Dynamic Transcription of Distinct Classes of Endogenous Retroviral Elements Marks Specific Populations of Early Human Embryonic Cells by Göke, Jonathan, Lu, Xinyi, Chan, Yun-Shen, Ng, Huck-Hui, Ly, Lam-Ha, Sachs, Friedrich, Szczerbinska, Iwona

“…Conversion of human embryonic stem cells (hESCs) to an epiblast-like state activates blastocyst-specific ERV elements, indicating that their activity dynamically reacts to changes in regulatory networks. …”
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The role of declining ataxia-telangiectasia-mutated (ATM) function in oocyte aging by Suzuki, R, Tan, X, Szymanska, KJ, Kubikova, N, Perez, CA, Wells, D, Oktay, KH

“…After ATM knockdown, all embryos were of poor quality, and none reached the blastocyst stage. ATM knockdown was also associated with an increased aneuploidy rate compared to controls. …”

Fast-evolving homeobox genes in mammalian preimplantation development by Lewin, TD

“…Ectopic expression experiments of ETCHbox proteins in cell culture suggest them to be multifunctional regulators of several key processes during preimplantation development, including blastocyst formation; inner cell mass development; the activity of transposable elements; and cellular potency. …”

Interspecific variation in one-carbon metabolism within the ovarian follicle, oocyte, and preimplantation embryo: Consequences for epigenetic programming of DNA methylation by Clare, CE, Pestinger, V, Kwong, WY, Tutt, DAR, Xu, J, Byrne, HM, Barrett, DA, Emes, RD, Sinclair, KD

“…Here we report that transcripts for all bar two genes (i.e., BHMT, MAT1A) encoding enzymes in the linked methionine-folate cycles are expressed in all cell types within the ovarian follicle, oocyte, and blastocyst in the cow, sheep, and pig; as well as in rat granulosa cells (GCs) and human KGN cells (a granulosa-like tumor cell line). …”

Embryo culture conditions are significantly improved during uninterrupted incubation: A randomized controlled trial by Alhelou, Yousef, Mat Adenan, Noor Azmi, Ali, Jaffar

“…The fertilisation rate, development of day 2 and clinical pregnancy rates were similar in both treatments but the 8-cell development rate in all age groups combined (p = 0.016), blastocyst development rate (p = 0.0022) and the implantation rate (p = 0.0022) was significantly higher for the uninterrupted culture. …”

Effect of endomentrial rceptivity on in vitro fertilization/intracytoplasmic sperm injection outcome in HUSM by Mohamadzon, Erinna

“…The developed embryos were transferred into the uterus at blastocyst stage. Serum beta hCG was measured 14 days after embryo transfer. …”
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Impact of media supplements FGF2, LIF and IGF1 on the genome activity of porcine embryos produced in vitro by Alexandra Rosenbaum Bartkova, Lucie Nemcova, Frantisek Strejcek, Ahmed Gad, Veronika Kinterova, Martin Morovic, Michal Benc, Radek Prochazka, Jozef Laurincik

“…FLI medium resulted in a significantly higher rate of oocyte blastocyst maturation and formation compared to the control DMEM medium. …”
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Machine Learning Approach to Predict Clinical Pregnancy Potential in Women Undergoing IVF Program by Nining Handayani, Claudio Michael Louis, Alva Erwin, Tri Aprilliana, Arie A Polim, Batara Sirait, Arief Boediono, Ivan Sini

“…All patients underwent fresh embryo transfer at either the cleavage or blastocyst stage between January 2015 and December 2019. …”
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Analysis of clinical outcomes and meiotic segregation modes following preimplantation genetic testing for structural rearrangements using aCGH/NGS in couples with balanced chromoso... by Tatsuya Nakano, Michiko Ammae, Manabu Satoh, Satoshi Mizuno, Yoshiharu Nakaoka, Yoshiharu Morimoto

“…Results The genetically transferable blastocyst rate was 21.2% (51/241). Thirty five genetically transferable blastocysts were transferred into the uterine cavity. …”
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Isolation and characterization of mesenchymal stem cells derived from bovine Wharton's jelly and their potential for use in cloning by nuclear transfer by Carolina Gonzales da Silva, Carlos Frederico Martins, Tereza Cristina Cardoso, Elisa Ribeiro da Cunha, Heidi Christina Bessler, Concepta Margaret McManus, Ivo Pivato, Sônia Nair Báo

“…After classification, the WJCs were used in NT. Blastocyst formation rate by NT with WJCs at day 7 was 25.80±0.03%, similar to blatocyst rate with NT using skin fibroblasts (19.00±0.07%). …”
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Evidence For Hmgn2 Involvement in Mouse Embryo Implantation and Decidualization by Dang-Dang Li, Liang Yue, Zhan-Qing Yang, Lian-Wen Zheng, Bin Guo

“…Results: Hmgn2 was primarily accumulated in uterine luminal epithelia on day 4 of pregnancy and subluminal stromal cells around the implanting blastocyst at implantation sites on day 5. Similar results were observed during delayed implantation and activation. …”
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Effect of Magnetized Freezing Extender on Membrane Damages, Motility, and Fertility of Boar Sperm Following Cryopreservation by Seunghyung Lee, Yong-Min Kim, Hee-Tae Cheong, Choon-Keun Park, Sang-Hee Lee

“…The number of blastocysts was significantly higher in the 4000 G group than in the 0 G group. …”
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Role of Endometrial Extracellular Vesicles in Mediating Cell-to-Cell Communication in the Uterus: A Review by Jacob R. Beal, Qiuyan Ma, Indrani C. Bagchi, Milan K. Bagchi

“…These events include blastocyst implantation, uterine decidualization, uterine neoangiogenesis, differentiation of trophoblast stem cells into different trophoblast cell lineages, and formation of a placenta. …”
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Case report: A reciprocal translocation-free and pathogenic DUOX2 mutation-free embryo selected by complicated preimplantation genetic testing resulted in a healthy live birth by Biwei Shi, Yinghui Ye

“…A blastocyst lacking copy number variants and RecT but carrying the paternal gene mutation in DUOX2, c.2654G>T (p.R885L) was used for embryo transfer, resulting in a healthy female infant whose genetic properties were confirmed by amniocentesis. …”
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L-carnitine prevents bovine oocyte aging and promotes subsequent embryonic development by Wen-Jie JIANG, Xue-Rui YAO, Yu-Han ZHAO, Qing-Shan GAO, Qing-Guo JIN, Ying-Hua LI, ang-Guo YAN, Yong-Nan XU

“…After in vitro fertilization, the blastocyst formation rate in the aged oocytes post-LC treatment significantly increased compared to that in untreated aged oocytes (29.23 ± 2.20% vs. 20.90 ± 3.05%). …”
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Molecular Mechanisms Underlying Pluripotency and Self-Renewal of Embryonic Stem Cells by Fahimeh Varzideh, Jessica Gambardella, Urna Kansakar, Stanislovas S. Jankauskas, Gaetano Santulli

“…Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of the blastocyst. ESCs have two distinctive properties: ability to proliferate indefinitely, a feature referred as “self-renewal”, and to differentiate into different cell types, a peculiar characteristic known as “pluripotency”. …”
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Characterisation of the dynamic behaviour of lipid droplets in the early mouse embryo using adaptive harmonic generation microscopy by Wilson Tony, Debarre Delphine, Grieve Kate, Jesacher Alexander, Thayil Anisha, Watanabe Tomoko, Booth Martin, Srinivas Shankar

“…At fertilization, LD are small and uniformly distributed, but in the implanting blastocyst, LD are larger and enriched in the invading giant cells of the trophectoderm. …”
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Application of reproductive technologies to the improvement of dairy cattle genomic selection by N. S. Yudin, K. I. Lukyanov, M. I. Voevoda, N. A. Kolchanov

“…Successful methods for embryo genotyping for numerous markers after biopsy at the morula or blastocyst stage are based on whole genome amplification of embryo DNA. …”
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#38 : Maternal Age Affects Morula Compaction and Cell Polarity of Human Embryos by Kenji Ezoe, Tetsuya Miki, Keiichi Kato

“…In women of AMA, the blastocyst formation rate has been reported to decline due to an increase in embryo arrest at the morula stage. …”
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Cost-Effective Trap qPCR Approach to Evaluate Telomerase Activity: an Important Tool for Aging, Cancer, and Chronic Disease Research by Thalyta Nery Carvalho Pinto, Juliana Ruiz Fernandes, Liã Barbara Arruda, Alberto José da Silva Duarte, Gil Benard

“…Telomerase activity is observed during early embryonic development, but after the blastocyst stage, the expression of telomerase reduces. …”
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