Maslinic Acid Supplementation during the In Vitro Culture Period Ameliorates Early Embryonic Development of Porcine Embryos by Regulating Oxidative Stress by Ting-Ting Yang, Jia-Jia Qi, Bo-Xing Sun, He-Xuan Qu, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Shuang Liang

“…Our results showed that 1 μM was the optimal concentration of MA, which resulted in dramatically increased blastocyst formation rates and improvement of blastocyst quality of <i>in vitro</i>-derived embryos from parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT). …”
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Growth factors and lipid transport in early mammalian development by Brice, A

“…The addition of IGF-I and IGF-II to early cleavage stage mouse embryos <em>in vitro</em> did not influence their growth rate to the blastocyst stage; they neither affected cell numbers nor cell doubling. …”

Sex differences in rat placental development: from pre-implantation to late gestation by Kalisch-Smith, J, Simmons, D, Pantaleon, M, Moritz, K

“…We hypothesised that sexual dimorphism begins at the blastocyst stage with sex differences in the progenitor trophectoderm (TE) and its derived trophoblast lineages, as these cells populate the majority of cell types within the placenta. …”

Effect of equilibration temperature on in vitro viability and subsequent embryo development of vitrified-warmed immature bovine oocytes by Hajarian, Hadi, Haron, Abd Wahid, Othman, Abas Mazni, Yusoff, Rosnina, Daliri, Morteza, Dashtizad, Mojtaba, Abood Haris, Faizah, Yap, Chee Kong, Jaffar, Fahrul Firdaus, Ann, Fazly

“…Cleavage rate of 37°C group (38.77%) was greater than other groups (30.84 and 28.95% for 32 and 41°C, respectively). The highest blastocyst rate was also produced when oocytes equilibrated at 37°C (6.45%). …”
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Micromanipulator Set-up - Taking Control of This System by Ryan Rose

“…In this session attendees will delve into the practical facets of setting up and controlling a micromanipulator within the context of a blastocyst biopsy. The session will also cover the important aspects of system set-up, instrument alignment, and objective positioning, aimed to maximize control, precision, and stability during the biopsy procedure. …”
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Making a commitment: cell lineage allocation and axis patterning in the early mouse embryo. by Arnold, S, Robertson, E

“…Recent advances have uncovered details of the molecular circuits that tightly control cell growth and differentiation in the mammalian embryo from the blastocyst stage, through the establishment of initial anterior-posterior polarity, to gastrulation, when the germ cells are set aside and the three primary germ layers are specified. …”

Occurrence and greater intensity of estrus in recipient lactating dairy cows improve pregnancy per embryo transfer by A.M.L. Madureira, T.A. Burnett, J.C.S. Marques, A.L. Moore, S. Borchardt, W. Heuwieser, T.G. Guida, J.L.M. Vasconcelos, C.F. Baes, R.L.A. Cerri

“…Cows receiving embryos in the early blastocyst and blastocyst stage had greater fertility compared with cows receiving embryos in the morula stage. …”
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Effect of calcium ionophore (A23187) on embryo development and its safety in PGT cycles by Junya Zhang, Junya Zhang, Guidong Yao, Guidong Yao, Tongwei Zhang, Tongwei Zhang, Jingyi Hu, Jingyi Hu, Guang Yang, Guang Yang, Jiahuan He, Jiahuan He, Qina He, Qina He, Huiying Fan, Huiying Fan, Yucheng Bai, Yucheng Bai, Yingpu Sun, Yingpu Sun

“…Furthermore, no significant differences were observed between the two groups in terms of the live birth rate (43.75% vs. 52.99%), week of delivery, and birth weight of the infants after transfer of euploid blastocysts (p &gt; 0.05). Furthermore, the 2PN rate, oocyte cleavage rate, blastocyst formation rate, and live birth rate were found to be significantly lower in the A-ICSI group than those in the ICSI group (p &lt; 0.01), but there was no significant difference between the two groups in the week of delivery and birth weight of live births (p &gt; 0.05).DiscussionThese results suggest that the use of calcium ionophore (A23187) activation as an option in cases of ICSI fertilization failure does not affect the ploidy of developing blastocysts and has no significant effects on the week of delivery or birth weight after transfer. …”
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Derivation of human embryonic stem cell line Genea022 by Biljana Dumevska, Alexis Bosman, Robert McKernan, Uli Schmidt, Teija Peura

“…The Genea022 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. …”
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Derivation of Genea047 human embryonic stem cell line by Biljana Dumevska, Omar Chami, Robert McKernan, Divya Goel, Uli Schmidt

“…The Genea047 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated human feeders. …”
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Chromosomal analysis of 262 miscarried conceptuses: a retrospective study by Juan Gui, Jinli Ding, Tailang Yin, Qian Liu, Qingzhen Xie, Lei Ming

“…Results The rate of chromosomal abnormality in the fresh embryo transfer group and the cleavage embryo transfer group was significantly higher than that in the frozen embryo transfer group (79.2% vs. 36%, P = 0.0001) and the blastocyst transfer group (66.7% vs. 32.1%, P = 0.0001) respectively. …”
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Effects of reduced glutathione supplementation in semen freezing extender on frozen-thawed bull semen and in vitro fertilization by Kazuko OGATA, Akira IMAI, Shinya SATO, Kagetomo NISHINO, Shinya WATANABE, Tamas SOMFAI, Eiji KOBAYASHI, Kumiko TAKEDA

“…Two of them showed both improved cleavage and blastocyst formation rates, while the other one only showed an improved cleavage rate. …”
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Well-of-the-well (WOW) versus polyester mesh (PM): a comparison of single-embryo culture systems in bovines by Daniel Contreras-Benicio, Beatriz Elena Castro-Valenzuela, Juan Alberto Grado-Ahuir, María Eduviges Burrola-Barraza

“…Blastocysts in all three systems showed adequate amounts of ICM and apoptotic cells. …”
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Beneficial Effects of Catalpol Supplementation during In Vitro Maturation of Porcine Cumulus-Oocyte Complexes by Yanxin Wang, Ye Xu, Sijia Li, Xi Yan, Xiaofen Yang, Mengjia Chen, Yun Wang, Ruru Jia, Dongping Zhou, Deshun Shi, Fenghua Lu

“…It also increased oocyte glutathione (GSH), mitochondrial membrane potential and blastocyst cell number. However, DNA damage as well as reactive oxygen species (ROS) and malondialdehyde (MDA) levels. …”
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Effects of total gonadotropin dose on embryo quality and clinical outcomes with AMH stratification in IVF cycles: a retrospective analysis of 12,588 patients by Xiaoxue Shen, Yuhan Guo, Yan Liu, Wenyan Song, Gang Li, Haixia Jin

“…However, when women were 35–42 years old, regardless of AMH levels, the blastocyst formation rate and high-quality blastulation rate decreased as Gn dose increased. …”
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Performance of preimplantation genetic testing for aneuploidy in IVF cycles for patients with advanced maternal age, repeat implantation failure, and idiopathic recurrent miscarria... by Chun-I. Lee, Cheng-Hsuan Wu, Yi-Ping Pai, Yu-Jun Chang, Chung-I. Chen, Tsung-Hsien Lee, Maw-Sheng Lee

“…Vitrification was performed after blastocyst biopsy, and thawed euploid embryos were transferred in a nonstimulated cycle. …”
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Removal of cumulus cells around 20 h after the start of in vitro maturation improves the meiotic competence of porcine oocytes via reduction in cAMP and cGMP levels by Pilar FERRÉ-PUJOL, Xuan Khanh NGUYEN, Tomoki NAGAHARA, Thi Tra Mi BUI, Takuya WAKAI, Hiroaki FUNAHASHI

“…In conclusion, denuding oocytes at 20 h of IVM caused a significant reduction in ooplasmic cAMP and cGMP levels and increased the meiotic competence of the oocytes without any reduction in blastocyst formation, even in the case of SF-derived oocytes.…”
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mRNA Expression and Role of PPARγ and PPARδ in Bovine Preimplantation Embryos Depending on the Quality and Developmental Stage by Katarzyna Suwik, Emilia Sinderewicz, Dorota Boruszewska, Ilona Kowalczyk-Zięba, Joanna Staszkiewicz-Chodor, Krzysztof Łukaszuk, Izabela Wocławek-Potocka

“…In addition, the expression of PPARδ and PPARγ correlated with the expression of quality markers in bovine blastocysts. Positive correlations were stronger and more frequent in the group of early-cleaved embryos, whereas the negative correlations were typical for the group of late-cleaved embryos. …”
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#210 : The Comparison of Agonist Trigger and Dual Trigger in Our IVF Population - A Single Center Retrospective Study by Seema Pandey, Pratik Vaishnav, Akhileshwar Yadav

“…Conclusion: Dual trigger treatment with GnRH agonist and hCG is not inferior in terms of live birth rate, blastocyst, & M2 oocytes compared to GnRH agonist & hCG trigger apart from avoiding the danger of OHSS.…”
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Comparison of different methods for exogenous DNA uptake by bovine spermatozoa by Renata Simões, Weber Beringui Feitosa, Marcella Pecora Milazzotto, Alessandra Corallo Nicacio, Flavia Regina Oliveira de Barros, José Sergio de Arruda Gonçalves, Mariana Groke Marques, José Antônio Visintin, Mayra Elena Ortiz D'Ávila Assumpção

“…No effect of treatment was observed on cleavage, blastocyst and hatching rates. In addition, percentage of DNA positive blastocysts did not differ among experimental groups. …”
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