Warming Oocytes with a Universal Warming Medium and Protocol is as Good as the Company Media and Protocols by Penny CHEN, Trishla GOLECHHA, Greeshma GADDAM, Sharika PINNADUWA, Farjana YASMIN, Aarshiya, Nehal NAIK, Preethi SELVARAMANUJAM, Sanya KALRA, Sally CATT

“…Fertilization rates [S/S (43%), S/U (40%), V/V (62%), V/U (57%), M/M (59%), M/U (45%)] and blastocyst rates [S/S (61%), S/U (41%), V/V (60%), V/U (75%), M/M (54%), M/U (60%)] did not differ. …”
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Detection of endometrial and subendometrial vasculature on the day of embryo transfer and prediction of pregnancy during fresh in vitro fertilization cycles by Ari Kim, Hyuk Jung, Won June Choi, Sung Nam Hong, Heung Yeol Kim

“…Objectives: Successful implantation depends on interaction between a blastocyst and a receptive endometrium. Endometrial vasculature is important in the early endometrial response to blastocyst implantation, and vascular changes can affect uterine receptivity. …”
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Pregnancy outcome and follow-up of offspring of donor oocytes recipient from PCOS patients by Yan Jiang, Jing-Chuan Yuan, Ge Song, Xu-Hui Zhang, Sui-Bing Miao, Xiao-Hua Wu

“…Medical records were reviewed, and rates of fertilization, cleavage, high-quality embryos and blastocysts were compared between PCOS and non-PCOS groups. …”
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Pre-pregnancy obesity is not associated with poor outcomes in fresh transfer in vitro fertilization cycles: a retrospective study by Ping Tao, Xiaohong Yan, Yan Yao, Zhanxiang Wang, Youzhu Li

“…Available embryos rate (61.59% vs. 57.32%, p = 0.007) and blastocyst development rates (77.98% vs. 66.27%, p < 0.001) were higher in the obesity group compared to the normal BMI group. …”
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Novel Oocyte-Secreted Factors Improve Mouse IVM Outcomes by Dulama RICHANI, Yiqing ZHAO, Xiuhua LIAO, Jared M. CAMPBELL, Abbas HABIBALAHI, William A. STOCKER, Ewa M. GOLDYS, Craig A. HARRISON, Robert B. GILCHRIST

“…In bi-phasic IVM, cumulin did not significantly alter embryo yield (n=387-424 oocytes) or blastocyst cell number or allocation (n=84-112 blastocysts). …”
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Protodioscin protects porcine oocytes against H2O2-induced oxidative stress during in vitro maturation by So-Hee Kim, Seung-Eun Lee, Jae-Wook Yoon, Hyo-Jin Park, Seung-Hwan Oh, Do-Geon Lee, Da-Bin Pyeon, Eun-Young Kim, Se-Pill Park

“…The total cell number per blastocyst was significantly higher in the 10PD group than in the control group. …”
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LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene SIN3A by Daoyu Zhang, Yongfeng Zhou, Rong Huang, Yanhui Zhai, Di Wu, Xinglan An, Sheng Zhang, Lijing Shi, Qi Li, Xiangjie Kong, Hao Yu, Ziyi Li

“…The lncRNAs were highly expressed in the 4-cell to blastocyst stage but barely expressed in the oocytes and 2-cell stage. …”
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Effects of Regulating Hippo and Wnt on the Development and Fate Differentiation of Bovine Embryo by Peipei Zhang, Hang Zhang, Chongyang Li, Baigao Yang, Xiaoyi Feng, Jianhua Cao, Weihua Du, Muhammad Shahzad, Adnan Khan, Shao-Chen Sun, Xueming Zhao

“…We comprehensively analyzed the impact of LPA and DKK1 on various developmental parameters of the bovine embryo, such as blastocyst formation, differential cell counts, YAP fluorescence intensity and apoptosis rate. …”
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Experimental Evidence Reveals Both Cross-Infection and Cross-Contamination Risk of Embryo Storage in Liquid Nitrogen Biobanks by Clara Marin, Ximo Garcia-Dominguez, Laura Montoro-Dasi, Laura Lorenzo-Rebenaque, José S. Vicente, Francisco Marco-Jimenez

“…In vivo produced late morulae–early blastocyst stages (72 h) embryos were used (n = 480). …”
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Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro by Annelies Raes, Eline Wydooghe, Krishna Chaitanya Pavani, Osvaldo Bogado Pascottini, Katleen Van Steendam, Maarten Dhaenens, Annekatrien Boel, Sonia Heras, Björn Heindryckx, Luc Peelman, Dieter Deforce, Filip Van Nieuwerburgh, Geert Opsomer, Ann Van Soom, Katrien Smits

“…While human in vitro embryo production is generally performed individually, animal models have shown that culturing embryos in groups improves blastocyst yield and quality. Paracrine embryotrophins could be responsible for this improved embryo development, but their identity remains largely unknown. …”
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Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro by S. Hua, J. Lan, Y.G. Liu, Y.L. Song, J. Liu, Y.S. Wang, T. Zhang, Y. Zhang

“…The expression levels of 4 genes (Na/K-ATPase, Glut-1, ActR II and Smad2) in blastocysts obtained in experiment 2 were higher than those obtained in experiment 1. …”
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The Human Early Maternal–Embryonic Interactome by Adam Stevens, Taqua Khashkhusha, Megan Sharps, Terence Garner, Peter T. Ruane, John D. Aplin

“…Methods: Bulk and single cell RNA-sequencing of endometrial epithelium and scRNAseq of day 6 and 7 trophectoderm (TE) were used to model the initial encounter between the blastocyst and the maternal uterine lining epithelium in silico. …”
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Production of transgenic first filial puppies expressing mutated human amyloid precursor protein gene by Mohammad Shamim Hossein, Young-Bum Son, Young-Bum Son, Yeon Woo Jeong, Yeon Woo Jeong, Yeon Ik Jeong, Mi Na Kang, Eun Ji Choi, Kang Bae Park, Yu Ra Bae, Dae Young Kim, Woo Suk Hwang, Woo Suk Hwang

“…In Experiment II, a total of 59, 70, and 65 presumptive zygotes produced by fresh, Percoll gradient, and swim-up methods, respectively, were transferred to surrogates (5 for each group); the Percoll gradient (27.27%) and swim-up samples (14.29%) showed the highest blastocyst formation rates, while fresh diluted semen did not produce any blastocyst. …”
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Identification and Functional Analysis of Transcriptome Profiles, Long Non-Coding RNAs, Single-Nucleotide Polymorphisms, and Alternative Splicing from the Oocyte to the Preimplanta... by Zijing Zhang, Qiaoting Shi, Xiaoting Zhu, Lei Jin, Limin Lang, Shijie Lyu, Xiaoling Xin, Yongzhen Huang, Xiang Yu, Zhiming Li, Sujuan Chen, Zhaoxue Xu, Wei Zhang, Eryao Wang

“…We used various methods to construct a profile to characterize cellular and molecular features and systematically analyze the related GO and KEGG profile of cells of all stages from the oocyte to the blastocyst. This large-scale, single-cell atlas provides key cellular information and will likely assist clinical studies in improving preimplantation genetic diagnosis.…”
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Intravenous immunoglobulin for patients with unexplained recurrent implantation failure: a 6-year single center retrospective review of clinical outcomes by Einav Kadour Peero, Shorooq Banjar, Rabea Khoudja, Shaonie Ton-leclerc, Coralie Beauchamp, Joanne Benoit, Marc Beltempo, Michael H. Dahan, Phil Gold, Isaac Jacques Kadoch, Wael Jamal, Carl Laskin, Neal Mahutte, Simon Phillips, Camille Sylvestre, Shauna Reinblatt, Bruce D. Mazer, William Buckett, Genevieve Genest

“…IVIg can be considered in well selected patients with $$\ge$$ ≥ 5 previous unexplained, high quality blastocyst transfer failures. A randomized controlled trial is needed to confirm these findings.…”
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An investigation of the effects of laser-assisted zona pellucida drilling on the preimplantation mouse embryo and the competency of embryo implantation by Coward, K, Jones, C, Liu, Y

“…</p> <p><strong>Main Outcome Measures:</strong> Blastocyst formation, hatching status, and hatching morphology at E4.5. …”

In Utero Programming of Testicular Cancer by David Elad, Ariel J. Jaffa, Dan Grisaru, Ilan Leibovitch

“…Moreover, precursors for TGCC may originate as early as during fertilization or implantation of the blastocyst. Further investigations of human developmental biology, both in vivo and in vitro, are needed in order to establish better understanding of in utero programming of future wellbeing or diseases.…”
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Impact of gamete health on fertilization and embryo development: An overview by Jaypalsinh B Chorya, Tarunkumar V Sutaria, Ravjibhai K Chaudhari, Chandrakant F Chaudhari

“…The abnormal spermatozoa adversely affect fertilization events, which results in reduced cleavage/blastocyst/ implantation and pregnancy rate during assisted reproductive techniques. …”
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Human embryos in a dish – modeling early embryonic development with pluripotent stem cells by Xiukun Wang, Guang Hu

“…Furthermore, the formation of these lineages followed the same sequence and pace of blastocyst development, and was also dependent on the same pathways required for lineage specification. …”
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First successful pregnancy in Switzerland after prospective sex determination of the embryo through the separation of X-chromosome bearing spermatozoa by Christian De Geyter, Oliver Sterthaus, Peter Miny, Friedel Wenzel, Olav Lapaire, Maria De Geyter, Gideon Sartorius

“…RESULTS: After transfer of one single blastocyst, an intrauterine pregnancy arose. In the ninth week of gestation, the female sex of the embryo was confirmed with proof of absence of the SRY gene of the Y-chromosome. …”
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