How Mechanical Forces Change the Human Endometrium during the Menstrual Cycle in Preparation for Embryo Implantation by Anna K. Sternberg, Volker U. Buck, Irmgard Classen-Linke, Rudolf E. Leube

“…Accumulating evidence has shown that junctional adhesion, cytoskeletal rearrangement, and extracellular matrix stiffness affect the local force balance that regulates endometrial differentiation and blastocyst invasion. A focus of this review is on the hormonal regulation of endometrial epithelial cell mechanics. …”
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Superovulatory responsiveness and embryonic development in Iranian Afshari ewes treated with two different concentrations of bovine somatotropin by AMIR HOSSEIN ASGARI SAFDAR

“…Number of transferable embryos and embryos that had reached to the blastocyst in bST-50 was more than bST-100 and control group. …”
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Causes and estimated incidences of sex-chromosome misdiagnosis in preimplantation genetic diagnosis of aneuploidy by Ravichandran, K, Guzman, L, Escudero, T, Zheng, X, Colls, P, Jordan, A, Cohen, J, Wells, D, Munné, S

“…The reported misdiagnosis rates suggest that PGD-A for sex determination is highly accurate, particularly when using aCGH applied to blastocyst biopsies.…”

Small Noncoding RNA Signatures for Determining the Developmental Potential of an Embryo at the Morula Stage by Angelika Timofeeva, Yulia Drapkina, Ivan Fedorov, Vitaliy Chagovets, Nataliya Makarova, Maria Shamina, Elena Kalinina, Gennady Sukhikh

“…Predicted gene targets of these sncRNAs included those globally decreased at the 8-cell–morula–blastocyst stage and critical to early embryo development. …”
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Protein O-GlcNAcylation Promotes Trophoblast Differentiation at Implantation by Peter T. Ruane, Cheryl M. J. Tan, Daman J. Adlam, Susan J. Kimber, Daniel R. Brison, John D. Aplin, Melissa Westwood

“…Embryo implantation begins with blastocyst trophectoderm (TE) attachment to the endometrial epithelium, followed by the breaching of this barrier by TE-derived trophoblast. …”
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Sperm-borne microRNA-34c regulates maternal mRNA degradation and preimplantation embryonic development in mice by Long Cui, Li Fang, Lili Zhuang, Biwei Shi, Chao-Po Lin, Yinghui Ye

“…The embryonic development of the microinjected zygotes was evaluated, and the messenger RNA (mRNA) expression profiles of the embryos at the two-cell, four-cell and blastocyst stages (five embryos per group) were determined by RNA sequencing analysis. …”
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Increased Krüppel-like factor 12 in recurrent implantation failure impairs endometrial decidualization by repressing Nur77 expression by Chenyang Huang, Yue Jiang, Jianjun Zhou, Qiang Yan, Ruiwei Jiang, Xi Cheng, Jun Xing, Lijun Ding, Jianxin Sun, Guijun Yan, Haixiang Sun

“…Gene expression regulation was examined by real-time-quantitative PCR (qRT-PCR), immunostaining and luciferase reporter assay. Further, blastocyst-like spheroid (BLS) and blastocyst implantation models were performed to examine the roles of KLF12 and Nur77 during embryo expansion on hESCs. …”
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Beneficial Effects of Melatonin in the Ovarian Transport Medium on In Vitro Embryo Production of Iberian Red Deer (<i>Cervus elaphus hispanicus</i>) by Irene Sánchez-Ajofrín, María Iniesta-Cuerda, Patricia Peris-Frau, Alicia Martín-Maestro, Daniela-Alejandra Medina-Chávez, Carolina Maside, María Rocío Fernández-Santos, José Antonio Ortiz, Vidal Montoro, José Julián Garde, Ana Josefa Soler

“…Moreover, the percentage of cleavage and blastocyst yield in red deer was greater compared to the Control group and there was lower abundance of oxidative stress- and apoptosis-related <i>SHC1</i>, <i>TP53</i>, and <i>AKR1B1</i> mRNA transcripts in blastocysts for the Melatonin group. …”
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Female obesity increases the risk of preterm birth of single frozen-thawed euploid embryos: a retrospective cohort study by Xitong Liu, Juanzi Shi

“…As preimplantation genetic testing for aneuploidies (PGT-A) is increasingly used worldwide, however, it is still unclear whether body mass index (BMI) has an effect on the preterm birth rate in patients undergoing in vitro fertilization (IVF) with PGT-A when transferring a single euploid blastocyst.Materials and methods This retrospective, single-center cohort study included 851 women who underwent the first cycle of frozen-thawed single euploid blastocyst transfer with PGT-A between 2015 and 2020. …”
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Cryopreservation and Transfer of Sheep Embryos Recovered at Different Stages of Development and Cryopreserved Using Different Techniques by Marina I. Selionova, Magomet M. Aibazov, Ekaterina K. Zharkova

“…Differences in cryoresistance between embryonic developmental stages in favor of the morula/blastocyst stage were found (survival after thawing 86.4% vs. 75.0% in early embryos). …”
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The Improvement of Porcine In Vitro Embryo Development through Regulating Autophagy by miRNA-143 Inhibition by Muhammad Rosyid Ridlo, Eui Hyun Kim, Eun Pyo Kim, Geon A. Kim

“…We evaluated rates of cleavage, blastocyst, and total cell number of blastocyst; additionally, we performed LC3 immunofluorescence analysis and mRNA expression analyses of genes associated with autophagy, endoplasmic reticulum (ER)-phagy, ER stress, embryo quality, and apoptosis. …”
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The Mare: A Pertinent Model for Human Assisted Reproductive Technologies? by Achraf Benammar, Emilie Derisoud, François Vialard, Eric Palmer, Jean Marc Ayoubi, Marine Poulain, Pascale Chavatte-Palmer

“…Embryos are transferred only as blastocysts in horses. In contrast, four cells to blastocyst stage embryos are transferred in humans. …”
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#242 : Correlation of Sperm DNA Fragmentation with Laboratory Performance in Good and Poor Prognosis Patient Groups at Morula IVF Jakarta by Nurul Hikmawati, Pitra Rahmawati, Arief Boediono, Ivan Rizal Sini

“…CONCLUSIONS: Sperm DNA fragmentation rate > 14.9% correlated with fifth-day embryo quality and a lower blastocyst rate in the good prognosis group.…”
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IGF-I Medium Supplementation Improves Singly Cultured Cat Oocyte Maturation and Embryo Development In Vitro by Lorena Fernandez-Gonzalez, Valeria Kozhevnikova, Eugeny Brusentsev, Stefanie Jänsch, Sergei Amstislavsky, Katarina Jewgenow

“…The results showed that the addition of IGF-I and/or GM-CSF produced an increase in morula and blastocyst rate in a single culture system. In particular, the supplementation with 20 ng/mL of IGF-I incremented the maturation rate by 10% and significantly increased the morula and blastocyst rates in single culturing. …”
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Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization by Shivani Malpotra, Pallavi Goel, Songyukta Shyam, Manoj Kumar Singh, Prabhat Palta

“…The data generated from MeDIP-Seq was validated at blastocyst stage cloned and IVF embryos by bisulfite-sequencing PCR on five randomly selected gene regions.…”
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A meta-analysis: Effect of androgens on reproduction in sows by Zhenhua Guo, Lei Lv, Lei Lv, Di Liu, Hong Ma, Cedomir Radovic

“…T did not have a relevant effect on swine in vivo blastocyst survival rate. DHT had a negative phase with respect to blastocyst survival rate. …”
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Paracrine interactions through FGFR1 and FGFR2 receptors regulate the development of preimplantation mouse chimaeric embryo by Katarzyna Krawczyk, Katarzyna Wilczak, Katarzyna Szczepańska, Marek Maleszewski, Aneta Suwińska

“…This finding suggests that FGF signalling is a key part of the regulatory mechanism that assigns cells to a given lineage and ensures the proper composition of the blastocyst, which is a prerequisite for its successful implantation in the uterus and for further development.…”
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Polarity inversion reorganizes the stem cell compartment of the trophoblast lineage by Hatice O. Ozguldez, Niraimathi Govindasamy, Rui Fan, Hongyan Long, Karina Mildner, Dagmar Zeuschner, Britta Trappmann, Adrian Ranga, Ivan Bedzhov

“…Summary: The extra-embryonic tissues that form the placenta originate from a small population of trophectoderm cells with stem cell properties, positioned at the embryonic pole of the mouse blastocyst. During the implantation stages, the polar trophectoderm rapidly proliferates and transforms into extra-embryonic ectoderm. …”
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#78 : Can Short-Term Insemination with Cumulus Reduction Improve IVF Success? by Jessica Morris

“…Conclusion: This study reports an increased number of blastocysts, and good quality utilisable day 5 blastocysts when using the STI protocol, with no negative impact to results.Future studies should include the analysis of cumulative pregnancy rates, and live birth rates.…”
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Roles of Resveratrol in Improving the Quality of Postovulatory Aging Oocytes In Vitro by Yan-Li Sun, Shou-Bin Tang, Wei Shen, Shen Yin, Qing-Yuan Sun

“…With 1.0 µM resveratrol treatment during aging process, the rates of fertilization and blastocyst in POA oocytes increased significantly compared with those in the POA group. …”
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