Subcutaneous progesterone administration provides a similar ongoing pregnancy rate compared with intramuscular progesterone administration in hormone replacement therapy frozen emb... by Fazilet K. Boynukalin, M.D., MSc., Remzi Abali, M.D., Meral Gultomruk, B.Sc., Berfu Demir, M.D., M.Sc., Zalihe Yarkiner, M.Sc., Ph.D., Guvenc Karlikaya, M.D., Mustafa Bahceci, M.D., Dominique de Ziegler, M.D.

“…In the first FET cycle of a freeze-all cycle using single blastocyst transfers, a woman aged ≤35 was included. …”
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Meiotic and developmental competence of growing pig oocytes derived from small antral follicles is enhanced in culture medium containing FGF2, LIF, and IGF1 (FLI medium) by Alexandra Rosenbaum Bartková, Lucie Němcová, Veronika Kinterová, Dominika Radová, František Strejček, Tereza Toralová, Jozef Laurinčík, Radek Procházka

“…Results The maturation and blastocyst rates of oocytes gained from LF were significantly higher than that from SF in the control medium. …”
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#169 : The Use of PIEZO-ICSI Increases Fertilisation and Lowers Degeneration Rates when Compared to Conventional ICSI: In a Singapore Setting by Wei Hou Lim, Wei Hou Lim, Pei Fang Yap, Heng Hao Tan, Colin Soon Soo Lee

“…Primary outcomes were fertilisation, degeneration, blastulation and blastocyst usable rates. Secondary outcomes include clinical pregnancy, implantation and miscarriage rates. …”
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Effect of Mechanical Micro-Vibrations on The Efficiency of Leopard Inter-Species Somatic Cell Nuclear Transfer by Maryam Shahverdi, Vahid Akbarinejad, Azam Dalman, Mostafa Hajinasrollah, Mehdi Vodjgani, Nima Tanhaei Vash, Mohammad Hossein Nasr-Esfahani, Poopak Eftekhari-Yazdi

“…The total number of blastocyst cells tended to be lower in the micro-vibration than in the control group (P=0.075). …”
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Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs by Zhi Yin, Jing-Tao Sun, Hong-Di Cui, Chao-Qian Jiang, Yu-Ting Zhang, Sanghoon Lee, Zhong-Hua Liu, Jun-Xue Jin

“…The results showed that 10 µg/mL TA significantly improved the cumulus expansion index (CEI), cumulus-expansion-related genes (<i>PTGS1</i>, <i>PTGS2</i>, <i>PTX-3</i>, <i>TNFAIP6</i> and <i>HAS2</i>) expression and blastocyst formation rates after parthenogenetic activation (PA), <i>in vitro</i> fertilization (IVF) and somatic cell nuclear transfer (SCNT) compared to the control groups, but not oocyte nuclear maturation. …”
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Influence of the number of washings for embryos on non-invasive preimplantation chromosome screening results by Xiaomei Kang, Meiting Wen, Jie Zheng, Fangxin Peng, Ni Zeng, Zhu Chen, Yanting Wu, Hong Sun

“…ObjectiveTo explore the effect of varying numbers of embryo washings prior to blastocyst formation in non-invasive preimplantation chromosome screening (NICS) on the accuracy of NICS results.MethodsIn this study, 68 blastocysts from preimplantation genetic testing (PGT)-assisted pregnancy were collected at our institution. …”
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Tannin Reduces the Incidence of Polyspermic Penetration in Porcine Oocytes by Jing-Tao Sun, Jia-Hui Liu, Xi-Qing Jiang, Xin Luo, Jin-Dong Yuan, Qi Zhang, Xin-Yue Qi, Sanghoon Lee, Zhong-Hua Liu, Jun-Xue Jin

“…In conclusion, TA during IVM enhances 2PN and blastocyst formation rates by regulating organelles’ functions and activities.…”
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Tight gene co-expression in BCB positive cattle oocytes and their surrounding cumulus cells by Bailey N. Walker, Jada Nix, Chace Wilson, Mackenzie A. Marrella, Savannah L. Speckhart, Lydia Wooldridge, Con-Ning Yen, Jocelyn S. Bodmer, Laila T. Kirkpatrick, Sarah E. Moorey, David E. Gerrard, Alan D. Ealy, Fernando H. Biase

“…Results The BCB positive oocytes were twice as likely to produce a blastocyst in vitro compared to BCB- oocytes (P < 0.01). …”
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Different Strategies of Preimplantation Genetic Testing for Aneuploidies in Women of Advanced Maternal Age: A Systematic Review and Meta-Analysis by Wei-Hui Shi, Zi-Ru Jiang, Zhi-Yang Zhou, Mu-Jin Ye, Ning-Xin Qin, He-Feng Huang, Song-Chang Chen, Chen-Ming Xu

“…For different stages of embryo biopsy, only the subgroup of blastocyst biopsy showed a higher live birth rate in women with PGT-A (RR = 1.36, 95% CI 1.04–1.79). …”
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Roles of transcription factor eomesodermin in the trophectoderm and definitive endoderm lineages of the murine embryo by Bisia, AM

“…Initially it is expressed in the trophectoderm (TE) of the pre-implantation blastocyst, where it is essential for peri-implantation development. …”

Transcriptome profiling of human pre-implantation development. by Pu Zhang, Marco Zucchelli, Sara Bruce, Fredwell Hambiliki, Anneli Stavreus-Evers, Lev Levkov, Heli Skottman, Erja Kerkelä, Juha Kere, Outi Hovatta

“…PRINCIPAL FINDINGS: We found that the preimplantation development consisted of two main transitions: from metaphase-II oocyte to 4-cell embryo where mainly the maternal genes were expressed, and from 8-cell embryo to blastocyst with down-regulation of the maternal genes and up-regulation of embryonic genes. …”
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Roles of cell differentiation factors in preimplantation development of domestic animals by Ken SAWAI

“…In mammalian embryos, the first visible differentiation event is the segregation of the inner cell mass (ICM) and trophectoderm (TE) during the transition from the morula to the blastocyst stage. The ICM, which is attached to the inside of the TE, develop into the fetus and extraembryonic tissues, while the TE, which is a single layer surrounding the fluid-filled cavity called the blastocoel, will provide extraembryonic structures such as the placenta. …”
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The Mesenchymal Stem Cells From The Veterinary Sciences Perspective by Nancy B. Riaño G., Victor J. Vera A., Luis Carlos Villamil J.

“…The origin of the stem cells can be embryonic (blastomers and cells of the internal mass of the blastocyst) or somatic (pluripotential cells from adult tissues). …”
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Developmental staging of Banf1 : early embryonic lethality in the mouse with doubly-disrupted gene expression by Ma, Hui Ling

“…In this study, BAF is shown to be expressed in all cells of E3.5 stage mouse blastocyst. We found BAF deficiency to cause impairment of inner cell mass growth and expansion in vitro, which is the likely cause of early embryonic lethality at peri-implantation stages. …”
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Moral uncertainty and the farming of human-pig chimeras by Koplin, J, Wilkinson, D

“…It may soon be possible to generate human organs inside of human-pig chimeras via a process called interspecies blastocyst complementation. This paper discusses what arguably the central ethical concern is raised by this potential source of transplantable organs: that farming human-pig chimeras for their organs risks perpetrating a serious moral wrong because the moral status of human-pig chimeras is uncertain, and potentially significant. …”

Stem cell medicine: umbilical cord blood and its stem cell potential. by Watt, S, Contreras, M

“…Stem cells may be sourced or derived from blood and tissues postnatally ('adult' stem cells), from the fetus (fetal stem cells) or from the blastocyst in the developing embryo prior to implantation (embryonic stem cells), each forming a unique component of the revolution in stem cell research and therapies. …”

#276 : Impact of Previous Cesarean Delivery on Pregnancy and Neonatal Outcomes of Subsequent In Vitro Fertilization/Intracytoplasmic Sperm Microinjection and Single Frozen-Thawed E... by Kexin Cao, Qian Zhang, Jie Li, Xinyu Liu, Yueyue Yan, Wei Zhou, Tianxiang Ni, Junhao Yan

“…Conclusion: Compared to VD, a previous CD is associated with a significant reduction in the rates of biochemical pregnancy, clinical pregnancy and live birth in patients undergoing subsequent single frozen-thawed blastocyst transfer.…”
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Function of JARID2 in bovines during early embryonic development by Yao Fu, Jia-Jun Xu, Xu-Lei Sun, Hao Jiang, Dong-Xu Han, Chang Liu, Yan Gao, Bao Yuan, Jia-Bao Zhang

“…The results showed that the relative expression levels of these genes in 2-cell embryos weresignificantly higher than those in the blastocyst stage, and expression levels were significantly increased after JARID2 knockdown. …”
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Growth hormone supplementation during ovarian stimulation in women with advanced maternal age undergoing preimplantation genetic testing for Aneuploidy by Yilun Sui, Min Xiao, Jing Fu, Lu Li, Yining Xu, Caixia Lei, Xiaoxi Sun

“…The GH group had a significantly higher euploid blastocyst rate per cohort (32.47% vs. 21.34%, P = 0.00) and mean euploid blastocyst rate per cycle (per biopsy cycle 0.35 ± 0.40 vs. 0.21 ± 0.33, P = 0.00; per OS cycle 0.27 ± 0.38 vs. 0.16 ± 0.30, P = 0.02). …”
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Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep by Alicia Martín-Maestro, Irene Sánchez-Ajofrín, Carolina Maside, Patricia Peris-Frau, Daniela-Alejandra Medina-Chávez, Beatriz Cardoso, José Carlos Navarro, María Rocío Fernández-Santos, José Julián Garde, Ana Josefa Soler

“…The percentage of cleavage, blastocyst rates and cumulus cell parameters (viability, active mitochondria and GSH/ROS ratio) were also decreased. …”
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