A case report of Uterine-ovarian pregnancy after 13 years by T Naderi, H Zeinali

“…Naturaly blastocyte implants in the uterine cavity.implantation of blastocyst in anyplace other than the endometrial lining of the uterine cavity is considered an ectopic pregnancy.more than 95% of ectopic pregnancies are tubal pregnancies.ovarian ectopic pregnancy is a rare case with an estimated incidence ranging from % 0.05-1 of the extra uterine pregnancies.if one blastocytes implant simultaneously in the uterine cavity and the other anywhere else other than uterine cavity it is referred to as heterotype pregnancy.this is a rare type of pregnancy and depends on implantion place of the second blastocyte and is named uterine-ovarian,tubal-uterine or...... pregnancy.this is a case report of uterine-ovarian pregnancy in which a live child was born about 13 years ago however a calcified and a term twin fetus remaining in the ovary was taken out with laparotomy.…”
Get full text

Lipid droplets are formed in 2-cell-like cells by Asuka FURUTA, Toshinobu NAKAMURA

“…Embryonic stem (ES) cells, derived from the inner cell mass of a blastocyst, are believed to pluripotent cells and give rise to embryonic, but not extraembryonic, tissues. …”
Get full text

Transplanting stem cells: potential targets for immune attack. Modulating the immune response against embryonic stem cell transplantation. by Boyd, A, Higashi, Y, Wood, K

“…Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of a pre-implantation blastocyst that can produce all cells and tissues of the foetus. …”

The transcription factor NF-ATc1 regulates lymphocyte proliferation and Th2 cytokine production. by Yoshida, H, Nishina, H, Takimoto, H, Marengère, L, Wakeham, A, Bouchard, D, Kong, Y, Ohteki, T, Shahinian, A, Bachmann, M, Ohashi, P, Penninger, J, Crabtree, G, Mak, T

“…The role of NF-ATc1 was investigated in recombination activating gene-1 (RAG-1)-deficient blastocyst complementation assays using homozygous NF-ATc1-/- mutant ES cell lines. …”

PLCζ: A sperm-specific trigger of Ca2+ oscillations in eggs and embryo development by Saunders, C, Larman, MG, Parrington, J, Cox, L, Royse, J, Blayney, L, Swann, K, Lai, F

“…Moreover, the PLCζ content of a single sperm was sufficient to produce Ca2+ oscillations as well as normal embryo development to blastocyst. Our results are consistent with sperm PLCζ as the molecular trigger for development of a fertilised egg into an embryo.…”

PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development. by Saunders, C, Larman, MG, Parrington, J, Cox, L, Royse, J, Blayney, L, Swann, K, Lai, F

“…Moreover, the PLC zeta content of a single sperm was sufficient to produce Ca(2+) oscillations as well as normal embryo development to blastocyst. Our results are consistent with sperm PLC zeta as the molecular trigger for development of a fertilised egg into an embryo.…”

Pontin is essential for murine hematopoietic stem cell survival. by Bereshchenko, O, Mancini, E, Luciani, L, Gambardella, A, Riccardi, C, Nerlov, C

“…Through germline inactivation of Ruvbl1, the gene encoding Pontin, we found it to be essential for early embryogenesis, as Ruvbl1 null embryos could not be recovered beyond the blastocyst stage where proliferation of the pluripotent inner cell mass was impaired. …”

Evaluate the developmental competence of human 8-cell embryos by single-cell RNA sequencing by Weizhou Wang, Mengmeng Zhao, Haiyang Zuo, Jingyao Zhang, Bin Liu, Fu Chen, Pengyun Ji, Guoshi Liu, Shuai Gao, Wei Shang, Lu Zhang

“…The total number of genes detected in 8-cell embryos that failed to form blastocyst including maternal and zygotic mRNAs was reduced. …”
Get full text

Association between duration of progesterone supplementation and clinical outcomes in artificial frozen-thawed embryo transfer cycles by Ling Liu, Hongyan Zhou, Jie Hu, Xingyu Sun, Doudou Liu, Guiying Huang

“…The duration of progesterone administration (mean: 5.6 ± 0.5 days; range:5–6 days) before frozen blastocyst transfer may not affect clinical pregnancy (OR 1.339; 95% CI, 0.717–2.497).ConclusionThere may be no significant correlation between the duration of progesterone supplementation and pregnancy outcomes in artificial FET cycles, although the clinical pregnancy rate was higher when progesterone supplementation was extended for one day before FET.…”
Get full text

MicroRNAs from Extracellular Vesicles Secreted by Bovine Embryos as Early Biomarkers of Developmental Competence by Bárbara Melo-Baez, Yat S. Wong, Constanza J. Aguilera, Joel Cabezas, Ana C. F. Mançanares, Gonzalo Riadi, Fidel O. Castro, Lleretny Rodriguez-Alvarez

“…Embryos that reach the blastocyst stage secrete fewer and smaller vesicles. …”
Get full text

Establishment of linkage phase, using Oxford Nanopore Technologies, for preimplantation genetic testing of Coffin-Lowry syndrome with a de novo RPS6KA3 mutation by Xiaojun Wen, Jing Du, Zhiming Li, Nengqing Liu, Junye Huo, Jieliang Li, Wanna Ke, Jiaqi Wu, Xiaowu Fang, Xiufeng Lin, Xiufeng Lin

“…The results of PGT showed that three of the eight blastocysts were normal, without the DNM. Moreover, the patient had a successful pregnancy, after transfer of a normal blastocyst into the uterus, and delivered a healthy baby.Conclusion: The ONT platform, combined with the MARSALA method, can be used to perform PGT for DNM patients without the need for other samples as a reference.…”
Get full text

The apoptotic inhibitor z-DEVD-fmk improves the viability and maturation rates of Iberian red deer oocytes while reducing apoptotic markers by Daniela Alejandra Medina-Chávez, Juan Angel Laborda, Olga García-Álvarez, Jose Antonio Ortiz, Carmen María Picazo, Alejandro Maroto-Morales, María Rocío Fernández-Santos, J. Julián Garde, Ana Josefa Soler, Irene Sánchez-Ajofrín

“…Nevertheless, the blastocyst production was not different between both experimental groups (inhibitor: 7.35 ± 2.30 vs. control: 13.77 ± 2.30). …”
Get full text

Autologous Platelet-Rich Plasma Improves Pregnancy Outcomes of Patients with Thin Endometrium Regardless Endometrial Thickening: Multicenter Retrospective Study with Elimination of... by Satoshi Suzuki, Maki Kusumi, Tomoko Maeda, Kiyotaka Kawai, Toshihiro Kawamura, Eri Okamoto, Toushun Jo, Ryo Tsutsumi, Satoru Takamizawa, Takahiro Nakayama, Motowo Nabeta, Yukio Nishiyama, Mari Nomiyama, Kenji Furui, Yoshiharu Morimoto, Osamu Tsutsumi

“…A total of 208 patients who underwent single blastocyst transfer (SBT) after PRP due to thin endometrium (¡8 mm) in their previous cycle were included in the study. …”
Get full text

Impact of the addition of Early Embryo Viability Assessment to morphological evaluation on the accuracy of embryo selection on day 3 or day 5: a retrospective analysis by Alberto Revelli, Stefano Canosa, Andrea Carosso, Claudia Filippini, Carlotta Paschero, Gianluca Gennarelli, Luisa Delle Piane, Chiara Benedetto

“…Four groups were considered: (a) patients receiving day 3 DET with embryos selected by standard morphology (DET-3 M, n = 106, receiving 212 embryos), (b) patients receiving day 3 DET with embryos selected by morphology plus Eeva™ (DET-3 ME group, n = 48, receiving 96 embryos), (c) patients receiving day 5 SET with a blastocyst selected by standard morphology (SET-5 M group, n = 126, receiving 126 embryos), and (d) patients receiving day 5 SET with a blastocyst selected by morphology plus Eeva™ (SET-5 ME group, n = 48, receiving 48 embryos). …”
Get full text

Chrysoeriol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress and Autophagy by Chao-Rui Wang, He-Wei Ji, Sheng-Yan He, Rong-Ping Liu, Xin-Qin Wang, Jing Wang, Chu-Man Huang, Yong-Nan Xu, Ying-Hua Li, Nam-Hyung Kim

“…The results showed that supplementation with 1 µM CHE significantly increased the blastocyst rate and total cell number of embryos in vitro. …”
Get full text

The number of primitive endoderm cells in the inner cell mass is regulated by platelet-derived growth factor signaling in porcine preimplantation embryos by Jong-Nam Oh, Mingyun Lee, Gyung Cheol Choe, Dong-Kyung Lee, Kwang-Hwan Choi, Seung-Hun Kim, Jinsol Jeong, Chang-Kyu Lee

“…Embryos in the early blastocyst stage possess two different cell populations, the inner cell mass (ICM) and trophectoderm. …”
Get full text

Increasing dominant follicular proportion negatively associated with good clinical outcomes in normal ovarian responders using the depot GnRH agonist protocol: a large-sample retro... by Houming Su, Youhua Lai, Jie Li, Tingting Liao, Licheng Ji, Xinyao Hu, Kun Qian

“…The binary logistic regression and multivariate linear regression were used to assess whether the DFP was associated with clinical pregnancy, the number of frozen blastocysts, the blastocyst formation rate, and the low number of frozen blastocysts. …”
Get full text

#81 : Result of Non-Invasive Preimplantation Genetic Testing for Aneuploidy in Different Culture Medium Condition by Pritta Ameilia Iffanolida, Achmad Kemal Harzif, Kresna Mutia, Ririn Rahmala Febri, Deka Putri Gunarwati, Mila Maidarti, Budi Wiweko, Andon Hestiantoro

“…In day 5/6 we performed TE biopsy, and the embryo was freezing, then we took the spent blastocyst medium (SBM). A total 240 samples of SCM, TCM and TE were screened for chromosomal abnormality by Next Generation Sequencing (NGS) method. …”
Get full text

#245 : The Clinical Outcome of Different Embryo Transfer Strategies After Late Rescue ICSI Procedure by Rui Yang, Tian Tian, Xiaxuan Zhu, Rong Li, Ping Liu, Jie Qiao

“…Background and Aims: To evaluate the safety profile and effectiveness of freeze-all blastocyst transfer when applied in the late rescue intracytoplasmic sperm injection (r-ICSI). …”
Get full text

Effect of Triclosan Exposure on Developmental Competence in Parthenogenetic Porcine Embryo during Preimplantation by Min Ju Kim, Hyo-Jin Park, Sanghoon Lee, Hyo-Gu Kang, Pil-Soo Jeong, Soo Hyun Park, Young-Ho Park, Jong-Hee Lee, Kyung Seob Lim, Seung Hwan Lee, Bo-Woong Sim, Sun-Uk Kim, Seong-Keun Cho, Deog-Bon Koo, Bong-Seok Song

“…Developmental parameters including cleavage and blastocyst formation rates, developmental kinetics, and the number of blastomeres were assessed. …”
Get full text