Effect of Glycosaminoglycans on Fertilizing Ability and Developmental Potential of Bovine Embryos by Eun Young Kim, Eun Hyung Noh, Eun Ji Noh, Min Jee Park, Hyo Young Park, Dong Sun Lee, Key Zung Riu, Se Pill Park

“…Exposure to these GAGs also enhanced in vitro embryo development rates and embryo quality, and increased the ICM and total blastocyst cell numbers at d 8 after IVF (p<0.05). A real-time PCR analysis showed that the expression levels of pluripotency (Oct 4), cell growth (Glut 5), and anti-apoptosis (Bax inhibitor) genes were significantly higher in embryos derived from HA- or HP-treated sperm than in control or other treatment groups, while pro-apoptotic gene expression (caspase-3) was significantly lower in all GAG treatment groups (p<0.05). …”
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Melatonin’s effect on hair follicles in a goat (Capra hircus) animal model by Youjun Rong, Rong Ma, Yanjun Zhang, Yanjun Zhang, Zhenhua Guo

“…A network meta-analysis used data from oocyte development to blastocyst. The hair follicle genes regulated by melatonin performed KEGG and PPI. …”
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Optimized heterologous in vitro fertilization with Iberian ibex sperm and domestic goat oocytes by Nuria Martínez de los Reyes, Melissa Carvajal-Serna, Inés Flores-Borobia, Pilar Marigorta, Patricia Peris-Frau, Julián Santiago-Moreno, Pablo Bermejo-Álvarez, Priscila Ramos-Ibeas

“…We found that TALP medium provides optimal conditions to conduct heterologous ibex-goat IVF, yielding blastocyst rates above 50%, and that supplementation with ESS is not required. …”
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Should non-invasive prenatal testing be recommended for patients who achieve pregnancy with PGT? by Yunhao Liang, Meiyi Li, Jia Fei, Zhiheng Chen

“…Methods This was a retrospective study of the clinical outcomes of patients who underwent PGT and invasive or non-invasive pregnancy testing after euploid blastocyst transfer at our IVF centre between January 2017 and December 2022. …”
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Response of Bovine Cumulus–Oocytes Complexes to Energy Pathway Inhibition during In Vitro Maturation by Paulina Lipinska, Ewa Sell-Kubiak, Piotr Pawlak, Zofia Eliza Madeja, Ewelina Warzych

“…After in vitro fertilization, embryos were cultured to the blastocyst stage. Lipid droplets, metabolome, and lipidome were analyzed in oocytes and cumulus cells. mRNA expression of the selected genes was measured in the cumulus cells. …”
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cd26 Knockdown Negatively Affects Porcine Parthenogenetic Preimplantation Embryo Development by In-Sul Hwang, Joohyun Shim, Keon Bong Oh, Haesun Lee, Mi-Ryung Park

“…Inhibition of the cd26 expression did not affect cleavage but stopped development in the blastocyst stage. Additionally, the cd26 siRNA-treated blastocysts had significantly more apoptotic cells than the untreated blastocysts. …”
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Supplementing media with NAD+ precursors enhances the in vitro maturation of porcine oocytes by Charley-Lea POLLARD, Zamira GIBB, Azelle HAWDON, Aleona SWEGEN, Christopher G. GRUPEN

“…Supplementing the IVM medium with NA improved maturation and blastocyst formation while NAM supplementation improved cleavage rates compared with untreated controls. …”
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Biological and Clinical Significance of Mosaicism in Human Preimplantation Embryos by Ioanna Bouba, Elissavet Hatzi, Paris Ladias, Prodromos Sakaloglou, Charilaos Kostoulas, Ioannis Georgiou

“…Recently, groups of assisted reproduction experts have presented data that support the clinical use of mosaic preimplantation embryos at the blastocyst stage, previously excluded from transfer. …”
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Follicular Fluid: A Powerful Tool for the Understanding and Diagnosis of Polycystic Ovary Syndrome by Ana Teresa Brinca, Ana Cristina Ramalhinho, Ângela Sousa, António Hélio Oliani, Luiza Breitenfeld, Luís A. Passarinha, Eugenia Gallardo

“…These abnormalities originate a decrease of Metaphase II (MII) oocytes and decreased rates for fertilization, cleavage, implantation, blastocyst conversion, poor egg to follicle ratio, and increased miscarriages. …”
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Improvement of a twice collection method of mouse oocytes by surgical operation by Rei INOUE, Kana HARADA, Sayaka WAKAYAMA, Masatoshi OOGA, Teruhiko WAKAYAMA

“…When oocytes were collected from those mice a second time, the average number of oocytes was reduced by nearly 1/3. However, the blastocyst and offspring rates of those oocytes after IVF or ICSI were the same as those of the control regardless of the recovery day period. …”
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Clinical implications of first-trimester ultrasound dating in singleton pregnancies obtained through in vitro fertilization. by Agnese Maria Chiara Rapisarda, Edgardo Somigliana, Chiara Dallagiovanna, Marco Reschini, Maria Grazia Pezone, Veronica Accurti, Giuditta Ferrara, Nicola Persico, Simona Boito

“…This discrepancy persisted when subgroups were analyzed comparing different IVF procedures (conventional IVF versus ICSI, cleavage versus blastocyst transfer, frozen versus fresh transfer). …”
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Atomic force spectroscopy‐based essay to evaluate oocyte postovulatory aging by Alice Battistella, Laura Andolfi, Michele Zanetti, Simone Dal Zilio, Marco Stebel, Giuseppe Ricci, Marco Lazzarino

“…Interestingly, the oocyte mechanics correlates as well with the yield of embryo formation, evaluated up to the blastocyst formation stage. These results demonstrate that minimally invasive mechanical measurements are very sensitive to the aging of the oocyte and can be used as a label‐free method to detect the age of the postovulatory oocytes.…”
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Chlormadinone acetate in progestin‐primed ovarian stimulation does not negatively affect clinical results by Sena Shibasaki, Hiromitsu Hattori, Masae Koizumi, Satoko Nagaura, Mayumi Toya, Hideki Igarashi, Koichi Kyono

“…There was no cycle with premature LH surge in the PPOS group. Fertilization and blastocyst formation rates did not differ significantly between groups. …”
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The effects of type III antifreeze protein and human heat shock protein 70 added to the vitrification medium of mouse embryos on in vitro embryonic development rates by Mustafa Bodu, Mehmet Bozkurt Ataman

“…Four embryos, which progressed to the full blastocyst stage, were taken from each group and stained with the Hoeschst 33258 and propidium iodine (PI) dyes to determine the inner cell mass (ICM), trophectoderm (TE) and total cell numbers. …”
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3D Liquid Marble Microbioreactors Support <i>In Vitro</i> Maturation of Prepubertal Ovine Oocytes and Affect Expression of Oocyte-Specific Factors by Daniela Bebbere, Stefano Mario Nieddu, Federica Ariu, Davide Piras, Sergio Ledda

“…LM and 4W groups showed similar IVM and IVF rates, while in vitro development to blastocyst stage approached significance (4W: 14.1% vs. …”
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Beneficial Effect of Polysaccharide Gel Made of Xanthan Gum and Locust Bean Gum on Bovine Oocytes by Shunsuke Hara, Yuki Inoue, Sogo Aoki, Keisuke Tanaka, Koumei Shirasuna, Hisataka Iwata

“…The gel culture system improved the rate of development to the blastocyst stage. The oocytes that matured on the gel contained high lipid contents and F-actin formation, and the resultant 8-cell stage embryos had low DNA methylation levels compared to their plate counterparts. …”
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Regulation of X-linked gene expression during early mouse development by by Wang, Feng, Shin, JongDae, Shea, Jeremy M, Yu, Jun, Bošković, Ana, Byron, Meg, Zhu, Xiaochun, Lawrence, Jeanne B, Torres, Eduardo M, Zhu, Lihua J, Rando, Oliver J, Bach, Ingolf, Shalek, Alexander K, Regev, Aviv

“…Our results reveal crucial roles of Rlim for the maintenance of high Xist RNA levels, Xist clouds and X-silencing in female embryos at blastocyst stages, while initial Xist expression appears Rlim-independent. …”
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Human Naive Pluripotent Stem Cells Model X Chromosome Dampening and X Inactivation by Sahakyan, Anna, Kim, Rachel, Chronis, Constantinos, Sabri, Shan, Bonora, Giancarlo, Theunissen, Thorold W., Kuoy, Edward, Langerman, Justin, Clark, Amander T., Plath, Kathrin, Jaenisch, Rudolf

“…Naive human embryonic stem cells (hESCs) can be derived from primed hESCs or directly from blastocysts, but their X chromosome state has remained unresolved. …”
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Elucidation of cryotolerancein preimplantation murine embryos using confocal microscopy and developmental study / Razif Dasiman by Dasiman, Razif

“…The results demonstrate that in vivo embryonic survival until the hatched blastocyst stage was more adversely affected by slow freezing compared to control and vitrified embryos. …”
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The protective role of tocotrienol on corticosterone induced oxidative stress during preimplantation embryonic development in mice / Shahidee Zainal Abidin by Zainal Abidin, Shahidee

“…It was found that oral supplementation of 90 mg kg" BW of TCT in CORT-treated mice were able to normalize the number of fragmented embryos and improve the number of embryos that reach the blastocyst stage. No DNA damage was noted in all CORTtreated groups supplemented with TCT. …”
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