and genetic. …”
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Impact of Spermatozoa Cryopreservation on Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Azoospermia: A Retrospective Cross-Sectional Study by Yali Wang, Dongjia Chen, Bing Cai, Dan Huang, Yanwen Xu, Chenhui Ding

“…Results: There were no significant differences in the fertilization, cleavage, good-quality day 3 embryo, blastocyst formation, and good-quality blastocyst rates between the groups. …”
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Advancing bovine in vitro fertilization through 3D printing: the effect of the 3D printed materials by Ramses Belda-Perez, Ramses Belda-Perez, Sonia Heras, Costanza Cimini, Jon Romero-Aguirregomezcorta, Luca Valbonetti, Luca Valbonetti, Alessia Colosimo, Bianca Maria Colosimo, Silvia Santoni, Barbara Barboni, Nicola Bernabò, Nicola Bernabò, Pilar Coy

“…On the other hand, all the extrusion-based processable materials did not cause any detrimental effect on cleavage or blastocyst rates. The principal component analysis reveals that embryos produced in presence of 3D-printed scaffolds produced via extrusion exhibit the highest similarity with the control embryos considering cleavage, blastocyst rates, TCN, ICN/TE and ACR per embryo. …”
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The beneficial effects of antifreeze proteins in the vitrification of immature mouse oocytes. by Jun Woo Jo, Byung Chul Jee, Chang Suk Suh, Seok Hyun Kim

“…The rate of blastocyst formation was significantly higher and the number of caspase-positive blastomeres was significantly lower in the AFP-treated group compared with the untreated group. …”
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Quality assessment of yak oocytes for in vitro production of yak embryos by M HUSSAIN, P CHAKRAVARTY, M S CHAUHAN, S SOREN, B K D BORAH, J DOLEY, P J DAS

“…Good quality oocytes led to embryonic development with significant numbers of transferrable embryos (i.e. morula and blastocyst); 60% of morula was further developed to blastocyst. …”
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Developmental progression continues during embryonic diapause in the roe deer by Anna B. Rüegg, Vera A. van der Weijden, João Agostinho de Sousa, Ferdinand von Meyenn, Hubert Pausch, Susanne E. Ulbrich

“…Abstract Embryonic diapause in mammals is a temporary developmental delay occurring at the blastocyst stage. In contrast to other diapausing species displaying a full arrest, the blastocyst of the European roe deer (Capreolus capreolus) proliferates continuously and displays considerable morphological changes in the inner cell mass. …”
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