Psychological stress on female mice diminishes the developmental potential of oocytes: a study using the predatory stress model. by Yu-Xiang Liu, Ya-Nan Cheng, Yi-Long Miao, De-Li Wei, Li-Hua Zhao, Ming-Jiu Luo, Jing-He Tan

“…In vitro experiments showed that whereas oocyte maturation and Sr(2+) activation or fertilization were unaffected by maternal predatory stress, rate of blastocyst formation and number of cells per blastocyst decreased significantly in stressed mice compared to non-stressed controls. …”
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Gonadotropin releasing hormone antagonist protocol brings about more available embryos than agonist long protocol by ZHAO Shan-ke, FU Yao, CHU Da-peng, FU Lei, HAN Shuo, BAI Xue-yan, LI Yuan, ZHOU Wen-hui

“…Results Antagonist group displayed significantly higher available embryo rate (29.94% vs 31.59%), good-quality two-pronuclear(2PN) embryo rate (44.67% vs 46.86%), available blastocyst/OPU(ovum pick-up)(1±2 vs 1±2) and available blastocyst rate (25.62% vs 29.66%) than the long protocol group. …”
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Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device by Kenji Momozawa, Atsushi Matsuzawa, Yukio Tokunaga, Shiori Abe, Yumi Koyanagi, Miho Kurita, Marina Nakano, Takao Miyake

“…Methods In Experiment 1, blastocysts were vitrified using the KVS. Vitrified blastocysts were warmed and subsequently cultured for 72 h. …”
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Effect of Intracytoplasmic Sperm Injection (ICSI) on Mouse Embryos Preimplantational Development by Claudia Cârste, Alexandru T. Bogdan, Judith Ipate, Ada Cean, Daniela Ilie, Roxana Vintilă

“…We compared the development of mouse blastocysts produced by intracytoplasmic sperm injection (ICSI) versus blastocysts fertilized in vivo and cultured in vitro from the zygote stage (IVC). …”
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The Anti-Apoptotic Role of Berberine in Preimplantation Embryo In Vitro Development through Regulation of miRNA-21. by Chao Zhang, Ya-Ran Shi, Xiao-Ran Liu, Yong-Chun Cao, Di Zhen, Zi-Ye Jia, Jin-Qi Jiang, Jian-Hui Tian, Jian-Ming Gao

“…The in vitro developmental rates of 2- and 4-cell embryos and blastocysts, blastocyst cell numbers, apoptotic rates, and apoptotic cell numbers were measured in each group. …”
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Fatty acid supplementation into warming solutions improves pregnancy outcomes after single vitrified‐warmed cleavage stage embryo transfers by Ayumi Amagai, Kenji Ezoe, Tetsuya Miki, Kiyoe Shimazaki, Tadashi Okimura, Keiichi Kato

“…The morphokinetics during cleavage, morula, and blastocyst stages were comparable between the groups. …”
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Occurrence of late-apoptotic symptoms in porcine preimplantation embryos upon exposure of oocytes to perfluoroalkyl substances (PFASs) under in vitro meiotic maturation by Anna Leclercq, Petter Ranefall, Ylva Cecilia Björnsdotter Sjunnesson, Ida Hallberg

“…Higher cell count was associated with lower proportion of apoptotic cells, i.e., larger blastocysts contained less apoptotic cells. Upon PFAS exposure during IVM, PFHxS tended to result in higher blastocyst rates on day 5 post fertilization (p = 0.07) and on day 6 post fertilization (p = 0.05) as well as in higher apoptosis rates in blastocysts (p = 0.06). …”
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Comparisons of conventional in vitro fertilization versus intracytoplasmic sperm injection in women with thyroid autoimmunity and non-male factor infertility, a propensity score ma... by Yuchao Zhang, Yanli Liu, Chunyan Shen, Yichun Guan

“…The good cleavage embryo rate, blastocyst utilization rate, and good blastocyst rate were significantly lower in the intracytoplasmic sperm injection group than those in the conventional in vitro fertilization group. …”
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Matched Developmental Timing of Donor Cells with the Host Is Crucial for Chimera Formation by Markoulaki, Styliani, Cohen, Malkiel A, Jaenisch, Rudolf

“…Chimeric mice have been generated by injecting pluripotent stem cells into morula-to-blastocyst stage mouse embryo or by introducing more mature cells into later stage embryos that correspond to the differentiation stage of the donor cells. …”
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Array comparative genomic hybridization screening in IVF significantly reduces number of embryos available for cryopreservation. by Liu, J, Sills, E, Yang, Z, Salem, SA, Rahil, T, Collins, G, Liu, X, Salem, R

“…In group B, 389 good morphology blastocysts were identified and a single top quality blastocyst was selected for fresh transfer. …”

The Metabolic Signature of In Vitro Produced Bovine Embryos Helps Predict Pregnancy and Birth after Embryo Transfer by Isabel Gimeno, Pablo García-Manrique, Susana Carrocera, Cristina López-Hidalgo, Luis Valledor, David Martín-González, Enrique Gómez

“…Day-6 embryos were developed in single CM (modified synthetic oviduct fluid) for 24 h and transferred to recipients as fresh (28 ETs) or frozen/thawed (58 ETs) Day-7 blastocysts. Variability was induced with seven bulls, slaughterhouse oocyte donors, culture conditions (serum + Bovine Serum Albumin [BSA] or BSA alone) prior to single culture embryonic stage records (Day-6: morula, early blastocyst, blastocyst; Day-7: expanding blastocyst; fully expanded blastocysts) and cryopreservation. …”
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Initial ovarian sensitivity index predicts embryo quality and pregnancy potential in the first days of controlled ovarian stimulation by David Camargo-Mattos, Uziel García, Felipe Camargo-Diaz, Ginna Ortiz, Ivan Madrazo, Esther Lopez-Bayghen

“…Results MOSI could predict the production of ≥4 high-quality embryos by Day 2 (AUC = 0.69, 95%CI:0.63–0.75), ≥2 blastocysts (AUC = 0.74, 95%CI:0.68–0.79), and ≥ 35% rate of blastocyst formation (AUC = 0.65, 95%CI:0.58–0.72). …”
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Progestin Primed Ovarian Stimulation (PPOS) protocol yields lower euploidy rate in older patients undergoing IVF by Angel Hsin-Yu Pai, Yen Ju Sung, Chia-Jung Li, Chieh- Yu Lin, Chia Lin Chang

“…Results Patients who underwent PPOS treatment had significantly reduced blastocyst formation rate and euploidy rate compared to those who received the GnRH antagonist protocol. …”
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Effects of exogenous corticosterone on in vivo fertilizing capacity of epididymal sperm and reversal of its adverse impactbytocotrffinol in the rat / Noor Azean Anis Abd Aziz by Abd Aziz, Noor Azean Anis

“…Laparotomy was performed on day 8 post-coitus to determine the number of blastocyst implantation. Pregnant animals were left until term. …”
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The Timing of Vitrification After Biopsy by Kenji Ezoe

“…The blastocysts are often vitrified after trophectoderm biopsy to secure the time for transportation to external facilities and diagnosis. …”
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In vivo culture of bovine embryos and quality assessment of in vivo vs. in vitro produced embryos by V. Havlicek, M. Lopatarova, S. Cech, R. Dolezel, T. Huber, A. Pavlok, G. Brem, U. Besenfelder

“…After flushing at day seven, 106 blastocysts (blastocyst rate: 13.6% ) were found. The additional 24 h of in vitro culture (day eight) resulted in 153 blastocysts (blastocyst rate: 19.6% ). …”
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Establishment of a Wisent (<i>Bison bonasus</i>) Germplasm Bank by Anna Maria Duszewska, Magdalena Baraniewicz-Kołek, Jarosław Wojdan, Katarzyna Barłowska, Wojciech Bielecki, Paweł Gręda, Wojciech Niżański, Wanda Olech

“…This study obtained embryos in the morula and early blastocyst stages only after oocytes were fertilized and matured for 30 h. …”
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The incidence and origin of segmental aneuploidy in human oocytes and preimplantation embryos by Babariya, D, Fragouli, E, Alfarawati, S, Spath, K, Wells, D

“…A total of 3541 samples comprising of 452 human oocytes, 1762 cleavage stage and 1327 blastocyst stage embryos were investigated in the present study.…”

Effects of S-adenosyl-L-homocysteine and trichostatin A on developmental competence, epigenetic modification, and gene expression in cloned cattle embryos by Jafari, Shahram

“…The results of this study showed TSA treatment of SCNT embryos for 12 h after activation, significantly (P<0.05) improve blastocysts rate. A significant reduction and increase (P<0.05) were observed in analyse of DNA methylation and histone acetylation levels respectively in the blastocyst stage of the TSA-treated group which is similar to IVF-derived blastocysts. …”
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Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor by T. Krejčová, J. Petr, M. Krejčová, K. Kheilová

“…A significantly higher proportion of developing parthenogenetic embryos was observed after the pulsatile treatment (development to the morula stage 0 vs. 18%; development to the blastocyst 0 vs. 7%; P &lt; 0.05). However, this developmental rate was significantly lower (P &lt; 0.05) than the development induced by conventional activation treatment with calcium ionophore (development to the morula stage, 23%; development to the blastocyst stage, 18%). …”
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