Effects of microvibration stimulation on developmental potential of discarded germinal vesicle oocytes of human by Qinli Liu, Sen Zhao, Jian Zhou, Ping Liu, Bo Huo

“…We investigated the differences between the two groups in terms of oocyte maturation rate, parthenogenetic activation rate, and parthenogenetic blastocyst formation rate.ResultsThe static culture and 10 Hz microvibration culture of 95 oocytes showed that the parthenogenetic blastocyst formation rate in the microvibration culture group was significantly higher than those in the traditional static culture group.ConclusionA continuous microvibration stimulation can significantly improve the parthenogenetic developmental potential of human immature oocytes.…”
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Relationship between Nucleus Swelling and Development Competence of Bovine Cloned Embryos Reconstructed by Enucleated Oocytes with Serum-starved or Serum-fed Fetal Somatic Cells by Mokhamad Fahrudin, Ni Wayan Kurniani Karja, Tatsuyuki Suzuki

“…Whereas, the proportion of reconstructed embryos that<br />developed to the morula and blastocyst stages were not significantly different among the groups. …”
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The Improvement of Porcine In Vitro Embryo Development through Regulating Autophagy by miRNA-143 Inhibition by Ridlo, Muhammad Rosyid, Kim, Eui Hyun, Kim, Eun Pyo, Kim, Geon A.

“…We evaluated rates of cleavage, blastocyst, and total cell number of blastocyst; additionally, we performed LC3 immunofluorescence analysis and mRNA expression analyses of genes associated with autophagy, endoplasmic reticulum (ER)-phagy, ER stress, embryo quality, and apoptosis. …”
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The role of preimplantation genetic diagnosis in diagnosing embryo aneuploidy. by Munné, S, Howles, C, Wells, D

“…Recent data suggest that biopsy at the blastocyst stage may allow sampling of representative genetic material without compromising embryo viability. …”

Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease by Hyslop, LA, Blakeley, P, Craven, L, Richardson, J, Fogarty, NME, Fragouli, E, Lamb, M, Wamaitha, SE, Prathalingam, N, Zhang, Q, O'Keefe, H, Takeda, Y, Arizzi, L, Alfarawati, S, Tuppen, HA, Irving, L, Kalleas, D, Choudhary, M, Wells, D, Murdoch, AP, Turnbull, DM, Niakan, KK, Herbert, M

“…This promotes efficient development to the blastocyst stage with no detectable effect on aneuploidy or gene expression. …”

Optimising Electroporation Condition for CRISPR/Cas-Mediated Knockout in Zona-Intact Buffalo Zygotes by Meeti Punetha, Dharmendra Kumar, Sheetal Saini, Suman Chaudhary, Kamlesh Kumari Bajwa, Surabhi Sharma, Manu Mangal, Prem S. Yadav, Jonathan A. Green, Kristin Whitworth, Tirtha K. Datta

“…To elucidate the effect of POU5F1-KO on other pluripotent genes, we determined the relative expression of SOX2, NANOG, and GATA2 in the control (POU5F1 intact) and POU5F1-KO-confirmed blastocyst. POU5F1-KO significantly (<i>p</i> ≤ 0.05) altered the expression of SOX2, NANOG, and GATA2 in blastocyst stage embryos. …”
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Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation Secreção de interferon-tau em embriões bovinos produzidos in vitro frescos e congel... by M.C.C. Araújo, V.R. Vale Filho, A.M. Ferreira, W.F. Sá, J.B. Barreto Filho, L.S.A. Camargo, R.V. Serapião, M.V.G.B. Silva

“…After reaching the blastocyst phase, the embryos were kept on individual culture for additional period of 7 days. …”
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Zona Opening - The Different Options and Methods by Sweelian Liow

“…In blastocyst biopsy, zona opening is an essential step to facilitate extraction of a few trophectodermal cells for analysis. …”
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Cell Transplantation for Repair of the Spinal Cord and Prospects for Generating Region-Specific Exogenic Neuronal Cells by Alex Roman, Anne Huntemer-Silveira, Madison A. Waldron, Zainab Khalid, Jeffrey Blake, Ann M. Parr, Walter C. Low

“…A potential source of spinal cord cells for transplantation is the generation of exogenic neuronal progenitor cells via the emerging technologies of gene editing and blastocyst complementation. This review highlights the use of cell transplantation to treat SCI in the context of relevant developmental gene expression patterns useful for producing regionally specific exogenic spinal cells via in vitro differentiation and blastocyst complementation.…”
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Maternal body mass index is not associated with assisted reproductive technology outcomes by Nobuyuki Kidera, Tomonori Ishikawa, Toshihiro Kawamura, Naoyuki Miyasaka

“…Multiple regression analysis on normal fertilization and high-grade blastocyst rates revealed no statistically significant differences. …”
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iSCNT embryo culture system for restoration of Cervus nippon hortulorum, presumed to be sika deer in the Korean Peninsula. by Yong-Su Park, Min-Gee Oh, Sang-Hwan Kim

“…In addition, the stimulation of endometrial cells and progesterone in the IVC system expanded the blastocyst cavity and enabled stable development of energy metabolism and morphological changes in the blastocyst. …”
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Live birth rates after resolution of endometrial cavity fluid in frozen embryo transfer cycles by Vincent Nguyen, Aaron Jackson, Jenna Gale

“…Results A total of 1034 frozen blastocyst transfer cycles were included, 54 with resolved ECF and 980 without ECF. …”
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Laboratory characteristics of blastocystis invasion in patients with chronic viral hepatitis by A. S. Sigidaev, V. S. Sukachev, M. V. Kurtukov, K. V. Zhdanov, S. S. Kozlov, K. V. Kozlov, A. V. Laskin, E. A. Tarasova, M. A. Suvorova

“…Based on the survey results in 1322 patients found that patients with hronic viral hepatitis (CVH) blastocysts are found more often than in the group of individuals without concomitant pathology of the hepatic-biliary system (28.8% vs. 5.5%, respectively). …”
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Paternal genome rescues mouse preimplantation embryo development in the absence of maternally-recruited EZH2 activity by Huili Wang, Erika E Paulson, Libing Ma, Pablo J Ross, Richard M Schultz

“…EZH2 is encoded by a dormant maternal mRNA and inhibiting the maturation-associated increase in EZH2 activity using either a combined siRNA/morpholino approach or a small molecule inhibitor (GSK343) inhibits development of diploidized parthenotes to the blastocyst stage but not inseminated eggs, with longer GSK343 treatments leading to progressively greater inhibition of development. …”
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Karyomapping: A single centre's experience from application of methodology to ongoing pregnancy and live-birth rates by Ben-Nagi, J, Wells, D, Doye, K, Loutradi, K, Exeter, H, Drew, E, Alfarawati, S, Naja, R, Serhal, P

“…Of 608 embryos, 146 (24%, 95% CI 21–28) day-3 and 462 (76%, 95% CI 72–79) blastocyst biopsies were performed. A total of 81 embryo transfers were performed; 16/81 (20%) were following day-3 embryo biopsy, 65/81 (80%) were following blastocyst biopsy and cryopreserved embryo transfer. …”

Theophylline Enhances In Vitro Fertilization and Embryo Production in Buffaloes by I. Akter, Md. Hasanur Alam, M. N. Islam, A. K. Modak, A. Khatun, M. Moniruzzaman

“…The morula and blastocyst formation percentages were higher in the groups treated with 2.5 mM theophylline than in the control groups. …”
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Functional HLA-C expressing trophoblast spheroids as a model to study placental–maternal immune interactions during human implantation by Marina Alexandrova, Diana Manchorova, Yuan You, Gil Mor, Violeta Dimitrova, Tanya Dimova

“…These models resemble the hatched human blastocyst in size, shape and function and are useful for in vitro studies of the in vivo concealed human implantation. …”
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The Effects of LeukemiaInhibitory Factor onDevelopment of MousePreimplantation Embryo by I Amiri, M Parvini, A Amini, R Mahmodi

“…However, it has positive effects on preimplantation blastocyst growth, differentiation and hatching.…”
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The Different Biopsy Techniques by Keshav Malhotra

“…Trophectoderm Biopsy (Day 5-7 Biopsy) : —Performed at the blastocyst stage, typically on the fifth or sixth day of embryonic development. …”
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Maternal Neutrophil Depletion Fails to Avert Systemic Lipopolysaccharide-Induced Early Pregnancy Defects in Mice by Sourav Panja, John T. Benjamin, Bibhash C. Paria

“…Maternal infection-induced early pregnancy complications arise from perturbation of the immune environment at the uterine early blastocyst implantation site (EBIS), yet the underlying mechanisms remain unclear. …”
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