Improving Cryopreservation Efficiency and Pregnancy Rate through Superovulation with Follicle-Stimulating Hormone in Korean Hanwoo Cows via Ovum Pick Up by Daehyun Kim, Junkoo Yi

“…Moreover, the FSH-treated group achieved a notably higher blastocyst rate (44.7%) compared to the control group (31.1%) (<i>p</i> < 0.05). …”
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The p66(Shc) adaptor protein controls oxidative stress response in early bovine embryos. by Dean H Betts, Nathan T Bain, Pavneesh Madan

“…Using RNA interference in bovine zygotes we show that p66Shc knockdown embryos exhibited increased MnSOD levels, reduced intracellular ROS and DNA damage that resulted in a greater propensity for development to the blastocyst stage. P66Shc knockdown embryos were stress resistant exhibiting significantly reduced intracellular ROS levels, DNA damage, permanent 2-4 cell embryo arrest and diminished apoptosis frequencies after oxidant treatment. …”
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Cdc20 is critical for meiosis I and fertility of female mice. by Fang Jin, Masakazu Hamada, Liviu Malureanu, Karthik B Jeganathan, Wei Zhou, Dean E Morbeck, Jan M van Deursen

“…When mated with wild-type males, hypomorphic females yield nearly normal numbers of fertilized eggs, but as these embryos develop, they become malformed and rarely reach the blastocyst stage. In exploring the underlying mechanism, we uncover that the vast majority of these embryos have abnormal chromosome numbers, primarily due to chromosome lagging and chromosome misalignment during meiosis I in the oocyte. …”
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EMERGENCY CONTRACEPTION by Dragana Pantić, Jasmina Popović, Milena Veljković

“…Copper intrauterine device causes a foreign-body effect on the endometrium and a direct toxic effect to sperm and blastocyst.The Yuzpe regimen reduces the risk of pregnancy after a single act of sexual intercourse by about 75% and the levonorgestrel alone by about 85%. …”
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The Impact of Hypoxia in Early Pregnancy on Placental Cells by Hui Zhao, Ronald J. Wong, David K. Stevenson

“…This hypoxic phase overlaps with the most critical stages of placental development, i.e., blastocyst implantation, cytotrophoblast invasion, and spiral artery remodeling initiation. …”
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RESEARCHES REGARDING THE INFLUENCE OF THE NUMBER OF CUMULAR CELLS LAYER OVER THE OOCYTE MATURATION EFFICIENCY by V. CARABĂ, I. VINTILĂ, ALEXANDRA IVAN, ADA TELEA, D. MĂNDIŢĂ

“…We select the oocytecumulus complexes collected both from cows and sows according to the number of cumular cell layers and we watched their development to the blastocyst stade. Thus, we achieved three groups of COC (oocyte-cumulus complexes). …”
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The Effects of an In Vitro Oocyte Maturation System and Chlorogenic Acid Supplementation during Embryo Culture on the Development of Porcine Cloned Embryos Derived from Native Viet... by Thanh Van Nguyen, Lanh Thi Kim Do, Ngoc-Anh Thi Nguyen, Kazuhiro Kikuchi, Tamas Somfai, Takeshige Otoi

“…CGA supplementation to the culture medium significantly increased blastocyst formation rates in parthenogenetic embryos but not in SCNT embryos. …”
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Investigation of the effects of storage period for frozen bull semen on in vitro embryo production by Numan AKYOL, Talha Burak ERTEM, Ömer VARIŞLI

“…The cleavage ratio in vintage spermatozoa was found to be lower than in the control group (P<0.02), the blastocyst ratio was also lower than in the control group (P<0.01). …”
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#93 : Analysis of 126 Uterine Adenomyosis Patients in 360 Cycles of Art Treatment Over 12 Years and Future Prospects by Atsushi Tanaka, Daisuke Nakajima, Motoi Nagayoshi, Phar.B. Izumi Tanaka, Takashi Yamaguchi

“…Results: There were no significant differences in the fertilization rate [70.3%(1316/1872), 69.8%(15570/22309)], cleavage rate [68.9%(1289/1872), 68.5%(15274/22309)] and blastocyst formation rate [42.6%(560/1316), 43.0%(6693/15570)] between the two groups. …”
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Assisted reproductive technologies in rhesus macaques by Wolf Don P

“…ICSI produced embryos have also served as the source of blastocysts for the derivation of embryonic stem cells. …”
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A protocol to study three-dimensional genome structure in individual mutant preimplantation mouse embryos by Daniel Gimenez-Llorente, Marta Portela, Ana Losada, Miguel Manzanares, Maria Jose Andreu

“…We developed single-blastocyst Hi-C in mutant mouse embryos to genotype them on sequence. …”
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Lipopolysaccharide-induced mechanisms of ovarian dysfunction in cows with uterine inflammatory diseases by Fumie MAGATA

“…Moreover, oocytes matured using LPS treatment are less likely to develop into the blastocyst stage. Such oocytes also have a reduced number of trophoblast cells in blastocysts. …”
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Xanthan gum and Locust bean gum gel supports in vitro development of porcine oocytes derived from early antral follicles by Yasuhisa MUNAKATA, Ayaka SUGIMOTO, Koumei SHIRASUNA, Takehito KUWAYAMA, Hisataka IWATA

“…Development to the blastocyst stage was 13.8% for the XG-LBG gels and 9.4% for PAG. …”
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Establishing an in vitro production program for buffalo embryos (Bubalus bubalis) in Colombia by Felipe Gamarra P., Viviana Rendón V., Aldemar Chávez R., Leonardo Perez S., Walter Cardona-Maya, Jesús Berdugo G.

“…Of the 783 oocytes, 92% were classified as viable (721/783) and were fertilized. The cleavage and blastocyst rate were 23% and 19% respectively. 37 embryos were transferred and 11 pregnancies were obtained, confirmed by rectal palpation 60 days after transfer, with a pregnancy rate of 29.7%. …”
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Single-cell multiomics analyses of spindle-transferred human embryos suggest a mostly normal embryonic development by Shuyue Qi, Wei Wang, Xiaohui Xue, Zhuo Lu, Jia Yan, Yunfei Li, Yu Zhang, Mingming Shu, Chunlan Song, Qihang Wang, Yunhai Chuai, Xinyu Zhai, Shujie Han, Fuchou Tang, Wei Shang

“…However, the DNA demethylation process in TE cells of ST blastocysts was slightly slower than that in the control blastocysts. …”
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Effect of lactation on oocyte quality and in vitro embryo production of Bos indicus cows by H. J. Narváez, D. Villalba, D. A. Vega

“…The results indicated that in lactating Gyr cows had the following values of studied variables: number of follicles visualised (22.1±11.38), number of recovered oocytes (17.4±10.21), number of grade III oocytes (7.0±5.86), rate of viable oocytes (72.1±13.11%), number of blastocysts D7 (2.5±2.26) and blastocyst rate (18.3±15.61%), in relation to non-lactating cows (27.7±11.53; 21.1±10.06; 9.8±6.08, 77.4±10.08%; 4.9±3.34; 30.2±15.41%, respectively). …”

Establishment of African pygmy mouse induced pluripotent stem cells using defined doxycycline inducible transcription factors by Sumito Matsuya, Kaoru Fujino, Hiroyuki Imai, Ken Takeshi Kusakabe, Wataru Fujii, Kiyoshi Kano

“…These findings suggest these iPSCs have potential utility in various analytical applications, including methods for blastocyst completion.…”
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TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes. by Mahdi Mahdipour, Ana Rita Canhoto Leitoguinho, Ricardo A Zacarias Silva, Helena T A van Tol, Tom A E Stout, Gabriela Rodrigues, Bernard A J Roelen

“…TACC3 mRNA was detected in bovine oocytes during meiosis using qRT-PCR, and while it was also expressed in cleavage stage embryos, its expression was down-regulated at the morula and blastocyst stages. Immunofluorescence was used to demonstrate that TACC3 co-localized with tubulin in the metaphase I and II spindles. …”
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#384 : Effectiveness of Progesterone Monitoring and Rescue Protocol in Frozen Embryo Transfer Cycle by Chien Nguyen, Ly Nguyen, Xuyen Nguyen

“…Results: In 2022, in Vinmec IVF center, there were in total 659 FET cycles, all was single blastocyst transfer and applied the mention protocol to rescue the cycle in case P4 level was less than 10 ng/ml. …”
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Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system. by Giuliana de Avila Ferronato, Carolina Mônica Dos Santos, Paola Maria da S Rosa, Alessandra Bridi, Felipe Perecin, Flávio Vieira Meirelles, Juliano Rodrigues Sangalli, Juliano Coelho da Silveira

“…There was a decrease in blastocyst rate and cell counting, a down-regulation of miR-615 expression, and an increase in the DNA global methylation and hydroxymethylation in embryos of LM group. …”
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